LOCUS       .                       6552 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1493..3265
                     /translation="VNGHSEETSQSPNRTEPHDSDCSVDLGISKSTEDLSPQKSGPVGS
                     VVKSHSITNMEIGGLKIYDILSDNGPQQPSTTVKITSAVDGKNIVRSKSATLLYDQPLQ
                     VFTGSSSSSDLISGTKAIFKFDSNHNPEEPNIIRGPTSGPQSAPQIYGPPQYNIQYSSS
                     AAVKDTLWHSKQNPQIDHASFPPQLLPRSESTENQSYAKHSANMNFSNHNNVRANTAYH
                     LHQRLGPARHGEMWAISPNDRLIPAVTRSTIQRQSSVSSTASVNLGDPGSTRRAQIPEG
                     DYLSYREFHSAGRTPPMMPGSQRPLSARTYSIDGPNASRPQSARPSINEIPERTMSVSD
                     FNYSRTSPSKRPNARVGSEHSLLDPPGKSKVPRDWREQVLRHIEAKKLEKKHPQTSSSG
                     DPCQDGIFISGQQNYSSATLSHKDVPPDSLMKMPLSNGQMGQPLRPQANYSQIHHPPQA
                     SVARHPSREQLIDYLMLKVAHQPPYTQPHCSPRQGHELAKQEIRVRVEKDPELGFSISG
                     GVGGRGNPFRPDDDGIFVTRVQPEGPASKLLQPGDKIIQANGYSFINIEHGQAVSLLKT
                     FQNTVELIIVREVSS"
                     /note="isoform 1 is encoded by transcript variant 1;
                     densin-180-like protein; protein LAP2; epididymis secretory
                     protein Li 78"
                     /note="color: #993366"
                     /db_xref="{'GI': '358679311', 'CCDS': 'CCDS58953.1',
                     'GeneID': '55914', 'HGNC': 'HGNC:15842', 'MIM': '606944'}"
                     /gene="ERBIN"
                     /product="erbin isoform 1"
                     /gene_synonym="ERBB2IP; HEL-S-78; LAP2"
                     /transl_table=1
                     /codon_start=1
                     /protein_id="NP_001240626.1"
                     /label="ERBIN"
     CDS             288..1318
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /product="site-specific recombinase"
                     /transl_table=1
                     /codon_start=1
                     /protein_id=""
                     /label="Cre"
     CDS             4872..5732
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /gene="bla"
                     /product="β-lactamase"
                     /transl_table=1
                     /codon_start=1
                     /label="AmpR"
     rep_origin      5903..6491
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     rep_origin      4030..4485
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     terminator      3341..3772
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   3815..3955
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     promoter        4767..4871
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     CDS             3272..3337
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /codon_start=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     protein_bind    1367..1400
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /label="lox2272-JTZ17"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtaaatgg
     1501 acattctgag gaaacttccc agtctcctaa taggactgaa ccacatgaca gtgattgttc
     1561 tgttgactta ggtatttcca aaagcactga agatctctcc cctcagaaaa gtggtccagt
     1621 tggatctgtt gtgaaatctc atagcataac taatatggag attggagggc taaaaatcta
     1681 tgatattctt agtgataatg gacctcagca gccaagtaca accgttaaaa tcacatctgc
     1741 tgttgatgga aaaaatatag tcaggagcaa gtctgccaca ctgttgtatg atcaaccatt
     1801 gcaggtattt actggttctt cctcatcttc tgatttaata tcaggaacaa aggcaatttt
     1861 caagtttgat tcaaatcata atcccgaaga gccaaatata ataagaggcc ccacaagtgg
     1921 cccacaatct gcacctcaaa tatatggtcc tccacagtat aatatccaat acagtagcag
     1981 tgctgcagtc aaagacactt tgtggcactc caaacaaaat ccccaaatag accatgccag
     2041 ttttcctcct cagctccttc ctagatcaga gagcacagaa aatcaaagtt atgctaaaca
     2101 ttctgccaat atgaatttct ctaatcataa caatgttcga gctaatactg cataccattt
     2161 acatcagaga cttggcccag caagacatgg ggaaatgtgg gccatctcac caaacgaccg
     2221 acttattcct gcagtaactc gaagtacaat ccagcgacaa agtagtgtgt cctccacagc
     2281 ctctgtaaat cttggtgatc caggctctac aaggcgggct cagattcctg aaggagatta
     2341 tttatcatac agagagttcc actcagcggg aagaactcct ccaatgatgc caggatcaca
     2401 gagacccctt tctgcacgaa catacagcat agatggtcca aatgcatcaa gacctcagag
     2461 tgctcgaccc tctattaatg aaataccaga gagaactatg tcagttagtg atttcaatta
     2521 ttcacggact agtccttcaa aaagaccaaa tgcaagggtt ggttctgagc attctttatt
     2581 agatcctcca ggaaaaagta aagttcctcg tgactggaga gaacaagtac ttcgacatat
     2641 tgaagccaaa aagttagaaa agaagcatcc ccagacatcc agttcaggag atccttgtca
     2701 agatggtata ttcatttcag gacagcagaa ctactcatca gccacactta gtcacaaaga
     2761 tgttcctcca gacagcttga tgaaaatgcc tttgagtaat ggacagatgg gccagcctct
     2821 caggcctcag gcaaattata gtcaaataca tcacccccct caggcatctg tggcaaggca
     2881 tccctctaga gaacaactaa ttgattactt gatgctgaaa gtggcccacc agcctccata
     2941 tacacagccc cattgttctc ctagacaagg ccatgaactg gcaaaacaag agattcgagt
     3001 gagggttgaa aaggatccag aacttggatt tagcatatca ggtggtgtcg ggggtagagg
     3061 aaacccattc agacctgatg atgatggtat atttgtaaca agggtacaac ctgaaggacc
     3121 agcatcaaaa ttactgcagc caggtgataa aattattcag gctaatggct acagttttat
     3181 aaatattgaa catggacaag cagtgtcctt gctaaaaact ttccagaata cagttgaact
     3241 catcattgta cgagaagttt cctcatgtac agactacaaa gaccatgacg gtgattataa
     3301 agatcatgac atcgactaca aggatgacga tgacaagtaa ctcgagataa tcaacctctg
     3361 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3421 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3481 ttctcctcct tgtataaatc ctggttagtt cttgccacgg cggaactcat cgccgcctgc
     3541 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgtttatt
     3601 tgtgaaattt gtgatgctat tgctttattt gtaaccatct agctttattt gtgaaatttg
     3661 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     3721 ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagatctgg
     3781 agaccggtgt ggcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc
     3841 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
     3901 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc
     3961 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
     4021 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4081 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4141 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4201 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
     4261 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4321 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt
     4381 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     4441 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
     4501 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     4561 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     4621 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     4681 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     4741 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4801 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4861 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4921 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4981 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5041 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5101 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5161 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5221 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5281 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5341 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5401 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5461 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5521 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5581 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5641 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5701 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5761 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5821 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5881 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5941 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6001 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     6061 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6121 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6181 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6241 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6301 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6361 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6421 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6481 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6541 ttgctcacat gt
//
