LOCUS       .                       6610 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             760..3750
                     /protein_id="NP_001335697.1"
                     /note="isoform 1 is encoded by transcript variant 3;
                     NEDD4-related E3 ubiquitin ligase NEDL2; E3
                     ubiquitin-protein ligase HECW2; NEDD4-like E3
                     ubiquitin-protein ligase 2; HECT-type E3 ubiquitin
                     transferase HECW2"
                     /note="color: #993366"
                     /translation="ASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQRA
                     NSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANF
                     WDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVKNPAV
                     MMGAEGMEGGASGNLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPT
                     CAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLT
                     IPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEDASPEAVGTILGV
                     NSVNGDLGSPSDDEDMPGSHHDSQVCSNGPVSEDSAADGTPKHSFRTSSTLEIDTEELT
                     STSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTATCSERSMGASPKLRSSFPTDTRLNA
                     MLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEA
                     STHEAASFEDKPENLPELAESSLPAGPAPEEGEGGPEPQPSADQGSAELCGSQEVDQPT
                     SGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGES
                     DLECADSSCNESVTTQLSSVDTRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAE
                     GSQESVCTAGSLPVVQVPSGEDEGPGAESATVPDQEELGEVWQRRGSLEGAAAAAESPP
                     QEEGSAGEAQGTCEGATAQEEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNW
                     EARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTN
                     ERPEENTNAIDGAGEEADFHQASADFRRENILPHSTSRSRITLLLQSPPVKFLISPEFF
                     TVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRG
                     WEMKHDHQ"
                     /gene="HECW2"
                     /EC_number="2.3.2.26"
                     /db_xref="{'GI': '1149890059', 'CCDS': 'CCDS33354.1',
                     'GeneID': '57520', 'HGNC': 'HGNC:29853', 'MIM': '617245'}"
                     /product="E3 ubiquitin-protein ligase HECW2 isoform 1"
                     /codon_start=1
                     /gene_synonym="NDHSAL; NEDL2"
                     /transl_table=1
                     /label="HECW2"
     CDS             4930..5790
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /product="β-lactamase"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     rep_origin      5961..6549
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      4088..4543
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   3873..4013
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     promoter        4825..4929
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          3751..3791
                     /label="Splicing Donor"
                     /note="color: noColor"
     misc_feature    3795..3828
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     CDS             727..753
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /codon_start=1
                     /transl_table=1
                     /label="HA"
                     /note="color: #cc99b2"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatctg ctagttcagc ccgggagcac
      781 ctgctttttg tgaggcgtcg aaatccccag atgcggtaca cattgagccc agagaacctc
      841 cagagccttg ccgcccagag ctccatgcca gagaacatga ccctgcagcg ggccaacagc
      901 gacaccgacc tggtgacttc tgagagccgc tccagcttaa ctgccagcat gtacgagtac
      961 acgctggggc aagcccagaa cctcattatc ttctgggaca ttaaagagga ggtggacccc
     1021 agtgattgga ttggacttta tcatatagat gagaattctc cagccaactt ctgggattct
     1081 aagaacaggg gtgtgactgg aacacaaaaa gggcaaattg tatggagaat tgagcctggg
     1141 ccctatttca tggaaccgga gataaaaatc tgttttaaat attaccacgg cattagtgga
     1201 gccctgcgag ccacgacccc ctgcatcacc gtgaagaacc cagctgtgat gatgggggca
     1261 gaaggcatgg agggaggtgc ttcaggaaac ctgcattctc gaaaacttgt tagctttaca
     1321 ttgtcagatc ttagggcagt tgggctaaag aaagggatgt tcttcaatcc tgacccttat
     1381 cttaagatgt caattcagcc aggaaagaag agcagtttcc ccacctgtgc ccaccacggg
     1441 caggagagac ggtctactat catcagtaac accaccaatc caatttggca ccgagagaaa
     1501 tattcctttt ttgcacttct tactgatgtc ttagaaattg aaattaaaga caaatttgcc
     1561 aagagccgtc ccatcatcaa gcgttttctg gggaaactaa ccattccagt ccagaggctg
     1621 ctggagcgac aagccatcgg tgatcaaatg ctcagctaca accttggcag aaggctccca
     1681 gctgaccacg tgagtgggta cctccagttt aaagtggagg ttacgtcttc tgttcatgaa
     1741 gatgcctctc cagaagctgt tggcacaata cttggagtca attctgtgaa tggagactta
     1801 ggtagccctt ccgatgacga ggacatgcca gggagccatc acgacagcca ggtgtgctct
     1861 aatgggccag tttctgagga cagtgctgcc gatggaaccc ccaagcattc attcaggact
     1921 agctccactc tggaaataga cacagaggaa ttaacctcta cgtcttcaag gacctcacct
     1981 cccagaggac gtcaggattc actcaatgat tacttagatg ctatcgaaca caatggccac
     2041 tccaggccag ggacagcgac ctgctctgag aggtccatgg gtgcctctcc gaaactgagg
     2101 agtagttttc ccactgacac aagactcaat gccatgcttc atattgattc agatgaagaa
     2161 gatcacgagt tccagcaaga cctgggttac ccatcttcct tggaggagga gggaggcctg
     2221 atcatgttta gcagggcatc cagagctgat gatggaagcc tgacatctca gacaaagctg
     2281 gaggacaacc ctgttgagaa tgaggaagcc tccacacacg aagctgcttc ctttgaggat
     2341 aagccagaaa atcttccaga gcttgcagag agctccttac ctgcaggccc agccccagag
     2401 gaaggtgaag gcggcccaga gcctcaaccc agtgctgacc agggcagtgc tgaactgtgt
     2461 ggctctcaag aggtagatca gcccacaagt ggcgcagaca cagggacttc cgatgcatca
     2521 ggaggaagcc gaagagccgt cagtgagacc gagtctctcg atcagggctc tgagccttcc
     2581 caggtgtcct ctgaaacaga acccagtgat cctgccagga cagagagtgt gagcgaagcc
     2641 agcaccaggc ctgagggaga gagtgacctg gaatgcgctg acagctcctg caatgagagt
     2701 gtgaccacgc agctgtcctc tgtggacaca cggtgctcct ctcttgagag cgcacggttt
     2761 cctgaaaccc cagccttttc ttctcaggag gaggaagacg gagcctgtgc agcagagccc
     2821 accagcagtg gccctgccga agggtcgcag gaatccgtgt gcactgctgg ttctttacct
     2881 gtggtacagg tgcccagtgg ggaggatgaa gggccagggg cagaatcggc cactgtacct
     2941 gaccaggagg agctggggga ggtctggcag cggaggggga gcctggaggg agctgcagct
     3001 gctgccgaga gcccaccgca agaagaaggc agtgctgggg aggcccaagg cacctgtgaa
     3061 ggggcaactg cccaggagga gggcgctact ggaggttctc aagccaacgg ccaccagcca
     3121 ctgcgatcac taccttcagt gcgccaggat gttagccggt accagagggt ggacgaggct
     3181 ctcccaccaa actgggaggc acgcattgac agccacggca ggatcttcta cgtggatcac
     3241 gtaaacagaa ccacgacgtg gcagcgaccg acagctcccc cagccccgca ggtgctgcag
     3301 agatctaact ccatacagca aatggagcag ctgaaccggc gatatcagag tatccgcaga
     3361 accatgacca atgagaggcc tgaggaaaat accaatgcta ttgatggggc aggggaggaa
     3421 gctgactttc accaagccag tgcagatttc cgacgggaga acatcctgcc tcactctacc
     3481 tccagatcca ggatcacgtt gctgttacag tctccccccg tgaagttcct catcagccca
     3541 gagttcttca ccgtgctgca ttctaaccct agtgcctacc gcatgtttac aaacaacacg
     3601 tgtttgaagc acatgatcac caaagtccgg agggacaccc accactttga acgctaccag
     3661 cataaccgcg accttgtggg attcctcaac atgttcgcga acaaacagct agagctgccg
     3721 cggggatggg aaatgaaaca tgatcaccag gtaagtatca aggttacaag acaggtttaa
     3781 ggagaccaat aagctattat tcgtatagga tactttatac gaagttatta agttatggag
     3841 accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac
     3901 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     3961 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct
     4021 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
     4081 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4141 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4201 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4261 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     4321 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4381 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt
     4441 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4501 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
     4561 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     4621 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     4681 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     4741 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     4801 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4861 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4921 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4981 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5041 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5101 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5161 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5221 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5281 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5341 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5401 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5461 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5521 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5581 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     5641 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5701 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5761 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5821 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5881 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5941 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6001 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6061 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     6121 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6181 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6241 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6301 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6361 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6421 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6481 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6541 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6601 gctcacatgt
//
