LOCUS       .                       6622 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    1367..1400
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /label="lox2272-JTZ17"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     CDS             3330..3395
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /codon_start=1
                     /transl_table=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     promoter        4837..4941
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     repeat_region   3885..4025
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     terminator      3411..3842
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     rep_origin      4100..4555
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     rep_origin      5973..6561
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     CDS             4942..5802
                     /gene="bla"
                     /product="β-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     CDS             288..1318
                     /product="site-specific recombinase"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /protein_id=""
                     /codon_start=1
                     /transl_table=1
                     /label="Cre"
     CDS             1493..3323
                     /gene="ZMYM2"
                     /product="zinc finger MYM-type protein 2 isoform 1"
                     /translation="CDCCKSQGTLKERVQWRGEMKHFCDQHCLLRFYCQQNEPNMTTQK
                     GPENLHYDQGCQTSRTKMTGSAPPPSPTPNKEMKNKAVLCKPLTMTKATYCKPHMQTKS
                     CQTDDTWRTEYVPVPIPVPVYIPVPMHMYSQNIPVPTTVPVPVPVPVFLPAPLDSSEKI
                     PAAIEELKSKVSSDALDTELLTMTDMMSEDEGKTETTNINSVIIETDIIGSDLLKNSDP
                     ETQSSMPDVPYEPDLDIEIDFPRAAEELDMENEFLLPPVFGEEYEEQPRPRSKKKGAKR
                     KAVSGYQSHDDSSDNSECSFPFKYTYGVNAWKHWVKTRQLDEDLLVLDELKSSKSVKLK
                     EDLLSHTTAELNYGLAHFVNEIRRPNGENYAPDSIYYLCLGIQEYLCGSNRKDNIFIDP
                     GYQTFEQELNKILRSWQPSILPDGSIFSRVEEDYLWRIKQLGSHSPVALLNTLFYFNTK
                     YFGLKTVEQHLRLSFGTVFRHWKKNPLTMENKACLRYQVSSLCGTDNEDKITTGKRKHE
                     DDEPVFEQIENTANPSRCPVKMFECYLSKSPQNLNQRMDVFYLQPECSSSTDSPVWYTS
                     TSLDRNTLENMLVRVLLVKDIYDKDNYELDEDTD"
                     /note="isoform 1 is encoded by transcript variant 2; zinc
                     finger protein 198; rearranged in an atypical
                     myeloproliferative disorder; fused in myeloproliferative
                     disorders protein; zinc finger, MYM-type 2"
                     /note="color: #993366"
                     /db_xref="{'GI': '37574606', 'CCDS': 'CCDS45016.1',
                     'GeneID': '7750', 'HGNC': 'HGNC:12989', 'MIM': '602221'}"
                     /gene_synonym="FIM; MYM; NECRC; RAMP; SCLL; ZNF198"
                     /protein_id="NP_932072.1"
                     /codon_start=1
                     /transl_table=1
                     /label="ZMYM2"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtgtgact
     1501 gttgtaaatc tcaaggaact cttaaagagc gagttcagtg gcgtggggaa atgaaacatt
     1561 tctgtgatca acattgctta ctgcgtttct actgtcaaca aaatgagccc aacatgacaa
     1621 ctcagaaagg acctgaaaac ttacattatg atcagggttg tcagacatct cgaaccaaaa
     1681 tgacaggttc agcaccaccc ccttctccaa cacctaacaa agagatgaag aacaaagcag
     1741 ttctttgcaa acctttaaca atgacaaaag ctacttactg taaacctcac atgcagacca
     1801 aatcttgtca gacagatgat acttggagga cagaatatgt tccagtgcct atccctgtgc
     1861 ctgtgtatat cccagttcct atgcacatgt acagtcagaa tattcctgtt cctactacag
     1921 ttcctgttcc tgtgccagtt cctgtttttc tgcctgctcc attggacagc agtgagaaga
     1981 ttcctgcagc aattgaggag ctaaaaagca aggtttcttc agatgctctt gatacagagt
     2041 tgcttacaat gacggatatg atgagtgaag acgaggggaa aacagagaca accaacatca
     2101 acagtgtaat tattgaaaca gatataattg gttcagacct tttgaagaac tctgacccag
     2161 agacacagtc cagcatgcct gatgtaccat atgaaccaga tttggatatc gaaatagatt
     2221 ttcccagagc tgctgaggag cttgatatgg aaaatgaatt tttattacca cctgtttttg
     2281 gcgaagaata tgaggaacag cccagacctc gatctaaaaa aaagggagcc aagagaaagg
     2341 ctgtatcagg ataccagtct catgatgata gttctgacaa ttcagaatgc agctttcctt
     2401 tcaaatatac gtatggcgta aatgcatgga aacactgggt caaaactagg caacttgatg
     2461 aagatcttct ggtattagat gagttaaaat cttctaaatc agtaaagtta aaagaggatc
     2521 tactctctca caccacagct gagcttaact atgggttagc tcattttgtc aatgagatcc
     2581 gacggccaaa tggagagaat tatgcacctg acagcatcta ttacctttgc cttggaatac
     2641 aggagtattt gtgtggaagt aatcgaaaag acaacatatt tattgatcct ggataccaaa
     2701 catttgagca agaattgaat aaaatactgc gaagctggca accaagcata cttccagatg
     2761 ggtcaatatt ctctcgagtt gaagaagact atctctggag gataaaacaa ctaggatcac
     2821 actctccagt agctcttctg aatacactgt tctactttaa cactaagtat tttggcctga
     2881 aaacagtgga acaacactta agactttcct ttggcactgt gtttaggcat tggaaaaaaa
     2941 atcctttaac gatggaaaac aaagcgtgtc ttcgatacca agtgtcttcc ttgtgtggaa
     3001 cagataatga agataaaatt actactggaa aaagaaaaca tgaagatgat gagccagtat
     3061 ttgaacaaat tgaaaacaca gccaatcctt ccagatgtcc tgtgaaaatg tttgaatgct
     3121 acttgtctaa aagtccacag aatcttaatc agaggatgga tgttttttat ttgcaaccag
     3181 aatgctctag ttctacagat agccctgtct ggtatacgtc tacttcactg gaccgaaaca
     3241 ccttggaaaa tatgcttgta cgggttcttc tagtaaaaga tatttatgat aaagacaatt
     3301 atgaactgga tgaagacaca gactgtacag actacaaaga ccatgacggt gattataaag
     3361 atcatgacat cgactacaag gatgacgatg acaagtaaga attctgatga ctcgagataa
     3421 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     3481 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     3541 ggctttcatt ttctcctcct tgtataaatc ctggttagtt cttgccacgg cggaactcat
     3601 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     3661 ggtgtttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatct agctttattt
     3721 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     3781 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt
     3841 aaagatctgg agaccggtgt ggcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat
     3901 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt
     3961 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct
     4021 gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     4081 acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4141 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4201 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4261 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     4321 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     4381 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     4441 ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     4501 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg
     4561 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     4621 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     4681 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     4741 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     4801 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4861 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4921 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4981 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5041 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5101 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     5161 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     5221 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     5281 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     5341 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5401 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5461 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     5521 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5581 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5641 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5701 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5761 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5821 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5881 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5941 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6001 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6061 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     6121 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6181 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6241 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6301 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6361 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6421 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6481 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6541 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6601 tgctggcctt ttgctcacat gt
//
