LOCUS       .                       6414 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1493..3115
                     /gene="PXDN"
                     /product="peroxidasin homolog precursor"
                     /EC_number="1.11.1.7"
                     /translation="GIVQRSGKPLLPFATGPPTECMRDENESPIPCFLAGDHRANEQLG
                     LTSMHTLWFREHNRIATELLKLNPHWDGDTIYYETRKIVGAEIQHITYQHWLPKILGEV
                     GMRTLGEYHGYDPGINAGIFNAFATAAFRFGHTLVNPLLYRLDENFQPIAQDHLPLHKA
                     FFSPFRIVNEGGIDPLLRGLFGVAGKMRVPSQLLNTELTERLFSMAHTVALDLAAINIQ
                     RGRDHGIPPYHDYRVYCNLSAAHTFEDLKNEIKNPEIREKLKRLYGSTLNIDLFPALVV
                     EDLVPGSRLGPTLMCLLSTQFKRLRDGDRLWYENPGVFSPAQLTQIKQTSLARILCDNA
                     DNITRVQSDVFRVAEFPHGYGSCDEIPRVDLRVWQDCCEDCRTRGQFNAFSYHFRGRRS
                     LEFSYQEDKPTKKTRPRKIPSVGRQGEHLSNSTSAFSTRSDASGTNDFREFVLEMQKTI
                     TDLRTQIKKLESRLSTTECVDAGGESHANNTKWKKDACTICECKDGQVTCFVEACPPAT
                     CAVPVNIPGACCPVCLQKRAEEKP"
                     /note="peroxidasin homolog; vascular peroxidase 1;
                     p53-responsive gene 2 protein; melanoma-associated antigen
                     MG50; peroxidasin 1"
                     /note="color: #993366"
                     /db_xref="{'GI': '109150416', 'CCDS': 'CCDS46221.1',
                     'GeneID': '7837', 'HGNC': 'HGNC:14966', 'MIM': '605158'}"
                     /gene_synonym="ASGD7; COPOA; D2S448; D2S448E; hsPxd01;
                     MG50; PRG2; PXN; VPO"
                     /codon_start=1
                     /protein_id="NP_036425.1"
                     /transl_table=1
                     /label="PXDN"
     CDS             288..1318
                     /product="site-specific recombinase"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /codon_start=1
                     /protein_id=""
                     /transl_table=1
                     /label="Cre"
     CDS             4734..5594
                     /gene="bla"
                     /product="β-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     rep_origin      5765..6353
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     rep_origin      3892..4347
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     terminator      3203..3634
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   3677..3817
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     promoter        4629..4733
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     CDS             3122..3187
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /codon_start=1
                     /transl_table=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     protein_bind    1367..1400
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /label="lox2272-JTZ17"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac agggcatcgt
     1501 gcagcggtcc gggaagccgc tgctcccctt cgccaccggg ccgcccacgg agtgcatgcg
     1561 ggacgagaac gagagcccca tcccctgctt cctggccggg gaccaccgcg ccaacgagca
     1621 gctgggcctg accagcatgc acacgctgtg gttccgcgag cacaaccgca ttgccacgga
     1681 gctgctcaag ctgaacccgc actgggacgg cgacaccatc tactatgaga ccaggaagat
     1741 cgtgggtgcg gagatccagc acatcaccta ccagcactgg ctcccgaaga tcctggggga
     1801 ggtgggcatg aggacgctgg gagagtacca cggctacgac cccggcatca atgctggcat
     1861 cttcaacgcc ttcgccaccg cggccttcag gtttggccac acgcttgtca acccactgct
     1921 ttaccggctg gacgagaact tccagcccat tgcacaagat cacctccccc ttcacaaagc
     1981 tttcttctct cccttccgga ttgtgaatga gggcggcatc gatccgcttc tcagggggct
     2041 gttcggggtg gcggggaaaa tgcgtgtgcc ctcgcagctg ctgaacacgg agctcacgga
     2101 gcggctgttc tccatggcac acacggtggc tctggacctg gcggccatca acatccagcg
     2161 gggccgggac cacgggatcc caccctacca cgactacagg gtctactgca atctatcggc
     2221 ggcacacacg ttcgaggacc tgaaaaatga gattaaaaac cctgagatcc gggagaaact
     2281 gaaaaggttg tatggctcga cactcaacat cgacctgttt ccggcgctcg tggtggagga
     2341 cctggtgcct ggcagccggc tgggccccac cctgatgtgt cttctcagca cacagttcaa
     2401 gcgcctgcga gatggggaca ggttgtggta tgagaaccct ggggtgttct ccccggccca
     2461 gctgactcag atcaagcaga cgtcgctggc caggatccta tgcgacaacg cggacaacat
     2521 cacccgggtg cagagcgacg tgttcagggt ggcggagttc cctcacggct acggcagctg
     2581 tgacgagatc cccagggtgg acctccgggt gtggcaggac tgctgtgaag actgtaggac
     2641 cagggggcag ttcaatgcct tttcctatca tttccgaggc agacggtctc ttgagttcag
     2701 ctaccaggag gacaagccga ccaagaaaac aagaccacgg aaaataccca gtgttgggag
     2761 acagggggaa catctcagca acagcacctc agccttcagc acacgctcag atgcatctgg
     2821 gacaaatgac ttcagagagt ttgttctgga aatgcagaag accatcacag acctcagaac
     2881 acagataaag aaacttgaat cacggctcag taccacagag tgcgtggatg ccgggggcga
     2941 atctcacgcc aacaacacca agtggaaaaa agatgcatgc accatttgtg aatgcaaaga
     3001 cgggcaggtc acctgcttcg tggaagcttg cccccctgcc acctgtgctg tccccgtgaa
     3061 catcccaggg gcctgctgtc cagtctgctt acagaagagg gcggaggaaa agccctgtac
     3121 agactacaaa gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga
     3181 tgacaagtaa gaattctgat gactcgagat aatcaacctc tggattacaa aatttgtgaa
     3241 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3301 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3361 tcctggttag ttcttgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     3421 ggggctcggc tgttgggcac tgacaattcc gtggtgttta tttgtgaaat ttgtgatgct
     3481 attgctttat ttgtaaccat ctagctttat ttgtgaaatt tgtgatgcta ttgctttatt
     3541 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     3601 tcaggttcag ggggagatgt gggaggtttt ttaaagatct ggagaccggt gtggcacgtg
     3661 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     3721 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     3781 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
     3841 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     3901 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3961 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4021 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4081 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     4141 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4201 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt
     4261 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4321 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     4381 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     4441 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     4501 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     4561 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     4621 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4681 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4741 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4801 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4861 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4921 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4981 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5041 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5101 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5161 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5221 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5281 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5341 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5401 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5461 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5521 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5581 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5641 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5701 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5761 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5821 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5881 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     5941 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6001 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6061 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6121 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6181 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6241 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6301 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6361 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
