LOCUS       .                       6671 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    1367..1400
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /label="lox2272-JTZ17"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     CDS             3379..3444
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /codon_start=1
                     /transl_table=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     promoter        4886..4990
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     repeat_region   3934..4074
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     terminator      3460..3891
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     rep_origin      4149..4604
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     rep_origin      6022..6610
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     CDS             4991..5851
                     /gene="bla"
                     /product="β-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     CDS             288..1318
                     /product="site-specific recombinase"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /codon_start=1
                     /protein_id=""
                     /transl_table=1
                     /label="Cre"
     CDS             1493..3372
                     /gene="CECR2"
                     /product="chromatin remodeling regulator CECR2 isoform 1"
                     /translation="YMRPPCKSAGHRLQPPPVPAPSSLFGAPAQALRGVQGGDSMMDSP
                     EMIAMQQLSSRVCPPGVPYHPHQPAHPRLPGPFPQVAHPMSVTVSAPKPALGNPGRAPE
                     NSEAQEPENDQAEPLPGLEEKPPGVGTSEGVYLTQLPHPTPPLQTDCTRQSSPQERETV
                     GPELKSSSSESADNCKAMKGKNPWPSDSSYPGPAAQGCVRDLSTVADRGALSENGVIGE
                     ASPCGSEGKGLGSSGSEKLLCPRGRTLQETMPCTGQNAATPPSTDPGLTGGTVSQFPPL
                     YMPGLEYPNSAAHYHISPGLQGVGPVMGGKSPASHPQHFPPRGFQSNHPHSGGFPRYRP
                     PQGMRYSYHPPPQPSYHHYQRTPYYACPQSFSDWQRPLHPQGSPSGPPASQPPPPRSLF
                     SDKNAMASLQGCETLNAALTSPTRMDAVAAKVPNDGQNPGPEEEKLDESMERPESPKEF
                     LDLDNHNAATKRQSSLSASEYLYGTPPPLSSGMGFGSSAFPPHSVMLQTGPPYTPQRPA
                     SHFQPRAYSSPVAALPPHHPGATQPNGLSQEGPIYRCQEEGLGHFQAVMMEQIGTRSGI
                     RGPFQEMYRPSGMQMHPVQSQASFPKTPTAATSQEEVPPHKPPTLPLDQS"
                     /note="isoform 1 is encoded by transcript variant 1; cat
                     eye syndrome critical region protein 2; cat eye syndrome
                     chromosome region, candidate 2; chromatin remodeling
                     regulator CECR2"
                     /note="color: #993366"
                     /db_xref="{'GI': '586798144', 'CCDS': 'CCDS93111.1',
                     'GeneID': '27443', 'HGNC': 'HGNC:1840', 'MIM': '607576'}"
                     /codon_start=1
                     /protein_id="NP_001276976.1"
                     /transl_table=1
                     /label="CECR2"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac agggtacatg
     1501 cgaccgccct gcaagtctgc cggacatcgg ttacagccac ctccagtgcc agcacccagt
     1561 tctttgtttg gagcacctgc ccaggctctt cggggggtgc agggagggga ctccatgatg
     1621 gacagcccag agatgattgc gatgcagcag ctctcctccc gcgtctgccc cccaggtgtg
     1681 ccttaccacc cccaccagcc tgcacacccc cgtttacctg gcccttttcc gcaggtagct
     1741 cacccaatgt cagtcactgt gtcagccccc aagcctgccc tgggcaaccc tgggagggca
     1801 ccggagaaca gtgaagcaca agagcctgag aatgaccaag cagagccgtt gcctggcctt
     1861 gaagagaaac caccaggtgt tggtacttca gagggggtct acctcacaca actacctcac
     1921 cccacacctc ccctgcagac tgactgcacc aggcagagct caccacaaga aagggaaaca
     1981 gtgggcccgg agctcaaaag cagctcctcc gaatctgcgg acaactgtaa agcaatgaag
     2041 ggcaagaatc cctggccctc ggatagcagc taccccggcc cagccgccca agggtgcgtg
     2101 agagacctct ccacggtggc agacaggggc gctctatccg agaacggagt cattggggaa
     2161 gcatctcctt gtggatcgga ggggaagggc cttggtagca gtggttccga aaagctgctc
     2221 tgccccagag gcagaacgtt gcaggaaacc atgccatgca cgggacagaa cgcagcgaca
     2281 ccgcccagca cagaccccgg tttgacggga ggcactgtga gccagtttcc cccgctgtat
     2341 atgcctggcc tagagtaccc gaattcagct gcccattacc acatcagtcc aggcctgcag
     2401 ggtgtgggcc ctgtgatggg agggaagtcc ccagcatccc atccccagca ttttccccca
     2461 aggggctttc agtctaacca cccacattct ggaggctttc cccggtatcg ccccccacaa
     2521 ggaatgaggt attcctacca cccaccgcca cagccttcct accaccacta tcagcgaact
     2581 ccttactatg cctgtccaca gagcttttct gactggcaga gacctctcca tccccaggga
     2641 agcccaagcg gacccccagc cagtcagcct cccccaccaa ggtccctctt ctcagataag
     2701 aatgccatgg ccagtctgca aggctgtgag acactgaatg ctgccttaac ttctccaacc
     2761 cgtatggatg cagtggctgc taaagtccca aatgacgggc agaatcctgg tccagaggaa
     2821 gagaagctgg atgaatctat ggagaggcca gagagtccca aagaattttt agacctggac
     2881 aaccataacg cagctaccaa gcggcagagc tcgttgtcag ccagcgagta tctctatgga
     2941 actcctccgc ctctgagttc aggaatggga tttggttcat ctgcatttcc accccacagt
     3001 gtgatgctgc agacggggcc tccctatacc cctcagcggc cggccagtca ctttcagccc
     3061 agggcttact cttcccctgt ggctgccctc ccacctcacc acccaggggc cacccagccc
     3121 aacggcctct ctcaggaggg tcccatctat cgctgccagg aagaaggcct gggtcacttt
     3181 caagctgtga tgatggaaca aattggcact agaagtggaa taagaggacc tttccaggaa
     3241 atgtacagac catcaggaat gcagatgcac ccggtccagt cgcaggcctc gttcccaaag
     3301 acccccacag cagcaacatc acaggaggag gtgccgcctc ataagcctcc aacacttccc
     3361 ctggatcaga gctgtacaga ctacaaagac catgacggtg attataaaga tcatgacatc
     3421 gactacaagg atgacgatga caagtaagaa ttctgatgac tcgagataat caacctctgg
     3481 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     3541 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     3601 tctcctcctt gtataaatcc tggttagttc ttgccacggc ggaactcatc gccgcctgcc
     3661 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgtttattt
     3721 gtgaaatttg tgatgctatt gctttatttg taaccatcta gctttatttg tgaaatttgt
     3781 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     3841 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta aagatctgga
     3901 gaccggtgtg gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca
     3961 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     4021 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
     4081 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
     4141 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4201 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4261 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4321 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     4381 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4441 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt
     4501 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4561 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     4621 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     4681 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     4741 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     4801 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     4861 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4921 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4981 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5041 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5101 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5161 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5221 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5281 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5341 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5401 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5461 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5521 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5581 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5641 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5701 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5761 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5821 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     5881 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     5941 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6001 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6061 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6121 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     6181 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6241 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6301 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6361 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6421 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6481 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6541 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6601 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6661 tgctcacatg t
//
