LOCUS       .                       7053 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1493..3766
                     /protein_id="NP_000710.5"
                     /note="isoform 18 is encoded by transcript variant 18;
                     calcium channel, cardic dihydropyridine-sensitive, alpha-1
                     subunit; DHPR, alpha-1 subunit; voltage-dependent L-type
                     Ca2+ channel alpha 1 subunit; calcium channel,
                     voltage-dependent, L type, alpha 1C subunit; CACNA1C
                     intronic transcript 2; CACNA1C intronic transcript 2
                     (non-protein coding)"
                     /note="color: #993366"
                     /translation="VFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLAC
                     MPGKKCAPESEPSNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTR
                     DWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRL
                     VSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLD
                     QVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDI
                     GPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVSYYQSDGRSAFPQ
                     TFTTQRPLHINKAGSSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRLP
                     RPAGYPSTVSTVEGHGPPLSPAIRVQEVAWKLSSNRCHSRESQAAMAGQEETSQDETYE
                     VKMNHDTEACSEPSLLSTEMLSYQDDENRQLTLPEEDKRDIRQSPKRGFLRSASLGRRA
                     SFHLECLKRQKDRGGDISQKTVLPLHLVHHQALAVAGLSPLLQRSHSPASFPRPFATPP
                     ATPGSRGWPPQPVPTLRLEGVESSEKLNSSFPSIHCGSWAETTPGGGGSSAARRVRPVS
                     LMVPSQAGAPGRQFHGSASSLVEAVLISEGLGQFAQDPKFIEVTTQELADACDMTIEEM
                     ESAADNILSGGAPQSPNGALLPFVNCRDAGQDRAGGEEDAGCVRARGRPSEEELQDSRV
                     YVSSL"
                     /gene="CACNA1C"
                     /db_xref="{'GI': '120433602', 'CCDS': 'CCDS44794.1',
                     'GeneID': '775', 'HGNC': 'HGNC:1390', 'MIM': '114205'}"
                     /product="voltage-dependent L-type calcium channel subunit
                     alpha-1C isoform 18"
                     /codon_start=1
                     /gene_synonym="CACH2; CACN2; CACNA1C-IT2; CACNL1A1; CaV1.2;
                     CCHL1A1; LQT8; NEDHLSS; TS; TS. LQT8"
                     /transl_table=1
                     /label="CACNA1C"
     CDS             288..1318
                     /protein_id=""
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /product="site-specific recombinase"
                     /codon_start=1
                     /transl_table=1
                     /label="Cre"
     CDS             5373..6233
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /product="β-lactamase"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     rep_origin      6404..6992
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     rep_origin      4531..4986
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     terminator      3842..4273
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   4316..4456
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     promoter        5268..5372
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     CDS             3773..3838
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /codon_start=1
                     /transl_table=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     protein_bind    1367..1400
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /bound_moiety="Cre recombinase"
                     /label="lox2272-JTZ17"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtgtttgg
     1501 gaaaattgcc ctgaatgata ccacagagat caaccggaac aacaactttc agaccttccc
     1561 ccaggccgtg ctgctcctct tcaggtgtgc caccggggag gcctggcagg acatcatgct
     1621 ggcctgcatg ccaggcaaga agtgtgcccc agagtccgag cccagcaaca gcacggaggg
     1681 tgaaacaccc tgtggtagca gctttgctgt cttctacttc atcagcttct acatgctctg
     1741 tgccttcctg atcatcaacc tctttgtagc tgtcatcatg gacaactttg actacctgac
     1801 aagggactgg tccatccttg gtccccacca cctggatgag tttaaaagaa tctgggcaga
     1861 gtatgaccct gaagccaagg gtcgtatcaa acacctggat gtggtgaccc tcctccggcg
     1921 gattcagccg ccactaggtt ttgggaagct gtgccctcac cgcgtggctt gcaaacgcct
     1981 ggtctccatg aacatgcctc tgaacagcga cgggacagtc atgttcaatg ccaccctgtt
     2041 tgccctggtc aggacggccc tgaggatcaa aacagaaggg aacctagaac aagccaatga
     2101 ggagctgcgg gcgatcatca agaagatctg gaagcggacc agcatgaagc tgctggacca
     2161 ggtggtgccc cctgcaggtg atgatgaggt caccgttggc aagttctacg ccacgttcct
     2221 gatccaggag tacttccgga agttcaagaa gcgcaaagag cagggccttg tgggcaagcc
     2281 ctcccagagg aacgcgctgt ctctgcaggc tggcttgcgc acactgcatg acatcgggcc
     2341 tgagatccga cgggccatct ctggagatct caccgctgag gaggagctgg acaaggccat
     2401 gaaggaggct gtgtccgctg cttctgaaga tgacatcttc aggagggccg gtggcctgtt
     2461 cggcaaccac gtcagctact accaaagcga cggccggagc gccttccccc agaccttcac
     2521 cactcagcgc ccgctgcaca tcaacaaggc gggcagcagc cagggcgaca ctgagtcgcc
     2581 atcccacgag aagctggtgg actccacctt caccccgagc agctactcgt ccaccggctc
     2641 caacgccaac atcaacaacg ccaacaacac cgccctgggt cgcctccctc gccccgccgg
     2701 ctaccccagc acagtcagca ctgtggaggg ccacgggccc cccttgtccc ctgccatccg
     2761 ggtgcaggag gtggcgtgga agctcagctc caacaggtgc cactcccggg agagccaggc
     2821 agccatggcg ggtcaggagg agacgtctca ggatgagacc tatgaagtga agatgaacca
     2881 tgacacggag gcctgcagtg agcccagcct gctctccaca gagatgctct cctaccagga
     2941 tgacgaaaat cggcaactga cgctcccaga ggaggacaag agggacatcc ggcaatctcc
     3001 gaagaggggt ttcctccgct ctgcctcact aggtcgaagg gcctccttcc acctggaatg
     3061 tctgaagcga cagaaggacc gagggggaga catctctcag aagacagtcc tgcccttgca
     3121 tctggttcat catcaggcat tggcagtggc aggcctgagc cccctcctcc agagaagcca
     3181 ttcccctgcc tcattcccta ggccttttgc caccccacca gccacacctg gcagccgagg
     3241 ctggccccca cagcccgtcc ccaccctgcg gcttgagggg gtcgagtcca gtgagaaact
     3301 caacagcagc ttcccatcca tccactgcgg ctcctgggct gagaccaccc ccggtggcgg
     3361 gggcagcagc gccgcccgga gagtccggcc cgtctccctc atggtgccca gccaggctgg
     3421 ggccccaggg aggcagttcc acggcagtgc cagcagcctg gtggaagcgg tcttgatttc
     3481 agaaggactg gggcagtttg ctcaagatcc caagttcatc gaggtcacca cccaggagct
     3541 ggccgacgcc tgcgacatga ccatagagga gatggagagc gcggccgaca acatcctcag
     3601 cgggggcgcc ccacagagcc ccaatggcgc cctcttaccc tttgtgaact gcagggacgc
     3661 ggggcaggac cgagccgggg gcgaagagga cgcgggctgt gtgcgcgcgc ggggtcgacc
     3721 gagtgaggag gagctccagg acagcagggt ctacgtcagc agcctgtgta cagactacaa
     3781 agaccatgac ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta
     3841 actcgagata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     3901 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3961 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttagt tcttgccacg
     4021 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4081 gacaattccg tggtgtttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatc
     4141 tagctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     4201 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
     4261 ggaggttttt taaagatctg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa
     4321 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     4381 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     4441 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     4501 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
     4561 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4621 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4681 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4741 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4801 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4861 accctatctc gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4921 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     4981 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     5041 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     5101 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     5161 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     5221 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5281 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5341 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     5401 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     5461 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5521 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     5581 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5641 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5701 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5761 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5821 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5881 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     5941 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6001 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6061 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6121 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6181 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6241 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6301 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6361 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6421 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6481 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6541 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6601 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6661 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6721 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6781 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6841 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6901 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6961 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7021 tcctggcctt ttgctggcct tttgctcaca tgt
//
