LOCUS       .                       6088 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             760..3228
                     /protein_id="NP_001238953.1"
                     /note="isoform 3 is encoded by transcript variant 3;
                     transient receptor potential melastatin family;
                     melastatin-1; long transient receptor potential channel 1"
                     /note="color: #993366"
                     /translation="MGQKSWIEKTFCKRECIFVIPSMKDSNRCCCGQFTNQHIPPLPSA
                     TPSKNEEESKQVETQPEKWSVAKHTQSYPTDSYGVLEFQGGGYSNKAMYIRVSYDTKPD
                     SLLHLMVKDWQLELPKLLISVHGGLQNFEMQPKLKQVFGKGLIKAAMTTGAWIFTGGVS
                     TGVISHVGDALKDHSSKSRGRVCAIGIAPWGIVENKEDLVGKDVTRVYQTMSNPLSKLS
                     VLNNSHTHFILADNGTLGKYGAEVKLRRLLEKHISLQKINTRLGQGVPLVGLVVEGGPN
                     VVSIVLEYLQEEPPIPVVICDGSGRASDILSFAHKYCEEGGIINESLREQLLVTIQKTF
                     NYNKAQSHQLFAIIMECMKKKELVTVFRMGSEGQQDIEMAILTALLKGTNVSAPDQLSL
                     ALAWNRVDIARSQIFVFGPHWPPLGSLAPPTDSKATEKEKKPPMATTKGGRGKGKGKKK
                     GKVKEEVEEETDPRKIELLNWVNALEQAMLDALVLDRVDFVKLLIENGVNMQHFLTIPR
                     LEELYNTRLGPPNTLHLLVRDVKKSNLPPDYHISLIDIGLVLEYLMGGAYRCNYTRKNF
                     RTLYNNLFGPKRPKALKLLGMEDDEPPAKGKKKKKKKKEEEIDIDVDDPAVSRFQYPFH
                     ELMVWAVLMKRQKMAVFLWQRGEESMAKALVACKLYKAMAHESSESDLVDDISQDLDNN
                     SKDFGQLALELLDQSYKHDEQIAMKLLTYELKNWSNSTCLKLAVAAKHRDFIAHTCSQM
                     LLTDMWMGRLRMRKNPGLKVIMGILLPPTILFLEFRTYDDFSYQTSKENEDGKEKEEEN
                     TDANADAGSRK"
                     /gene="TRPM1"
                     /db_xref="{'GI': '354721154', 'CCDS': 'CCDS58346.1',
                     'GeneID': '4308', 'HGNC': 'HGNC:7146', 'MIM': '603576'}"
                     /product="transient receptor potential cation channel
                     subfamily M member 1 isoform 3"
                     /codon_start=1
                     /gene_synonym="CSNB1C; LTRPC1; MLSN1"
                     /transl_table=1
                     /label="TRPM1"
     CDS             4408..5268
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /product="β-lactamase"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     rep_origin      5439..6027
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      3566..4021
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   3351..3491
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     promoter        4303..4407
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          3229..3269
                     /label="Splicing Donor"
                     /note="color: noColor"
     misc_feature    3273..3306
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     CDS             727..753
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /codon_start=1
                     /transl_table=1
                     /label="HA"
                     /note="color: #cc99b2"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatcta tgggtcagaa atcttggata
      781 gagaaaacct tttgcaaacg ggaatgtatc tttgtaattc ctagcatgaa agactctaac
      841 aggtgttgct gtggccagtt caccaaccag catatccccc ctctgccaag tgcaacaccc
      901 agcaaaaatg aagaggaaag caaacaggtg gagactcagc ctgagaaatg gtctgttgcc
      961 aagcacaccc agagctaccc aacagattcc tatggagttc ttgaattcca gggtggcgga
     1021 tattccaata aagccatgta tatccgtgta tcctatgaca ccaagccaga ctcactgctc
     1081 catctcatgg tgaaagattg gcagctggaa ctccccaagc tcttaatatc tgtgcatgga
     1141 ggcctccaga actttgagat gcagcccaag ctgaaacaag tctttgggaa aggcctgatc
     1201 aaggctgcta tgaccaccgg ggcctggatc ttcaccgggg gtgtcagcac aggtgttatc
     1261 agccacgtag gggatgcctt gaaagaccac tcctccaagt ccagaggccg ggtttgtgct
     1321 ataggaattg ctccatgggg catcgtggag aataaggaag acctggttgg aaaggatgta
     1381 acaagagtgt accagaccat gtccaaccct ctaagtaagc tctctgtgct caacaactcc
     1441 cacacccact tcatcctggc tgacaatggc accctgggca agtatggcgc cgaggtgaag
     1501 ctgcgaaggc tgctggaaaa gcacatctcc ctgcagaaga tcaacacaag actggggcag
     1561 ggcgtgcccc tcgtgggtct cgtggtggag gggggcccta acgtggtgtc catcgtcttg
     1621 gaatacctgc aagaagagcc tcccatccct gtggtgattt gtgatggcag cggacgtgcc
     1681 tcggacatcc tgtcctttgc gcacaagtac tgtgaagaag gcggaataat aaatgagtcc
     1741 ctcagggagc agcttctagt taccattcag aaaacattta attataataa ggcacaatca
     1801 catcagctgt ttgcaattat aatggagtgc atgaagaaga aagaactcgt cactgtgttc
     1861 agaatgggtt ctgagggcca gcaggacatc gagatggcaa ttttaactgc cctgctgaaa
     1921 ggaacaaacg tatctgctcc agatcagctg agcttggcac tggcttggaa ccgcgtggac
     1981 atagcacgaa gccagatctt tgtctttggg ccccactggc cgcccctggg aagcctggca
     2041 cccccgacgg acagcaaagc cacggagaag gagaagaagc cacccatggc caccaccaag
     2101 ggaggaagag gaaaagggaa aggcaagaag aaagggaaag tgaaagagga agtggaggaa
     2161 gaaactgacc cccggaagat agagctgctg aactgggtga atgctttgga gcaagcgatg
     2221 ctagatgctt tagtcttaga tcgtgtcgac tttgtgaagc tcctgattga aaacggagtg
     2281 aacatgcaac actttctgac cattccgagg ctggaggagc tttataacac aagactgggt
     2341 ccaccaaaca cacttcatct gctggtgagg gatgtgaaaa agagcaacct tccgcctgat
     2401 taccacatca gcctcataga catcgggctc gtgctggagt acctcatggg aggagcctac
     2461 cgctgcaact acactcggaa aaactttcgg accctttaca acaacttgtt tggaccaaag
     2521 aggcctaaag ctcttaaact tctgggaatg gaagatgatg agcctccagc taaagggaag
     2581 aaaaagaaaa agaagaaaaa ggaggaagag atcgacattg atgtggacga ccctgccgtg
     2641 agtcggttcc agtatccctt ccacgagctg atggtgtggg cagtgctgat gaaacgccag
     2701 aaaatggcag tgttcctctg gcagcgaggg gaagagagca tggccaaggc cctggtggcc
     2761 tgcaagctct acaaggccat ggcccacgag tcctccgaga gtgatctggt ggatgacatc
     2821 tcccaggact tggataacaa ttccaaagac ttcggccagc ttgctttgga gttattagac
     2881 cagtcctata agcatgacga gcagatcgct atgaaactcc tgacctacga gctgaaaaac
     2941 tggagcaact cgacctgcct caaactggcc gtggcagcca aacaccggga cttcattgct
     3001 cacacctgca gccagatgct gctgaccgat atgtggatgg gaagactgcg gatgcggaag
     3061 aaccccggcc tgaaggttat catggggatt cttctacccc ccaccatctt gtttttggaa
     3121 tttcgcacat atgatgattt ctcgtatcaa acatccaagg aaaatgagga tggcaaagaa
     3181 aaagaagagg aaaatacgga tgcaaatgca gatgctggct caagaaaggt aagtatcaag
     3241 gttacaagac aggtttaagg agaccaataa gctattattc gtataggata ctttatacga
     3301 agttattaag ttatggagac cggtgtggca cgtgcggacc gagcggccgc aggaacccct
     3361 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
     3421 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
     3481 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     3541 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3601 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3661 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3721 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     3781 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     3841 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     3901 atctcgggct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     3961 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     4021 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     4081 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     4141 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     4201 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     4261 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4321 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4381 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4441 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4501 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4561 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     4621 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     4681 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     4741 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     4801 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     4861 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     4921 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     4981 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5041 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5101 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5161 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5221 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     5281 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5341 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5401 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5461 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5521 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5581 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5641 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5701 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5761 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5821 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5881 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5941 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6001 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6061 gccttttgct ggccttttgc tcacatgt
//
