LOCUS       .                       5576 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             727..753
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /codon_start=1
                     /transl_table=1
                     /label="HA"
                     /note="color: #cc99b2"
     misc_feature    2761..2794
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     intron          2717..2757
                     /label="Splicing Donor"
                     /note="color: noColor"
     promoter        3791..3895
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   2839..2979
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     rep_origin      3054..3509
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      4927..5515
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     CDS             3896..4756
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /product="β-lactamase"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     CDS             760..2716
                     /protein_id="NP_853530.2"
                     /note="isoform a is encoded by transcript variant 1; golgi
                     integral membrane protein 6; protein CASP; cut homolog;
                     putative protein product of Nbla10317; homeobox protein
                     cux-1; CCAAT displacement protein; CUX1 gene Alternatively
                     Spliced Product; mutant CUX1; CDP/Cux p200"
                     /note="color: #993366"
                     /translation="LCVAGARLKRELDATATVLANRQDESEQSRKRLIEQSREFKKNTP
                     EDLRKQVAPLLKSFQGEIDALSKRSKEAEAAFLNVYKRLIDVPDPVPALDLGQQLQLKV
                     QRLHDIETENQKLRETLEEYNKEFAEVKNQEVTIKALKEKIREYEQTLKNQAETIALEK
                     EQKLQNDFAEKERKLQETQMSTTSKLEEAEHKVQSLQTALEKTRTELFDLKTKYDEETT
                     AKADEIEMIMTDLERANQRAEVAQREAETLREQLSSANHSLQLASQIQKAPDVEQAIEV
                     LTRSSLEVELAAKEREIAQLVEDVQRLQASLTKLRENSASQISQLEQQLSAKNSTLKQL
                     EEKLKGQADYEEVKKELNILKSMEFAPSEGAGTQDAAKPLEVLLLEKNRSLQSENAALR
                     ISNSDLSGSARRKGKDQPESRRPGSLPAPPPSQLPRNPGEQASNTNGTHQFSPAGLSQD
                     FFSSSLASPSLPLASTGKFALNSLLQRQLMQSFYSKAMQEAGSTSMIFSTGPYSTNSIS
                     SQSPLQQSPDVNGMAPSPSQSESAGSVSEGEEMDTAEIARQVKEQLIKHNIGQRIFGHY
                     VLGLSQGSVSEILARPKPWNKLTVRGKEPFHKMKQFLSDEQNILALRSIQGRQRENPGQ
                     SLNRLFQEVPKRRNGSE"
                     /gene="CUX1"
                     /db_xref="{'GI': '148277064', 'CCDS': 'CCDS5721.1',
                     'GeneID': '1523', 'HGNC': 'HGNC:2557', 'MIM': '116896'}"
                     /product="Homeobox protein cut-like 1 isoform a"
                     /codon_start=1
                     /gene_synonym="CASP; CDP; CDP/Cut; CDP1; Clox; COY1; CUTL1;
                     CUX; Cux/CDP; GDDI; GOLIM6; Nbla10317; p100; p110; p200;
                     p75"
                     /transl_table=1
                     /label="CUX1"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatctt tgtgcgtagc cggagccagg
      781 ttgaagagag aactcgatgc caccgcaacg gtattggcga accggcagga tgaaagtgag
      841 cagtccagaa agcggcttat cgaacagagc cgggagttca agaagaacac tccagaggat
      901 ttgcgcaagc aggtagcgcc gctgctgaag agtttccaag gagagattga tgcactgagt
      961 aaaagaagca aggaagctga agcagctttc ttgaatgtct acaaaagatt gattgacgtc
     1021 ccagatcccg taccagcttt ggatctcgga cagcaactcc agctcaaagt gcagcgcctg
     1081 cacgatattg aaacagagaa ccagaaactt agggaaactc tggaagaata caacaaggaa
     1141 tttgctgaag tgaaaaatca agaggttacg ataaaagcac ttaaagagaa aatccgagaa
     1201 tatgaacaga cactgaagaa ccaagccgaa accatagctc ttgagaagga acagaagtta
     1261 cagaatgact ttgcagaaaa ggagagaaag ctgcaggaga cacagatgtc caccacctca
     1321 aagctggagg aagctgagca taaggttcag agcctacaaa cagccctgga aaaaactcga
     1381 acagaattat ttgacctgaa aaccaaatac gatgaagaaa ctactgcaaa ggccgacgag
     1441 attgaaatga tcatgacgga ccttgaaagg gcaaaccaga gggcagaggt ggctcagaga
     1501 gaggcggaga ccttaaggga acagctctca tcggccaatc actccctcca gctggcctca
     1561 cagatccaga aggcaccaga cgtggagcag gccatagagg tgctgacccg ctccagccta
     1621 gaagttgagt tggccgccaa ggagcgggag atcgcacagc tggtggagga cgtgcagaga
     1681 ctccaggcca gcctcaccaa gctgcgggag aattcggcca gccagatctc acagcttgag
     1741 cagcagctga gcgccaaaaa cagcacactc aaacaactgg aagaaaaact caaaggccag
     1801 gctgactatg aagaggtgaa gaaagagctg aacattctga agtccatgga gtttgcaccg
     1861 tccgagggcg ctgggacaca ggatgcggcc aagcccctgg aggtgctgtt gctggagaag
     1921 aaccgctcgc tgcagtccga gaacgccgcg ctgcgcatct ccaacagcga cctgagcggg
     1981 tcagccagga ggaaagggaa agaccagcct gaaagtcggc gcccgggatc tttgccggcc
     2041 ccccctcctt ctcagttgcc ccgcaacccg ggggagcagg cttccaatac taatggtaca
     2101 caccagttct caccagcggg gttaagtcaa gactttttca gctcatccct ggcaagcccc
     2161 agcctacccc tggcttctac aggaaaattt gcactaaact ctcttctcca gcggcagcta
     2221 atgcagtcct tctactccaa ggctatgcag gaagccggaa gcacaagcat gattttttca
     2281 acaggtccat acagcacaaa ctccatatct tcccaaagtc cattacaaca aagcccagat
     2341 gtcaatggca tggccccatc ccccagccag tcagaaagtg ctgggagcgt ctccgagggc
     2401 gaggagatgg acactgcaga aatcgcccgg caggtcaaag agcagctgat taagcacaat
     2461 atcggacaac gtattttcgg acattatgtg ttgggactgt ctcaagggtc cgtgagcgag
     2521 attctggccc ggcccaagcc atggaataaa ctgactgttc gtggcaagga gccatttcac
     2581 aagatgaaac agttcctctc cgatgagcag aacatcctgg ccctccgtag catccaaggc
     2641 agacaaagag agaatccagg ccagagcctg aacagactat ttcaggaagt accgaaacga
     2701 agaaatgggt ctgaaggtaa gtatcaaggt tacaagacag gtttaaggag accaataagc
     2761 tattattcgt ataggatact ttatacgaag ttattaagtt atggagaccg gtgtggcacg
     2821 tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     2881 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
     2941 cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc
     3001 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc
     3061 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     3121 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     3181 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     3241 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     3301 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     3361 gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat
     3421 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     3481 ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga
     3541 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     3601 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     3661 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     3721 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     3781 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     3841 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     3901 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     3961 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     4021 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     4081 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     4141 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     4201 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     4261 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     4321 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     4381 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     4441 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     4501 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     4561 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     4621 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     4681 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     4741 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     4801 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     4861 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4921 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4981 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     5041 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     5101 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     5161 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     5221 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     5281 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     5341 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     5401 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     5461 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     5521 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//
