LOCUS       .                       6759 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             3518..3544
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /codon_start=1
                     /transl_table=1
                     /label="HA"
                     /note="color: #cc99b2"
     CDS             3488..3514
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /codon_start=1
                     /transl_table=1
                     /label="HA"
                     /note="color: #cc99b2"
     CDS             3458..3484
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /codon_start=1
                     /transl_table=1
                     /label="HA"
                     /note="color: #cc99b2"
     protein_bind    1367..1400
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /bound_moiety="Cre recombinase"
                     /label="lox2272-JTZ17"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     promoter        4974..5078
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     repeat_region   4022..4162
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     terminator      3548..3979
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     rep_origin      4237..4692
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     rep_origin      6110..6698
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     CDS             5079..5939
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /product="β-lactamase"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     CDS             288..1318
                     /protein_id=""
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /product="site-specific recombinase"
                     /codon_start=1
                     /transl_table=1
                     /label="Cre"
     CDS             1493..3442
                     /protein_id="NP_116750.1"
                     /note="isoform c precursor is encoded by transcript variant
                     c; protocadherin-S; protocadherin 11X; protocadherin on the
                     X chromosome; protein phosphatase 1, regulatory subunit
                     119; Protocadherin-11 Y-linked; Protocadherin on the Y
                     chromosome; Protocadherin prostate cancer;
                     Protocadherin-22; Protocadherin-PC"
                     /note="color: #993366"
                     /translation="VIAVDNDTGMNAEVRYSIVGGNTRDLFAIDQETGNITLMEKCDVT
                     DLGLHRVLVKANDLGQPDSLFSVVIVNLFVNESVTNATLINELVRKSTEAPVTPNTEIA
                     DVSSPTSDYVKILVAAVAGTITVVVVIFITAVVRCRQAPHLKAAQKNKQNSEWATPNPE
                     NRQMIMMKKKKKKKKHSPKNLLLNFVTIEETKADDVDSDGNRVTLDLPIDLEEQTMGKY
                     NWVTTPTTFKPDSPDLARHYKSASPQPAFQIQPETPLNSKHHIIQELPLDNTFVACDSI
                     SKCSSSSSDPYSVSDCGYPVTTFEVPVSVHTRPPMKEVVRSCTPMKESTTMEIWIHPQP
                     QRKSEGKVAGKSQRRVTFHLPEGSQESSSDGGLGDHDAGSLTSTSHGLPLGYPQEEYFD
                     RATPSNRTEGDGNSDPESTFIPGLKKAAEITVQPTVEEASDNCTQECLIYGHSDACWMP
                     ASLDHSSSSQAQASALCHSPPLSQASTQHHSPRVTQTIALCHSPPVTQTIALCHSPPPI
                     QVSALHHSPPLVQATALHHSPPSAQASALCYSPPLAQAAAISHSSPLPQVIALHRSQAQ
                     SSVSLQQGWVQGADGLCSVDQGVQGSATSQFYTMSERLHPSDDSIKVIPLTTFTPRQQA
                     RPSRGDSPIMEEHPL"
                     /gene="PCDH11X"
                     /db_xref="{'GI': '14589920', 'CCDS': 'CCDS14461.1',
                     'GeneID': '27328', 'HGNC': 'HGNC:8656', 'MIM': '300246'}"
                     /product="protocadherin-11 X-linked isoform c precursor"
                     /codon_start=1
                     /gene_synonym="PCDH-X; PCDH-Y; PCDH11; PCDH11Y; PCDH22;
                     PCDHX; PPP1R119"
                     /transl_table=1
                     /label="PCDH11X"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtaattgc
     1501 tgttgacaat gacactggca tgaatgcaga ggttcgttac agcattgtag gaggaaacac
     1561 aagagatctg tttgcaatcg accaagaaac aggcaacata acattgatgg agaaatgtga
     1621 tgttacagac cttggtttac acagagtgtt ggtcaaagct aatgacttag gacagcctga
     1681 ttctctcttc agtgttgtaa ttgtcaatct gttcgtgaat gagtcggtga ccaatgctac
     1741 actgattaat gaactggtgc gcaaaagcac tgaagcacca gtgaccccaa atactgagat
     1801 agctgatgta tcctcaccaa ctagtgacta tgtcaagatc ctggttgcag ctgttgctgg
     1861 caccataact gtcgttgtag ttattttcat cactgctgta gtaagatgtc gccaggcacc
     1921 acaccttaag gctgctcaga aaaacaagca gaattctgaa tgggctaccc caaacccaga
     1981 aaacaggcag atgataatga tgaagaaaaa gaaaaagaag aagaagcatt cccctaagaa
     2041 cttgctgctt aattttgtca ctattgaaga aactaaggca gatgatgttg acagtgatgg
     2101 aaacagagtc acactagacc ttcctattga tctagaagag caaacaatgg gaaagtacaa
     2161 ttgggtaact acacctacta ctttcaagcc cgacagccct gatttggccc gacactacaa
     2221 atctgcctct ccacagcctg ccttccaaat tcagcctgaa actcccctga attcgaagca
     2281 ccacatcatc caagaactgc ctctcgataa cacctttgtg gcctgtgact ctatctccaa
     2341 gtgttcctca agcagttcag atccctacag cgtttctgac tgtggctatc cagtgacgac
     2401 cttcgaggta cctgtgtccg tacacaccag accgccaatg aaggaggttg tgcgatcttg
     2461 cacccccatg aaagagtcta caactatgga gatctggatt catccccaac cacagcggaa
     2521 atctgaaggg aaagtggcag gaaagtccca gcggcgtgtc acatttcacc tgccagaagg
     2581 ctctcaggaa agcagcagtg atggtggact gggagaccat gatgcaggca gccttaccag
     2641 cacatctcat ggcctgcccc ttggctatcc tcaggaggag tactttgatc gtgctacacc
     2701 cagcaatcgc actgaagggg atggcaactc cgatcctgaa tctactttca tacctggact
     2761 aaagaaagct gcagaaataa ctgttcaacc aactgtggaa gaggcctctg acaactgcac
     2821 tcaagaatgt ctcatctatg gccattctga tgcctgctgg atgccggcat ctctggatca
     2881 ttccagctct tcgcaagcac aggcctctgc tctatgccac agcccaccac tgtcacaggc
     2941 ctctactcag caccacagcc cacgagtgac acagaccatt gctctctgcc acagccctcc
     3001 agtgacacag accatcgcat tgtgccacag cccaccaccg atacaggtgt ctgctctcca
     3061 ccacagtcct cctctagtgc aggctactgc acttcaccac agcccaccat cagcacaggc
     3121 ctcagccctc tgctacagcc ctcctttagc acaggctgct gcaatcagcc acagctctcc
     3181 tctgccacag gttattgccc tccatcgtag tcaggcccaa tcatcagtca gtttgcagca
     3241 aggttgggtg caaggtgctg atgggctatg ctctgttgat cagggagtgc aaggtagtgc
     3301 aacatctcag ttttacacca tgtctgaaag acttcatccc agtgatgatt caattaaagt
     3361 cattcctttg acaaccttca ctccacgcca acaggccaga ccgtccagag gtgattcccc
     3421 cattatggaa gaacatccct tgggaggtgg atctggttac ccgtatgatg ttccggatta
     3481 cgctggctat ccctacgacg tgcccgacta tgccgggtac ccctatgacg tcccagacta
     3541 cgcataactc gagataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     3601 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     3661 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttagttctt
     3721 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3781 ggcactgaca attccgtggt gtttatttgt gaaatttgtg atgctattgc tttatttgta
     3841 accatctagc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     3901 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     3961 gatgtgggag gttttttaaa gatctggaga ccggtgtggc acgtgcggac cgagcggccg
     4021 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     4081 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     4141 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
     4201 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     4261 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4321 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4381 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4441 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4501 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4561 cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct
     4621 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     4681 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     4741 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     4801 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     4861 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     4921 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     4981 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5041 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5101 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5161 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5221 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5281 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5341 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5401 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5461 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5521 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5581 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5641 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5701 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5761 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5821 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5881 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5941 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6001 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6061 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6121 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6181 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6241 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6301 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6361 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6421 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6481 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6541 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6601 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6661 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6721 tacggttcct ggccttttgc tggccttttg ctcacatgt
//
