LOCUS       .                       7241 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             760..4381
                     /protein_id="NP_001025226.1"
                     /note="isoform a is encoded by transcript variant 1; growth
                     factor independent 2; rho GTPase-activating protein 5; p105
                     RhoGAP; p100 RasGAP-associated p105 protein; rho-type
                     GTPase-activating protein 5"
                     /note="color: #993366"
                     /translation="MAKNKEPRPPSYTISVVGLSGTEKDKGNCGVGKSCLCNRFVRSKA
                     DEYYPEHTSVLSTIDFGGRVVNNDHFLYWGDIIQNSEDGVECKIHVIEQTEFIDDQTFL
                     PHRSTNLQPYIKRAAASKLQSAEKLMYICTDQLGLEQDFEQKQMPEGKLNVDGFLLCID
                     VSQGCNRKFDDQLKFVNNLFVQLSKSKKPVIIAATKCDECVDHYLREVQAFASNKKNLL
                     VVETSARFNVNIETCFTALVQMLDKTRSKPKIIPYLDAYKTQRQLVVTATDKFEKLVQT
                     VRDYHATWKTVSNKLKNHPDYEEYINLEGTRKARNTFSKHIEQLKQEHIRKRREEYINT
                     LPRAFNTLLPNLEEIEHLNWSEALKLMEKRADFQLCFVVLEKTPWDETDHIDKINDRRI
                     PFDLLSTLEAEKVYQNHVQHLISEKRRVEMKEKFKKTLEKIQFISPGQPWEEVMCFVME
                     DEAYKYITEADSKEVYGRHQREIVEKAKEEFQEMLFEHSELFYDLDLNATPSSDKMSEI
                     HTVLSEEPRYKALQKLAPDRESLLLKHIGFVYHPTKETCLSGQNCTDIKVEQLLASSLL
                     QLDHGRLRLYHDSTNIDKVNLFILGKDGLAQELANEIRTQSTDDEYALDGKIYELDLRP
                     VDAKSPYFLSQLWTAAFKPHGCFCVFNSIESLSFIGEFIGKIRTEASQIRKDKYMANLP
                     FTLILANQRDSISKNLPILRHQGQQLANKLQCPFVDVPAGTYPRKFNETQIKQALRGVL
                     ESVKHNLDVVSPIPANKDLSEADLRIVMCAMCGDPFSVDLILSPFLDSHSCSAAQAGQN
                     NSLMLDKIIGEKRRRIQITILSYHSSIGVRKDELVHGYILVYSAKRKASMGMLRAFLSE
                     VQDTIPVQLVAVTDSQADFFENEAIKELMTEGEHIATEITAKFTALYSLSQYHRQTEVF
                     TLFFSDVLEKKNMIENSYLSDNTRESTHQSEDVFLPSPRDCFPYNNYPDSDDDTEAPPP
                     YSPIGDDVQLLPTPSDRSRYRLDLEGNEYPIHSTPNCHDHERNHKVPPPIKPKPVVPKT
                     NVKKLDPNLLKTIEAGIGKNPRKQTSRVPLAHPEDMDPSDNYAEPIDTIFKQKGYSDEI
                     YVVPDDSQNRIKIRNSFVNNTQGDEENGFSDRTSKSHGERRPSKYKYKSKTLFSKAKSY
                     YRRTHSDASDDEAFTTSKTKRKGRHRGSEEDPLLSPVETWK"
                     /gene="ARHGAP5"
                     /db_xref="{'GI': '71834863', 'CCDS': 'CCDS32062.1',
                     'GeneID': '394', 'HGNC': 'HGNC:675', 'MIM': '602680'}"
                     /product="rho GTPase-activating protein 5 isoform a"
                     /codon_start=1
                     /gene_synonym="GFI2; p190-B; p190BRhoGAP; RhoGAP5"
                     /transl_table=1
                     /label="ARHGAP5"
     CDS             5561..6421
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /product="β-lactamase"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     rep_origin      6592..7180
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      4719..5174
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   4504..4644
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     promoter        5456..5560
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          4382..4422
                     /label="Splicing Donor"
                     /note="color: noColor"
     misc_feature    4426..4459
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     CDS             727..753
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /codon_start=1
                     /transl_table=1
                     /label="HA"
                     /note="color: #cc99b2"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatcta tggcaaaaaa caaagagcct
      781 cgtcccccat cctataccat cagtgtagtt ggactctctg ggactgaaaa agacaaaggt
      841 aactgtggag ttggaaagtc ttgtttgtgc aatagatttg tacgctcaaa agcagatgaa
      901 tattatccag agcatacttc tgtgcttagc accattgact ttggaggacg agtagtaaac
      961 aatgatcact ttttgtactg gggtgacata atacaaaata gtgaagatgg agtagaatgc
     1021 aaaattcatg tcattgaaca aacagagttc attgatgacc agactttctt gcctcatcgg
     1081 agtacgaatt tgcaaccata tataaaacgt gcagctgcat ctaaattgca gtcagcagaa
     1141 aaactaatgt acatttgcac tgatcagcta ggcttagaac aagactttga acagaagcaa
     1201 atgcctgaag ggaagctcaa cgtagatgga tttttattat gcattgatgt aagtcaagga
     1261 tgcaatagga agtttgatga tcaacttaaa tttgtgaata acctttttgt ccagttatca
     1321 aaatcaaaaa aacctgtaat aatagcagca actaaatgtg atgaatgcgt ggatcattat
     1381 cttagagaag ttcaggcatt tgcttcaaat aaaaagaacc ttcttgtagt ggaaacatca
     1441 gcacgattta atgtcaacat tgaaacatgt tttactgcac tggtacaaat gttggataaa
     1501 actcgtagca agcctaaaat tattccctat ttggatgctt ataaaacaca gagacaactt
     1561 gttgtcacag caacagataa gtttgaaaaa cttgtgcaga ctgtgagaga ttatcatgca
     1621 acttggaaaa ctgttagtaa taaattaaaa aatcatcctg attatgaaga atacatcaac
     1681 ttagagggaa caagaaaggc cagaaataca ttctcaaaac atatagaaca acttaaacag
     1741 gaacatataa gaaaaaggag agaagagtat ataaatactt taccaagagc ttttaacact
     1801 cttttgccaa atctagaaga gattgaacat ttgaattggt cagaagcttt gaagttaatg
     1861 gaaaagagag cagatttcca gttatgtttt gtggtgctag aaaaaactcc ttgggatgaa
     1921 actgaccata tagacaaaat taatgatagg cggattccat ttgacctcct gagcacttta
     1981 gaagctgaaa aagtctatca gaaccatgta cagcatctga tatccgagaa gaggagggtg
     2041 gaaatgaagg aaaaattcaa aaagactttg gaaaaaattc aattcatttc accagggcag
     2101 ccatgggagg aagttatgtg ctttgttatg gaggatgaag cctacaaata tatcactgag
     2161 gctgatagca aagaggtata tggtaggcat cagcgagaaa tagttgaaaa agccaaagaa
     2221 gagtttcaag aaatgctttt tgagcattct gaactttttt atgatttaga tcttaatgca
     2281 acacctagtt cagataaaat gagtgaaatt catacagttc tgagtgaaga acctagatat
     2341 aaagctttac agaaacttgc acctgatagg gaatcccttc tacttaagca tataggattt
     2401 gtttatcatc ccactaaaga aacatgtctt agtggccaaa attgtacaga cattaaagtg
     2461 gagcagttac ttgctagtag tcttttacag ttggatcatg gccgcttaag attatatcac
     2521 gatagtacca atatagataa agttaacctt tttattttag ggaaggatgg ccttgcccaa
     2581 gaactagcaa atgagataag gacacaatcc actgatgatg agtatgcctt agatggaaaa
     2641 atttatgaac ttgatcttcg gccggttgat gccaaatcgc cttacttttt gagtcagtta
     2701 tggactgccg cctttaaacc acatgggtgc ttctgtgtat ttaattccat tgagtcattg
     2761 agttttattg gggaatttat tgggaaaata agaactgaag cttctcagat cagaaaagat
     2821 aaatacatgg ctaatcttcc atttacatta attctggcta atcagagaga ttccattagt
     2881 aagaatctac caattctcag gcaccaaggg cagcagttgg caaacaagtt gcaatgtcct
     2941 tttgtagatg tacctgctgg tacatatcct cgtaaattta atgaaaccca aataaagcaa
     3001 gctctcagag gagtattgga atcagttaaa cacaatttgg atgtggtgag cccaattcct
     3061 gccaataagg acttatcaga agctgacttg agaattgtca tgtgcgccat gtgtggagat
     3121 ccatttagtg tggatcttat tctttcaccc ttccttgatt ctcattcttg cagtgctgct
     3181 caagctggac agaataattc cctaatgctt gataaaatca ttggtgaaaa aaggaggcga
     3241 atacagatca caatattatc ataccactct tcaattggag taagaaaaga tgaactagtt
     3301 catgggtata tattagttta ctctgcaaaa cggaaagctt cgatgggaat gcttcgagca
     3361 tttctatcag aagttcaaga caccattcct gtacagctgg tggcagttac tgacagccaa
     3421 gcagattttt ttgaaaatga ggctatcaaa gagttaatga ctgaaggaga acacattgca
     3481 actgagatca ctgctaaatt tacagcactg tattctttat ctcagtatca tcggcaaact
     3541 gaggtcttta ctctgttttt tagtgatgtt ctagagaaaa aaaatatgat agaaaattct
     3601 tatttgtctg ataatacaag ggaatcaacc catcaaagtg aagatgtttt tctaccatct
     3661 cccagagact gttttcccta taataactac cctgattcag atgatgacac agaagcacca
     3721 cctccttata gtccaattgg ggatgatgta cagttgcttc caacacctag tgaccgttcc
     3781 agatatagat tagatttgga aggaaatgag tatcctattc atagtacccc aaactgtcat
     3841 gaccatgaac gcaaccataa agtgcctcca cctattaaac ctaaaccagt tgtacctaag
     3901 acaaatgtga aaaaactcga tccaaacctt ttaaaaacaa ttgaagctgg tattggtaaa
     3961 aatccaagaa agcagacttc ccgggtgcct ttggcacatc ctgaagatat ggatccttca
     4021 gataactatg cggaacccat tgatacaatt ttcaaacaga agggctattc tgatgagatt
     4081 tatgttgtcc cagatgatag tcaaaatcgt attaaaattc gaaactcatt tgtaaataac
     4141 acccaaggag atgaagaaaa tgggttttct gatagaacct caaaaagtca tggggaacgg
     4201 aggccttcaa aatacaaata taaatctaaa accttgttta gtaaagccaa gtcatactat
     4261 agaagaacac attcagatgc cagtgatgat gaggctttca ccacttctaa aacaaaaaga
     4321 aaaggaagac atcgtggaag tgaagaagat ccacttcttt ctcctgttga aacttggaaa
     4381 ggtaagtatc aaggttacaa gacaggttta aggagaccaa taagctatta ttcgtatagg
     4441 atactttata cgaagttatt aagttatgga gaccggtgtg gcacgtgcgg accgagcggc
     4501 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     4561 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     4621 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     4681 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     4741 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4801 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4861 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4921 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4981 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5041 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc
     5101 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     5161 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     5221 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     5281 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     5341 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     5401 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5461 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5521 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5581 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5641 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5701 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5761 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     5821 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5881 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5941 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     6001 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     6061 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     6121 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6181 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6241 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6301 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6361 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6421 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6481 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6541 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6601 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6661 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6721 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6781 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6841 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6901 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6961 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7021 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7081 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7141 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7201 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
