LOCUS       .                       6978 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    1367..1400
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /bound_moiety="Cre recombinase"
                     /label="lox2272-JTZ17"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     CDS             3698..3763
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /codon_start=1
                     /transl_table=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     promoter        5193..5297
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     repeat_region   4241..4381
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     terminator      3767..4198
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     rep_origin      4456..4911
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     rep_origin      6329..6917
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     CDS             5298..6158
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /product="β-lactamase"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     CDS             288..1318
                     /protein_id=""
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /product="site-specific recombinase"
                     /codon_start=1
                     /transl_table=1
                     /label="Cre"
     CDS             1493..3691
                     /protein_id="NP_077816.1"
                     /note="ATPase, Class V, type 10C; aminophospholipid
                     translocase VA; ATPase, class V, type 10A; ATPase type IV,
                     phospholipid transporting (P-type); P4-ATPase flippase
                     complex alpha subunit ATP10A; probable
                     phospholipid-transporting ATPase VA"
                     /note="color: #993366"
                     /translation="GADSVVMDLLQPCSSVDARGRHQKKIRSKTQNYLNVYAAEGLRTL
                     CIAKRVLSKEEYACWLQSHLEAESSLENSEELLFQSAIRLETNLHLLGATGIEDRLQDG
                     VPETISKLRQAGLQIWVLTGDKQETAVNIAYACKLLDHDEEVITLNATSQEACAALLDQ
                     CLCYVQSRGLQRAPEKTKGKVSMRFSSLCPPSTSTASGRRPSLVIDGRSLAYALEKNLE
                     DKFLFLAKQCRSVLCCRSTPLQKSMVVKLVRSKLKAMTLAIGDGANDVSMIQVADVGVG
                     ISGQEGMQAVMASDFAVPKFRYLERLLILHGHWCYSRLANMVLYFFYKNTMFVGLLFWF
                     QFFCGFSASTMIDQWYLIFFNLLFSSLPPLVTGVLDRDVPANVLLTNPQLYKSGQNMEE
                     YRPRTFWFNMADAAFQSLVCFSIPYLAYYDSNVDLFTWGTPIVTIALLTFLLHLGIETK
                     TWTWLNWITCGFSVLLFFTVALIYNASCATCYPPSNPYWTMQALLGDPVFYLTCLMTPV
                     AALLPRLFFRSLQGRVFPTQLQLARQLTRKSPRRCSAPKETFAQGRLPKDSGTEHSSGR
                     TVKTSVPLSQPSWHTQQPVCSLEASGEPSTVDMSMPVREHTLLEGLSAPAPMSSAPGEA
                     VLRSPGGCPEESKVRAASTGRVTPLSSLFSLPTFSLLNWISSWSLVSRLGSVLQFSRTE
                     QLADGQAGRGLPVQPHSGRSGLQGPDHRLLIGASSRRSQ"
                     /gene="ATP10A"
                     /EC_number="7.6.2.1"
                     /db_xref="{'GI': '14424433', 'CCDS': 'CCDS32178.1',
                     'GeneID': '57194', 'HGNC': 'HGNC:13542', 'MIM': '605855'}"
                     /product="phospholipid-transporting ATPase VA"
                     /codon_start=1
                     /gene_synonym="ATP10C; ATPVA; ATPVC"
                     /transl_table=1
                     /label="ATP10A"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac agggggccga
     1501 ctcagtggtc atggatctcc tgcagccctg ctcttcagtt gacgccagag ggaggcatca
     1561 aaaaaagatt cggagcaaaa ctcagaatta cctcaacgtg tatgcggcgg aaggcctgcg
     1621 caccttgtgc atcgccaaga gagttctgag taaagaagag tatgcctgct ggttgcaaag
     1681 ccacctagaa gccgaatcct ccctggaaaa cagcgaggag ctcctcttcc agtctgccat
     1741 tcgcctggag accaacctgc acttgttagg tgccactggg attgaagacc gcctgcagga
     1801 cggagtccct gaaactattt ctaaattgcg tcaagcgggc ctgcagattt gggttctcac
     1861 tggtgacaaa caagaaacag ctgtcaacat tgcatatgcc tgcaaactgc tggaccacga
     1921 cgaggaggtc atcaccctga atgccacctc ccaggaggcg tgtgcagccc tgctagacca
     1981 gtgcctatgc tacgtgcagt ccagaggcct ccagagagcc cctgagaaga ccaagggcaa
     2041 agtgagcatg aggttctcct ctctctgccc accctccacg tccactgcct ctggccgcag
     2101 acccagcctc gtgatcgatg ggagaagcct ggcctacgct ctcgagaaaa acctggagga
     2161 caaattcctc ttccttgcca agcagtgccg ctccgtcctc tgctgtcggt cgacgcctct
     2221 gcagaagagc atggtggtga agctggtgcg gagcaagctc aaggccatga ccctggccat
     2281 aggtgatgga gccaatgatg tcagcatgat ccaggtggca gatgtgggtg tgggaatctc
     2341 cggccaggag ggtatgcagg cagtgatggc cagcgacttt gcagtgccga aattccgata
     2401 cctggagagg ctcttgattc ttcacgggca ttggtgctac tcccgacttg ccaacatggt
     2461 gctgtacttc ttctacaaaa acacaatgtt cgtgggcctc ctgttttggt tccagttttt
     2521 ctgtggcttc tctgcatcta ccatgattga ccagtggtat ctaatcttct ttaatctgct
     2581 cttctcgtca cttcccccgc tcgtgactgg ggtgctggac agggatgtgc cagccaatgt
     2641 gctgctgacc aacccgcagc tctacaagag tggccagaac atggaggaat accggccacg
     2701 aacgttctgg tttaacatgg ccgacgccgc cttccagagc ctggtttgct tttccattcc
     2761 ttacctggcc tactatgact cgaacgtgga cctgtttacc tgggggaccc ctattgtgac
     2821 aatcgcgctg ctcactttcc tgctccacct gggcattgaa accaaaacct ggacctggct
     2881 caactggata acgtgtggct tcagtgtcct tttgtttttc accgtggctt tgatttacaa
     2941 tgcgtcttgt gccacgtgct atcctccgtc caacccttac tggactatgc aagccttact
     3001 gggtgaccca gtgttttact tgacttgcct gatgacgcct gtcgctgcac tgctgcccag
     3061 attgtttttc agatccctcc aggggagggt tttccccaca caacttcagc tggcacgtca
     3121 gttgaccagg aagtccccca ggagatgcag tgctcccaaa gagacctttg ctcagggacg
     3181 cctcccgaag gactcgggaa ccgagcactc atcagggagg acagtcaaga cctctgtgcc
     3241 cctgtcccag ccttcttggc acacacagca gccggtctgc tccctggagg ccagcgggga
     3301 gcccagcaca gtggacatga gcatgccagt gagggagcac accctgctgg aggggctgag
     3361 cgcaccggcc cccatgtcct ctgcgccagg ggaggctgtc ctgaggagtc caggagggtg
     3421 tcctgaggag tccaaggtga gagctgccag caccggcagg gtgacccccc tgtcttccct
     3481 cttcagcctg cctaccttca gcttactcaa ctggatttcc tcctggtcgc tggtcagcag
     3541 gctggggagt gtcttacagt tctcccggac ggagcagctt gcagatggac aagcgggacg
     3601 tggacttcct gtccagcccc actcaggccg atcaggactt caagggccag accacagact
     3661 acttatagga gcatcttcaa ggcggtcaca gtgtacagac tacaaagacc atgacggtga
     3721 ttataaagat catgacatcg actacaagga tgacgatgac aagtaactcg agataatcaa
     3781 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     3841 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3901 ttcattttct cctccttgta taaatcctgg ttagttcttg ccacggcgga actcatcgcc
     3961 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     4021 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccatctagct ttatttgtga
     4081 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     4141 caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag
     4201 atctggagac cggtgtggca cgtgcggacc gagcggccgc aggaacccct agtgatggag
     4261 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
     4321 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
     4381 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     4441 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4501 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     4561 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     4621 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     4681 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4741 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct
     4801 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     4861 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     4921 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     4981 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     5041 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     5101 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     5161 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5221 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5281 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5341 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5401 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5461 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5521 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5581 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5641 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5701 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5761 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5821 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5881 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5941 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6001 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6061 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6121 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6181 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6241 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6301 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6361 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6421 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     6481 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6541 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6601 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6661 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6721 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6781 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6841 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6901 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6961 ggccttttgc tcacatgt
//
