LOCUS       .                       6140 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             760..3280
                     /protein_id="NP_056082.2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     homeobox protein cut-like 2; homeobox protein cux-2"
                     /note="color: #993366"
                     /translation="MAANVGSMFQYWKRFDLRRLQKELNSVASELSARQEESEHSHKHL
                     IELRREFKKNVPEEIREMVAPVLKSFQAEVVALSKRSQEAEAAFLSVYKQLIEAPDPVP
                     VFEAARSLDDRLQPPSFDPSGQPRRDLHTSWKRNPELLSPKEQREGTSPAGPTLTEGSR
                     LPGIPGKALLTETLLQRNEAEKQKGLQEVQITLAARLGEAEEKIKVLHSALKATQAELL
                     ELRRKYDEEAASKADEVGLIMTNLEKANQRAEAAQREVESLREQLASVNSSIRLACCSP
                     QGPSGDKVNFTLCSGPRLEAALASKDREILRLLKDVQHLQSSLQELEEASANQIADLER
                     QLTAKSEAIEKLEEKLQAQSDYEEIKTELSILKAMKLASSTCSLPQGMAKPEDSLLIAK
                     EAFFPTQKFLLEKPSLLASPEEDPSEDDSIKDSLGTEQSYPSPQQLPPPPGPEDPLSPS
                     PGQPLLGPSLGPDGTRTFSLSPFPSLASGERLMMPPAAFKGEAGGLLVFPPAFYGAKPP
                     TAPATPAPGPEPLGGPEPADGGGGGAAGPGAEEEQLDTAEIAFQVKEQLLKHNIGQRVF
                     GHYVLGLSQGSVSEILARPKPWRKLTVKGKEPFIKMKQFLSDEQNVLALRTIQVRQRGS
                     ITPRIRTPETGSDDAIKSILEQAKKEIESQKGGEPKTSVAPLSIANGTTPASTSEDAIK
                     SILEQARREMQAQQQALLEMEVAPRGRSVPPSPPERPSLATASQNGAPALVKQEEGSGG
                     PAQAPLPVLSPAAFVQSIIRKVKSEIGDAGYFDHHWASDRGLLSRPYASVSPSLSSSSS
                     SGYSGQPNGRAWPRGDEAPVPPEDEAAA"
                     /gene="CUX2"
                     /db_xref="{'GI': '116517292', 'CCDS': 'CCDS41837.1',
                     'GeneID': '23316', 'HGNC': 'HGNC:19347', 'MIM': '610648'}"
                     /product="homeobox protein cut-like 2 isoform 1"
                     /codon_start=1
                     /gene_synonym="CDP2; CUTL2; DEE67; EIEE67"
                     /transl_table=1
                     /label="CUX2"
     CDS             4460..5320
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /product="β-lactamase"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     rep_origin      5491..6079
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      3618..4073
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   3403..3543
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     promoter        4355..4459
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          3281..3321
                     /label="Splicing Donor"
                     /note="color: noColor"
     misc_feature    3325..3358
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     CDS             727..753
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /codon_start=1
                     /transl_table=1
                     /label="HA"
                     /note="color: #cc99b2"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatcta tggccgccaa tgtgggatcg
      781 atgtttcaat attggaagcg atttgatcta cggcgactcc agaaggagct taattccgtc
      841 gcttctgagc tgtctgcacg gcaggaggag agtgaacatt ctcataaaca tttaattgaa
      901 ctccgccggg aatttaagaa aaatgtacct gaggaaatca gagagatggt ggctcctgta
      961 ttaaaaagct tccaagccga ggtggtggcc cttagtaaga gaagtcagga ggcggaggct
     1021 gcttttctga gtgtttacaa gcaattaatt gaagcaccag accccgtgcc tgtgtttgag
     1081 gcggcacgca gcctagacga cagactgcag ccccccagct ttgaccccag tgggcagccc
     1141 cggcgagacc tccacacttc gtggaagagg aaccccgagc tcctcagccc caaagagcag
     1201 agagagggga cgtcgcctgc cgggcccacg ctgaccgagg gaagccgcct cccaggcatt
     1261 cccgggaaag ccctcctgac agaaaccttg ctgcagagaa atgaggcgga aaaacaaaag
     1321 ggccttcaag aagtacagat cactttggcg gccagactgg gggaggcaga ggagaaaatc
     1381 aaagtcctac attcagcgct aaaggctacg caggcagagc tgctagagct gcggcggaag
     1441 tacgacgagg aggcagcatc caaggcagat gaagtcggcc tgatcatgac caacctggag
     1501 aaagctaatc agcgagctga ggctgcccag cgggaggtgg aaagtctccg ggaacagctg
     1561 gcctctgtca acagctccat ccgcctggct tgctgctctc cccaggggcc cagtggggat
     1621 aaggtgaact tcactctgtg ctcgggccct cggctggagg ccgcgctggc ctccaaggac
     1681 agggagatcc tgcggctgct gaaggacgtg cagcacctcc agagctcact gcaggagctg
     1741 gaggaggcat ccgccaacca gatcgccgac ctggagcggc agctcacggc caagtccgag
     1801 gccatagaaa agctggaaga gaagctccag gcccagtctg actatgagga aattaaaacg
     1861 gagctgagca tcctgaaagc catgaagctg gcctccagca cctgcagcct cccccagggc
     1921 atggccaagc ctgaagactc actgcttatt gcaaaggagg ccttcttccc cacgcagaaa
     1981 ttccttctgg agaagcccag cctcctggcc agccctgagg aagacccatc agaggacgat
     2041 tccatcaagg attcactggg cacggagcag tcctacccct cccctcagca gctcccacct
     2101 ccaccagggc cagaagaccc cctgtctccc agccccgggc agcccctgct gggccccagc
     2161 ttggggcctg acggcactcg gactttctcg ctgtccccct tccccagcct ggcatcaggg
     2221 gagagactga tgatgccccc agccgccttc aagggagagg cgggcggcct gctggtgttc
     2281 cccccagcct tctatggcgc caagcccccc acagcccctg ccaccccggc ccctggccct
     2341 gagccactgg gcggtcctga gcccgcggat ggtggtgggg gcggagcggc ggggcccggg
     2401 gcagaggagg agcagctgga cacggcagag atcgccttcc aggtgaagga gcagctgctg
     2461 aaacacaaca tcgggcagcg ggtgtttggg cattacgtgc tggggctgtc gcagggctcg
     2521 gtcagcgaga tcctagcccg gcccaagccc tggcgcaagc tcacggtgaa gggcaaggag
     2581 cccttcatca agatgaagca gttcctgtcg gatgagcaga atgtactggc gctcaggacc
     2641 atccaagtgc ggcagcgagg cagcatcacc ccgagaatcc gcacgcctga gacaggctca
     2701 gacgacgcca tcaagagcat tctagagcag gccaagaagg agatcgagtc gcagaagggc
     2761 ggcgagccca agacctcggt ggccccgctg agcatcgcca acggcacgac ccccgccagc
     2821 acctcggagg acgccatcaa gagcatcctg gagcaggcac gccgtgagat gcaggcgcaa
     2881 cagcaggcgc tgctggagat ggaggtggcg cccaggggcc gctcggtgcc cccctcgccc
     2941 ccggagcggc catcactggc caccgcgagc cagaacgggg ccccggcctt ggtgaagcag
     3001 gaggagggca gcgggggccc cgcgcaggcg ccgctcccgg tcctgtcccc cgccgccttc
     3061 gtgcagagca tcatccgcaa ggtcaagtcc gagatcggcg acgccggcta cttcgaccac
     3121 cactgggcct ccgaccgcgg cctgctcagc cgcccctacg cctccgtgtc gccctcgctg
     3181 tcctcctcct cctcctctgg ctactctggc cagcccaacg gccgcgcctg gccccgcggg
     3241 gacgaggccc ctgtgccccc cgaggacgag gcggcggcag gtaagtatca aggttacaag
     3301 acaggtttaa ggagaccaat aagctattat tcgtatagga tactttatac gaagttatta
     3361 agttatggag accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg
     3421 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     3481 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     3541 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     3601 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     3661 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     3721 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     3781 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     3841 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     3901 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg
     3961 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     4021 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     4081 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     4141 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     4201 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     4261 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4321 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4381 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4441 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4501 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4561 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4621 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4681 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4741 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4801 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4861 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4921 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4981 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5041 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5101 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5161 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5221 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5281 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5341 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5401 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5461 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5521 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5581 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     5641 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5701 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5761 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5821 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5881 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5941 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6001 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6061 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6121 ctggcctttt gctcacatgt
//
