LOCUS       .                       7411 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             415..4551
                     /translation="VNENTRMYIPEENHQGSNYGSPRPAHANMNANAAAGLAPEHIPTP
                     GAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALL
                     CLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEY
                     LFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANA
                     LGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIY
                     AIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKH
                     GITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLV
                     LGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEE
                     KPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCR
                     AAVKSNVFYWLVIFLVFLNTLTIASEHYNQPNWLTEVQDTANKALLALFTAEMLLKMYS
                     LGLQAYFVSLFNRFDCFVVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNS
                     LSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQS
                     LLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVD
                     NLADAESLTSAQKEEEEEKERKKLARTASPEKKQELVEKPAVGESKEEKIELKSITADG
                     ESPPATKINMDDLQPNENEDKSPYPNPETTGEEDEEEPEMPVGPRPRPLSELHLKEKAV
                     PMPEASAFFIFSSNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNH
                     ILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSA
                     INVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQ
                     LFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMAL
                     FTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIV
                     TFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMF
                     VLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMILKLIAFKPKHYFCDAWNT
                     FDALIVVGSIVDIAITEVNPAEHTQCSPSMNAEENSRISITFFRLFRVMRLVKLLSRGE
                     GIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQ"
                     /note="isoform 18 is encoded by transcript variant 18;
                     calcium channel, cardic dihydropyridine-sensitive, alpha-1
                     subunit; DHPR, alpha-1 subunit; voltage-dependent L-type
                     Ca2+ channel alpha 1 subunit; calcium channel,
                     voltage-dependent, L type, alpha 1C subunit; CACNA1C
                     intronic transcript 2; CACNA1C intronic transcript 2
                     (non-protein coding)"
                     /note="color: #993366"
                     /db_xref="{'GI': '120433602', 'CCDS': 'CCDS44794.1',
                     'GeneID': '775', 'HGNC': 'HGNC:1390', 'MIM': '114205'}"
                     /gene="CACNA1C"
                     /product="voltage-dependent L-type calcium channel subunit
                     alpha-1C isoform 18"
                     /gene_synonym="CACH2; CACN2; CACNA1C-IT2; CACNL1A1; CaV1.2;
                     CCHL1A1; LQT8; NEDHLSS; TS; TS. LQT8"
                     /transl_table=1
                     /codon_start=1
                     /protein_id="NP_000710.5"
                     /label="CACNA1C"
     CDS             5731..6591
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /gene="bla"
                     /product="β-lactamase"
                     /transl_table=1
                     /codon_start=1
                     /label="AmpR"
     rep_origin      6762..7350
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     rep_origin      4889..5344
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     promoter        156..367
                     /note="core promoter for human elongation factor EF-1α"
                     /note="color: #ffffff"
                     /label="EF-1α core promoter"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   4674..4814
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     promoter        5626..5730
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          4552..4592
                     /label="Splicing Donor"
                     /note="color: noColor"
     misc_feature    4596..4629
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     CDS             382..408
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /codon_start=1
                     /label="HA"
                     /note="color: #cc99b2"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgggca gagcgcacat cgcccacagt
      181 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg
      241 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
      301 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
      361 aacacagcgt acgccaccat gtacccttat gacgtacctg actatgctgg atctgtcaat
      421 gagaatacga ggatgtacat tccagaggaa aaccaccaag gttccaacta tgggagccca
      481 cgccccgccc atgccaacat gaatgccaat gcggcagcgg ggctggcccc tgagcacatc
      541 cccaccccgg gggctgccct gtcgtggcag gcggccatcg acgcagcccg gcaggctaag
      601 ctgatgggca gcgctggcaa tgcgaccatc tccacagtca gctccacgca gcggaagcgg
      661 cagcaatatg ggaaacccaa gaagcagggc agcaccacgg ccacacgccc gccccgagcc
      721 ctgctctgcc tgaccctgaa gaaccccatc cggagggcct gcatcagcat tgtcgaatgg
      781 aaaccatttg aaataattat tttactgact atttttgcca attgtgtggc cttagcgatc
      841 tatattccct ttccagaaga tgattccaac gccaccaatt ccaacctgga acgagtggaa
      901 tatctctttc tcataatttt tacggtggaa gcgtttttaa aagtaatcgc ctatggactc
      961 ctctttcacc ccaatgccta cctccgcaac ggctggaacc tactagattt tataattgtg
     1021 gttgtggggc tttttagtgc aattttagaa caagcaacca aagcagatgg ggcaaacgct
     1081 ctcggaggga aaggggccgg atttgatgtg aaggcgctga gggccttccg cgtgctgcgc
     1141 cccctgcggc tggtgtccgg agtcccaagt ctccaggtgg tcctgaattc catcatcaag
     1201 gccatggtcc ccctgctgca catcgccctg cttgtgctgt ttgtcatcat catctacgcc
     1261 atcatcggct tggagctctt catggggaag atgcacaaga cctgctacaa ccaggagggc
     1321 atagcagatg ttccagcaga agatgaccct tccccttgtg cgctggaaac gggccacggg
     1381 cggcagtgcc agaacggcac ggtgtgcaag cccggctggg atggtcccaa gcacggcatc
     1441 accaactttg acaactttgc cttcgccatg ctcacggtgt tccagtgcat caccatggag
     1501 ggctggacgg acgtgctgta ctgggtcaat gatgccgtag gaagggactg gccctggatc
     1561 tattttgtta cactaatcat catagggtca ttttttgtac ttaacttggt tctcggtgtg
     1621 cttagcggag agttttccaa agagagggag aaggccaagg cccggggaga tttccagaag
     1681 ctgcgggaga agcagcagct agaagaggat ctcaaaggct acctggattg gatcactcag
     1741 gccgaagaca tcgatcctga gaatgaggac gaaggcatgg atgaggagaa gccccgaaac
     1801 atgagcatgc ccaccagtga gaccgagtcc gtcaacaccg aaaacgtggc tggaggtgac
     1861 atcgagggag aaaactgcgg ggccaggctg gcccaccgga tctccaagtc aaagttcagc
     1921 cgctactggc gccggtggaa tcggttctgc agaaggaagt gccgcgccgc agtcaagtct
     1981 aatgtcttct actggctggt gattttcctg gtgttcctca acacgctcac cattgcctct
     2041 gagcactaca accagcccaa ctggctcaca gaagtccaag acacggcaaa caaggccctg
     2101 ctggccctgt tcacggcaga gatgctcctg aagatgtaca gcctgggcct gcaggcctac
     2161 ttcgtgtccc tcttcaaccg ctttgactgc ttcgtcgtgt gtggcggcat cctggagacc
     2221 atcctggtgg agaccaagat catgtcccca ctgggcatct ccgtgctcag atgcgtccgg
     2281 ctgctgagga ttttcaagat cacgaggtac tggaactcct tgagcaacct ggtggcatcc
     2341 ttgctgaact ctgtgcgctc catcgcctcc ctgctccttc tcctcttcct cttcatcatc
     2401 atcttctccc tcctggggat gcagctcttt ggaggaaagt tcaactttga tgagatgcag
     2461 acccggagga gcacattcga taacttcccc cagtccctcc tcactgtgtt tcagatcctg
     2521 accggggagg actggaattc ggtgatgtat gatgggatca tggcttatgg cggcccctct
     2581 tttccaggga tgttagtctg tatttacttc atcatcctct tcatctgtgg aaactatatc
     2641 ctactgaatg tgttcttggc cattgctgtg gacaacctgg ctgatgctga gagcctcaca
     2701 tctgcccaaa aggaggagga agaggagaag gagagaaaga agctggccag gactgccagc
     2761 ccagagaaga aacaagagtt ggtggagaag ccggcagtgg gggaatccaa ggaggagaag
     2821 attgagctga aatccatcac ggctgacgga gagtctccac ccgccaccaa gatcaacatg
     2881 gatgacctcc agcccaatga aaatgaggat aagagcccct accccaaccc agaaactaca
     2941 ggagaagagg atgaggagga gccagagatg cctgtcggcc ctcgcccacg accactctct
     3001 gagcttcacc ttaaggaaaa ggcagtgccc atgccagaag ccagcgcgtt tttcatcttc
     3061 agctctaaca acaggtttcg cctccagtgc caccgcattg tcaatgacac gatcttcacc
     3121 aacctgatcc tcttcttcat tctgctcagc agcatttccc tggctgctga ggacccggtc
     3181 cagcacacct ccttcaggaa ccatattctg ttttattttg atattgtttt taccaccatt
     3241 ttcaccattg aaattgctct gaagatgact gcttatgggg ctttcttgca caagggttct
     3301 ttctgccgga actacttcaa catcctggac ctgctggtgg tcagcgtgtc cctcatctcc
     3361 tttggcatcc agtccagtgc aatcaatgtc gtgaagatct tgcgagtcct gcgagtactc
     3421 aggcccctga gggccatcaa cagggccaag gggctaaagc atgtggttca gtgtgtgttt
     3481 gtcgccatcc ggaccatcgg gaacatcgtg attgtcacca ccctgctgca gttcatgttt
     3541 gcctgcatcg gggtccagct cttcaaggga aagctgtaca cctgttcaga cagttccaag
     3601 cagacagagg cggaatgcaa gggcaactac atcacgtaca aagacgggga ggttgaccac
     3661 cccatcatcc aaccccgcag ctgggagaac agcaagtttg actttgacaa tgttctggca
     3721 gccatgatgg ccctcttcac cgtctccacc ttcgaagggt ggccagagct gctgtaccgc
     3781 tccatcgact cccacacgga agacaagggc cccatctaca actaccgtgt ggagatctcc
     3841 atcttcttca tcatctacat catcatcatc gccttcttca tgatgaacat cttcgtgggc
     3901 ttcgtcatcg tcacctttca ggagcagggg gagcaggagt acaagaactg tgagctggac
     3961 aagaaccagc gacagtgcgt ggaatacgcc ctcaaggccc ggcccctgcg gaggtacatc
     4021 cccaagaacc agcaccagta caaagtgtgg tacgtggtca actccaccta cttcgagtac
     4081 ctgatgttcg tcctcatcct gctcaacacc atctgcctgg ccatgcagca ctacggccag
     4141 agctgcctgt tcaaaatcgc catgaacatc ctcaacatgc tcttcactgg cctcttcacc
     4201 gtggagatga tcctgaagct cattgccttc aaacccaagc actatttctg tgatgcatgg
     4261 aatacatttg acgccttgat tgttgtgggt agcattgttg atatagcaat caccgaggta
     4321 aacccagctg aacataccca atgctctccc tctatgaacg cagaggaaaa ctcccgcatc
     4381 tccatcacct tcttccgcct gttccgggtc atgcgtctgg tgaagctgct gagccgtggg
     4441 gagggcatcc ggacgctgct gtggaccttc atcaagtcct tccaggccct gccctatgtg
     4501 gccctcctga tcgtgatgct gttcttcatc tacgcggtga tcgggatgca ggtaagtatc
     4561 aaggttacaa gacaggttta aggagaccaa taagctatta ttcgtatagg atactttata
     4621 cgaagttatt aagttatgga gaccggtgtg gcacgtgcgg accgagcggc cgcaggaacc
     4681 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     4741 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     4801 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     4861 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     4921 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4981 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5041 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5101 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5161 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5221 cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5281 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     5341 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     5401 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     5461 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     5521 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     5581 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5641 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5701 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5761 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5821 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5881 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5941 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     6001 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6061 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6121 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6181 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6241 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6301 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6361 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6421 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6481 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6541 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6601 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6661 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6721 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6781 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6841 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6901 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6961 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7021 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7081 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7141 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7201 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7261 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7321 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7381 ctggcctttt gctggccttt tgctcacatg t
//
