LOCUS       .                       7205 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             760..4345
                     /translation="FRNSLKMLLTGGKSSRKNRSSDGGSEEPPDRRQSSVDSRQSRSGQ
                     GGISTESDCAFEPDYAVPPLPVSEGMQHIRIMEGMSRSLPSSPLLTHQSISVRLQPVKK
                     LTAPLRKAKFVESPRIPESELGSPTLTSAQKLDVDAYCPGDAEQELGPPPSVDEAANTL
                     MTRLGFLLGEKVTEVQPGDQYSMEVQDENQTSAITQRISPCSTLTSSTASPPASSPCST
                     LPPISTNATAKDCSYGAVTSPTSTLESRDSGIIATLTSYSENVERTKYAGESSKELGSG
                     GNIKPWQSQKSSMDSCLYRVDENMTASTYSLNKIPERNLETVLSQSVQSIPLYLMPRPN
                     SVAATSSAHLEDLAYLDEQRHTPLRTSLRMPRQSMGGARTQQDLRVRFAPYRPPDISLK
                     PLLFEVPSITTESVFVGRDWVFHEIDAQLQSSNASVNQGVVIVGNIGFGKTAIISRLVA
                     LSCHGTRMRQIASDSPHASPKHVDANRELPLTQPPSAHSSITSGSCPGTPEMRRRQEEA
                     MRRLASQVVAYHYCQADNAYTCLVPEFVHNVAALLCRSPQLTAYREQLLREPHLQSMLS
                     LRSCVQDPMASFRRGVLEPLENLHKERKIPDEDFIILIDGLNEAEFHKPDYGDTIVSFL
                     SKMIGKFPSWLKLIVTVRTSLQEITKLLPFHRIFLDRLEENEAIDQDLQAYILHRIHSS
                     SEIQNNISLNGKMDNTTFGKLSSHLKTLSQGSYLYLKLTFDLIEKGYLVLKSSSYKVVP
                     VSLSEVYLLQCNMKFPTQSSFDRVMPLLNVAVASLHPLTDEHIFQAINAGSIEGTLEWE
                     DFQQRMENLSMFLIKRRDMTRMFVHPSFREWLIWREEGEKTKFLCDPRSGHTLLAFWFS
                     RQEGKLNRQQTIELGHHILKAHIFKGLSKKVGVSSSILQGLWISYSTEGLSMALASLRN
                     LYTPNIKVSRLLILGGANINYRTEVLNNAPILCVQSHLGYTEMVALLLEFGANVDASSE
                     SGLTPLGYAAAAGYLSIVVLLCKKRAKVDHLDKNGQCALVHAALRGHLEVVKFLIQCDW
                     TMAGQQQGVFKKSHAIQQALIAAASMGYTEIVSYLLDLPEKDEEEVERAQINSFDSLWG
                     ETALTAAAGRGKLEVCRLLLEQGAAVAQPNRRGAVPLFSTVRQGHWQIVDLLLTHGADV
                     NMADKQGRTPLMMAASEGHLGTVDFLLAQ"
                     /note="isoform 2 is encoded by transcript variant 1;
                     putative ankyrin-repeat containing protein; rolling pebbles
                     homolog B; protein TANC2; tetratricopeptide repeat, ankyrin
                     repeat and coiled-coil domain-containing protein 2"
                     /note="color: #993366"
                     /db_xref="{'GI': '2043521339', 'CCDS': 'CCDS92376.1',
                     'GeneID': '26115', 'HGNC': 'HGNC:30212', 'MIM': '615047'}"
                     /gene="TANC2"
                     /product="protein TANC2 isoform 2"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /transl_table=1
                     /codon_start=1
                     /protein_id="NP_001381927.1"
                     /label="TANC2"
     CDS             5525..6385
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /gene="bla"
                     /product="β-lactamase"
                     /transl_table=1
                     /codon_start=1
                     /label="AmpR"
     rep_origin      6556..7144
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      4683..5138
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   4468..4608
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     promoter        5420..5524
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          4346..4386
                     /label="Splicing Donor"
                     /note="color: noColor"
     misc_feature    4390..4423
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     CDS             727..753
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /codon_start=1
                     /label="HA"
                     /note="color: #cc99b2"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatctt ttcggaatag tctcaagatg
      781 ctgcttactg gtgggaaatc aagtcgtaaa aacaggtcaa gtgatggagg gagcgaggaa
      841 ccaccggatc gaagacagtc aagtgtagac tctcgccaaa gccgctctgg gcaaggtggc
      901 atctccacag aaagcgactg tgcttttgag ccagactacg ctgtcccgcc acttccagtg
      961 agtgaaggta tgcagcacat tcggattatg gagggcatgt ctcgctctct tccatcctcc
     1021 cccttactca cacatcagtc tatcagtgtt cggctccagc ctgtgaagaa gttaactgcc
     1081 cctctgagga aggcaaagtt tgttgagagc ccacgaattc cagaatccga gcttggctca
     1141 ccaaccctca cttcagctca gaaactggac gtggatgcat actgccctgg tgatgctgaa
     1201 caggagcttg gcccccctcc atctgtagat gaggcagcaa acacactcat gactcgtctg
     1261 ggtttcctcc ttggagagaa agtcacagaa gttcagccag gtgaccaata cagcatggaa
     1321 gtacaggatg aaaatcagac ctcagcaatc acccagcgga taagtccttg ttccactctg
     1381 actagcagca ctgcctctcc accagccagt agcccctgct ctacactccc acccatcagt
     1441 acaaatgcaa ctgccaagga ctgcagctat ggggctgtta ctagtccaac ctctaccctt
     1501 gaaagcagag atagtggcat cattgctaca ttaacaagct attctgaaaa tgtggaacgc
     1561 acaaaatatg ctggggaaag cagtaaagaa ttaggatctg gaggaaacat aaaaccttgg
     1621 cagtctcaaa aatccagcat ggactcctgt ttgtatcgag tagatgaaaa catgactgct
     1681 tccacctata gtctgaataa gatcccagag agaaatttgg aaacagtgtt atctcaatca
     1741 gtacagtcta ttcctttgta tctcatgcca cggccaaatt cagtagcagc caccagctct
     1801 gcccacttgg aagaccttgc ttacctggat gagcagagac atacaccttt gagaacttct
     1861 ctacgaatgc caagacagag catgggtggt gctcgcacac agcaggattt aagagttcgc
     1921 tttgcacctt ataggcctcc agatatctcc ttgaagcctc tgctctttga agtgcccagc
     1981 atcactacag agtcagtgtt tgttggccga gattgggttt tccacgaaat agatgctcaa
     2041 cttcaaagtt caaatgccag cgtgaaccaa ggagtagtga ttgtgggaaa cattggattc
     2101 ggcaaaactg ccatcatctc cagactggtg gccctcagct gccatggtac aaggatgaga
     2161 cagatcgcct cagacagccc acatgcctcc cccaaacatg tggatgccaa cagagagctg
     2221 ccactcacac agccaccttc agcccactca tctatcacca gtggaagctg cccaggaact
     2281 ccggaaatgc gcaggcggca ggaggaggct atgcgaagac tagcctcgca ggtggttgcc
     2341 tatcactatt gtcaagcaga taatgcctac acttgcttgg tgccagaatt tgtccacaat
     2401 gttgctgcct tgctctgccg ctcacctcag ctgacagcct atcgggagca gcttcttcgg
     2461 gaacctcacc tgcagagcat gctgagcctt cgttcctgtg ttcaagaccc catggcctcc
     2521 ttccggaggg gagttctgga gccactagaa aatctccata aagagagaaa aatcccagat
     2581 gaagatttca tcattttaat tgatggatta aatgaagcag aatttcacaa accggattat
     2641 ggggatacaa ttgtatcgtt tctgagtaaa atgatcggaa agtttccttc ttggctcaaa
     2701 ctaattgtaa cagttaggac cagtttacag gaaattacca agctgctgcc tttccatagg
     2761 atttttttgg atcgactaga agagaatgaa gccatagacc aggacctgca ggcttacatc
     2821 ctgcaccgga tacacagcag ctcagagatc cagaataaca tttcacttaa tggcaaaatg
     2881 gacaatacta catttggcaa actcagttct catctcaaga ccctcagtca agggtcctat
     2941 ctatatctga aacttacatt tgacctcata gagaaaggct atctagtgtt aaagagctct
     3001 agctacaaag tagttcctgt ttcgctctca gaggtttatt tactccagtg caatatgaag
     3061 ttcccaaccc agtcttcctt tgaccgggtg atgcctctcc tgaatgtggc agtggcctct
     3121 ctccacccac tgactgatga gcatatcttc caggccatca atgctgggag cattgaaggc
     3181 acactagaat gggaggattt tcagcagaga atggagaacc tctccatgtt cctaatcaag
     3241 cgcagagaca tgactcgtat gtttgtacat ccttcttttc gagaatggct tatctggaga
     3301 gaagaaggag agaaaaccaa atttctctgt gatccgagga gtggtcacac gttacttgcc
     3361 ttctggtttt cccgccaaga gggaaaacta aaccgacagc agactattga actgggacat
     3421 cacatcctca aagcacacat ttttaagggt ttgagtaaaa aagttggtgt atcatcctcc
     3481 atcctccaag gtctctggat ctcttatagc acagaaggtc tttccatggc actggcgtct
     3541 ttacgaaatc tctacactcc aaatataaag gtcagccgac tgctgatttt gggaggtgcc
     3601 aatattaatt accggacaga ggttttaaat aatgctccaa ttctatgtgt tcagtcccat
     3661 cttggttaca cagaaatggt agccctgctg ctggagttcg gggccaacgt ggatgcctct
     3721 tctgaaagtg gcctgactcc cttgggatat gctgcagcag cagggtacct gagcattgtg
     3781 gtgctgctgt gcaagaaacg ggccaaggtg gatcatttgg ataagaacgg gcagtgtgct
     3841 ttggttcatg ctgcactccg aggtcatctg gaggttgtca agtttttgat tcagtgtgac
     3901 tggacgatgg ccggccagca gcaaggagta tttaagaaga gccatgccat ccaacaggcc
     3961 ctcattgctg cagccagcat gggttatact gagattgtct cctacctact tgatcttcca
     4021 gaaaaagatg aagaggaagt agagcgagca cagatcaaca gctttgacag tctctgggga
     4081 gagacagccc taacagctgc agccggaagg ggcaaactgg aggtgtgccg tttgctcttg
     4141 gaacaagggg cggcagtggc ccagccaaac cgccgaggag cagtgccact attcagcaca
     4201 gtgcgccagg gccactggca gattgttgat cttttactca cccatggagc tgatgtcaac
     4261 atggcagaca agcagggccg cactcccctg atgatggctg cttccgaagg ccatctagga
     4321 accgtggact ttctgcttgc acaaggtaag tatcaaggtt acaagacagg tttaaggaga
     4381 ccaataagct attattcgta taggatactt tatacgaagt tattaagtta tggagaccgg
     4441 tgtggcacgt gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct
     4501 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
     4561 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc
     4621 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
     4681 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4741 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     4801 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     4861 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
     4921 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     4981 tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt
     5041 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     5101 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
     5161 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     5221 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     5281 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     5341 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     5401 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5461 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5521 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5581 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5641 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5701 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5761 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5821 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5881 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5941 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6001 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6061 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6121 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6181 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6241 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6301 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6361 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6421 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6481 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6541 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6601 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6661 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     6721 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6781 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6841 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6901 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6961 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7021 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7081 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7141 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7201 catgt
//
