LOCUS       .                       7421 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1493..4125
                     /gene="TANC2"
                     /product="protein TANC2 isoform 2"
                     /translation="ASIALMDKEGLTALSWACLKGHLSVVRSLVDNGAATDHADKNGRT
                     PLDLAAFYGDAEVVQFLVDHGAMIEHVDYSGMRPLDRAVGCRNTSVVVTLLKKGAKIGC
                     QTLPSRPRGPATWAMATSKPDIMIILLSKLMEEGDMFYKKGKVKEAAQRYQYALKKFPR
                     EGFGEDLKTFRELKVSLLLNLSRCRRKMNDFGMAEEFATKALELKPKSYEAYYARARAK
                     RSSRQFAAALEDLNEAIKLCPNNREIQRLLLRVEEECRQMQQPQQPPPPPQPQQQLPEE
                     AEPEPQHEDIYSVQDIFEEEYLEQDVENVSIGLQTEARPSQGLPVIQSPPSSPPHRDSA
                     YISSSPLGSHQVFDFRSSSSVGSPTRQTYQSTSPALSPTHQNSHYRPSPPHTSPAHQGG
                     SYRFSPPPVGGQGKEYPSPPPSPLRRGPQYRASPPAESMSVYRSQSGSPVRYQQETSVS
                     QLPGRPKSPLSKMAQRPYQMPQLPVAVPQQGLRLQPAKAQIVRSNQPSPAVHSSTVIPT
                     GAYGQVAHSMASKYQSSQGDIGVSQSRLVYQGSIGGIVGDGRPVQHVQASLSAGAICQH
                     GGLTKEDLPQRPSSAYRGGVRYSQTPQIGRSQSASYYPVCHSKLDLERSSSQLGSPDVS
                     HLIRRPISVNPNEIKPHPPTPRPLLHSQSVGLRFSPSSNSISSTSNLTPTFRPSSSIQQ
                     MEIPLKPAYERSCDELSPVSPTQGGYPSEPTRSRTTPFMGIIDKTARTQQYPHLHQQNR
                     TWAVSSVDTVLSPTSPGNLPQPESFSPPSSISNIAFYNKTNNAQNGHLLEDDYYSPHGM
                     LANGSRGDLLERVSQASSYPDVKVARTLPVAQAYQDNLYRQLSRDSRQGQTSPIKPKRP
                     FVESNV"
                     /note="isoform 2 is encoded by transcript variant 1;
                     putative ankyrin-repeat containing protein; rolling pebbles
                     homolog B; protein TANC2; tetratricopeptide repeat, ankyrin
                     repeat and coiled-coil domain-containing protein 2"
                     /note="color: #993366"
                     /db_xref="{'GI': '2043521339', 'CCDS': 'CCDS92376.1',
                     'GeneID': '26115', 'HGNC': 'HGNC:30212', 'MIM': '615047'}"
                     /gene_synonym="IDDALDS; rols; ROLSA"
                     /transl_table=1
                     /protein_id="NP_001381927.1"
                     /codon_start=1
                     /label="TANC2"
     CDS             288..1318
                     /product="site-specific recombinase"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /transl_table=1
                     /protein_id=""
                     /codon_start=1
                     /label="Cre"
     CDS             5741..6601
                     /gene="bla"
                     /product="β-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /transl_table=1
                     /codon_start=1
                     /label="AmpR"
     rep_origin      6772..7360
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     rep_origin      4899..5354
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     terminator      4210..4641
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   4684..4824
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     promoter        5636..5740
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     CDS             4129..4194
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /transl_table=1
                     /codon_start=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     protein_bind    1367..1400
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /label="lox2272-JTZ17"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtgcctcc
     1501 attgctctta tggacaaaga aggattgaca gccctcagct gggcttgttt gaagggccat
     1561 ctctcagtag tacgttctct ggtggataac ggagctgcca cagaccatgc tgacaagaat
     1621 ggccgtaccc cactggatct ggcagctttc tatggcgatg ctgaggtggt ccagttcctg
     1681 gtagatcatg gggccatgat cgagcacgtt gactacagtg gaatgcgccc tttggatagg
     1741 gcagtggggt gccggaacac ttctgttgtt gtcactcttc tgaagaaagg agccaagata
     1801 ggttgtcaga cgttaccgag tcgcccacga ggtccagcca catgggcgat ggccacctcc
     1861 aagccagaca tcatgatcat cctgttgagc aagctgatgg aagaggggga catgttttat
     1921 aagaaaggta aagtaaagga agctgcccag cgctaccagt acgccctgaa gaagttccct
     1981 agagaagggt ttggtgagga cttgaaaact ttccgggaac taaaggtgtc tctcctcctc
     2041 aacctctctc ggtgtcgcag gaaaatgaac gattttggaa tggcggagga atttgctact
     2101 aaggccctgg agctgaaacc gaaatcttat gaagcttact atgcgagagc aagggcaaaa
     2161 cgcagcagca gacagttcgc agcagcctta gaggacctga acgaggccat caagctgtgt
     2221 cccaacaacc gtgagatcca gagacttctg ctgagagtgg aagaagagtg tagacagatg
     2281 cagcagccac agcagccacc gccgccaccg cagcctcagc agcagttgcc ggaagaagca
     2341 gaacctgagc cacagcatga agacatatac tctgtacagg atatattcga ggaggagtac
     2401 ctggaacagg atgttgaaaa tgtttccatt ggcctccaga cagaggcccg gcccagccag
     2461 gggctcccgg tcatccagag cccaccctcc tctcccccgc atcgggactc agcctacatc
     2521 tccagctcac ctcttggctc tcatcaggtt tttgacttcc ggtccagtag ttctgtaggc
     2581 tctcccacta gacagaccta tcagtccacc tcacctgccc tttctccaac tcatcagaac
     2641 tcacattaca ggcctagccc accacacact tccccggctc atcagggagg atcttaccgt
     2701 ttcagccccc ctcctgtggg aggacagggc aaagaatacc caagccctcc cccttcccct
     2761 ctccggagag gccctcagta tcgggccagc cctccagctg aaagtatgag tgtctataga
     2821 tcccagtctg gttcacccgt gcgctatcag caggaaacaa gcgtcagtca gcttcctggc
     2881 agacccaaat ctccattatc caaaatggcc cagcggccct accagatgcc tcagctccct
     2941 gtggcagttc cccagcaagg gctcaggcta cagcctgcca aggcccagat tgtgagaagt
     3001 aaccagccca gcccagccgt ccattcaagc accgtcatcc ccacaggagc ctatggccaa
     3061 gtagcccatt caatggccag taaataccag tcttcacaag gagacatagg agtcagccag
     3121 agccggttgg tttatcaagg gtcaattggg ggaatcgtag gggatggaag gccggtgcag
     3181 catgtccaag ccagcctgag tgcaggcgcc atctgtcagc atggaggatt gaccaaagag
     3241 gatcttccac agcgaccttc ctcagcatac cgaggtggcg tgagatacag ccagacacca
     3301 cagatcggac gcagccagtc agcatcctat tacccagtct gtcactcaaa actagatctg
     3361 gagcgctcct ccagccaact aggttcccct gatgtgtcgc atttaatcag aagacctatc
     3421 agtgtcaacc ctaacgaaat caaaccgcac ccgccaactc ccaggccgtt gctgcattcc
     3481 caaagtgtag gccttcgctt ctctccatct agcaatagta tctcctccac ctccaaccta
     3541 actccgacct tccggccatc ttcttccatc cagcaaatgg agatcccact gaaacctgca
     3601 tatgagaggt catgtgacga gctgtcgcca gtgtctccaa ctcaaggagg ttaccccagt
     3661 gagcccaccc gatccaggac cacaccattc atggggatca tagataaaac agcacggact
     3721 cagcagtacc cccacctcca ccagcagaat cggacctggg cagtgtcatc tgtggacacc
     3781 gtcctcagtc ccacgtctcc aggcaacctg cctcagcctg agtccttcag tccaccatca
     3841 tccatcagca acattgcctt ttataacaaa accaacaatg cacagaatgg ccatttgctg
     3901 gaggacgatt attacagccc ccatgggatg ctggctaacg ggtctcgtgg agacctcttg
     3961 gagcgagtca gccaggcctc ctcctatccc gacgtgaagg tagctcggac tctacctgtg
     4021 gctcaggcat accaggacaa cctgtacagg cagctgtccc gagactctcg gcaagggcag
     4081 acatccccta tcaaaccaaa gagaccgttc gtggagtcta atgttaagga ctacaaagac
     4141 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtaagaa
     4201 ttctgatgac tcgagataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4261 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4321 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttagttc
     4381 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     4441 tgggcactga caattccgtg gtgtttattt gtgaaatttg tgatgctatt gctttatttg
     4501 taaccatcta gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     4561 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     4621 gagatgtggg aggtttttta aagatctgga gaccggtgtg gcacgtgcgg accgagcggc
     4681 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     4741 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     4801 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     4861 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     4921 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4981 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     5041 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5101 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5161 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5221 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc
     5281 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     5341 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     5401 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     5461 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     5521 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     5581 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5641 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5701 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5761 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5821 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5881 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5941 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     6001 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     6061 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     6121 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     6181 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     6241 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     6301 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6361 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6421 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6481 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6541 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6601 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6661 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6721 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6781 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6841 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6901 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6961 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     7021 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7081 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7141 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7201 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7261 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7321 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7381 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
