LOCUS       .                       6083 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             760..3223
                     /protein_id="NP_055558.2"
                     /translation="YPICTVVVDGLPSESSSSSYPGPVSVSEMSLLHALGPVQTWLGQE
                     LEKCGIDAMIYTRYVLSLLLHDSYDYDLQEQENDIFLGWEKGAYKKWGKSKKKCSDLTL
                     EEMKKQAAVQCLRSASDESSGIETLVEELCSRLKDLQSKQEEKIHKKLEGSPSPEAELS
                     PPAKDQVEMYYEAFPPLSEKPVCLQEIMTVWNKSKVCSYSSSSSSSTAPPASTDTSSPK
                     DCNSESEVTKERSSEVPTTVHEKTQSKSKNEKENKFSNGTIEEKPALYKKQIRHKPEGK
                     IRPRSWSSGSSEAGSSSSGNQGELKASMKYVKVRHKAREIRNKKGRNGQSRLSLKHGEK
                     AERNIHTGSSSSSSSGSVKQLCKRGKRPLKEIGRKDPGSTEGKDLYMENRKDTEYKEEP
                     LWYTEPIAEYFVPLSRKSKLETTYRNRQDTSDLTSEAVEELSESVHGLCISNNNLHKTY
                     LAAGTFIDGHFVEMPAVINEDIDLTGTSLCSLPEDNKYLDDIHLSELTHFYEVDIDQSM
                     LDPGASETMQGESRILNMIRQKSKENTDFEAECCIVLDGMELQGERAIWTDSTSSVGAE
                     GLFLQDLGNLAQFWECCSSSSGDADGESFGGDSPVRLSPILDSTVLNSHLLAGNQELFS
                     DINEGSGINSCFSVFEVQCSNSVLPFSFETLNLGNENTDSSANMLGKTQSRLLIWTKNS
                     AFEENEHCSNLSTRTCSPWSHSEETRSDNETLNIQFEESTQFNAEDINYVVPRVSSNYV
                     DEELLDFLQDETCQQNSRTLGEIPTLVFKKTSKLESVCGIQLEQKTENKNFETTQVCNE
                     SPHGDGYSS"
                     /gene="KIAA0232"
                     /db_xref="{'GI': '154689751', 'CCDS': 'CCDS43209.1',
                     'GeneID': '9778', 'HGNC': 'HGNC:28992', 'MIM': '619237'}"
                     /product="uncharacterized protein KIAA0232"
                     /codon_start=1
                     /transl_table=1
                     /label="KIAA0232"
                     /note="color: #993366"
     CDS             4403..5263
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /gene="bla"
                     /product="β-lactamase"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     rep_origin      5434..6022
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      3561..4016
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   3346..3486
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     promoter        4298..4402
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          3224..3264
                     /label="Splicing Donor"
                     /note="color: noColor"
     misc_feature    3268..3301
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     CDS             727..753
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /codon_start=1
                     /transl_table=1
                     /label="HA"
                     /note="color: #cc99b2"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatctt accctatctg tacagttgtt
      781 gtggatggtt tgccatctga aagctcctca agttcttatc caggccctgt gtctgtttct
      841 gaaatgtctc tgcttcatgc tttgggtcca gtgcagacct ggctgggaca agagctcgag
      901 aaatgtggca ttgatgccat gatttacact cggtatgtcc tcagtcttct gctgcatgac
      961 agctatgact acgacctgca ggaacaggag aatgacatct tcctgggctg ggaaaaagga
     1021 gcttataaga aatggggaaa gagtaagaaa aaatgttcag atctaactct agaagaaatg
     1081 aaaaaacagg ctgctgtcca gtgtcttcga tctgcttctg atgaaagctc tggtatcgag
     1141 actttagtgg aggagctctg ctccagactg aaagaccttc agagtaagca agaagagaag
     1201 attcacaaaa agttagaggg gtctccctct ccagaggcag aattatcccc tccagcaaag
     1261 gatcaagtgg aaatgtacta tgaagcattt ccaccacttt ctgagaaacc agtttgcctg
     1321 caagaaatca tgactgtgtg gaacaagtct aaagtctgtt cttactctag ctcttcttca
     1381 tcatccacag ccccaccagc tagcacagat acttcctctc ctaaggactg caacagtgaa
     1441 agtgaagtca ccaaggaaag aagcagtgaa gtacccacca ctgtgcatga gaaaacccag
     1501 agcaaaagca aaaacgagaa ggaaaacaaa tttagtaatg gcacaattga agaaaagcct
     1561 gctttgtaca aaaagcaaat ccgacataaa cctgaaggaa agattcgccc tcgctcgtgg
     1621 tcttctggct ccagtgaagc aggctcaagt tccagtggga atcagggaga attaaaagca
     1681 tccatgaagt atgttaaagt aagacacaag gcacgagaga ttcgaaacaa aaaagggcgg
     1741 aatgggcaaa gcaggctttc tttgaagcac ggtgaaaagg ctgaaaggaa cattcatact
     1801 ggaagtagta gcagtagcag cagtggttct gtcaaacagc tgtgcaagcg gggtaagaga
     1861 cctttaaaag aaatagggag aaaagatcct gggagcactg aaggaaaaga cctgtacatg
     1921 gagaatagaa aggacacaga gtataaagag gagcccttgt ggtacaccga gccaattgct
     1981 gaatattttg ttcctctgag cagaaaaagt aaactagaga ccacataccg aaacagacag
     2041 gatacaagtg atctgacatc agaggcagtg gaagaattgt ctgaatcagt gcatggtctt
     2101 tgtatcagca acaataatct tcataaaaca tacctcgcag caggtacttt cattgatggt
     2161 cattttgtag aaatgcctgc agttataaat gaggatattg acctcactgg gacctcatta
     2221 tgttctctac cagaggacaa taaatacctg gatgatattc atctatcaga attaacgcac
     2281 ttctatgaag tggatattga tcaatccatg ttggatcctg gtgcctcaga aacaatgcaa
     2341 ggagaaagtc ggattttgaa tatgattcga cagaaaagca aagagaacac agattttgag
     2401 gcagaatgtt gcatagtgtt agatggtatg gagttgcaag gggaacgtgc aatatggaca
     2461 gattctacca gctccgtagg tgctgagggc ttattcctgc aggaccttgg caatctggct
     2521 cagttttggg agtgctgttc atccagctcc ggtgatgctg atggggagag ttttggagga
     2581 gactctccag ttagactctc tcccatctta gacagcacag tgctcaattc acacctgctt
     2641 gctggcaatc aagagctctt ttcagatatt aatgaaggat ctggtataaa ctcttgtttt
     2701 tcagtgtttg aagtgcaatg cagtaattct gttttaccat tttcttttga aacactcaac
     2761 ttgggaaatg aaaatacaga ttctagtgct aatatgcttg ggaaaacaca gtctagattg
     2821 ctaatatgga ccaaaaatag tgcctttgaa gaaaatgaac actgttctaa tctttcaaca
     2881 agaacttgta gtccatggtc ccattcagaa gaaacacgtt cagacaatga aacattaaat
     2941 attcagtttg aagaatccac acagtttaat gccgaagata ttaattatgt agttcctaga
     3001 gtctcgtcaa attatgtaga tgaagaactt ctagattttt tgcaagatga aacttgccag
     3061 caaaacagta gaactttagg tgagattcct acattagttt tcaaaaaaac atctaaacta
     3121 gaatccgtct gtggtattca gctagaacaa aaaacagaaa acaaaaattt tgaaactaca
     3181 caagtatgta atgaaagtcc acatggagat ggctacagct caggtaagta tcaaggttac
     3241 aagacaggtt taaggagacc aataagctat tattcgtata ggatacttta tacgaagtta
     3301 ttaagttatg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga
     3361 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     3421 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
     3481 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     3541 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     3601 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     3661 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     3721 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     3781 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     3841 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     3901 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     3961 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     4021 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     4081 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     4141 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     4201 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     4261 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     4321 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4381 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4441 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4501 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4561 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4621 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     4681 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4741 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4801 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4861 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4921 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     4981 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     5041 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     5101 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     5161 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     5221 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     5281 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     5341 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5401 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5461 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5521 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5581 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5641 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5701 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5761 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5821 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5881 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5941 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6001 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6061 ttgctggcct tttgctcaca tgt
//
