LOCUS       .                       6493 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             760..3633
                     /translation="AKSGGCGAGAGVGGGNGALTWVTLFDQNNAAKKEESETANKNDSS
                     KKLSVERVYQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDEDVGMNCREVTFVPGLYKI
                     FDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFG
                     QLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTS
                     EAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKK
                     LPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATT
                     KGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKE
                     NMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGI
                     PKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREAS
                     HKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINF
                     IHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKG
                     LGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQ
                     RRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTC
                     FKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGT
                     RLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLIN
                     GAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVS
                     GEIFVVDRNTVEITELPVRTWTQVYKEQ"
                     /note="isoform 1 is encoded by transcript variant 1; DNA
                     topoisomerase 2-beta; topo II beta; DNA topoisomerase II,
                     180 kD; topoisomerase II beta; antigen MLAA-44; U937
                     associated antigen; topoisomerase IIb; DNA topoisomerase
                     II, beta isozyme; topoisomerase (DNA) II beta 180kDa"
                     /note="color: #993366"
                     /db_xref="{'GI': '1061214220', 'CCDS': 'CCDS82746.1',
                     'GeneID': '7155', 'HGNC': 'HGNC:11990', 'MIM': '126431'}"
                     /EC_number="5.6.2.2"
                     /gene="TOP2B"
                     /product="DNA topoisomerase 2-beta isoform 1"
                     /gene_synonym="BILU; top2beta; TOPIIB"
                     /transl_table=1
                     /codon_start=1
                     /protein_id="NP_001317629.1"
                     /label="TOP2B"
     CDS             4813..5673
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /gene="bla"
                     /product="β-lactamase"
                     /transl_table=1
                     /codon_start=1
                     /label="AmpR"
     rep_origin      5844..6432
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      3971..4426
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   3756..3896
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     promoter        4708..4812
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          3634..3674
                     /label="Splicing Donor"
                     /note="color: noColor"
     misc_feature    3678..3711
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     CDS             727..753
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /codon_start=1
                     /label="HA"
                     /note="color: #cc99b2"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatctg ccaagtcggg tggctgcggc
      781 gcgggagccg gcgtgggcgg cggcaacggg gcactgacct gggtgactct ttttgatcag
      841 aacaatgctg caaaaaaaga agagtcagaa actgccaaca aaaatgattc ttcaaagaag
      901 ttgtctgttg agagagtgta tcagaagaag acacaacttg aacacattct tcttcgtcct
      961 gatacatata ttgggtcagt ggagccattg acgcagttca tgtgggtgta tgatgaagat
     1021 gtaggaatga attgcaggga ggttaccttt gtgccaggtt tatacaagat ctttgatgaa
     1081 attttggtta atgctgctga caataaacag agggataaga acatgacttg tattaaagtt
     1141 tctattgatc ctgaatctaa cattataagc atttggaata atgggaaagg cattccagta
     1201 gtagaacaca aggtagagaa agtttatgtt cctgctttaa tttttggaca gcttttaaca
     1261 tccagtaact atgatgatga tgagaaaaaa gttacaggtg gtcgtaatgg ttatggtgca
     1321 aaactttgta atattttcag tacaaagttt acagtagaaa cagcttgcaa agaatacaaa
     1381 cacagtttta agcagacatg gatgaataat atgatgaaga cttctgaagc caaaattaaa
     1441 cattttgatg gtgaagatta cacatgcata acattccaac cagatctgtc caaatttaag
     1501 atggaaaaac ttgacaagga tattgtggcc ctcatgacta gaagggcata tgatttggct
     1561 ggttcgtgta gaggggtcaa ggtcatgttt aatggaaaga aattgcctgt aaatggattt
     1621 cgcagttatg tagatcttta tgtgaaagac aaattggatg aaactggggt ggccctgaaa
     1681 gttattcatg agcttgcaaa tgaaagatgg gatgtttgtc tcacattgag tgaaaaagga
     1741 ttccagcaaa tcagctttgt aaatagtatt gcaactacaa aaggtggacg gcacgtggat
     1801 tatgtggtag atcaagttgt tggtaaactg attgaagtag ttaagaaaaa gaacaaagct
     1861 ggtgtatcag tgaaaccatt tcaagtaaaa aaccatatat gggtttttat taattgcctt
     1921 attgaaaatc caacttttga ttctcagact aaggaaaaca tgactctgca gcccaaaagt
     1981 tttgggtcta aatgccagct gtcagaaaaa ttttttaaag cagcctctaa ttgtggcatt
     2041 gtagaaagta tcctgaactg ggtgaaattt aaggctcaga ctcagctgaa taagaagtgt
     2101 tcatcagtaa aatacagtaa aatcaaaggt attcccaaac tggatgatgc taatgatgct
     2161 ggtggtaaac attccctgga gtgtacactg atattaacag agggagactc tgccaaatca
     2221 ctggctgtgt ctggattagg tgtgattgga cgagacagat acggagtttt tccactcagg
     2281 ggcaaaattc ttaatgtacg ggaagcttct cataaacaga tcatggaaaa tgctgaaata
     2341 aataatatta ttaaaatagt tggtctacaa tataagaaaa gttacgatga tgcagaatct
     2401 ctgaaaacct tacgctatgg aaagattatg attatgaccg atcaggatca agatggttct
     2461 cacataaaag gcctgcttat taatttcatc catcacaatt ggccatcact tttgaagcat
     2521 ggttttcttg aagagttcat tactcctatt gtaaaggcaa gcaaaaataa gcaggaactt
     2581 tccttctaca gtattcctga atttgacgaa tggaaaaaac atatagaaaa ccagaaagcc
     2641 tggaaaataa agtactataa aggattgggt actagtacag ctaaagaagc aaaggaatat
     2701 tttgctgata tggaaaggca tcgcatcttg tttagatatg ctggtcctga agatgatgct
     2761 gccattacct tggcatttag taagaagaag attgatgaca gaaaagaatg gttaacaaat
     2821 tttatggaag accggagaca gcgtaggcta catggcttac cagagcaatt tttatatggt
     2881 actgcaacaa agcatttgac ttataatgat ttcatcaaca aggaattgat tctcttctca
     2941 aactcagaca atgaaagatc tataccatct cttgttgatg gctttaaacc tggccagcgg
     3001 aaagttttat ttacctgttt caagaggaat gataaacgtg aagtaaaagt tgcccagttg
     3061 gctggctctg ttgctgagat gtcggcttat catcatggag aacaagcatt gatgatgact
     3121 attgtgaatt tggctcagaa ctttgtggga agtaacaaca ttaacttgct tcagcctatt
     3181 ggtcagtttg gaactcggct tcatggtggc aaagatgctg caagccctcg ttatattttc
     3241 acaatgttaa gcactttagc aaggctactt tttcctgctg tggatgacaa cctccttaag
     3301 ttcctttatg atgataatca acgtgtagag cctgagtggt atattcctat aattcccatg
     3361 gttttaataa atggtgctga gggcattggt actggatggg cttgtaaact acccaactat
     3421 gatgctaggg aaattgtgaa caatgtcaga cgaatgctag atggcctgga tcctcatccc
     3481 atgcttccaa actacaaaaa ctttaaaggc acgattcaag aacttggtca aaaccagtat
     3541 gcagtcagtg gtgaaatatt tgtagtggac agaaacacag tagaaattac agagcttcca
     3601 gttagaactt ggacacaggt atataaagaa caggtaagta tcaaggttac aagacaggtt
     3661 taaggagacc aataagctat tattcgtata ggatacttta tacgaagtta ttaagttatg
     3721 gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc
     3781 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
     3841 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
     3901 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
     3961 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4021 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4081 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4141 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     4201 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4261 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct
     4321 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4381 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
     4441 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     4501 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     4561 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     4621 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     4681 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4741 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4801 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4861 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4921 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4981 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5041 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5101 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5161 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5221 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5281 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5341 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5401 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5461 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5521 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5581 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5641 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5701 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5761 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5821 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5881 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5941 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     6001 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6061 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6121 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6181 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6241 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6301 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6361 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6421 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6481 tttgctcaca tgt
//
