LOCUS       .                       6079 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             727..753
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /codon_start=1
                     /label="HA"
                     /note="color: #cc99b2"
     misc_feature    3264..3297
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     intron          3220..3260
                     /label="Splicing Donor"
                     /note="color: noColor"
     promoter        4294..4398
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   3342..3482
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     rep_origin      3557..4012
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      5430..6018
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             4399..5259
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /product="β-lactamase"
                     /gene="bla"
                     /transl_table=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /codon_start=1
                     /label="AmpR"
     CDS             760..3219
                     /translation="TTKRSLFVRLVPCRCLRGEEETVTTLDYSHCSLEQVPKEIFTFEK
                     TLEELYLDANQIEELPKQLFNCQSLHKLSLPDNDLTTLPASIANLINLRELDVSKNGIQ
                     EFPENIKNCKVLTIVEASVNPISKLPDGFSQLLNLTQLYLNDAFLEFLPANFGRLTKLQ
                     ILELRENQLKMLPKTMNRLTQLERLDLGSNEFTEVPEVLEQLSGLKEFWMDANRLTFIP
                     GFIGSLKQLTYLDVSKNNIEMVEEGISTCENLQDLLLSSNSLQQLPETIGSLKNITTLK
                     IDENQLMYLPDSIGGLISVEELDCSFNEVEALPSSIGQLTNLRTFAADHNYLQQLPPEI
                     GSWKNITVLFLHSNKLETLPEEMGDMQKLKVINLSDNRLKNLPFSFTKLQQLTAMWLSD
                     NQSKPLIPLQKETDSETQKMVLTNYMFPQQPRTEDVMFISDNESFNPSLWEEQRKQRAQ
                     VAFECDEDKDEREAPPREGNLKRYPTPYPDELKNMVKTVQTIVHRLKDEETNEDSGRDL
                     KPHEDQQDINKDVGVKTSESTTTVKSKVDEREKYMIGNSVQKISEPEAEISPGSLPVTA
                     NMKASENLKHIVNHDDVFEESEELSSDEEMKMAEMRPPLIETSINQPKVVALSNNKKDD
                     TKETDSLSDEVTHNSNQNNSNCSSPSRMSDSVSLNTDSSQDTSLCSPVKQTHIDINSKI
                     RQEDENFNSLLQNGDILNSSTEEKFKAHDKKDFNLPEYDLNVEERLVLIEKSVDSTATA
                     DDTHKLDHINMNLNKLITNDTFQPEIMERSKTQDIVLGTSFLSINSKEETEHLENGNKY
                     PNLESVNK"
                     /product="erbin isoform 1"
                     /gene="ERBIN"
                     /transl_table=1
                     /note="isoform 1 is encoded by transcript variant 1;
                     densin-180-like protein; protein LAP2; epididymis secretory
                     protein Li 78"
                     /note="color: #993366"
                     /protein_id="NP_001240626.1"
                     /db_xref="{'GI': '358679311', 'CCDS': 'CCDS58953.1',
                     'GeneID': '55914', 'HGNC': 'HGNC:15842', 'MIM': '606944'}"
                     /gene_synonym="ERBB2IP; HEL-S-78; LAP2"
                     /codon_start=1
                     /label="ERBIN"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatcta ctacaaaacg aagtttgttt
      781 gtgcggttgg taccatgtcg ctgtctacga ggggaagagg agactgtcac tactcttgat
      841 tattctcatt gcagcttaga acaagttccg aaagagattt ttacttttga aaaaaccttg
      901 gaggaactct atttagatgc taatcagatt gaagagcttc caaagcaact ttttaactgt
      961 cagtctttac acaaactgag tttgccagac aatgatttaa caacgttacc agcatccatt
     1021 gcaaacctta ttaatctcag ggaactggat gtcagcaaga atggaataca ggagtttcca
     1081 gaaaatataa aaaattgtaa agttttgaca attgtggagg ccagtgtaaa ccctatttcc
     1141 aagctccctg atggattttc tcagctgtta aacctaaccc agttgtatct gaatgatgct
     1201 tttcttgagt tcttgccagc aaattttggc agattaacta aactccaaat attagagctt
     1261 agagaaaacc agttaaaaat gttgcctaaa actatgaata gactgaccca gctggaaaga
     1321 ttggatttgg gaagtaacga attcacggaa gtgcctgaag tacttgagca actaagtgga
     1381 ttgaaagagt tttggatgga tgctaataga ctgactttta ttccagggtt tattggtagt
     1441 ttgaaacagc tcacatattt ggatgtttct aaaaataata ttgaaatggt tgaagaagga
     1501 atttcaacat gtgaaaacct tcaagacctc ctattatcaa gcaattcact tcagcagctt
     1561 cctgagacta ttggttcgtt gaagaatata acaacgctta aaatagatga aaaccagtta
     1621 atgtatctgc cagactctat aggagggtta atatcagtag aagaactgga ttgtagtttc
     1681 aatgaagttg aagctttgcc ttcatctatt gggcagctta ctaacttaag aacttttgct
     1741 gctgatcata attacttaca gcagttgccc ccagagattg gaagctggaa aaatataact
     1801 gtgctgtttc tccattccaa taaacttgag acacttccag aggaaatggg tgatatgcaa
     1861 aaattaaaag tcattaattt aagtgataat agattaaaga atttaccctt tagctttaca
     1921 aagctacagc aattgacagc tatgtggctc tcagataatc agtccaaacc cctgatacct
     1981 cttcaaaaag aaactgattc agagacccag aaaatggtgc ttaccaacta catgttccct
     2041 caacagccaa ggactgagga tgttatgttt atatcagata atgaaagttt taacccttca
     2101 ttgtgggagg aacagaggaa acagcgggct caagttgcat ttgaatgtga tgaagacaaa
     2161 gatgaaaggg aggcacctcc cagggaggga aatttaaaaa gatatccaac accataccca
     2221 gatgagctta agaatatggt caaaactgtt caaaccattg tacatagatt aaaagatgaa
     2281 gagaccaatg aagactcagg aagagatttg aaaccacatg aagatcaaca agatataaat
     2341 aaagatgtgg gtgtgaagac ctcagaaagt actactacag taaaaagcaa agttgatgaa
     2401 agagaaaaat atatgatagg aaactctgta cagaagatca gtgaacctga agctgagatt
     2461 agtcctggga gtttaccagt gactgcaaat atgaaagcct ctgagaactt gaagcatatt
     2521 gttaaccatg atgatgtttt tgaggaatct gaagaacttt cttctgatga agagatgaaa
     2581 atggcggaga tgcgaccacc attaattgaa acctctatta accagccaaa agtcgtagca
     2641 cttagtaata acaaaaaaga tgatacaaag gaaacagatt ctttatcaga tgaagttaca
     2701 cacaatagca atcagaataa cagcaattgt tcttctccat ctcggatgtc tgattcagtt
     2761 tctcttaata ctgatagtag tcaagacacc tcactctgct ctccagtgaa acaaactcat
     2821 attgatatta attccaaaat caggcaagaa gatgaaaatt ttaacagcct tttacaaaat
     2881 ggagatattt taaacagttc aacagaggaa aagttcaaag ctcatgataa aaaagatttt
     2941 aacttacctg aatatgattt gaatgttgaa gagcgattag ttctaattga gaaaagtgtt
     3001 gactcaacag ccacagctga tgacactcac aaattagatc atatcaatat gaatcttaat
     3061 aaacttataa ctaatgatac atttcaacca gagatcatgg aaagatcaaa aacacaggat
     3121 attgtgcttg gaacaagctt tttaagcatt aattctaaag aggaaactga gcacttggaa
     3181 aatggaaaca agtatcctaa tttggaatcc gtaaataagg taagtatcaa ggttacaaga
     3241 caggtttaag gagaccaata agctattatt cgtataggat actttatacg aagttattaa
     3301 gttatggaga ccggtgtggc acgtgcggac cgagcggccg caggaacccc tagtgatgga
     3361 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc
     3421 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca
     3481 ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     3541 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3601 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3661 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     3721 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
     3781 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     3841 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc
     3901 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     3961 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
     4021 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     4081 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     4141 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     4201 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     4261 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     4321 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     4381 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     4441 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     4501 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     4561 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     4621 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     4681 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     4741 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     4801 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     4861 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     4921 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     4981 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     5041 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     5101 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     5161 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     5221 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     5281 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     5341 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5401 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5461 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5521 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     5581 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5641 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5701 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5761 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5821 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5881 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5941 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6001 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6061 tggccttttg ctcacatgt
//
