LOCUS       .                       6305 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             760..3445
                     /protein_id="NP_001382820.1"
                     /note="isoform 8 is encoded by transcript variant 8;
                     CLIP-associating protein 1; multiple asters 1; protein
                     Orbit homolog 1; multiple asters homolog 1"
                     /note="color: #993366"
                     /translation="MEPRMESCLAQVLQKDVGKRLQVGQELIDYFSDKQKSADLEHDQT
                     MLDKLVDGLATSWVNSSNYKVVLLGMDILSALVTRLQDRFKAQIGTVLPSLIDRLGDAK
                     DSVREQDQTLLLKIMDQAANPQYVWDRMLGGFKHKNFRTREGICLCLIATLNASGAQTL
                     TLSKIVPHICNLLGDPNSQVRDAAINSLVEIYRHVGERVRADLSKKGLPQSRLNVIFTK
                     FDEVQKSGNMIQSANDKNFDDEDSVDGNRPSSASSTSSKAPPSSRRNVGMGTTRRLGSS
                     TLGSKSSAAKEGAGAVDEEDFIKAFDDVPVVQIYSSRDLEESINKIREILSDDKHDWEQ
                     RVNALKKIRSLLLAGAAEYDNFFQHLRLLDGAFKLSAKDLRSQVVREACITLGHLSSVL
                     GNKFDHGAEAIMPTIFNLIPNSAKIMATSGVVAVRLIIRHTHIPRLIPVITSNCTSKSV
                     AVRRRCFEFLDLLLQEWQTHSLERHISVLAETIKKGIHDADSEARIEARKCYWGFHSHF
                     SREAEHLYHTLESSYQKALQSHLKNSDSIVSLPQSDRSSSSSQESLNRPLSAKRSPTGS
                     TTSRASTVSTKSVSTTGSLQRSRSDIDVNAAASAKSKVSSSSGTTPFSSAAALPPGSYA
                     SLGRIRTRRQSSGSATNVASTPDNRGRSRAKVVSQSQRSRSANPAGAGSRSSSPGKLLG
                     SGYGGLTGGSSRGPPVTPSSEKRSKIPRSQGCSRETSPNRIGLARSSRIPRPSMSQGCS
                     RDTSRESSRDTSPARGFPPLASRRHSRSTSALSTAESVGQSDRFGLGQPGRIPGSVNAM
                     RVLSTSTDLEAAVADALKKPVRRRYEPYGMYSDDDANSDASSVCSERSYGSRNGGIPHY
                     LRQTEDVAEVLNHCASSNWSERKE"
                     /gene="CLASP1"
                     /db_xref="{'GI': '2085776125', 'CCDS': 'CCDS92853.1',
                     'GeneID': '23332', 'HGNC': 'HGNC:17088', 'MIM': '605852'}"
                     /product="CLIP-associating protein 1 isoform 8"
                     /codon_start=1
                     /gene_synonym="MAST1"
                     /transl_table=1
                     /label="CLASP1"
     CDS             4625..5485
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /product="β-lactamase"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     rep_origin      5656..6244
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      3783..4238
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   3568..3708
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     promoter        4520..4624
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          3446..3486
                     /label="Splicing Donor"
                     /note="color: noColor"
     misc_feature    3490..3523
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     CDS             727..753
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /codon_start=1
                     /transl_table=1
                     /label="HA"
                     /note="color: #cc99b2"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatcta tggagcctcg catggagtcc
      781 tgcctggcgc aggtgttgca gaaggatgtg gggaaacgat tgcaggttgg ccaagaactg
      841 atagactatt tctcagacaa acagaagtct gctgaccttg agcatgacca gaccatgtta
      901 gataaacttg tggatggact tgctacctct tgggtgaact ctagcaatta caaggtggtt
      961 ctgctgggca tggacatcct gtccgccctg gtgacccggc tgcaggatcg gttcaaggcg
     1021 cagatcggca cagtgctgcc aagtctaata gacagactag gagatgctaa agactctgtg
     1081 agggagcagg accaaactct gctgctaaag atcatggatc aagctgctaa tccccagtac
     1141 gtatgggaca gaatgcttgg aggcttcaaa cacaagaatt tccgtactcg agaaggcatc
     1201 tgtctctgcc ttatagcaac actcaatgcc tctggagcac agactttaac actaagcaag
     1261 attgtgccac atatatgcaa cttacttgga gatccaaaca gccaggttcg agatgcagca
     1321 ataaacagct tagtggaaat ttacagacat gtaggagaac gtgtgagggc agatctcagt
     1381 aaaaaaggat tgccacagtc ccggttgaat gtaattttta caaaatttga tgaagtccag
     1441 aaatctggaa acatgataca atctgcaaat gataaaaatt tcgacgatga agattctgtg
     1501 gatggtaaca gaccttcctc tgctagttct acatcatcca aggctccacc aagttctcgg
     1561 agaaacgttg gaatgggaac cacccgccgg cttggttcat ccacccttgg atccaagtct
     1621 tcagctgcaa aagaaggagc tggtgctgtt gatgaagagg attttattaa agcatttgat
     1681 gatgtacctg tagtacagat ttattccagc cgagaccttg aggaatccat aaacaaaatt
     1741 agggaaatat tatctgatga caagcatgat tgggagcaga gagtaaatgc tctaaaaaag
     1801 attagatctt tacttttggc tggtgctgct gagtatgata acttctttca acatttgcgt
     1861 cttttggatg gagcctttaa actctctgct aaggacctgc ggtctcaggt agtgcgggag
     1921 gcttgtatca cgttggggca tctgtcatca gttctgggga ataagtttga ccatggagct
     1981 gaagccatta tgccaactat ctttaattta attccaaaca gtgccaaaat tatggccaca
     2041 tctggtgttg tagctgttag gttaattatt cggcacacac acatccctag gttaatacct
     2101 gtcataacaa gcaactgtac ctctaagtct gtcgcagtta gaaggcgctg ttttgaattt
     2161 ttagatttgc ttttacaaga atggcagaca cattcactag aacgacacat atcagtatta
     2221 gctgaaacaa taaagaaggg aatacatgat gctgattccg aagcaagaat agaagccaga
     2281 aaatgttact ggggtttcca cagtcacttc agcagagaag cagagcactt gtaccacacc
     2341 ttggagtcct cctaccagaa agccctgcag tcccacctga agaactcaga cagcatagtg
     2401 tctctgcctc agtcagaccg ctcatcttcc agctctcaag agagtctaaa tcgtccgctg
     2461 tctgccaaaa gaagtcctac tggaagtacc acatctagag cttctacagt tagtaccaaa
     2521 tctgtgtcaa cgactgggtc cctccagcga tctcgaagtg atattgatgt gaacgcagca
     2581 gccagtgcca aatccaaagt ctcctcatct tcgggcacga cgcctttcag ctctgcagca
     2641 gctttgcctc cagggtcata cgcatcctta ggtcggatcc gcacaagacg gcaaagctct
     2701 gggagtgcca ccaacgtcgc ctctacacct gataaccggg gccgcagtcg cgctaaagtg
     2761 gtttcacagt cccagcgatc cagatctgct aatcctgctg gtgctggcag ccggtcaagt
     2821 tccccaggaa aattgttggg aagtggttat ggtggactta ctgggggctc ctcacgaggc
     2881 ccacctgtga caccgtcttc agaaaagcga agcaagattc ccaggagcca gggatgtagc
     2941 cgggaaacaa gtccaaaccg aataggatta gcacggagca gccgtatccc tcgacccagc
     3001 atgagtcagg ggtgcagccg cgataccagc cgtgagagca gccgagatac aagccctgct
     3061 cggggctttc ctccacttgc ctctcgacgt cattccaggt ccactagtgc tctctccact
     3121 gctgaatctg ttgggcagtc agatcggttt gggcttggcc agccaggaag aatacctggt
     3181 tctgtgaatg ccatgagagt tctgagcaca agtacagatc ttgaagctgc tgttgctgat
     3241 gctttgaaga agcctgtgag gaggagatat gagccgtatg ggatgtattc tgacgatgat
     3301 gccaacagtg atgcctcaag tgtttgctct gagcgctcat atggctccag gaatggtggc
     3361 attccccatt atctgcggca gactgaggat gtagcagaag ttctcaacca ctgtgctagt
     3421 tcaaactggt cagaaaggaa agaaggtaag tatcaaggtt acaagacagg tttaaggaga
     3481 ccaataagct attattcgta taggatactt tatacgaagt tattaagtta tggagaccgg
     3541 tgtggcacgt gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct
     3601 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
     3661 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc
     3721 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
     3781 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3841 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     3901 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     3961 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
     4021 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     4081 tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt
     4141 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     4201 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
     4261 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     4321 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     4381 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     4441 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     4501 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4561 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4621 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     4681 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     4741 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     4801 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4861 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4921 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4981 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5041 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5101 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5161 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5221 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5281 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5341 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5401 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5461 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     5521 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5581 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5641 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5701 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5761 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     5821 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5881 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5941 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6001 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6061 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6121 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6181 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6241 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6301 catgt
//
