LOCUS       .                       6577 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    1367..1400
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /bound_moiety="Cre recombinase"
                     /label="lox2272-JTZ17"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     CDS             288..1318
                     /protein_id=""
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /product="site-specific recombinase"
                     /codon_start=1
                     /transl_table=1
                     /label="Cre"
     CDS             3297..3362
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /codon_start=1
                     /transl_table=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     promoter        4792..4896
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   3840..3980
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     terminator      3366..3797
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     rep_origin      4055..4510
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     rep_origin      5928..6516
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     CDS             4897..5757
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /product="β-lactamase"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     CDS             1493..3290
                     /protein_id="NP_001127879.1"
                     /note="isoform 1 precursor is encoded by transcript variant
                     1; N-methyl-D-aspartate receptor channel, subunit
                     epsilon-1; N-methyl-D-aspartate receptor subunit 2A;
                     N-methyl D-aspartate receptor subtype 2A; glutamate
                     receptor, ionotropic, N-methyl D-aspartate 2A; glutamate
                     receptor ionotropic, NMDA 2A; glutamate ionotropic receptor
                     NMDA 2A"
                     /note="color: #993366"
                     /translation="GIYSCIHGVHIEEKKKSPDFNLTGSQSNMLKLLRSAKNISSMSNM
                     NSSRMDSPKRAADFIQRGSLIMDMVSDKGNLMYSDNRSFQGKESIFGDNMNELQTFVAN
                     RQKDNLNNYVFQGQHPLTLNESNPNTVEVAVSTESKANSRPRQLWKKSVDSIRQDSLSQ
                     NPVSQRDEATAENRTHSLKSPRYLPEEMAHSDISETSNRATCHREPDNSKNHKTKDNFK
                     RSVASKYPKDCSEVERTYLKTKSSSPRDKIYTIDGEKEPGFHLDPPQFVENVTLPENVD
                     FPDPYQDPSENFRKGDSTLPMNRNPLHNEEGLSNNDQYKLYSKHFTLKDKGSPHSETSE
                     RYRQNSTHCRSCLSNMPTYSGHFTMRSPFKCDACLRMGNLYDIDEDQMLQETGNPATGE
                     QVYQQDWAQNNALQLQKNKLRISRQHSYDNIVDKPRELDLSRPSRSISLKDRERLLEGN
                     FYGSLFSVPSSKLSGKKSSLFPQGLEDSKRSKSLLPDHTSDNPFLHSHRDDQRLVIGRC
                     PSDPYKHSLPSQAVNDSYLRSSLRSTASYCSRDSRGHNDVYISEHVMPYAANKNNMYST
                     PRVLNSCSNRRVYKKMPSIESDV"
                     /gene="GRIN2A"
                     /db_xref="{'GI': '197313636', 'CCDS': 'CCDS10539.1',
                     'GeneID': '2903', 'HGNC': 'HGNC:4585', 'MIM': '138253'}"
                     /product="glutamate receptor ionotropic, NMDA 2A isoform 1
                     precursor"
                     /codon_start=1
                     /gene_synonym="EPND; FESD; GluN2A; LKS; NMDAR2A; NR2A"
                     /transl_table=1
                     /label="GRIN2A"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggggcatct
     1501 acagctgcat tcatggagtg cacattgaag aaaagaagaa gtctccagac ttcaatctga
     1561 cgggatccca gagcaacatg ttaaaactcc tccggtcagc caaaaacatt tccagcatgt
     1621 ccaacatgaa ctcctcaaga atggactcac ccaaaagagc tgctgacttc atccaaagag
     1681 gttccctcat catggacatg gtttcagata aggggaattt gatgtactca gacaacaggt
     1741 cctttcaggg gaaagagagc atttttggag acaacatgaa cgaactccaa acatttgtgg
     1801 ccaaccggca gaaggataac ctcaataact atgtattcca gggacaacat cctcttactc
     1861 tcaatgagtc caaccctaac acggtggagg tggccgtgag cacagaatcc aaagcgaact
     1921 ctagaccccg gcagctgtgg aagaaatccg tggattccat acgccaggat tcactatccc
     1981 agaatccagt ctcccagagg gatgaggcaa cagcagagaa taggacccac tccctaaaga
     2041 gccctaggta tcttccagaa gagatggccc actctgacat ttcagaaacg tcaaatcggg
     2101 ccacgtgcca cagggaacct gacaacagta agaaccacaa aaccaaggac aactttaaaa
     2161 ggtcagtggc ctccaaatac cccaaggact gtagtgaggt cgagcgcacc tacctgaaaa
     2221 ccaaatcaag ctcccctaga gacaagatct acactataga tggtgagaag gagcctggtt
     2281 tccacttaga tccaccccag tttgttgaaa atgtgaccct gcccgagaac gtggacttcc
     2341 cggaccccta ccaggatccc agtgaaaact tccgcaaggg ggactccacg ctgccaatga
     2401 accggaaccc cttgcataat gaagaggggc tttccaacaa cgaccagtat aaactctact
     2461 ccaagcactt caccttgaaa gacaagggtt ccccgcacag tgagaccagc gagcgatacc
     2521 ggcagaactc cacgcactgc agaagctgcc tttccaacat gcccacctat tcaggccact
     2581 tcaccatgag gtcccccttc aagtgcgatg cctgcctgcg gatggggaac ctctatgaca
     2641 tcgatgaaga ccagatgctt caggagacag gtaacccagc caccggggag caggtctacc
     2701 agcaggactg ggcacagaac aatgcccttc aattacaaaa gaacaagcta aggattagcc
     2761 gtcagcattc ctacgataac attgtcgaca aacctaggga gctagacctt agcaggccct
     2821 cccggagcat aagcctcaag gacagggaac ggcttctgga gggaaatttt tacggcagcc
     2881 tgtttagtgt cccctcaagc aaactctcgg ggaaaaaaag ctcccttttc ccccaaggtc
     2941 tggaggacag caagaggagc aagtctctct tgccagacca cacctccgat aaccctttcc
     3001 tccactccca cagggatgac caacgcttgg ttattgggag atgcccctcg gacccttaca
     3061 aacactcgtt gccatcccag gcggtgaatg acagctatct tcggtcgtcc ttgaggtcaa
     3121 cggcatcgta ctgttccagg gacagtcggg gccacaatga tgtgtatatt tcggagcatg
     3181 ttatgcctta tgctgcaaat aagaataata tgtactctac ccccagggtt ttaaattcct
     3241 gcagcaatag acgcgtgtac aagaaaatgc ctagtatcga atctgatgtt tgtacagact
     3301 acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca
     3361 agtaactcga gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     3421 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     3481 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tagttcttgc
     3541 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     3601 cactgacaat tccgtggtgt ttatttgtga aatttgtgat gctattgctt tatttgtaac
     3661 catctagctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     3721 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga
     3781 tgtgggaggt tttttaaaga tctggagacc ggtgtggcac gtgcggaccg agcggccgca
     3841 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
     3901 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
     3961 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     4021 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     4081 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4141 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4201 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4261 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4321 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4381 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4441 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     4501 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     4561 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     4621 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4681 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     4741 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4801 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4861 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     4921 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     4981 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5041 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5101 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5161 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5221 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5281 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5341 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5401 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5461 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5521 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5581 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5641 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5701 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5761 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5821 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     5881 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5941 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6001 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6061 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6121 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6181 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6241 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6301 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6361 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6421 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6481 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6541 cggttcctgg ccttttgctg gccttttgct cacatgt
//
