LOCUS       .                       5672 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    1367..1400
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /label="lox2272-JTZ17"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     CDS             2380..2445
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /codon_start=1
                     /transl_table=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     promoter        3887..3991
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     repeat_region   2935..3075
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     terminator      2461..2892
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     rep_origin      3150..3605
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     rep_origin      5023..5611
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     CDS             3992..4852
                     /gene="bla"
                     /product="β-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     CDS             1493..2373
                     /gene="ARHGAP5"
                     /product="rho GTPase-activating protein 5 isoform a"
                     /translation="GIDNPAITSDQELDDKKMKKKTHKVKEDKKQKKKTKNFNPPTRRN
                     WESNYFGMPLQDLVTAEKPIPLFVEKCVEFIEDTGLCTEGLYRVSGNKTDQDNIQKQFD
                     QDHNINLVSMEVTVNAVAGALKAFFADLPDPLIPYSLHPELLEAAKIPDKTERLHALKE
                     IVKKFHPVNYDVFRYVITHLNRVSQQHKINLMTADNLSICFWPTLMRPDFENREFLSTT
                     KIHQSVVETFIQQCQFFFYNGEIVETTNIVAPPPPSNPGQLVEPMVPLQLPPPLQPQLI
                     QPQLQTDPLGII"
                     /note="isoform a is encoded by transcript variant 1; growth
                     factor independent 2; rho GTPase-activating protein 5; p105
                     RhoGAP; p100 RasGAP-associated p105 protein; rho-type
                     GTPase-activating protein 5"
                     /note="color: #993366"
                     /db_xref="{'GI': '71834863', 'CCDS': 'CCDS32062.1',
                     'GeneID': '394', 'HGNC': 'HGNC:675', 'MIM': '602680'}"
                     /gene_synonym="GFI2; p190-B; p190BRhoGAP; RhoGAP5"
                     /protein_id="NP_001025226.1"
                     /codon_start=1
                     /transl_table=1
                     /label="ARHGAP5"
     CDS             288..1318
                     /product="site-specific recombinase"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /protein_id=""
                     /codon_start=1
                     /transl_table=1
                     /label="Cre"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtggtatt
     1501 gataatcctg caatcacttc tgaccaggag ttagatgata agaagatgaa gaagaaaacc
     1561 cacaaagtga aagaagataa aaagcagaaa aagaaaacta agaacttcaa tccaccaaca
     1621 cgtagaaatt gggaaagtaa ttactttggg atgcccctcc aggatctggt tacagctgag
     1681 aagcccatac cactatttgt tgagaaatgt gtggaattta ttgaagatac agggttatgt
     1741 accgaaggac tctaccgtgt cagcgggaat aaaactgacc aagacaatat tcaaaagcag
     1801 tttgatcaag atcataatat caatctagtg tcaatggaag taacagtaaa tgctgtagct
     1861 ggagccctta aagctttctt tgcagatctg ccagatcctt taattccata ttctcttcat
     1921 ccagaactat tggaagcagc aaaaatcccg gataaaacag aacgtcttca tgccttgaaa
     1981 gaaattgtta agaaatttca tcctgtaaac tatgatgtat tcagatacgt gataacacat
     2041 ctaaacaggg ttagtcagca acataaaatc aacctaatga cagcagacaa cttatccatc
     2101 tgtttttggc caaccttgat gagacctgat tttgaaaatc gagagtttct gtctactact
     2161 aagattcatc aatctgttgt tgaaacattc attcagcagt gtcagttttt cttttacaat
     2221 ggagaaattg tagaaacgac aaacattgtg gctcctccac caccttcaaa cccaggacag
     2281 ttggtggaac caatggtgcc acttcagttg ccgccaccat tgcaacctca gctgatacaa
     2341 ccacaattac aaacggatcc tcttggtatt atatgtacag actacaaaga ccatgacggt
     2401 gattataaag atcatgacat cgactacaag gatgacgatg acaagtaaga attctgatga
     2461 ctcgagataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     2521 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     2581 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttagtt cttgccacgg
     2641 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     2701 acaattccgt ggtgtttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatct
     2761 agctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     2821 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg
     2881 gaggtttttt aaagatctgg agaccggtgt ggcacgtgcg gaccgagcgg ccgcaggaac
     2941 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
     3001 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
     3061 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     3121 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
     3181 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     3241 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3301 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3361 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     3421 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     3481 ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     3541 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     3601 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     3661 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     3721 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     3781 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     3841 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3901 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3961 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4021 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4081 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4141 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4201 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4261 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4321 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4381 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4441 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4501 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     4561 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4621 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4681 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     4741 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4801 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     4861 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4921 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4981 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5041 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5101 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5161 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5221 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5281 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5341 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5401 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5461 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5521 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5581 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5641 cctggccttt tgctggcctt ttgctcacat gt
//
