LOCUS       .                       7334 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    1367..1400
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /bound_moiety="Cre recombinase"
                     /label="lox2272-JTZ17"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     CDS             4054..4119
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     promoter        5549..5653
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     repeat_region   4597..4737
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     terminator      4123..4554
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     rep_origin      4812..5267
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     rep_origin      6685..7273
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     CDS             5654..6514
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /codon_start=1
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     CDS             288..1318
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /codon_start=1
                     /product="site-specific recombinase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /label="Cre"
     CDS             1493..4047
                     /gene="CUX1"
                     /gene_synonym="CASP; CDP; CDP/Cut; CDP1; Clox; COY1; CUTL1;
                     CUX; Cux/CDP; GDDI; GOLIM6; Nbla10317; p100; p110; p200;
                     p75"
                     /note="isoform a is encoded by transcript variant 1; golgi
                     integral membrane protein 6; protein CASP; cut homolog;
                     putative protein product of Nbla10317; homeobox protein
                     cux-1; CCAAT displacement protein; CUX1 gene Alternatively
                     Spliced Product; mutant CUX1; CDP/Cux p200"
                     /note="color: #993366"
                     /codon_start=3
                     /product="Homeobox protein cut-like 1 isoform a"
                     /protein_id="NP_853530.2"
                     /db_xref="{'GI': '148277064', 'CCDS': 'CCDS5721.1',
                     'GeneID': '1523', 'HGNC': 'HGNC:2557', 'MIM': '116896'}"
                     /transl_table=1
                     /translation="NITTRIRASETGSDEAIKSILEQAKRELQVQKTAEPAQPSSASGS
                     GNSDDAIRSILQQARREMEAQQAALDPALKQAPLSQSDITILTPKLLSTSPMPTVSSYP
                     PLAISLKKPSAAPEAGASALPNPPALKKEAQDAPGLDPQGAADCAQGVLRQVKNEVGRS
                     GAWKDHWWSAVQPERRNAASSEEAKAEETGGGKEKGSGGSGGGSQPRAERSQLQGPSSS
                     EYWKEWPSAESPYSQSSELSLTGASRSETPQNSPLPSSPIVPMSKPTKPSVPPLTPEQY
                     EVYMYQEVDTIELTRQVKEKLAKNGICQRIFGEKVLGLSQGSVSDMLSRPKPWSKLTQK
                     GREPFIRMQLWLNGELGQGVLPVQGQQQGPVLHSVTSLQDPLQQGCVSSESTPKTSASC
                     SPAPESPMSSSESVKSLTELVQQPCPPIEASKDSKPPEPSDPPASDSQPTTPLPLSGHS
                     ALSIQELVAMSPELDTYGITKRVKEVLTDNNLGQRLFGETILGLTQGSVSDLLARPKPW
                     HKLSLKGREPFVRMQLWLNDPNNVEKLMDMKRMEKKAYMKRRHSSVSDSQPCEPPSVGT
                     EYSQGASPQPQHQLKKPRVVLAPEEKEALKRAYQQKPYPSPKTIEDLATQLNLKTSTVI
                     NWFHNYRSRIRRELFIEEIQAGSQGQAGASDSPSARSGRAAPSSEGDSCDGVEATEGPG
                     SADTEEPKSQGEAEREEVPRPAEQTEPPPSGTPGPDDARDDDHEGGPVEGPGPLPSPAS
                     ATATAAPAAPEDAATSAAAAPGEGPAAPSSAPPPSNSSSSSAPRRPSSLQSLFGLPEAA
                     GARDSRDNPLRKKKAANLNSIIHRLEKAASREEPIEWEF"
                     /label="CUX1"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtaacatc
     1501 accacccgga tccgagcctc ggagactggc tctgatgaag ccatcaagtc catcctagag
     1561 caagccaaga gggagctcca agtgcagaaa actgcagagc cggcccagcc ttcctccgca
     1621 tccggcagcg ggaactctga tgacgccatc cgctccatcc tgcagcaagc ccgccgggag
     1681 atggaggccc agcaggctgc cctcgaccct gccttaaagc aggcaccact gtcccagagt
     1741 gacatcacca tcctcacccc caagcttctg tccacctcgc ccatgcccac cgtgtccagc
     1801 tacccacctc tcgccatctc cctgaagaag ccctccgcag ctcctgaggc cggtgcctct
     1861 gctctgccga accccccggc cctcaaaaag gaggcccagg acgcccccgg gctggacccc
     1921 cagggagcag ccgattgtgc acaaggggtc ctgagacagg tgaaaaatga ggtgggccgc
     1981 agcggtgcct ggaaggacca ctggtggagc gcggtgcagc cggagagaag aaatgccgcc
     2041 tcctccgagg aggccaaggc cgaagaaacg ggcggcggga aagagaaggg cagcggtggc
     2101 agcggaggtg gcagccagcc tcgggccgag cgcagtcagc tccagggacc ctcgtcgtca
     2161 gagtactgga aggagtggcc cagcgctgag tccccatact cccagagctc agagctgagt
     2221 ctgaccgggg ccagccgcag cgagacacca cagaacagcc ccctgccatc ctccccgatc
     2281 gtgcccatgt ccaagcccac caagccctcg gtccccccgc tgacccccga gcagtacgag
     2341 gtctacatgt accaggaggt ggacaccatc gagctcaccc ggcaggttaa ggaaaagctg
     2401 gccaagaacg gcatctgcca gagaatcttc ggggagaagg tgctgggcct gtcccagggc
     2461 agcgtcagcg acatgctgtc ccgaccgaag ccatggagca agctgacgca gaaaggccga
     2521 gaacccttca tccggatgca gctctggctg aacggcgagc taggccaggg tgttctaccc
     2581 gtccagggcc agcagcaagg gccagtcctc cactccgtga catcgctcca ggacccgctg
     2641 cagcagggct gtgtgagctc agaaagcact ccaaagacct ccgccagctg cagccctgcc
     2701 cctgagtccc cgatgagttc cagtgagtcg gtgaagagcc tgaccgagct ggtccagcag
     2761 ccctgtcccc ccatcgaggc gagcaaggac agcaagccac cagagcccag tgacccgcca
     2821 gcatccgact cccagcccac aaccccgctg cctctctccg gacactcggc cctcagcatc
     2881 caagaattag tagccatgtc cccggagctg gacacctacg gcataaccaa gcgggtgaag
     2941 gaggtgctga cggacaacaa cctcggccag cgcttatttg gggagaccat cttagggctc
     3001 acccaaggct ctgtctctga cctccttgcc cgccccaaac cctggcataa gctcagtctg
     3061 aaaggacgag agcccttcgt ccggatgcag ctgtggctga acgaccccaa caatgtggag
     3121 aagctgatgg acatgaaacg gatggagaag aaagcctaca tgaagcggcg gcacagctca
     3181 gtcagtgaca gccagccctg cgaaccgccc tctgtcggca ccgagtacag ccagggcgcc
     3241 agcccccagc cccagcacca gctgaagaaa ccccgggtgg tgctggctcc ggaggagaag
     3301 gaggcgctga aacgagcgta tcagcaaaag ccatacccgt caccaaaaac catcgaagac
     3361 ctcgccaccc agctcaacct gaaaaccagc accgtcatca actggttcca caactacagg
     3421 tctcggatcc gcagagaact gttcattgag gaaattcagg ccgggagtca gggccaggcg
     3481 ggcgccagcg actcaccctc ggcccgcagc ggccgggcgg cgcccagctc ggagggcgac
     3541 agctgcgacg gcgtggaggc cactgagggc ccaggcagcg ccgacaccga ggagcccaag
     3601 tctcagggag aggccgagcg ggaggaggtg ccgcggccgg cggagcagac ggagccgccg
     3661 ccctcgggga ccccgggccc ggacgacgcc cgcgacgacg accacgaggg aggccccgtg
     3721 gaaggcccgg ggcccctgcc cagccccgcc tccgcgaccg ccaccgccgc gcccgcggcc
     3781 cccgaggacg ccgctacctc agccgccgcc gcgccggggg agggccccgc ggccccgagc
     3841 tccgcgccgc cgcccagcaa cagcagcagc agcagcgccc cccgcaggcc cagctcgctg
     3901 cagagccttt tcggcctccc cgaggccgcg ggcgcccggg actcgcgcga caaccccctg
     3961 cgcaagaaga aggccgcgaa cttgaacagc atcatccacc gcctggagaa ggccgccagc
     4021 cgggaggaac ctatcgaatg ggagttctgt acagactaca aagaccatga cggtgattat
     4081 aaagatcatg acatcgacta caaggatgac gatgacaagt aactcgagat aatcaacctc
     4141 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     4201 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     4261 ttttctcctc cttgtataaa tcctggttag ttcttgccac ggcggaactc atcgccgcct
     4321 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttta
     4381 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat ctagctttat ttgtgaaatt
     4441 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     4501 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagatct
     4561 ggagaccggt gtggcacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
     4621 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     4681 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
     4741 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
     4801 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4861 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4921 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4981 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     5041 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5101 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc
     5161 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5221 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
     5281 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     5341 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     5401 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     5461 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     5521 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5581 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5641 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5701 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5761 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5821 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5881 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     5941 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6001 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6061 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6121 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6181 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6241 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6301 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6361 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6421 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6481 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6541 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6601 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6661 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6721 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6781 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     6841 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6901 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6961 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7021 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7081 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7141 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7201 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7261 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7321 ttttgctcac atgt
//
