LOCUS       .                       7216 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1493..3917
                     /product="protein PRR14L"
                     /note="proline rich 14-like protein"
                     /note="color: #993366"
                     /translation="GRLGYLTVGEQSEELVTRETGDGDPVSNISQTHFKCRGILNHAEK
                     QQSPEVLDYMLQKEEKYIRQQKAHTISQQCISSSLLLDDAQNQNQPKADKDESTMINEI
                     TLAKLAKDSIVAQTQKLEDQKEERLHHPLRKDTESCTSPCLLGAPRKAQDPSSAGCDQI
                     HGAFAKKGVLPLKKQPHRTCKKVSYQEQIIVGRKIGKIRSSAFLKSSSNPIPTKAHRLL
                     SLCTLSAPTRLEPETAPTKSLVSHIPKQMSTPCHPLRSLNFRKTTKESALLNKLSILAS
                     KLAPAMKTQKLRYRRCSSELLPMAKSYKRLRYKRLLDGFSSSTEQLNPYLAASGWDKRP
                     NSKPMALYSLESIKMTFIDLSNKMPSLLFGSEIFPVSFHVKSSSSDCTTESSRTFPEHC
                     APARLALGEALQCPSQPPKWTFSFFLSHGCPGMATFREDTGVHSQTHTQAPPQPPAPLQ
                     DYGGTAIVQTRADCSVLGLHTLLALCSPGCYRIWTKKRSFSSHMPTMQRLFMTQFTQGL
                     KGLRSPASIADKVFCSLPYSVGRVLSIWSQHGPSVCSFEISSLHSPHCKRQPSLGTTSS
                     HTMLPYVPLPGMEATYNTSGSQTRLEPPFPALVPKSCLVAESAVSKLLLSASEFQVRGL
                     DELDGVKAACPCPQSSPPEQKEAEPEKRPKKVSQIRIRKTIPRPDPNLTPMGLPRPKRL
                     KKKEFSLEEIYTNKNYKSPPANRCLETIFEEPKERNGTLISISQQKRKRVLEFQDFTVP
                     RKRRARGKVKVAGSFTRAQKAAVQSRELDALLIQKLMELETFFAKEEEQEQSSGC"
                     /db_xref="{'GI': '209969819', 'CCDS': 'CCDS13900.2',
                     'GeneID': '253143', 'HGNC': 'HGNC:28738'}"
                     /gene="PRR14L"
                     /transl_table=1
                     /codon_start=1
                     /protein_id="NP_775837.2"
                     /gene_synonym="C22orf30"
                     /label="PRR14L"
     CDS             5536..6396
                     /product="β-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /transl_table=1
                     /codon_start=1
                     /label="AmpR"
     rep_origin      6567..7155
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     rep_origin      4694..5149
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     terminator      4005..4436
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   4479..4619
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     promoter        5431..5535
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     CDS             3924..3989
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /transl_table=1
                     /codon_start=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     CDS             288..1318
                     /product="site-specific recombinase"
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /transl_table=1
                     /codon_start=1
                     /protein_id=""
                     /label="Cre"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     protein_bind    1367..1400
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /bound_moiety="Cre recombinase"
                     /label="lox2272-JTZ17"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggggacgac
     1501 tgggttactt aactgttggg gagcaatctg aggagttggt taccagagaa actggcgatg
     1561 gcgatcccgt gagcaacatc tctcagaccc attttaaatg ccgggggata cttaatcatg
     1621 ctgaaaaaca gcagagccct gaggttttgg actacatgtt gcagaaagaa gagaaatata
     1681 tacgtcaaca aaaagcacat acgatatcgc aacagtgcat atcatctagt ctgttgttag
     1741 atgatgcaca aaatcagaac caaccgaagg ctgacaaaga tgagtccacc atgatcaatg
     1801 aaatcaccct agcaaagctg gccaaggaca gcattgtggc acagactcag aagttagaag
     1861 accagaagga ggaaaggtta catcatccat taaggaagga cactgagtca tgcacaagtc
     1921 cttgccttct tggtgcccct cggaaagcac aagacccctc ctctgctggg tgtgatcaaa
     1981 tacatggtgc ctttgcgaag aaaggagttc ttcccttaaa gaagcagccc catcgaactt
     2041 gtaagaaagt ttcctatcag gagcaaatca ttgttgggag aaaaataggt aaaatcagaa
     2101 gttctgcctt tttaaagagt tcttccaatc ccatccccac aaaagcgcac agacttctca
     2161 gtttgtgtac tctgtctgca cctacacgat tagaacctga aacagcacct accaagagct
     2221 tggttagtca cataccaaag cagatgtcta caccgtgcca tcccttgagg agcctgaatt
     2281 ttaggaagac taccaaagaa tcagccttac taaacaagct gtccatcctt gcctccaaac
     2341 tggccccagc catgaagact cagaagctaa gataccgacg gtgttcctct gaacttcttc
     2401 caatggctaa aagctacaag cgcctcagat ataaaagact cctggacgga ttttcatcca
     2461 gcacagagca gctgaatcca tatttggcag ctagtggatg ggataagagg cctaacagta
     2521 agcccatggc actttattct ctcgaatcca tcaagatgac cttcatagat ttgagcaaca
     2581 agatgccgtc cctgctgttt ggttctgaaa tcttcccagt atcctttcat gtgaaatcat
     2641 ccagctcaga ttgcacgact gagtcctcaa ggacttttcc tgagcactgt gctccggcaa
     2701 ggcttgcctt aggagaggcc ctccagtgcc cgtctcaacc tcccaagtgg accttttctt
     2761 tcttcttgtc ccacggttgc cctgggatgg caacattcag ggaagacact ggcgtccata
     2821 gtcagaccca cactcaggct cctccccagc ctccagctcc tctccaagac tatggaggca
     2881 ctgccatagt ccagaccaga gcagactgct ctgtccttgg ccttcacaca cttctagcac
     2941 tttgttcccc aggatgttac cgaatctgga caaaaaaacg gagcttctcc agccacatgc
     3001 ctaccatgca gaggctcttc atgacccagt ttacacaggg cctgaaaggg ttacggtctc
     3061 cagcctccat agcagacaag gtcttctgtt ctctgcccta ctcggtgggc agagtcctat
     3121 ccatttggag ccagcatggt ccttctgtct gctccttcga aatctcttct cttcattccc
     3181 ctcactgcaa gcggcaacca agtctgggca ccacaagcag ccacaccatg ttaccatatg
     3241 tgcctcttcc aggcatggaa gctacatata acaccagcgg cagtcagacg aggctggagc
     3301 ctccattccc tgccttggta ccaaagtctt gcttggtagc agaatcagct gtcagcaagc
     3361 tcctgctttc agcctctgag ttccaggttc gtggattgga tgagctggat ggtgtgaaag
     3421 cagcatgccc ctgcccacag agcagccccc cagaacagaa agaggctgag ccagagaaga
     3481 ggccaaagaa agtctcacag attcgcatcc ggaaaaccat tcctaggcca gatcctaatc
     3541 ttacccccat gggccttcct cgacccaaaa ggttaaagaa gaaggagttt agtttagaag
     3601 agatatatac caacaagaat tataaatctc ctcctgcaaa caggtgttta gagaccatct
     3661 ttgaggaacc caaggaacga aatggtacac taatctcaat cagccaacag aagaggaagc
     3721 gagttctaga atttcaggat tttacagtcc cgcgaaagag gagagctcga ggcaaagtca
     3781 aggtggcagg cagctttacc agggcccaga aggcagctgt gcagagtcga gagctggatg
     3841 ctcttttgat acagaaacta atggaactgg agaccttctt tgccaaggaa gaggagcagg
     3901 aacaatcatc aggctgttgt acagactaca aagaccatga cggtgattat aaagatcatg
     3961 acatcgacta caaggatgac gatgacaagt aagaattctg atgactcgag ataatcaacc
     4021 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     4081 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     4141 cattttctcc tccttgtata aatcctggtt agttcttgcc acggcggaac tcatcgccgc
     4201 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     4261 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc atctagcttt atttgtgaaa
     4321 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     4381 acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagat
     4441 ctggagaccg gtgtggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt
     4501 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     4561 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     4621 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     4681 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4741 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4801 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4861 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     4921 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4981 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat
     5041 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     5101 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     5161 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     5221 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     5281 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     5341 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     5401 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5461 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5521 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5581 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5641 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5701 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5761 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     5821 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5881 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5941 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6001 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6061 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6121 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6181 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6241 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6301 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6361 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6421 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6481 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6541 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6601 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6661 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     6721 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6781 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6841 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6901 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6961 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7021 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7081 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7141 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7201 ccttttgctc acatgt
//
