LOCUS       .                       5306 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    2491..2524
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     intron          2447..2487
                     /label="Splicing Donor"
                     /note="color: noColor"
     promoter        3521..3625
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   2569..2709
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     rep_origin      2784..3239
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      4657..5245
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     CDS             3626..4486
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /product="β-lactamase"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     CDS             724..2446
                     /protein_id="NP_001351997.1"
                     /note="isoform g precursor is encoded by transcript variant
                     7"
                     /note="color: #993366"
                     /translation="MAAAGARLSPGPGSGLRGRPRLCFHPGPPPLLPLLLLFLLLLPPP
                     PLLAGATAAASREPDSPCRLKTVTVSTLPALRESDIGWSGARAGAGAGTGAGAAAAAAS
                     PGSPGSAGTAAESRLLLFVRNELPGRIAVQDDLDNTELPFFTLEMSGTAADISLVHWRQ
                     QWLENGTLYFHVSMSSSGQLAQATAPTLQEPSEIVEEQMHILHISVMGGLIALLLLLLV
                     FTVALYAQRRWQKRRRIPQKSASTEATHEIHYIPSVLLGPQARESFRSSRLQTHNSVIG
                     VPIRETPILDDYDCEEDEEPPRRANHVSREDEFGSQVTHTLDSLGHPGEEKVDFEKKAA
                     AEATQETVESLMQKFKESFRANTPIEIGQLQPPLRSTSAGKRKRRSKSRGGISFGRAKG
                     TSGSEADDETQLTFYTEQYRSRRRSKGLLKSPVNKTALTLIAVSSCILAMVCGSQMSCP
                     LTVKVTLHVPEHFIADGSSFVVSEGSYLDISDWLNPAKLSLYYQINATSPWVRDLCGQR
                     TTDACEQLCDPETGECSCHEGYAPDPVHRHLCVRSDWGQSEGPWPYTTLERGYDLVT"
                     /gene="ASTN2"
                     /db_xref="{'GI': '1433353889', 'CCDS': 'CCDS6816.1',
                     'GeneID': '23245', 'HGNC': 'HGNC:17021', 'MIM': '612856'}"
                     /product="astrotactin-2 isoform g precursor"
                     /codon_start=1
                     /gene_synonym="bA67K19.1"
                     /transl_table=1
                     /label="ASTN2"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatggccg ccgccggcgc ccggctcagc cccggccccg gctcggggct ccgggggcgg
      781 ccgaggctct gcttccaccc ggggccgccg ccactgctgc cgctgctgct gctgttcctg
      841 ctcctgctgc cgccgccgcc gctgctggcc ggcgccaccg ccgctgcctc gcgggagccc
      901 gacagcccgt gccggctgaa gaccgtcacg gtgtccacac tgcccgccct gcgggagagc
      961 gacatcggct ggagcggcgc ccgcgccggg gccggggctg ggaccggggc cggagccgcc
     1021 gccgccgccg cgtccccggg ctctcctggc tctgccggca ccgccgccga gtcgcgcctc
     1081 ctgctctttg tgcgtaacga gctgccgggg cgcatcgcgg tgcaggacga cctggacaac
     1141 accgagctgc ccttcttcac cctggagatg tctggcacag cagcggacat ctcgctggtg
     1201 cactggagac agcagtggct ggagaatggc accttgtact tccacgtctc catgagcagc
     1261 tccgggcagc tggcccaagc caccgccccc actctccagg agccctcgga gattgttgag
     1321 gagcagatgc acatcctcca catttctgtg atgggtggcc tcatcgcgct gctgctgctg
     1381 ctgctggtgt tcaccgtggc gctgtacgcc cagcgacgtt ggcagaagcg tcgccgcatc
     1441 ccccagaaga gcgcaagcac agaagccact catgagatcc actacatccc atctgtgctg
     1501 ctgggtcccc aggcgcggga gagcttccgt tcatcccggc tgcaaaccca caattccgtc
     1561 attggcgtgc ccatccggga gactcccatc ctggatgact atgactgtga ggaggatgag
     1621 gagccaccta ggcgggccaa ccatgtctcc cgcgaggacg agtttggcag ccaggtgacc
     1681 cacactctgg acagtctggg acatccaggg gaagagaagg tggactttga gaagaaagca
     1741 gcagctgagg cgactcagga gacagtggag tccctgatgc agaagttcaa ggagagtttc
     1801 cgcgctaaca cgcccatcga gatcggtcag ctgcaaccac ccctgcgcag cacatcggca
     1861 gggaagagga agcggaggag caagtctcga ggaggaatca gctttgggag agccaagggg
     1921 acgtcgggct cagaggcaga cgatgaaact cagctgacat tctacacgga gcagtaccgc
     1981 agtcgccgcc gcagcaaagg tttgctgaaa agcccagtga acaagacagc cctgacactg
     2041 attgctgtga gttcctgcat cctggccatg gtgtgtggca gccagatgtc ttgtccactc
     2101 actgtgaagg tgactctgca tgtgcccgag cacttcatag cagatggaag cagcttcgtg
     2161 gtgagtgaag ggagctacct ggacatctcc gactggttaa acccggccaa gctttccctg
     2221 tattaccaga tcaatgccac ctccccatgg gtgagggacc tctgtggaca aaggacgaca
     2281 gatgcctgtg agcagctctg cgacccagaa accggagagt gcagctgtca tgaaggctat
     2341 gcccctgacc ctgttcacag acacctgtgt gtgcgcagtg actggggaca gagtgaagga
     2401 ccttggccct acacgacact tgagaggggc tatgatctgg tgacaggtaa gtatcaaggt
     2461 tacaagacag gtttaaggag accaataagc tattattcgt ataggatact ttatacgaag
     2521 ttattaagtt atggagaccg gtgtggcacg tgcggaccga gcggccgcag gaacccctag
     2581 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
     2641 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
     2701 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     2761 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     2821 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     2881 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     2941 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
     3001 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     3061 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     3121 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     3181 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
     3241 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     3301 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     3361 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     3421 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     3481 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3541 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3601 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     3661 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     3721 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     3781 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     3841 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     3901 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     3961 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4021 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4081 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4141 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     4201 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     4261 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     4321 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     4381 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     4441 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     4501 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     4561 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     4621 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4681 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4741 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4801 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4861 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4921 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4981 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5041 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5101 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5161 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5221 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5281 cttttgctgg ccttttgctc acatgt
//
