LOCUS       .                       7103 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             3862..3888
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /codon_start=1
                     /transl_table=1
                     /label="HA"
                     /note="color: #cc99b2"
     CDS             3832..3858
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /codon_start=1
                     /transl_table=1
                     /label="HA"
                     /note="color: #cc99b2"
     CDS             3802..3828
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /codon_start=1
                     /transl_table=1
                     /label="HA"
                     /note="color: #cc99b2"
     protein_bind    1367..1400
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /bound_moiety="Cre recombinase"
                     /label="lox2272-JTZ17"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     promoter        5318..5422
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     repeat_region   4366..4506
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     terminator      3892..4323
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     rep_origin      4581..5036
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     rep_origin      6454..7042
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     CDS             5423..6283
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /product="β-lactamase"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     CDS             288..1318
                     /protein_id=""
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /product="site-specific recombinase"
                     /codon_start=1
                     /transl_table=1
                     /label="Cre"
     CDS             1493..3786
                     /protein_id="NP_001351997.1"
                     /note="isoform g precursor is encoded by transcript variant
                     7"
                     /note="color: #993366"
                     /translation="EQAPEKILRSTFSLGQGLWLPVSKSFVVPPVELSINPLASCKTDV
                     LVTEDPADVREEAMLSTYFETINDLLSSFGPVRDCSRNNGGCTRNFKCVSDRQVDSSGC
                     VCPEELKPMKDGSGCYDHSKGIDCSDGFNGGCEQLCLQQTLPLPYDATSSTIFMFCGCV
                     EEYKLAPDGKSCLMLSDVCEGPKCLKPDSKFNDTLFGEMLHGYNNRTQHVNQGQVFQMT
                     FRENNFIKDFPQLADGLLVIPLPVEEQCRGVLSEPLPDLQLLTGDIRYDEAMGYPMVQQ
                     WRVRSNLYRVKLSTITLAAGFTNVLKILTKESSREELLSFIQHYGSHYIAEALYGSELT
                     CIIHFPSKKVQQQLWLQYQKETTELGSKKELKSMPFITYLSGLLTAQMLSDDQLISGVE
                     IRCEEKGRCPSTCHLCRRPGKEQLSPTPVLLEINRVVPLYTLIQDNGTKEAFKSALMSS
                     YWCSGKGDVIDDWCRCDLSAFDANGLPNCSPLLQPVLRLSPTVEPSSTVVSLEWVDVQP
                     AIGTKVSDYILQHKKVDEYTDTDLYTGEFLSFADDLLSGLGTSCVAAGRSHGEVPEVSI
                     YSVIFKCLEPDGLYKFTLYAVDTRGRHSELSTVTLRTACPLVDDNKAEEIADKIYNLYN
                     GYTSGKEQQMAYNTLMEVSASMLFRVQHHYNSHYEKFGDFVWRSEDELGPRKAHLILRR
                     LERVSSHCSSLLRSAYIQSRVETVPYLFCRSEEVRPAGMVWYSILKDTKITCEEKMVSM
                     ARNTYGESKGR"
                     /gene="ASTN2"
                     /db_xref="{'GI': '1433353889', 'CCDS': 'CCDS6816.1',
                     'GeneID': '23245', 'HGNC': 'HGNC:17021', 'MIM': '612856'}"
                     /product="astrotactin-2 isoform g precursor"
                     /codon_start=1
                     /gene_synonym="bA67K19.1"
                     /transl_table=1
                     /label="ASTN2"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggggagcaa
     1501 gcccctgaaa agattctcag gtctactttc agcttgggcc aaggcctctg gcttcctgtc
     1561 agcaaaagct ttgtggttcc gcctgtggag ctgtccatca accccctggc cagctgcaag
     1621 accgatgtgc tcgtcacgga agaccctgca gatgtcaggg aagaagcgat gctgtccaca
     1681 tactttgaaa ccatcaatga cctgctgtct tccttcgggc cagttcgtga ctgctctcgg
     1741 aacaatgggg gctgcactcg caacttcaag tgtgtgtctg accggcaggt ggattcctcg
     1801 ggatgtgtgt gccctgagga gctgaaaccc atgaaggatg gctctggctg ctacgaccac
     1861 tccaaaggca ttgactgctc tgatggcttt aatggcggct gtgagcagct gtgcctgcag
     1921 cagacgctgc ccctgcccta cgatgccact tcgagcacca tcttcatgtt ctgcggttgc
     1981 gtggaggagt acaaactggc tcctgatgga aaatcctgct taatgctctc agatgtctgc
     2041 gagggcccca agtgcctcaa acctgactcc aaattcaatg ataccctctt tggagagatg
     2101 ctacatggtt acaacaaccg gacccagcat gtgaaccaag gccaagtctt ccagatgacc
     2161 tttagggaga acaacttcat caaggacttt ccccagctgg ccgatgggct gttggtgatc
     2221 ccgctgccgg tggaggagca gtgccggggg gtcctctccg agccccttcc agacctccaa
     2281 ctgctcactg gagatatcag gtatgatgag gccatgggtt accccatggt gcagcagtgg
     2341 cgggtccgga gcaacctcta ccgtgtgaag ctcagcacca tcaccctcgc agcaggcttc
     2401 actaatgttc tcaagatcct gaccaaggag agcagtcggg aggagctgct gtccttcatc
     2461 cagcactatg gctcccacta catcgcagag gccctctatg gctcagagct cacctgcatc
     2521 atccactttc ccagcaagaa ggtccagcag cagctgtggc tccagtatca gaaagagacc
     2581 acagagctgg gcagcaagaa ggagctcaag tccatgccct tcatcaccta cctctcaggt
     2641 ttgctgacag cccagatgct gtcagatgac cagctcattt caggtgtgga gattcgctgt
     2701 gaggagaagg ggcgctgtcc atctacctgt cacctttgcc gccggccagg caaggagcag
     2761 ctgagcccca caccagtgct gctggaaatc aaccgtgtgg tgccacttta taccctcatc
     2821 caagacaatg gcacaaagga ggccttcaag agtgcactga tgagttccta ctggtgctca
     2881 gggaaagggg atgtgatcga tgactggtgc aggtgtgacc tcagcgcctt tgatgccaat
     2941 gggctcccca actgcagccc ccttctgcag ccggtgctgc ggctgtcccc aacagtggag
     3001 ccctccagta ctgtggtctc cttggagtgg gtggatgttc agccagctat tgggaccaag
     3061 gtctccgact atattctgca gcataagaaa gtggatgaat acacagacac tgacctgtac
     3121 acaggagaat tcctgagttt tgctgatgac ttactctctg gcctgggcac atcttgtgta
     3181 gcagctggtc gaagccatgg agaggtccct gaagtcagta tctactcagt catcttcaag
     3241 tgtctggagc ccgacggtct ctacaagttc actctgtatg ctgtggatac acgagggagg
     3301 cactcagagc taagcacggt gaccctgagg acggcctgtc cactggtaga tgacaacaag
     3361 gcagaagaaa tagctgacaa gatctacaat ctgtacaatg ggtacacaag tggaaaggag
     3421 cagcagatgg cctacaacac actgatggag gtctcagcct cgatgctgtt ccgagtccag
     3481 caccactaca actctcacta tgaaaagttt ggcgacttcg tctggagaag tgaggatgag
     3541 ctggggccca ggaaggccca cctgattcta cggcgactgg agagggtgag tagccactgc
     3601 tccagcctcc tgcggagtgc ctacatccag agccgcgtgg aaacagtgcc ctatcttttc
     3661 tgccgcagcg aggaggtccg gcctgcaggc atggtgtggt atagcatcct caaggacacc
     3721 aaaatcacgt gtgaggagaa gatggtgtca atggcccgaa acacgtacgg ggagtccaag
     3781 ggccggggag gtggatctgg ttacccgtat gatgttccgg attacgctgg ctatccctac
     3841 gacgtgcccg actatgccgg gtacccctat gacgtcccag actacgcata actcgagata
     3901 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3961 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     4021 tggctttcat tttctcctcc ttgtataaat cctggttagt tcttgccacg gcggaactca
     4081 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     4141 tggtgtttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatc tagctttatt
     4201 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     4261 aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt
     4321 taaagatctg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga
     4381 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     4441 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
     4501 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     4561 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     4621 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     4681 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4741 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     4801 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     4861 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     4921 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     4981 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     5041 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     5101 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     5161 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     5221 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     5281 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5341 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     5401 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     5461 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     5521 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     5581 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     5641 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     5701 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     5761 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     5821 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     5881 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     5941 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     6001 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     6061 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     6121 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     6181 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     6241 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     6301 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     6361 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     6421 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6481 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6541 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6601 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6661 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6721 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6781 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6841 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6901 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6961 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7021 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     7081 ttgctggcct tttgctcaca tgt
//
