LOCUS       .                       7225 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    1367..1400
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /bound_moiety="Cre recombinase"
                     /label="lox2272-JTZ17"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     CDS             288..1318
                     /protein_id=""
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /product="site-specific recombinase"
                     /codon_start=1
                     /transl_table=1
                     /label="Cre"
     CDS             3945..4010
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /codon_start=1
                     /transl_table=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     promoter        5440..5544
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   4488..4628
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     terminator      4014..4445
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     rep_origin      4703..5158
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     rep_origin      6576..7164
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     CDS             5545..6405
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /product="β-lactamase"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     CDS             1493..3938
                     /protein_id="NP_001317007.1"
                     /note="isoform alpha4 precursor is encoded by transcript
                     variant alpha4; neurexin I"
                     /note="color: #993366"
                     /translation="DGWNRYVCDCSGTGYLGRSCEREATVLSYDGSMFMKIQLPVVMHT
                     EAEDVSLRFRSQRAYGILMATTSRDSADTLRLELDAGRVKLTVNLDCIRINCNSSKGPE
                     TLFAGYNLNDNEWHTVRVVRRGKSLKLTVDDQQAMTGQMAGDHTRLEFHNIETGIITER
                     RYLSSVPSNFIGHLQSLTFNGMAYIDLCKNGDIDYCELNARFGFRNIIADPVTFKTKSS
                     YVALATLQAYTSMHLFFQFKTTSLDGLILYNSGDGNDFIVVELVKGYLHYVFDLGNGAN
                     LIKGSSNKPLNDNQWHNVMISRDTSNLHTVKIDTKITTQITAGARNLDLKSDLYIGGVA
                     KETYKSLPKLVHAKEGFQGCLASVDLNGRLPDLISDALFCNGQIERGCEGPSTTCQEDS
                     CSNQGVCLQQWDGFSCDCSMTSFSGPLCNDPGTTYIFSKGGGQITYKWPPNDRPSTRAD
                     RLAIGFSTVQKEAVLVRVDSSSGLGDYLELHIHQGKIGVKFNVGTDDIAIEESNAIIND
                     GKYHVVRFTRSGGNATLQVDSWPVIERYPAGNNDNERLAIARQRIPYRLGRVVDEWLLD
                     KGRQLTIFNSQATIIIGGKEQGQPFQGQLSGLYYNGLKVLNMAAENDANIAIVGNVRLV
                     GEVPSSMTTESTATAMQSEMSTSIMETTTTLATSTARRGKPPTKEPISQTTDDILVASA
                     ECPSDDEDIDPCEPSSGGLANPTRAGGREPYPGSAEVIRESSSTTGMVVGIVAAAALCI
                     LILLYAMYKYRNRDEGSYHVDESRNYISNSAQSNGAVVKEKQPSSAKSSNKNKKNKDKE
                     YYV"
                     /gene="NRXN1"
                     /db_xref="{'GI': '1052292350', 'CCDS': 'CCDS82451.1',
                     'GeneID': '9378', 'HGNC': 'HGNC:8008', 'MIM': '600565'}"
                     /product="neurexin-1 isoform alpha4 precursor"
                     /codon_start=1
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /transl_table=1
                     /label="NRXN1"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac agggatgggt
     1501 ggaacagata tgtctgtgat tgttccggaa caggctatct tggcaggtcc tgtgagagag
     1561 aggcaacggt tttgagctat gatgggagca tgtttatgaa aattcagctc cccgtagtca
     1621 tgcatacgga ggctgaggat gtttccttac ggttccgatc ccagcgtgca tatggcattc
     1681 tgatggcaac cacttctaga gactctgctg acaccctccg cctggagcta gacgcaggac
     1741 gtgtgaaact gacggtcaat ctagattgta tcaggattaa ctgtaattcc agcaaaggtc
     1801 ccgagactct ttttgctggc tataacctca atgataacga gtggcacaca gtgcgtgtag
     1861 ttcggcgtgg aaaaagttta aagttaacag tggatgacca acaggccatg acaggtcaaa
     1921 tggcaggtga tcatactagg ctggagttcc ataacataga gactggcatc atcacagaac
     1981 gacggtatct ttcttctgtc ccctccaact tcattggaca cctgcagagc ttgacattta
     2041 atggaatggc atacattgac ctgtgtaaaa atggcgacat agattactgt gagcttaatg
     2101 ccagatttgg cttcaggaac atcatagcag atcctgtcac cttcaagacc aaatcgagct
     2161 atgttgcctt agctaccttg caagcctaca cttctatgca tctttttttc cagttcaaga
     2221 caacatccct agatggatta attctatata acagtgggga tggaaatgac tttattgtgg
     2281 ttgaattagt taaagggtac ttacattacg tgtttgattt gggaaatggt gctaacctca
     2341 tcaaaggaag ctcaaataaa cctctcaatg acaatcagtg gcacaacgtg atgatatcaa
     2401 gggacaccag caacctccac actgtaaaga ttgacacaaa aatcacaacg caaatcaccg
     2461 ccggagccag gaacttagac ctcaagagtg acttatatat aggaggagta gctaaagaaa
     2521 catacaaatc cttaccaaaa cttgtacatg ccaaagaagg ctttcaaggc tgcctggcat
     2581 cagttgattt aaatggacgg cttccggacc tcatctccga tgctcttttc tgcaacggac
     2641 agatcgagag aggatgtgaa gggcccagca caacctgcca agaggactca tgttccaatc
     2701 aaggtgtgtg cttgcaacaa tgggatggct tcagctgtga ctgtagtatg acttccttca
     2761 gtggaccact ctgcaatgac cctgggacga catatatctt tagcaaaggt ggtggacaaa
     2821 tcacgtataa gtggcctcct aatgaccgac ccagtacacg agcagacaga ctggccatag
     2881 gttttagcac tgttcagaaa gaagccgtat tggtgcgagt ggacagttct tcaggcttgg
     2941 gtgactacct agaactgcat atacaccagg gaaaaattgg agttaagttt aatgttggga
     3001 cagatgacat cgccattgaa gaatccaatg caatcattaa tgatgggaaa taccatgtag
     3061 ttcgtttcac gaggagtggt ggcaatgcca cgttgcaggt ggacagctgg ccagtgatcg
     3121 agcgctaccc tgcaggaaac aatgataacg agcgcctggc gattgctaga cagcgaattc
     3181 catatcgact tggtcgagta gttgatgaat ggctactcga caaagggcgt cagctcacaa
     3241 tcttcaatag ccaagcaacc ataataattg gcgggaaaga gcagggccag cccttccagg
     3301 gccagctctc tgggctgtac tacaatggct tgaaagttct gaatatggca gccgaaaacg
     3361 atgccaacat cgccatagtg ggaaatgtga gactggttgg tgaagtgcct tcctctatga
     3421 caactgagtc aacagccact gccatgcaat cagagatgtc cacatcaatt atggagacta
     3481 ccacgaccct ggctactagc acagccagaa gaggaaagcc cccgacaaaa gaacccatta
     3541 gccagaccac agatgacatc cttgtggcct cagcagagtg tcccagcgat gatgaggaca
     3601 ttgacccctg tgagccgagc tcaggtgggt tagccaaccc aacccgagca ggcggcagag
     3661 agccgtatcc aggctcagca gaagtgatcc gggagtccag cagcaccacg ggtatggtcg
     3721 ttgggatagt agccgctgcc gccctgtgca tccttatcct cctctatgcc atgtacaagt
     3781 acagaaaccg ggatgaaggc tcataccatg tggacgagag tcgaaactac atcagtaact
     3841 cagcacagtc caatggggct gttgtaaagg agaaacaacc cagcagtgcg aaaagctcca
     3901 acaaaaataa gaaaaacaag gataaagagt attatgtctg tacagactac aaagaccatg
     3961 acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag taactcgaga
     4021 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     4081 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     4141 tatggctttc attttctcct ccttgtataa atcctggtta gttcttgcca cggcggaact
     4201 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     4261 cgtggtgttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca tctagcttta
     4321 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     4381 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt
     4441 tttaaagatc tggagaccgg tgtggcacgt gcggaccgag cggccgcagg aacccctagt
     4501 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
     4561 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
     4621 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     4681 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4741 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4801 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4861 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     4921 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4981 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5041 tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5101 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
     5161 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     5221 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     5281 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     5341 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     5401 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5461 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5521 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5581 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5641 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5701 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5761 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     5821 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5881 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5941 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     6001 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6061 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     6121 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6181 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6241 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6301 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6361 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6421 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6481 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6541 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6601 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6661 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6721 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6781 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6841 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6901 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6961 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7021 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7081 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7141 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7201 ttttgctggc cttttgctca catgt
//
