LOCUS       .                       5658 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    2843..2876
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     intron          2799..2839
                     /label="Splicing Donor"
                     /note="color: noColor"
     promoter        3873..3977
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   2921..3061
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     rep_origin      3136..3591
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      5009..5597
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     CDS             3978..4838
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /product="β-lactamase"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     CDS             724..2798
                     /translation="MGTALLQRGGCFLLCLSLLLLGCWAELGSGLEFPGAEGQWTRFPK
                     WNACCESEMSFQLKTRSARGLVLYFDDEGFCDFLELILTRGGRLQLSFSIFCAEPATLL
                     ADTPVNDGAWHSVRIRRQFRNTTLFIDQVEAKWVEVKSKRRDMTVFSGLFVGGLPPELR
                     AAALKLTLASVREREPFKGWIRDVRVNSSQVLPVDSGEVKLDDEPPNSGGGSPCEAGEE
                     GEGGVCLNGGVCSVVDDQAVCDCSRTGFRGKDCSQEDNNVEGLAHLMMGDQGKSKGKEE
                     YIATFKGSEYFCYDLSQNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAV
                     SLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVDGILTTTG
                     YTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKQ
                     GDPKMKIHGVVAFKCENVATLDPITFETPESFISLPKWNAKKTGSISFDFRTTEPNGLI
                     LFSHGKPRHQKDAKHPQMIKVDFFAIEMLDGHLYLLLDMGSGTIKIKALLKKVNDGEWY
                     HVDFQRDGRSGTISVNTLRTPYTAPGESEILDLDDELYLGGLPENKAGLVFPTEVWTAL
                     LNYGYVGCIRDLFIDGQSKDIRQMAEVQSTAGVKPSCSKETAKPCLSNPCKNNGMC"
                     /codon_start=1
                     /transl_table=1
                     /label="NRXN1"
                     /note="color: #993366"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgggga cggcgctgct ccagcgcggg ggctgttttc ttctgtgcct ctcgctgctg
      781 ctcctgggct gctgggcgga gctgggcagc gggctggagt ttccgggcgc cgagggccaa
      841 tggacgcgct tccccaagtg gaacgcctgc tgcgagagcg agatgagctt ccagctcaag
      901 actcgcagcg cccgcggcct cgtgctctac ttcgacgacg agggcttctg cgacttcctg
      961 gagctgattc tgacgcgcgg cggccgcctg cagctcagct tctccatctt ctgcgctgag
     1021 cctgcgacgc tcctggccga cacgccggtt aacgacggcg cctggcacag cgtgcgcatc
     1081 cgccgccagt tccgcaacac cacgctcttc atcgaccagg tggaggccaa gtgggtggag
     1141 gtcaagtcca agcgcaggga catgacggtg ttcagcggcc ttttcgtcgg ggggctgccc
     1201 ccggaactgc gcgccgcggc gctcaagctc accctggcct cggtgaggga gcgggagccc
     1261 ttcaaggggt ggattcgtga cgtgagggtc aactcctcgc aggtcctgcc cgtggacagc
     1321 ggcgaggtga agctggacga tgagccgccc aacagcggcg ggggaagccc gtgcgaggcg
     1381 ggcgaggagg gcgagggcgg ggtgtgcctc aacggaggtg tgtgctccgt ggtggacgac
     1441 caggccgtgt gcgactgctc gcgaaccggc ttccgcggca aggactgcag ccaagaagac
     1501 aacaatgtgg aaggtctggc gcacctgatg atgggcgacc aaggtaaaag taaaggaaaa
     1561 gaagaatata ttgccacgtt caaaggatct gaatacttct gctacgactt gtctcaaaac
     1621 cccattcaaa gcagcagtga tgaaataact ctgtcattta aaacccttca gaggaatgga
     1681 ctgatgcttc acactgggaa atcggctgat tatgtcaatc ttgccctgaa aaatggagct
     1741 gtctctctgg tcattaattt gggatcaggg gcctttgaag cactagtgga gcctgtgaat
     1801 ggaaagttta atgataatgc ctggcatgat gtgaaagtca ccaggaatct gcgtcagcac
     1861 tcaggcattg gacacgctat ggtgacaata tcagtggatg ggattcttac cacaacgggc
     1921 tacacgcaag aagattatac catgctgggg tctgatgact ttttctatgt tggaggcagt
     1981 cccagcacag ccgaccttcc agggtcacca gtcagtaaca actttatggg ctgtctcaaa
     2041 gaggttgtat ataaaaataa tgatgtgagg ctggaattat ctcgacttgc caagcaagga
     2101 gatcctaaga tgaagatcca tggagtggtg gcatttaaat gtgagaatgt tgcaacttta
     2161 gacccaatca cctttgaaac cccagagtct ttcatctctt tgcctaaatg gaatgcaaag
     2221 aaaactggct ccatatcatt tgatttccgt acaacagagc caaatggcct catcttattt
     2281 agccatggca agccaagaca tcagaaagat gccaagcacc cacagatgat aaaggtggac
     2341 ttctttgcta ttgagatgct agatggccac ctctacctcc tcctggacat ggggtcaggt
     2401 actataaaaa taaaagccct gttgaagaaa gtgaatgatg gagaatggta tcatgtggac
     2461 ttccagagag acggacggtc aggtaccatt tctgtcaaca cgttgcgtac tccctacact
     2521 gctcctggtg agagtgagat tctggacctg gatgatgagt tgtacctggg ggggctgcca
     2581 gaaaataaag ctggccttgt cttccccacc gaggtgtgga ctgctctgct caactatggc
     2641 tacgtgggct gcatcaggga tttgttcatc gatggccaaa gcaaagatat ccggcaaatg
     2701 gctgaagttc aaagtactgc tggagtgaag ccttcctgct caaaggaaac agcaaaaccg
     2761 tgccttagca acccttgcaa aaacaatggc atgtgcaggt aagtatcaag gttacaagac
     2821 aggtttaagg agaccaataa gctattattc gtataggata ctttatacga agttattaag
     2881 ttatggagac cggtgtggca cgtgcggacc gagcggccgc aggaacccct agtgatggag
     2941 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
     3001 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
     3061 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     3121 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     3181 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     3241 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     3301 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     3361 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     3421 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct
     3481 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     3541 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     3601 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     3661 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     3721 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     3781 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     3841 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3901 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     3961 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     4021 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     4081 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     4141 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     4201 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     4261 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     4321 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     4381 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     4441 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     4501 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     4561 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     4621 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     4681 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     4741 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     4801 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     4861 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     4921 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4981 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5041 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5101 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     5161 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5221 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5281 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5341 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5401 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5461 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5521 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5581 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5641 ggccttttgc tcacatgt
//
