LOCUS       .                       6208 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             727..753
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /codon_start=1
                     /transl_table=1
                     /label="HA"
                     /note="color: #cc99b2"
     misc_feature    3393..3426
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     intron          3349..3389
                     /label="Splicing Donor"
                     /note="color: noColor"
     promoter        4423..4527
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   3471..3611
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     rep_origin      3686..4141
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      5559..6147
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     CDS             4528..5388
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /product="β-lactamase"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     CDS             760..3348
                     /protein_id="NP_055789.1"
                     /note="DIP2 homolog C; DIP2 disco-interacting protein 2
                     homolog C"
                     /note="color: #993366"
                     /translation="ADRSLEGMALPLEVRARLAELELELSEGDITQKGYEKKRSKLIGA
                     YLPQPPRVDQALPQERRAPVTPSSASRYHRRRSSGSRDERYRSDVHTEAVQAALAKHKE
                     RKMAVPMPSKRRSLVVQTSMDAYTPPDTSSGSEDEGSVQGDSQGTPTSSQGSINMEHWI
                     SQAIHGSTTSTTSSSSTQSGGSGAAHRLADVMAQTHIENHSAPPDVTTYTSEHSIQVER
                     PQGSTGSRTAPKYGNAELMETGDGVPVSSRVSAKIQQLVNTLKRPKRPPLREFFVDDFE
                     ELLEVQQPDPNQPKPEGAQMLAMRGEQLGVVTNWPPSLEAALQRWGTISPKAPCLTTMD
                     TNGKPLYILTYGKLWTRSMKVAYSILHKLGTKQEPMVRPGDRVALVFPNNDPAAFMAAF
                     YGCLLAEVVPVPIEVPLTRKDAGSQQIGFLLGSCGVTVALTSDACHKGLPKSPTGEIPQ
                     FKGWPKLLWFVTESKHLSKPPRDWFPHIKDANNDTAYIEYKTCKDGSVLGVTVTRTALL
                     THCQALTQACGYTEAETIVNVLDFKKDVGLWHGILTSVMNMMHVISIPYSLMKVNPLSW
                     IQKVCQYKAKVACVKSRDMHWALVAHRDQRDINLSSLRMLIVADGANPWSISSCDAFLN
                     VFQSKGLRQEVICPCASSPEALTVAIRRPTDDSNQPPGRGVLSMHGLTYGVIRVDSEEK
                     LSVLTVQDVGLVMPGAIMCSVKPDGVPQLCRTDEIGELCVCAVATGTSYYGLSGMTKNT
                     FEVFPMTSSGAPISEYPFIRTGLLGFVGPGGLVFVVGKMDGLMVVSGRRHNADDIVATA
                     LAVEPMKFVYRGRIAVFSVTVLHDERIVIVAEQRPDSTEEDSFQWMSRVLQ"
                     /gene="DIP2C"
                     /db_xref="{'GI': '44888818', 'CCDS': 'CCDS7054.1',
                     'GeneID': '22982', 'HGNC': 'HGNC:29150', 'MIM': '611380'}"
                     /product="disco-interacting protein 2 homolog C"
                     /codon_start=1
                     /gene_synonym="KIAA0934"
                     /transl_table=1
                     /label="DIP2C"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatctg cggaccgcag cctggagggc
      781 atggcgctgc ccctggaggt gcgggcgcgc ctggccgagc tggagctgga gctgtcggaa
      841 ggtgacatca cacaaaaagg atatgaaaag aagaggtcaa agttaattgg agcctacctt
      901 ccgcagcctc cgagggtgga ccaagctttg ccgcaagaac gccgggctcc tgtcactcct
      961 tcctccgcct ctcgctacca ccgccgacgg tcttcagggt cacgagatga gcgctatcgg
     1021 tcagacgtcc acacggaagc tgtccaggcg gctctggcca aacacaaaga gcggaagatg
     1081 gcagtgccta tgccttccaa acgcaggtcc ctggtcgtgc agacctcgat ggacgcctac
     1141 acccctccag atacctcttc tggctcagaa gatgaaggct cagtgcaggg ggactcccag
     1201 ggcaccccca cctccagcca gggcagcatc aatatggagc actggatcag ccaggccatc
     1261 cacggctcca ccacgtccac cacctcctcg tcctctacgc agagcggggg cagcggggct
     1321 gcccacaggc tggcggacgt catggctcag acccacatag aaaatcattc tgcacctcct
     1381 gacgtaacca cgtacacctc agagcactcg atacaggtgg agagaccgca gggttccacg
     1441 gggtcccgga cagcgcccaa gtacggcaac gccgagctca tggagaccgg ggatggagta
     1501 ccagtaagta gccgggtgtc agcaaaaatc cagcagcttg tcaataccct caaacgaccg
     1561 aaacgaccac ctttacgaga attctttgtc gatgactttg aagaattatt agaagttcaa
     1621 caaccggatc cgaaccaacc aaagccggag ggggcccaga tgctggccat gcgcggagag
     1681 cagctgggcg tggtcacgaa ctggccgccg tcgctggagg ccgcactgca gaggtggggc
     1741 accatctcgc ccaaggcgcc ctgcctgacc accatggaca ccaacgggaa gcccctctac
     1801 atcctcactt acggcaagct gtggacaaga agtatgaagg tcgcttacag cattctacac
     1861 aaattaggca caaagcagga acccatggtc cggcctggag atagggtggc actggtgttc
     1921 cccaacaatg atccggctgc cttcatggcg gctttctacg gctgcctgct ggccgaggtg
     1981 gtccccgtgc ccatcgaggt gccactcacc aggaaggacg cagggagcca gcagataggt
     2041 ttcttgcttg gaagctgtgg agttactgta gccttgacta gtgacgcctg ccataaagga
     2101 cttccaaaaa gcccaacggg agagatccca cagtttaaag gttggccaaa gctgctgtgg
     2161 tttgtcacag agtctaaaca tctctccaaa ccgccccgag actggttccc acacattaaa
     2221 gatgccaata acgacactgc gtatattgag tacaagacgt gtaaggatgg cagtgtgctg
     2281 ggtgtgacgg tgacgaggac tgcgctgctg acacactgcc aggccctgac gcaggcgtgt
     2341 ggctacacgg aagctgaaac cattgtgaat gtgctggatt tcaagaagga cgtcgggctc
     2401 tggcatggca tcctgacaag cgtcatgaac atgatgcatg tgatcagcat cccgtactcg
     2461 ctgatgaagg tgaaccctct ctcctggatc cagaaggtct gccagtacaa agcaaaagtg
     2521 gcgtgtgtga aatcgaggga tatgcattgg gcattagtag cacacagaga tcagagagac
     2581 atcaacctct cctctctgcg aatgctgata gtggcggacg gcgcgaaccc ctggtctatt
     2641 tcttcttgcg atgcatttct caatgtcttc caaagtaaag gccttcgaca ggaggtcatc
     2701 tgtccttgtg ccagctcgcc agaggccctc actgtggcca tccggaggcc cacggatgac
     2761 agtaaccagc ccccgggccg gggtgtcctc tccatgcatg gactgaccta tggggtcatt
     2821 cgtgtggact cggaagagaa gctgtccgtg ctcaccgtgc aggatgtcgg cctcgtgatg
     2881 cctggagcca tcatgtgttc agtgaagcca gacggggttc ctcagctgtg cagaacggat
     2941 gagatcgggg agctgtgtgt gtgtgcagtt gcgacgggca cgtcctacta tggcctctct
     3001 ggcatgacca agaacacctt tgaggtgttt cccatgacaa gctccggggc tccgatcagt
     3061 gaatacccat tcataaggac aggcttgctg gggttcgtgg gtcccggagg cctcgtcttc
     3121 gtggtgggca agatggatgg cctcatggtg gtcagcgggc gcaggcacaa cgccgacgac
     3181 atcgtggcca ctgcgctggc cgtagaaccc atgaagtttg tctaccgggg aaggatagcc
     3241 gtgttctcgg tgaccgtgct gcacgacgag aggatcgtga tcgtggctga gcagaggcct
     3301 gactccacgg aagaggacag tttccagtgg atgagccgtg tgctgcaggt aagtatcaag
     3361 gttacaagac aggtttaagg agaccaataa gctattattc gtataggata ctttatacga
     3421 agttattaag ttatggagac cggtgtggca cgtgcggacc gagcggccgc aggaacccct
     3481 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
     3541 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
     3601 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     3661 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3721 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3781 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3841 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     3901 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     3961 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4021 atctcgggct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4081 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     4141 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     4201 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     4261 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     4321 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     4381 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4441 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4501 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4561 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4621 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4681 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     4741 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     4801 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     4861 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     4921 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     4981 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5041 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     5101 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5161 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5221 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5281 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5341 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     5401 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5461 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5521 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5581 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5641 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5701 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5761 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5821 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5881 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5941 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6001 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6061 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6121 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6181 gccttttgct ggccttttgc tcacatgt
//
