LOCUS       .                       6177 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    3362..3395
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     intron          3318..3358
                     /label="Splicing Donor"
                     /note="color: noColor"
     promoter        4392..4496
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   3440..3580
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     rep_origin      3655..4110
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      5528..6116
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     CDS             4497..5357
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /product="β-lactamase"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     CDS             724..3317
                     /translation="MGRVGYWTLLVLPALLVWRGPAPSAAAEKGPPALNIAVMLGHSHD
                     VTERELRTLWGPEQAAGLPLDVNVVALLMNRTDPKSLITHVCDLMSGARIHGLVFGDDT
                     DQEAVAQMLDFISSHTFVPILGIHGGASMIMADKDPTSTFFQFGASIQQQATVMLKIMQ
                     DYDWHVFSLVTTIFPGYREFISFVKTTVDNSFVGWDMQNVITLDTSFEDAKTQVQLKKI
                     HSSVILLYCSKDEAVLILSEARSLGLTGYDFFWIVPSLVSGNTELIPKEFPSGLISVSY
                     DDWDYSLEARVRDGIGILTTAASSMLEKFSYIPEAKASCYGQMERPEVPMHTLHPFMVN
                     VTWDGKDLSFTEEGYQVHPRLVVIVLNKDREWEKVGKWENHTLSLRHAVWPRYKSFSDC
                     EPDDNHLSIVTLEEAPFVIVEDIDPLTETCVRNTVPCRKFVKINNSTNEGMNVKKCCKG
                     FCIDILKKLSRTVKFTYDLYLVTNGKHGKKVNNVWNGMIGEVVYQRAVMAVGSLTINEE
                     RSEVVDFSVPFVETGISVMVSRSNGTVSPSAFLEPFSASVWVMMFVMLLIVSAIAVFVF
                     EYFSPVGYNRNLAKGKAPHGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWA
                     FFAVIFLASYTANLAAFMIQEEFVDQVTGLSDKKFQRPHDYSPPFRFGTVPNGSTERNI
                     RNNYPYMHQYMTKFNQKGVEDALVSLKTGKLDAFIYDAAVLNYKAGRDEGCKLVTIGSG
                     YIFATTGYGIALQKGSPWKRQIDLALLQFVGDGEMEELETLWLTGICHNEKNEVMSSQL
                     DIDNMAGVFYMLAAAMALSLITFIWEHLFYWKLRFCFTGVCSDRPGLLFSIS"
                     /codon_start=1
                     /transl_table=1
                     /label="GRIN2A"
                     /note="color: #993366"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgggca gagtgggcta ttggaccctg ctggtgctgc cggcccttct ggtctggcgc
      781 ggtccggcgc cgagcgcggc ggcggagaag ggtccccccg cgctaaatat tgcggtgatg
      841 ctgggtcaca gccacgacgt gacagagcgc gaacttcgaa cactgtgggg ccccgagcag
      901 gcggcggggc tgcccctgga cgtgaacgtg gtagctctgc tgatgaaccg caccgacccc
      961 aagagcctca tcacgcacgt gtgcgacctc atgtccgggg cacgcatcca cggcctcgtg
     1021 tttggggacg acacggacca ggaggccgta gcccagatgc tggattttat ctcctcccac
     1081 accttcgtcc ccatcttggg cattcatggg ggcgcatcta tgatcatggc tgacaaggat
     1141 ccgacgtcta ccttcttcca gtttggagcg tccatccagc agcaagccac ggtcatgctg
     1201 aagatcatgc aggattatga ctggcatgtc ttctccctgg tgaccactat cttccctggc
     1261 tacagggaat tcatcagctt cgtcaagacc acagtggaca acagctttgt gggctgggac
     1321 atgcagaatg tgatcacact ggacacttcc tttgaggatg caaagacaca agtccagctg
     1381 aagaagatcc actcttctgt catcttgctc tactgttcca aagacgaggc tgttctcatt
     1441 ctgagtgagg cccgctccct tggcctcacc gggtatgatt tcttctggat tgtccccagc
     1501 ttggtctctg ggaacacgga gctcatccca aaagagtttc catcgggact catttctgtc
     1561 tcctacgatg actgggacta cagcctggag gcgagagtga gggacggcat tggcatccta
     1621 accaccgctg catcttctat gctggagaag ttctcctaca tccccgaggc caaggccagc
     1681 tgctacgggc agatggagag gccagaggtc ccgatgcaca ccttgcaccc atttatggtc
     1741 aatgttacat gggatggcaa agacttatcc ttcactgagg aaggctacca ggtgcacccc
     1801 aggctggtgg tgattgtgct gaacaaagac cgggaatggg aaaaggtggg caagtgggag
     1861 aaccatacgc tgagcctgag gcacgccgtg tggcccaggt acaagtcctt ctccgactgt
     1921 gagccggatg acaaccatct cagcatcgtc accctggagg aggccccatt cgtcatcgtg
     1981 gaagacatag accccctgac cgagacgtgt gtgaggaaca ccgtgccatg tcggaagttc
     2041 gtcaaaatca acaattcaac caatgagggg atgaatgtga agaaatgctg caaggggttc
     2101 tgcattgata ttctgaagaa gctttccaga actgtgaagt ttacttacga cctctatctg
     2161 gtgaccaatg ggaagcatgg caagaaagtt aacaatgtgt ggaatggaat gatcggtgaa
     2221 gtggtctatc aacgggcagt catggcagtt ggctcgctca ccatcaatga ggaacgttct
     2281 gaagtggtgg acttctctgt gccctttgtg gaaacgggaa tcagtgtcat ggtttcaaga
     2341 agtaatggca ccgtctcacc ttctgctttt ctagaaccat tcagcgcctc tgtctgggtg
     2401 atgatgtttg tgatgctgct cattgtttct gccatagctg tttttgtctt tgaatacttc
     2461 agccctgttg gatacaacag aaacttagcc aaagggaaag caccccatgg gccttctttt
     2521 acaattggaa aagctatatg gcttctttgg ggcctggtgt tcaataactc cgtgcctgtc
     2581 cagaatccta aagggaccac cagcaagatc atggtatctg tatgggcctt cttcgctgtc
     2641 atattcctgg ctagctacac agccaatctg gctgccttca tgatccaaga ggaatttgtg
     2701 gaccaagtga ccggcctcag tgacaaaaag tttcagagac ctcatgacta ttccccacct
     2761 tttcgatttg ggacagtgcc taatggaagc acggagagaa acattcggaa taactatccc
     2821 tacatgcatc agtacatgac caaatttaat cagaaaggag tagaggacgc cttggtcagc
     2881 ctgaaaacgg ggaagctgga cgctttcatc tacgatgccg cagtcttgaa ttacaaggct
     2941 gggagggatg aaggctgcaa gctggtgacc atcgggagtg ggtacatctt tgccaccacc
     3001 ggttatggaa ttgcccttca gaaaggctct ccttggaaga ggcagatcga cctggccttg
     3061 cttcagtttg tgggtgatgg tgagatggag gagctggaga ccctgtggct cactgggatc
     3121 tgccacaacg agaagaacga ggtgatgagc agccagctgg acattgacaa catggcgggc
     3181 gtattctaca tgctggctgc cgccatggcc cttagcctca tcaccttcat ctgggagcac
     3241 ctcttctact ggaagctgcg cttctgtttc acgggcgtgt gctccgaccg gcctgggttg
     3301 ctcttctcca tcagcaggta agtatcaagg ttacaagaca ggtttaagga gaccaataag
     3361 ctattattcg tataggatac tttatacgaa gttattaagt tatggagacc ggtgtggcac
     3421 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     3481 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     3541 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
     3601 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
     3661 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3721 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3781 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     3841 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     3901 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     3961 tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga
     4021 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4081 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     4141 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     4201 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     4261 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     4321 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     4381 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4441 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4501 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4561 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     4621 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     4681 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     4741 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     4801 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     4861 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     4921 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     4981 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5041 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5101 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5161 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5221 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5281 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5341 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5401 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5461 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5521 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5581 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5641 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     5701 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5761 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5821 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5881 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5941 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6001 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6061 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6121 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
