LOCUS       .                       6780 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1493..3493
                     /protein_id="NP_001317629.1"
                     /note="isoform 1 is encoded by transcript variant 1; DNA
                     topoisomerase 2-beta; topo II beta; DNA topoisomerase II,
                     180 kD; topoisomerase II beta; antigen MLAA-44; U937
                     associated antigen; topoisomerase IIb; DNA topoisomerase
                     II, beta isozyme; topoisomerase (DNA) II beta 180kDa"
                     /note="color: #993366"
                     /translation="VLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAA
                     GLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLG
                     AESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEED
                     ETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSP
                     SDLWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGR
                     RIIPEITAMKADASKKLLKKKKGDLDTAAVKVEFDEEFSGAPVEGAGEEALTPSVPINK
                     GPKPKREKKEPGTRVRKTPTSSGKPSAKKVKKRNPWSDDESKSESDLEETEPVVIPRDS
                     LLRRAAAERPKYTFDFSEEEDDDADDDDDDNNDLEELKVKASPITNDGEDEFVPSDGLD
                     KDEYTFSPGKSKATPEKSLHDKKSQDFGNLFSFPSYSQKSEDDSAKFDSNEEDSASVFS
                     PSFGLKQTDKVPSKTVAAKKGKPSSDTVPKPKRAPKQKKVVEAVNSDSDSEFGIPKKTT
                     TPKGKGRGAKKRKASGSENEGDYNPGRKTSKTTSKKPKKTSFDQDSDVDIFPSDFPTEP
                     PSLPRTGRARKEVKYFAESDEEEDDVDFAMFN"
                     /gene="TOP2B"
                     /EC_number="5.6.2.2"
                     /db_xref="{'GI': '1061214220', 'CCDS': 'CCDS82746.1',
                     'GeneID': '7155', 'HGNC': 'HGNC:11990', 'MIM': '126431'}"
                     /product="DNA topoisomerase 2-beta isoform 1"
                     /codon_start=1
                     /gene_synonym="BILU; top2beta; TOPIIB"
                     /transl_table=1
                     /label="TOP2B"
     CDS             288..1318
                     /protein_id=""
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /product="site-specific recombinase"
                     /codon_start=1
                     /transl_table=1
                     /label="Cre"
     CDS             5100..5960
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /product="β-lactamase"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     rep_origin      6131..6719
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     rep_origin      4258..4713
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     terminator      3569..4000
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   4043..4183
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     promoter        4995..5099
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     CDS             3500..3565
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /codon_start=1
                     /transl_table=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     protein_bind    1367..1400
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /bound_moiety="Cre recombinase"
                     /label="lox2272-JTZ17"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggttttaga
     1501 acctatgcta aatggaacag ataaaacacc agcattaatt tctgattata aagaatatca
     1561 tactgacaca actgtgaaat ttgtggtgaa aatgactgaa gagaaactag cacaagcaga
     1621 agctgctgga ctgcataaag tttttaaact tcaaactact cttacttgta attccatggt
     1681 actttttgat catatgggat gtctgaagaa atatgaaact gtgcaagaca ttctgaaaga
     1741 attctttgat ttacgattaa gttattacgg tttacgtaag gagtggcttg tgggaatgtt
     1801 gggagcagaa tctacaaagc ttaacaatca agcccgtttc attttagaga agatacaagg
     1861 gaaaattact atagagaata ggtcaaagaa agatttgatt caaatgttag tccagagagg
     1921 ttatgaatct gacccagtga aagcctggaa agaagcacaa gaaaaggcag cagaagagga
     1981 tgaaacacaa aaccagcatg atgatagttc ctccgattca ggaactcctt caggcccaga
     2041 ttttaattat attttaaata tgtctctgtg gtctcttact aaagaaaaag ttgaagaact
     2101 gattaaacag agagatgcaa aagggcgaga ggtcaatgat cttaaaagaa aatctccttc
     2161 agatctttgg aaagaggatt tagcggcatt tgttgaagaa ctggataaag tggaatctca
     2221 agaacgagaa gatgttctgg ctggaatgtc tggaaaagca attaaaggta aagttggcaa
     2281 acctaaggtg aagaaactcc agttggaaga gacaatgccc tcaccttatg gcagaagaat
     2341 aattcctgaa attacagcta tgaaggcaga tgccagcaaa aagttgctga agaagaagaa
     2401 gggtgatctt gatactgcag cagtaaaagt ggaatttgat gaagaattca gtggagcacc
     2461 agtagaaggt gcaggagaag aggcattgac tccatcagtt cctataaata aaggtcccaa
     2521 acctaagagg gagaagaagg agcctggtac cagagtgaga aaaacaccta catcatctgg
     2581 taaacctagt gcaaagaaag tgaagaaacg gaatccttgg tcagatgatg aatccaagtc
     2641 agaaagtgat ttggaagaaa cagaacctgt ggttattcca agagattctt tgcttaggag
     2701 agcagcagcc gaaagaccta aatacacatt tgatttctca gaagaagagg atgatgatgc
     2761 tgatgatgat gatgatgaca ataatgattt agaggaattg aaagttaaag catctcccat
     2821 aacaaatgat ggggaagatg aatttgttcc ttcagatggg ttagataaag atgaatatac
     2881 attttcacca ggcaaatcaa aagccactcc agaaaaatct ttgcatgaca aaaaaagtca
     2941 ggattttgga aatctcttct catttccttc atattctcag aagtcagaag atgattcagc
     3001 taaatttgac agtaatgaag aagattctgc ttctgttttt tcaccatcat ttggtctgaa
     3061 acagacagat aaagttccaa gtaaaacggt agctgctaaa aagggaaaac cgtcttcaga
     3121 tacagtccct aagcccaaga gagccccaaa acagaagaaa gtagtagagg ctgtaaactc
     3181 tgactcggat tcagaatttg gcattccaaa gaagactaca acaccaaaag gtaaaggccg
     3241 aggggcaaag aaaaggaaag catctggctc tgaaaatgaa ggcgattata accctggcag
     3301 gaaaacatcc aaaacaacaa gcaagaaacc gaagaagaca tcttttgatc aggattcaga
     3361 tgtggacatc ttcccctcag acttccctac tgagccacct tctctgccac gaaccggtcg
     3421 ggctaggaaa gaagtaaaat attttgcaga gtctgatgaa gaagaagatg atgttgattt
     3481 tgcaatgttt aattgtacag actacaaaga ccatgacggt gattataaag atcatgacat
     3541 cgactacaag gatgacgatg acaagtaact cgagataatc aacctctgga ttacaaaatt
     3601 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3661 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3721 tataaatcct ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3781 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgtttatttg tgaaatttgt
     3841 gatgctattg ctttatttgt aaccatctag ctttatttgt gaaatttgtg atgctattgc
     3901 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     3961 tatgtttcag gttcaggggg agatgtggga ggttttttaa agatctggag accggtgtgg
     4021 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
     4081 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
     4141 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat
     4201 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
     4261 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4321 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4381 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     4441 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
     4501 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4561 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag
     4621 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4681 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
     4741 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     4801 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     4861 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     4921 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     4981 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     5041 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     5101 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     5161 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     5221 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     5281 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     5341 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     5401 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     5461 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     5521 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     5581 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     5641 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5701 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     5761 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     5821 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5881 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     5941 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     6001 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     6061 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6121 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6181 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6241 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     6301 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6361 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6421 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6481 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6541 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6601 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6661 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6721 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
