LOCUS       .                       6867 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1493..3568
                     /gene="DIP2C"
                     /product="disco-interacting protein 2 homolog C"
                     /translation="AIDSIHQVGVYCLALVPANTLPKTPLGGIHLSETKQLFLEGSLHP
                     CNVLMCPHTCVTNLPKPRQKQPEIGPASVMVGNLVSGKRIAQASGRDLGQIEDNDQARK
                     FLFLSEVLQWRAQTTPDHILYTLLNCRGAIANSLTCVQLHKRAEKIAVMLMERGHLQDG
                     DHVALVYPPGIDLIAAFYGCLYAGCVPITVRPPHPQNIATTLPTVKMIVEVSRSACLMT
                     TQLICKLLRSREAAAAVDVRTWPLILDTDDLPKKRPAQICKPCNPDTLAYLDFSVSTTG
                     MLAGVKMSHAATSAFCRSIKLQCELYPSREVAICLDPYCGLGFVLWCLCSVYSGHQSIL
                     IPPSELETNPALWLLAVSQYKVRDTFCSYSVMELCTKGLGSQTESLKARGLDLSRVRTC
                     VVVAEERPRIALTQSFSKLFKDLGLHPRAVSTSFGCRVNLAICLQGTSGPDPTTVYVDM
                     RALRHDRVRLVERGSPHSLPLMESGKILPGVRIIIANPETKGPLGDSHLGEIWVHSAHN
                     ASGYFTIYGDESLQSDHFNSRLSFGDTQTIWARTGYLGFLRRTELTDANGERHDALYVV
                     GALDEAMELRGMRYHPIDIETSVIRAHKSVTECAVFTWTNLLVVVVELDGSEQEALDLV
                     PLVTNVVLEEHYLIVGVVVVVDIGVIPINSRGEKQRMHLRDGFLADQLDPIYVAYNM"
                     /note="DIP2 homolog C; DIP2 disco-interacting protein 2
                     homolog C"
                     /note="color: #993366"
                     /db_xref="{'GI': '44888818', 'CCDS': 'CCDS7054.1',
                     'GeneID': '22982', 'HGNC': 'HGNC:29150', 'MIM': '611380'}"
                     /gene_synonym="KIAA0934"
                     /transl_table=1
                     /protein_id="NP_055789.1"
                     /codon_start=1
                     /label="DIP2C"
     CDS             288..1318
                     /product="site-specific recombinase"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /transl_table=1
                     /protein_id=""
                     /codon_start=1
                     /label="Cre"
     CDS             5187..6047
                     /gene="bla"
                     /product="β-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /transl_table=1
                     /codon_start=1
                     /label="AmpR"
     rep_origin      6218..6806
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     rep_origin      4345..4800
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     terminator      3656..4087
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   4130..4270
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     promoter        5082..5186
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     CDS             3575..3640
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /transl_table=1
                     /codon_start=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     protein_bind    1367..1400
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /label="lox2272-JTZ17"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggcgattga
     1501 cagtatacat caagttggag tttattgcct ggccttggtg ccagcaaaca ccctccccaa
     1561 aaccccgctt ggtgggatcc atttatcaga aacaaaacag ctttttctgg agggctctct
     1621 gcacccctgc aatgtcctaa tgtgccccca cacctgcgtc acaaacttgc ctaagcctcg
     1681 acagaagcag ccagaaatcg gccctgcctc tgtgatggtg gggaacctgg tctctgggaa
     1741 gagaatcgcc caggccagtg gcagagacct gggtcagatc gaagataacg accaggcacg
     1801 caagttcctg ttcctctcag aggtcttgca gtggagagca cagaccaccc cggaccacat
     1861 cctctacacg ctgctcaact gtcggggtgc gatagcgaac tcgctgacct gcgtgcagct
     1921 gcacaagaga gctgagaaga tcgccgtgat gctgatggag aggggccacc ttcaggacgg
     1981 cgaccacgtg gccttggtct accccccagg aatagacctg atagcagcgt tttatggttg
     2041 cctgtacgca ggctgtgtgc caataaccgt ccgtcccccg cacccacaga acatcgcgac
     2101 gacgttgcct accgtcaaga tgattgtgga ggtgagtcgc tctgcctgtc tgatgacgac
     2161 acagctgatc tgtaagttgc tgcggtccag ggaggcggcg gcggctgtgg acgtcaggac
     2221 gtggcccctc atcctggaca cagatgattt gccaaagaag cggcctgccc agatctgcaa
     2281 accttgcaac ccagacactc ttgcatatct cgacttcagc gtgtccacaa ctgggatgct
     2341 agctggcgta aagatgtctc acgcagccac cagtgccttc tgccgttcca ttaagctgca
     2401 gtgtgaactt tacccctcta gagaagtggc catctgcctg gacccttact gtggactggg
     2461 atttgtcctc tggtgcctct gcagtgtgta ttctgggcac cagtccatcc tgatcccgcc
     2521 ctctgagctg gaaaccaacc ccgccttgtg gcttcttgcc gtgagtcagt acaaagtccg
     2581 agacacgttt tgctcctact ccgtgatgga gctgtgcacc aaggggctgg gctcgcaaac
     2641 agagtccctc aaggcgcgag ggctggactt gtcccgagtg aggacctgcg tggttgtggc
     2701 ggaagagagg cctcggatcg cactcacaca gtcgttctca aagctgttta aggacctggg
     2761 ccttcacccg cgggccgtca gcacctcgtt cggttgcagg gtgaacctgg cgatttgctt
     2821 gcagggaacc tcaggacctg acccaaccac tgtctacgtg gacatgagag ccctgagaca
     2881 cgacagagtc cgcttagtgg aaagaggatc ccctcatagt ctgcccctga tggaatcggg
     2941 aaagatactt ccaggggttc ggattataat tgccaaccca gaaacaaaag gaccgctggg
     3001 ggactcacac cttggagaga tttgggttca cagtgcccac aatgccagcg gttatttcac
     3061 tatttacgga gacgaatccc tccagtcaga tcacttcaac tcaagactaa gttttggaga
     3121 cacccagacc atctgggcac gcacaggcta cttggggttc ctgcggagaa ctgagctcac
     3181 agatgcaaat ggagagcgcc atgatgccct ctacgtggta ggggcactgg acgaagccat
     3241 ggagctgcgg ggcatgcggt accacccaat cgacattgag acctcggtca tcagagccca
     3301 taaaagcgtt acggaatgtg ctgtgtttac ctggacaaat ttgttggtgg ttgtggttga
     3361 gctggatggg tcggaacaag aagccttgga cctggttccc ttggtgacca acgtggtcct
     3421 ggaggagcac tacctgatcg tcggagtggt ggtcgtggtg gacatcggcg tcatccccat
     3481 caactcccgt ggggagaagc agcgcatgca cctgcgagac gggtttttgg cagaccagct
     3541 agaccccatc tatgtggcct acaacatgtg tacagactac aaagaccatg acggtgatta
     3601 taaagatcat gacatcgact acaaggatga cgatgacaag taagaattct gatgactcga
     3661 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3721 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3781 cgtatggctt tcattttctc ctccttgtat aaatcctggt tagttcttgc cacggcggaa
     3841 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3901 tccgtggtgt ttatttgtga aatttgtgat gctattgctt tatttgtaac catctagctt
     3961 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     4021 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt
     4081 tttttaaaga tctggagacc ggtgtggcac gtgcggaccg agcggccgca ggaaccccta
     4141 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
     4201 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
     4261 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     4321 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4381 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4441 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4501 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4561 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4621 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4681 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     4741 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     4801 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     4861 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     4921 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     4981 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     5041 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5101 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5161 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5221 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5281 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5341 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5401 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5461 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5521 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5581 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5641 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5701 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5761 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5821 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5881 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5941 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6001 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6061 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6121 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6181 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6241 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6301 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6361 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6421 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6481 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6541 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6601 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6661 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6721 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6781 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6841 ccttttgctg gccttttgct cacatgt
//
