LOCUS       .                       7444 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             382..408
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
                     /transl_table=1
                     /codon_start=1
                     /label="HA"
                     /note="color: #cc99b2"
     misc_feature    4629..4662
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     intron          4585..4625
                     /label="Splicing Donor"
                     /note="color: noColor"
     promoter        5659..5763
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   4707..4847
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     promoter        156..367
                     /note="core promoter for human elongation factor EF-1α"
                     /note="color: #ffffff"
                     /label="EF-1α core promoter"
     rep_origin      4922..5377
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     rep_origin      6795..7383
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     CDS             5764..6624
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /transl_table=1
                     /codon_start=1
                     /label="AmpR"
     CDS             415..4584
                     /note="mediator complex subunit 13 like; thyroid hormone
                     receptor-associated protein 2; thyroid hormone
                     receptor-associated protein complex 240 kDa component-like"
                     /note="color: #993366"
                     /product="mediator of RNA polymerase II transcription
                     subunit 13-like"
                     /translation="TAAANWVANGASLEDCHSNLFSLAELTGIKWRRYNFGGHGDCGPI
                     ISAPAQDDPILLSFIRCLQANLLCVWRRDVKPDCKELWIFWWGDEPNLVGVIHHELQVV
                     EEGLWENGLSYECRTLLFKAIHNLLERCLMDKNFVRIGKWFVRPYEKDEKPVNKSEHLS
                     CAFTFFLHGESNVCTSVEIAQHQPIYLINEEHIHMAQSSPAPFQVLVSPYGLNGTLTGQ
                     AYKMSDPATRKLIEEWQYFYPMVLKKKEESKEEDELGYDDDFPVAVEVIVGGVRMVYPS
                     AFVLISQNDIPVPQSVASAGGHIAVGQQGLGSVKDPSNCGMPLTPPTSPEQAILGESGG
                     MQSAASHLVSQDGGMITMHSPKRSGKIPPKLHNHMVHRVWKECILNRTQSKRSQMSTPT
                     LEEEPASNPATWDFVDPTQRVSCSCSRHKLLKRCAVGPNRPPTVSQPGFSAGPSSSSSL
                     PPPASSKHKTAERQEKGDKLQKRPLIPFHHRPSVAEELCMEQDTPGQKLGLAGIDSSLE
                     VSSSRKYDKQMAVPSRNTSKQMNLNPMDSPHSPISPLPPTLSPQPRGQETESLDPPSVP
                     VNPALYGNGLELQQLSTLDDRTVLVGQRLPLMAEVSETALYCGIRPSNPESSEKWWHSY
                     RLPPSDDAEFRPPELQGERCDAKMEVNSESTALQRLLAQPNKRFKIWQDKQPQLQPLHF
                     LDPLPLSQQPGDSLGEVNDPYTFEDGDIKYIFTANKKCKQGTEKDSLKKNKSEDGFGTK
                     DVTTPGHSTPVPDGKNAMSIFSSATKTDVRQDNAAGRAGSSSLTQVTDLAPSLHDLDNI
                     FDNSDDDELGAVSPALRSSKMPAVGTEDRPLGKDGRAAVPYPPTVADLQRMFPTPPSLE
                     QHPAFSPVMNYKDGISSETVTALGMMESPMVSMVSTQLTEFKMEVEDGLGSPKPEEIKD
                     FSYVHKVPSFQPFVGSSMFAPLKMLPSHCLLPLKIPDACLFRPSWAIPPKIEQLPMPPA
                     ATFIRDGYNNVPSVGSLADPDYLNTPQMNTPVTLNSAAPASNSGAGVLPSPATPRFSVP
                     TPRTPRTPRTPRGGGTASGQGSVKYDSTDQGSPASTPSTTRPLNSVEPATMQPIPEAHS
                     LYVTLILSDSVMNIFKDRNFDSCCICACNMNIKGADVGLYIPDSSNEDQYRCTCGFSAI
                     MNRKLGYNSGLFLEDELDIFGKNSDIGQAAERRLMMCQSTFLPQVEGTKKPQEPPISLL
                     LLLQNQHTQPFASLNFLDYISSNNRQTLPCVSWSYDRVQADNNDYWTECFNALEQGRQY
                     VDNPTGGKVDEALVRSATVHSWPHSNVLDISMLSSQDVVRMLLSLQPFLQDAIQKKRTG
                     RTWENIQHVQGPLTWQQFHKMAGRGTYGSEESPEPLPIPTLLVGYDK"
                     /protein_id="NP_056150.1"
                     /gene="MED13L"
                     /db_xref="{'GI': '44771211', 'CCDS': 'CCDS9177.1',
                     'GeneID': '23389', 'HGNC': 'HGNC:22962', 'MIM': '608771'}"
                     /transl_table=1
                     /codon_start=1
                     /gene_synonym="MRFACD; PROSIT240; THRAP2; TRAP240L"
                     /label="MED13L"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgggca gagcgcacat cgcccacagt
      181 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg
      241 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
      301 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
      361 aacacagcgt acgccaccat gtacccttat gacgtacctg actatgctgg atctactgcg
      421 gcagcgaact gggtggcgaa cggggcgagc ctggaggatt gtcactccaa cctcttttcg
      481 ctggctgaac tcacgggaat caaatggcgt aggtacaatt ttggagggca tggggactgt
      541 ggacccataa tttcagcccc agcccaagat gatccaattc tgttaagttt catccgctgt
      601 ctgcaagcta acctgctttg tgtatggcgt cgtgatgtca aaccagattg caaagagtta
      661 tggatattct ggtggggaga tgaacccaac ctagtgggtg taatacatca tgaactgcag
      721 gttgtggaag aaggactctg ggaaaatggc ctttcctatg aatgtaggac gctgctcttc
      781 aaagcgatcc acaatctgtt agaaaggtgc ctaatggata agaacttcgt taggattggg
      841 aaatggtttg tccgacccta cgaaaaggat gaaaagccag tcaacaaaag tgagcatttg
      901 tcctgtgctt tcacattctt tctgcatgga gaaagtaatg tatgcacaag tgtggagatt
      961 gcccagcacc agccaattta tttgatcaat gaggagcata tacacatggc tcagtcttca
     1021 cctgcaccat ttcaagtact ggtaagtcct tatggcttaa atgggacgct aacaggccaa
     1081 gcatacaaga tgtcagaccc agccactcgt aagttgattg aggaatggca gtatttctac
     1141 ccgatggtgc taaaaaagaa agaagaatcg aaagaggaag acgagttggg atatgatgat
     1201 gatttccctg tggcagttga agtaattgtt ggtggtgttc ggatggttta cccttcagca
     1261 tttgttttga tctctcagaa tgacatcccg gttcctcaga gtgttgccag tgctggaggc
     1321 cacattgcag ttgggcagca agggcttggt agtgtgaagg acccaagtaa ctgtgggatg
     1381 cctctgaccc ctcccacctc tccagaacag gctatcctag gtgagagtgg aggtatgcag
     1441 agtgctgcca gtcacctggt ttcccaagat ggagggatga taacgatgca cagtccaaag
     1501 agatcgggga agattcctcc aaaactccac aatcatatgg tccatcgagt ctggaaggaa
     1561 tgcatcctca acagaaccca gtccaagagg agccaaatgt caactccaac tcttgaagaa
     1621 gagcctgcta gcaatcctgc tacttgggat tttgtggatc caacccaaag agtcagctgt
     1681 tcttgttcca ggcataagct tttaaaacgt tgtgcagtcg ggcccaatcg acctcccaca
     1741 gtatctcaac cagggttcag tgcaggacca tcatcatctt catctttacc acctcctgct
     1801 tcttctaagc acaaaacagc agaaagacag gaaaaaggag acaagctgca aaagagaccc
     1861 ttaataccat ttcaccatag gccctctgtg gccgaagaat tatgcatgga gcaagataca
     1921 ccaggacaga aactagggtt ggcagggata gactcctcct tagaggtgtc tagcagtagg
     1981 aaatatgata agcaaatggc cgtgccttcc agaaatacaa gcaagcaaat gaatctgaat
     2041 cctatggatt cacctcattc ccctatatcc cctctgccac caacactcag ccctcagcca
     2101 cgaggtcagg aaacagagag tttggaccca ccatcggtcc ctgtgaatcc agccctttat
     2161 ggaaatggac tagaactcca gcagttgtct actctggatg acagaactgt cctcgtaggc
     2221 caaagactgc ctctcatggc agaggtcagc gagacagcct tatattgtgg gattaggccc
     2281 tcgaacccgg agtcatcaga aaagtggtgg catagttatc gtctcccacc cagtgatgat
     2341 gctgagttca ggcctccaga gctccagggt gagagatgtg atgccaaaat ggaggtaaac
     2401 tcagagagca ctgcattgca aagactctta gcacaaccta acaaacggtt taaaatctgg
     2461 caagacaaac agccccagtt gcagccactc cacttccttg acccattgcc tctatcacaa
     2521 caacctggag acagtttggg agaagtgaat gacccatata cctttgaaga tggtgacata
     2581 aaatacatct ttacagccaa caagaaatgc aaacaaggga cggagaaaga ttccctgaaa
     2641 aagaataagt cagaggatgg atttggtacc aaggatgtca ctacaccagg tcattccacg
     2701 ccggtgcctg atgggaaaaa tgccatgtct attttcagtt ctgctactaa aacagatgtc
     2761 cggcaggata atgctgctgg cagagctggc tccagtagcc ttacacaggt aacagatttg
     2821 gcaccttccc tgcatgactt agacaacatc tttgataatt ctgatgacga cgaacttggg
     2881 gctgtatcac ctgctctgcg ctcatcaaaa atgcctgcag ttgggacaga agaccgacct
     2941 cttgggaagg atggaagagc tgctgttcct tatccaccaa cagttgcaga cttgcaaagg
     3001 atgtttccca ctccaccatc tttggaacag catcctgcat tttctcctgt gatgaattat
     3061 aaagatggga tcagctcaga gacagtgaca gcattaggca tgatggagag ccctatggtc
     3121 agtatggttt caacacaact cacagaattc aaaatggaag tggaagatgg attaggaagt
     3181 cccaagcccg aggaaattaa ggacttttca tatgtgcaca aagttccatc ctttcaacct
     3241 tttgtgggat cctccatgtt tgctccactg aagatgttgc cgagccattg tttgctacct
     3301 ctgaagatac ctgatgcctg tctgtttcgg ccttcatggg caattcctcc taaaattgaa
     3361 caactgccca tgccccctgc agccactttc attagagatg gctacaataa cgtgcctagt
     3421 gttgggagcc tagcagatcc agactatctg aacacaccac agatgaacac acccgtgacg
     3481 ttgaacagcg ctgccccagc cagcaatagt ggggcaggag tcctaccatc tccagcaacc
     3541 cctcgcttct ctgtccccac accacgaacc cccaggaccc caagaactcc cagaggtggg
     3601 ggcactgcca gtggtcaagg gtctgttaag tatgatagca ccgatcaagg atcaccagcc
     3661 tccaccccct ctactacacg gcccctcaac tctgtggagc ccgccaccat gcagccaatt
     3721 cccgaagccc acagcctcta tgttaccctg attctctccg attccgtgat gaatatcttt
     3781 aaagacagaa actttgacag ctgttgcatc tgtgcctgca acatgaacat caaaggggcg
     3841 gatgtcgggc tttacatccc cgattcttcc aatgaggacc agtaccgctg tacctgtggg
     3901 tttagtgcga ttatgaaccg caaacttggc tacaattcag gactcttcct tgaagatgag
     3961 ttggatattt ttgggaagaa ttctgatatt ggtcaggctg cagagaggcg cttaatgatg
     4021 tgtcagtcca ccttccttcc tcaggtggaa ggaaccaaaa aaccccagga gccacccata
     4081 agccttctcc tcctcctcca gaatcaacac acacaacctt ttgcttcact gaatttcctg
     4141 gactacattt cctctaacaa tcgccaaact cttccctgtg taagctggag ttatgaccgg
     4201 gtgcaagcag ataataatga ttactggacg gaatgcttta atgcgttgga gcaggggcgg
     4261 cagtatgtgg ataaccccac tggtggaaaa gtggacgaag ctctggtgag aagtgccact
     4321 gtgcactctt ggcctcacag caatgtgctg gacatcagca tgctctcctc ccaggatgtg
     4381 gttcgtatgc tgttgtccct gcagcccttt ctccaagatg ccatccaaaa gaagcgcacg
     4441 ggcaggacct gggagaacat ccagcatgtg cagggaccac tcacttggca gcagttccat
     4501 aaaatggcag gacggggaac ctacggttcg gaagaatctc ctgagccgtt gcccatcccc
     4561 actctgctgg taggctatga caaggtaagt atcaaggtta caagacaggt ttaaggagac
     4621 caataagcta ttattcgtat aggatacttt atacgaagtt attaagttat ggagaccggt
     4681 gtggcacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc
     4741 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt
     4801 tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg
     4861 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
     4921 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4981 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     5041 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     5101 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
     5161 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     5221 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta
     5281 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     5341 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc
     5401 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     5461 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     5521 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     5581 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     5641 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5701 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5761 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5821 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5881 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     5941 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6001 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6061 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     6121 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     6181 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     6241 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     6301 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     6361 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     6421 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     6481 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     6541 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     6601 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6661 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6721 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6781 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6841 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6901 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     6961 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7021 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7081 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7141 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7201 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7261 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7321 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7381 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     7441 atgt
//
