LOCUS       .                       7299 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             415..4439
                     /translation="LSSGVETQPVPLDSSMSAVVQELYSELPVSVSRELHADPEPSVIP
                     DVKPGASSSLLSQNRALPLELQRTHVESCCEETYETLDHGSEPGRCGLVDSTAGGSVAS
                     GILDRAKRSESMEPKVFRDPGGQAGIIREPSEGAKEDPHQHSTAAEEKTSPSQEDLLMQ
                     SSKELSHVDLPEDFLRSKEGNVQITAETLLKSAEVQGMKVNGTKTDNNEGHKNGNVSKD
                     LSAGCGEFQEVDKIMTSDEVSETSTLVTPEPLTFVDPVLTEATPKEKECEELKSCPWLS
                     LPGNSAISNVDNGKEELCKPNLVCEADDNHQQLHGHHNEQPSSTHDSPTATSPLKENSE
                     VSCFTSDLSGPESRTISLENCGFEGGGLLKRSAEKTDSSYFYRGDDQGKNLASREENEE
                     RLLIPRSERGGPFLFNAREPEKEISGRCSGEKEPVVSPKENIHNNCIQDSLHTGNSSSL
                     MPNSFTEATEVMLNKNDLKITVHVQGNLTNPEDHKETFTNMSHPGGHSEESSFSSLMQI
                     EEAGQTTPVEPNILSKSFYTKDCNSLVSIQRNLEGNTQLNEASCNDFLFERKSIVSLMP
                     EDQISPVSEVLKPKQGTALLLPSPEFDYRPESEKVIQTSHDDIPLLDEQSIACEMNELS
                     CTNELVVNKVESECVLNQQVSLNSQEHANLPTDSLLHLNKEMPLATGRDAHQSHHPPLE
                     GRADVIADIQTIPIQTKIKDISPPGNQTCGASSNCPTLNIKPVSLERKKEMADSGTKAL
                     HSRLRSNKREAAGFPQVVSVIECHSVQSQDISSCHRVRKNVSQENMCSASAAFKSSKIS
                     LQVDNSLITKYENAFQHRDHCCQGTGHSVEKSSCKVSYTSQERELDGKETNGSLPGDKI
                     RNKMVAGLLNSGISNKTIHTSSSIKLSEEGLEGKEQDVSKETVFCKYNISDHAIQELNQ
                     TVNIPGPEKVLDQSPTVMFSSFKNVKSVETLDQKADEVLDCQSNQNRPDECKSEGQSAK
                     EMLSSDQRETVTEPHGEVNHNQKDLLVSSGSNNSLPCGSPKKCNLKGAFVKMSGCDEST
                     EGMVDIVYTDCSNKLAEGVLDVKASNLLDCGARQEKLAFQEDSRSTLSRRELDAAHTGT
                     TGQDSDFPVTAASTVDFLKIKKSCEENVCRSLKDCEMEKCPDSCAHEMESVADHEPNKR
                     ILGRVNLSLNDSHYGQQDKGTSLRETQEMTEGSRLEPNSEFGKESTFGISSKESMSCHD
                     ESSVSLRSLKSIEIMPSQENSETNVNSEETDLKNLCKPKDGEMLCENVKDCTVLPEMKE
                     IVSRDWSNSSDRDSVCTCVEKNACKACHPHENSSDRHLPLTVKTDIKVKGEETEEHQ"
                     /note="proline rich 14-like protein"
                     /note="color: #993366"
                     /db_xref="{'GI': '209969819', 'CCDS': 'CCDS13900.2',
                     'GeneID': '253143', 'HGNC': 'HGNC:28738'}"
                     /gene="PRR14L"
                     /product="protein PRR14L"
                     /gene_synonym="C22orf30"
                     /transl_table=1
                     /codon_start=1
                     /protein_id="NP_775837.2"
                     /label="PRR14L"
     CDS             5619..6479
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /gene="bla"
                     /product="β-lactamase"
                     /transl_table=1
                     /codon_start=1
                     /label="AmpR"
     rep_origin      6650..7238
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     rep_origin      4777..5232
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     repeat_region   4562..4702
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     promoter        5514..5618
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          4440..4480
                     /label="Splicing Donor"
                     /note="color: noColor"
     misc_feature    4484..4517
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     CDS             382..408
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /codon_start=1
                     /label="HA"
                     /note="color: #cc99b2"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     promoter        156..367
                     /note="core promoter for human elongation factor EF-1α"
                     /note="color: #ffffff"
                     /label="EF-1α core promoter"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgggca gagcgcacat cgcccacagt
      181 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg
      241 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
      301 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
      361 aacacagcgt acgccaccat gtacccttat gacgtacctg actatgctgg atctctgtca
      421 tctggagtag agactcagcc agttccactt gattcctcca tgtctgccgt ggtacaggaa
      481 ttatactctg aactcccagt aagtgtctcc agagagcttc atgctgaccc tgagccaagt
      541 gtgattccag atgtaaaacc tggagcctca agctctcttt taagtcagaa tagggcattg
      601 cccttggagc tgcagaggac tcatgtggag agttgttgtg aagaaaccta tgagaccttg
      661 gatcatggga gtgagcctgg gcgatgtggg ctagtggact ccacagcagg aggttctgtg
      721 gcatctggga tcttggatag ggcaaagaga agcgagagca tggagccaaa ggtcttcaga
      781 gatccagggg gccaggcagg aattataaga gaaccctctg agggagcaaa ggaagatcca
      841 catcaacatt ccacagctgc tgaagaaaag actagcccga gtcaggagga tctcctgatg
      901 cagtccagca aagaactttc acatgtggac ctccctgaag attttctaag gagcaaagaa
      961 ggaaatgtac agattacagc tgaaactctg ctaaaatccg ccgaagtaca aggtatgaag
     1021 gtcaatggga ctaagacgga taataatgaa ggacacaaaa atggcaatgt gagtaaagat
     1081 ctctcagctg gatgcggtga attccaagaa gtagacaaaa tcatgaccag tgatgaggtt
     1141 tcagaaacca gcacattagt taccccagaa cctttaacct ttgtggaccc tgtattaaca
     1201 gaagcaactc ctaaagaaaa agaatgtgaa gaattaaaaa gttgtccttg gttgtcatta
     1261 ccaggaaaca gtgccatttc taatgtggac aatgggaagg aagagttgtg taaaccaaac
     1321 ctggtctgtg aagcagatga caatcaccaa cagcttcatg gccaccataa tgaacaaccc
     1381 agttctacac atgatagtcc cacagccaca agccctttga aagaaaattc tgaagtttca
     1441 tgtttcacat cagacttgtc tggtccagaa tccagaacaa tatccttaga aaactgtggt
     1501 tttgaaggtg gtggtttgct aaagagatct gctgaaaaga cagacagttc ttatttttat
     1561 aggggggatg atcaagggaa gaacttggct tctagagaag aaaatgagga acggcttttg
     1621 attcctagga gtgaaagagg tggacctttt ctttttaatg ccagggaacc agaaaaggag
     1681 attagtggtc gttgttctgg tgaaaaagag cctgttgttt ctccaaagga aaatatccac
     1741 aataactgca ttcaagacag tctccataca gggaactcta gttctttaat gcccaattct
     1801 tttactgaag ccacagaagt aatgttaaat aaaaatgatt tgaaaattac tgtacacgtt
     1861 caaggtaact tgacaaaccc tgaggaccat aaagaaactt ttactaatat gagccatcct
     1921 ggtggacact ctgaagaaag cagtttttcc tccttgatgc agattgaaga ggcaggacag
     1981 acaacccctg tagagcccaa tatattaagt aaatcttttt acactaaaga ctgtaactcc
     2041 ttagtcagca tccagagaaa tctggaaggc aacacccagt taaatgaagc atcatgtaat
     2101 gattttctgt ttgaaagaaa atccattgtg agtttaatgc cagaggatca aataagtcct
     2161 gtaagtgagg tattaaaacc caagcaaggt actgctctgt tgctaccatc cccagaattt
     2221 gattacagac ctgagtcaga aaaagttata cagacctcac atgatgatat tccactttta
     2281 gatgaacaga gcatagcctg tgagatgaat gaactttctt gtaccaatga actggttgta
     2341 aacaaagtag aaagtgaatg tgttttaaat caacaagtgt cccttaattc tcaagaacat
     2401 gcaaatttgc caactgactc tctactgcat ttaaacaaag agatgccttt agcaacaggc
     2461 agagatgccc atcagagcca tcaccctcca ttagagggta gagcagatgt cattgctgat
     2521 atacaaacca ttcccattca gacaaaaata aaagacatct ctccaccagg taaccaaacc
     2581 tgtggtgcct cttcaaactg tcccacctta aacatcaaac cagtaagcct agagagaaaa
     2641 aaggaaatgg ctgattcagg aacaaaagct ctacattcca ggctgcgctc aaataagaga
     2701 gaagcagctg gctttcctca agtggtctct gtcatagaat gtcacagcgt tcaatctcag
     2761 gatatctcta gctgtcatcg tgtaagaaaa aatgtatccc aggaaaacat gtgttctgct
     2821 tctgctgctt tcaagtccag caaaatcagc ctgcaagttg ataactcttt gataacaaaa
     2881 tatgaaaatg catttcagca ccgtgatcac tgctgccaag gaacaggaca ctctgtggaa
     2941 aaaagcagct gtaaagtgag ttacacatca caggaaaggg aacttgatgg gaaagaaacg
     3001 aatggcagcc ttccaggaga taagatcaga aacaaaatgg tagcaggctt gttaaatagt
     3061 ggaatttcaa acaaaaccat tcacacctcc agtagcatca aactcagtga ggaagggctg
     3121 gaaggaaagg agcaggatgt atccaaagaa actgtatttt gtaagtataa catctctgat
     3181 catgctatac aagaactaaa ccagactgta aacattccag gtcctgaaaa agtgttggac
     3241 cagtctccta ctgttatgtt ctccagtttt aaaaatgtaa aatcagttga aacactcgat
     3301 cagaaggcag atgaagtcct tgactgtcag agtaaccaaa acagaccaga tgaatgcaaa
     3361 agtgaaggtc agtcagccaa ggagatgcta agtagtgacc agagagagac tgtcaccgag
     3421 cctcacgggg aggtaaacca caaccaaaag gatctgctgg tcagctcagg cagtaataac
     3481 tcactacctt gtggtagtcc aaagaaatgc aatttgaaag gagcctttgt caagatgtct
     3541 ggttgtgatg agtccacaga aggtatggta gacatcgtct acacggactg tagtaataag
     3601 cttgcagaag gtgtgctgga cgtgaaggca tctaatctac tggattgtgg tgcaaggcaa
     3661 gagaaactgg catttcaaga ggactccagg agtaccttgt ctcggagaga actggatgct
     3721 gcacatacag gaacaactgg tcaggattca gatttcccag ttactgctgc ttctacagtg
     3781 gactttctca aaataaaaaa atcatgtgaa gaaaacgtat gcagatcttt aaaagactgt
     3841 gaaatggaaa agtgtccaga ctcttgtgcc catgagatgg agtctgttgc agatcatgaa
     3901 ccaaataaaa gaatattggg cagagtaaat ttgtctttaa atgatagcca ttatggacag
     3961 caagataaag gaacatctct cagagaaaca caagaaatga ctgaaggatc aagactagaa
     4021 ccaaactctg agtttggcaa agaaagtacc tttggaattt cctcaaaaga gtcgatgtct
     4081 tgccatgatg aaagctctgt ttccctaaga agcctgaaat ccattgaaat aatgccttct
     4141 caagaaaatt cagaaactaa tgttaacagt gaagaaactg acctgaaaaa tctttgtaaa
     4201 ccaaaagatg gtgaaatgct ttgtgaaaat gtaaaggact gcacagtcct tcctgagatg
     4261 aaggaaatag tatcaagaga ttggagtaat tctagtgaca gagacagcgt atgtacctgt
     4321 gtggaaaaga atgcttgcaa agcttgtcac cctcacgaga attcctctga cagacatttg
     4381 cctttgacag tgaaaacaga tattaaagtg aaaggagaag aaaccgaaga gcatcagagg
     4441 taagtatcaa ggttacaaga caggtttaag gagaccaata agctattatt cgtataggat
     4501 actttatacg aagttattaa gttatggaga ccggtgtggc acgtgcggac cgagcggccg
     4561 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     4621 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     4681 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
     4741 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     4801 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4861 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4921 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4981 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     5041 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5101 cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct
     5161 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     5221 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     5281 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     5341 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     5401 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5461 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5521 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5581 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5641 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5701 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5761 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5821 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5881 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5941 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6001 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6061 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6121 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6181 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6241 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6301 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6361 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6421 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6481 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6541 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6601 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6661 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6721 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6781 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6841 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6901 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6961 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7021 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7081 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7141 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7201 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7261 tacggttcct ggccttttgc tggccttttg ctcacatgt
//
