LOCUS       .                       7248 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    1367..1400
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /label="lox2272-JTZ17"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     CDS             3956..4021
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /codon_start=1
                     /transl_table=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     promoter        5463..5567
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     repeat_region   4511..4651
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     terminator      4037..4468
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     rep_origin      4726..5181
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     rep_origin      6599..7187
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     CDS             5568..6428
                     /gene="bla"
                     /product="β-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     CDS             288..1318
                     /product="site-specific recombinase"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /protein_id=""
                     /codon_start=1
                     /transl_table=1
                     /label="Cre"
     CDS             1493..3949
                     /gene="MED13L"
                     /product="mediator of RNA polymerase II transcription
                     subunit 13-like"
                     /translation="DFLTISPFSLPFWERLLLDPYGGHRDVAYIVVCPENEALLEGAKT
                     FFRDLSAVYEMCRLGQHKPICKVLRDGIMRVGKTVAQKLTDELVSEWFNQPWSGEENDN
                     HSRLKLYAQVCRHHLAPYLATLQLDSSLLIPPKYQTPPAAAQGQATPGNAGPLAPNGSA
                     APPAGSAFNPTSNSSSTNPAASSSASGSSVPPVSSSASAPGISQISTTSSSGFSGSVGG
                     QNPSTGGISADRTQGNIGCGGDTDPGQSSSQPSQDGQESVTERERIGIPTEPDSADSHA
                     HPPAVVIYMVDPFTYAAEEDSTSGNFWLLSLMRCYTEMLDNLPEHMRNSFILQIVPCQY
                     MLQTMKDEQVFYIQYLKSMAFSVYCQCRRPLPTQIHIKSLTGFGPAASIEMTLKNPERP
                     SPIQLYSPPFILAPIKDKQTELGETFGEASQKYNVLFVGYCLSHDQRWLLASCTDLHGE
                     LLETCVVNIALPNRSRRSKVSARKIGLQKLWEWCIGIVQMTSLPWRVVIGRLGRLGHGE
                     LKDWSILLGECSLQTISKKLKDVCRMCGISAADSPSILSACLVAMEPQGSFVVMPDAVT
                     MGSVFGRSTALNMQSSQLNTPQDASCTHILVFPTSSTIQVAPANYPNEDGFSPNNDDMF
                     VDLPFPDDMDNDIGILMTGNLHSSPNSSPVPSPGSPSGIGVGSHFQHSRSQGERLLSRE
                     APEELKQQPLALGYFVSTAKAENLPQWFWSSCPQAQNQCPLFLKASLHHHISVAQTDEL
                     LPARNSQRVPHPLDSKTTSDVLRFVLEQYNALSWLTCNPATQDRTSCLPVHFVVLTQLY
                     NAIMNIL"
                     /note="mediator complex subunit 13 like; thyroid hormone
                     receptor-associated protein 2; thyroid hormone
                     receptor-associated protein complex 240 kDa component-like"
                     /note="color: #993366"
                     /db_xref="{'GI': '44771211', 'CCDS': 'CCDS9177.1',
                     'GeneID': '23389', 'HGNC': 'HGNC:22962', 'MIM': '608771'}"
                     /gene_synonym="MRFACD; PROSIT240; THRAP2; TRAP240L"
                     /protein_id="NP_056150.1"
                     /codon_start=1
                     /transl_table=1
                     /label="MED13L"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggatttcct
     1501 caccatctcg ccattctcct tgccgttttg ggagaggctc ttgttggacc catatggggg
     1561 ccaccgtgat gttgcctata ttgtggtgtg tccagaaaat gaggccttgc tcgaaggagc
     1621 caaaactttc ttcagggact tgagtgctgt atacgagatg tgtaggcttg ggcagcacaa
     1681 gcccatctgc aaagtgctac gtgacgggat catgcgcgtg ggaaaaactg tggcacagaa
     1741 gctgacagat gagcttgtga gtgagtggtt taaccagcct tggagcggcg aggagaatga
     1801 caatcattcc agactcaaac tttatgcgca agtttgccgc catcacctag caccttattt
     1861 agccactctg cagcttgata gcagcctatt gataccacct aaataccaga ccccaccagc
     1921 agcagcacag ggacaagcta cgccagggaa tgctgggccc ttagctccaa atggatcagc
     1981 agctccccca gctggcagtg catttaatcc cacctcgaat agtagttcta caaatcctgc
     2041 agcaagtagt tctgcatctg gttcctctgt gccaccggtc tcatcgtctg cctctgctcc
     2101 tggtattagc cagataagca ctacctcttc ttcaggattc agtggtagtg ttggagggca
     2161 gaaccccagc actgggggca tttctgcgga tagaacgcaa gggaacatag gctgtggtgg
     2221 agacactgac cctgggcaga gctcttctca gccctcacag gatggacaag agagtgttac
     2281 agaaagggag agaataggaa ttcccacgga gcctgactct gcagacagcc atgcccaccc
     2341 tccagctgtt gtcatttaca tggtggaccc gttcacgtat gctgcagagg aggactccac
     2401 ttctgggaac ttttggctgt tgagcttgat gcgctgctac acagaaatgc tggataattt
     2461 acctgagcat atgagaaatt ctttcattct ccagattgtg ccttgccagt acatgctgca
     2521 gacaatgaag gatgagcaag ttttctacat tcaatacttg aagtccatgg cattttcagt
     2581 gtactgtcag tgcaggcgac cactgcctac acagatccac attaaatccc tcacgggatt
     2641 tgggcctgca gccagcattg agatgaccct caagaaccct gagcggccca gcccaatcca
     2701 gctttactcc cctcccttta tattggcccc aatcaaagac aagcagacag agctgggaga
     2761 gacgtttggt gaggcgagcc agaaatacaa tgtgctcttc gtgggctatt gtctgtctca
     2821 cgaccagcgc tggcttttgg cttcctgcac tgacctccat ggggaattat tagagacctg
     2881 cgttgtaaat attgctttac caaacaggtc acggaggagt aaagtatctg cacgtaaaat
     2941 tggactacag aagttatggg agtggtgcat agggattgtc caaatgacat ctctaccctg
     3001 gagagttgta atcgggcgac ttgggcgtct tggccatggg gagcttaaag attggagtat
     3061 cctccttgga gaatgttcac tacagacaat cagcaaaaag ctcaaggatg tgtgccggat
     3121 gtgtggaatc tctgccgcag actctccttc tatccttagt gcctgcctgg ttgccatgga
     3181 gccccagggg tcctttgtag tgatgccaga tgctgtcaca atgggctctg tttttggccg
     3241 aagtactgca ctgaacatgc agtcatctca gctcaacacc cctcaagatg cttcttgtac
     3301 acacatcttg gtgttcccaa catcatcaac catccaggtg gctccagcca actaccccaa
     3361 tgaagatggg tttagcccca acaatgatga tatgtttgtt gaccttccat tcccagatga
     3421 tatggacaat gatattggca tattaatgac tgggaacctc cattcctctc ccaactcttc
     3481 cccagtaccc tccccaggct ctccttctgg aattggtgtg ggctctcact tccagcatag
     3541 tcggagccag ggtgagcgtc ttctttctag agaagcacca gaggagctaa agcagcagcc
     3601 cctggccctt gggtattttg tatcaactgc caaagctgag aatcttcccc agtggttttg
     3661 gtcatcgtgt ccccaggctc aaaaccagtg ccctctcttc ttaaaggctt cgctgcatca
     3721 ccacatttca gtagcacaga cagacgaact tctgcctgcc aggaattctc agcgggttcc
     3781 acaccctctt gactccaaaa ccacgtcgga tgttttaagg tttgttttgg agcagtacaa
     3841 cgctctgtcc tggctcacgt gcaatccggc cacccaggac cgtacttcct gccttcccgt
     3901 ccactttgtg gtgctcactc agttgtacaa tgccatcatg aatatacttt gtacagacta
     3961 caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa
     4021 gtaagaattc tgatgactcg agataatcaa cctctggatt acaaaatttg tgaaagattg
     4081 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     4141 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     4201 ttagttcttg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     4261 cggctgttgg gcactgacaa ttccgtggtg tttatttgtg aaatttgtga tgctattgct
     4321 ttatttgtaa ccatctagct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     4381 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     4441 tcagggggag atgtgggagg ttttttaaag atctggagac cggtgtggca cgtgcggacc
     4501 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     4561 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     4621 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
     4681 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
     4741 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     4801 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4861 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4921 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     4981 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     5041 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga
     5101 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     5161 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
     5221 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     5281 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     5341 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     5401 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     5461 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5521 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5581 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5641 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5701 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5761 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5821 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5881 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5941 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6001 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6061 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6121 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6181 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6241 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6301 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6361 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6421 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6481 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6541 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6601 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6661 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6721 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     6781 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6841 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6901 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6961 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7021 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7081 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7141 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7201 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
