LOCUS       .                       6868 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    4053..4086
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     intron          4009..4049
                     /label="Splicing Donor"
                     /note="color: noColor"
     promoter        5083..5187
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   4131..4271
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     rep_origin      4346..4801
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      6219..6807
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     CDS             5188..6048
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /product="β-lactamase"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     CDS             760..4008
                     /protein_id="NP_009029.3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     Clathrin, heavy polypeptide D; clathrin, heavy
                     polypeptide-like 1; clathrin heavy chain 2; CLH-22;
                     clathrin heavy chain on chromosome 22"
                     /note="color: #993366"
                     /translation="MAQILPVRFQEHFQLQNLGINPANIGFSTLTMESDKFICIREKVG
                     EQAQVTIIDMSDPMAPIRRPISAESAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTM
                     AEEVIFWKWVSVNTVALVTETAVYHWSMEGDSQPMKMFDRHTSLVGCQVIHYRTDEYQK
                     WLLLVGISAQQNRVVGAMQLYSVDRKVSQPIEGHAAAFAEFKMEGNAKPATLFCFAVRN
                     PTGGKLHIIEVGQPAAGNQPFVKKAVDVFFPPEAQNDFPVAMQIGAKHGVIYLITKYGY
                     LHLYDLESGVCICMNRISADTIFVTAPHKPTSGIIGVNKKGQVLSVCVEEDNIVNYATN
                     VLQNPDLGLRLAVRSNLAGAEKLFVRKFNTLFAQGSYAEAAKVAASAPKGILRTRETVQ
                     KFQSIPAQSGQASPLLQYFGILLDQGQLNKLESLELCHLVLQQGRKQLLEKWLKEDKLE
                     CSEELGDLVKTTDPMLALSVYLRANVPSKVIQCFAETGQFQKIVLYAKKVGYTPDWIFL
                     LRGVMKISPEQGLQFSRMLVQDEEPLANISQIVDIFMENSLIQQCTSFLLDALKNNRPA
                     EGLLQTWLLEMNLVHAPQVADAILGNKMFTHYDRAHIAQLCEKAGLLQQALEHYTDLYD
                     IKRAVVHTHLLNPEWLVNFFGSLSVEDSVECLHAMLSANIRQNLQLCVQVASKYHEQLG
                     TQALVELFESFKSYKGLFYFLGSIVNFSQDPDVHLKYIQAACKTGQIKEVERICRESSC
                     YNPERVKNFLKEAKLTDQLPLIIVCDRFGFVHDLVLYLYRNNLQRYIEIYVQKVNPSRT
                     PAVIGGLLDVDCSEEVIKHLIMAVRGQFSTDELVAEVEKRNRLKLLLPWLESQIQEGCE
                     EPATHNALAKIYIDSNNSPECFLRENAYYDSSVVGRYCEKRDPHLACVAYERGQCDLEL
                     IKVCNENSLFKSEARYLVCRKDPELWAHVLEETNPSRRQLIDQVVQTALSETRDPEEIS
                     VTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYISRLD
                     NYDALDIASIAVSSALYEEAFTVFHKFDMNASAIQ"
                     /gene="CLTCL1"
                     /db_xref="{'GI': '242246985', 'CCDS': 'CCDS46662.2',
                     'GeneID': '8218', 'HGNC': 'HGNC:2093', 'MIM': '601273'}"
                     /product="clathrin heavy chain 2 isoform 1"
                     /codon_start=1
                     /gene_synonym="CHC22; CLH22; CLTCL; CLTD"
                     /transl_table=1
                     /label="CLTCL1"
     CDS             727..753
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /codon_start=1
                     /transl_table=1
                     /label="HA"
                     /note="color: #cc99b2"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatcta tggcgcagat cctccctgtt
      781 cgctttcagg agcacttcca gctccaaaac cttggaatta atccagctaa cattggattc
      841 agcacactga ccatggaatc tgacaagttc atatgtatcc gagagaaagt tggtgagcag
      901 gcacaggtca cgatcattga catgagtgac ccaatggctc cgatccgacg gcctatctct
      961 gcagagagtg ccatcatgaa tccagcctct aaggtgatag ctctgaaagc tgggaagaca
     1021 cttcagatct ttaatattga gatgaagagt aaaatgaagg ctcatactat ggcagaagaa
     1081 gtgattttct ggaaatgggt ttctgtgaac actgttgcct tggtgaccga gaccgcggtc
     1141 taccactgga gcatggaagg tgactcccag cccatgaaga tgtttgatag acataccagt
     1201 ctggtgggct gccaggtgat tcactaccgg actgatgagt accagaagtg gctgctgctc
     1261 gtaggcatct cggctcagca aaaccgtgtg gttggagcaa tgcagctcta ctctgtggat
     1321 aggaaggttt cacaacccat agaaggccat gctgcggctt ttgcagagtt caagatggag
     1381 gggaatgcca agcctgccac ccttttctgc tttgctgtac gtaatcccac aggaggcaag
     1441 ttgcacatca ttgaagttgg acagcctgca gcgggaaacc aaccttttgt aaagaaagca
     1501 gtagatgtgt tttttcctcc agaggcacag aatgattttc cagtggctat gcagattgga
     1561 gctaaacatg gtgttattta cttgatcaca aagtatggct atcttcatct gtacgaccta
     1621 gagtctggcg tgtgcatctg catgaaccgt attagtgctg acacaatatt tgtcactgct
     1681 ccacacaaac caacctctgg aattattggt gtcaacaaaa agggacaggt actgtcagtt
     1741 tgtgttgagg aagataacat tgtgaattat gcaaccaacg tgcttcagaa tccagacctt
     1801 ggtctgcgtt tggccgttcg tagtaacctg gctggggcag agaagttgtt tgtgagaaaa
     1861 ttcaataccc tctttgcaca gggcagctat gctgaagccg ccaaagttgc agcgtctgca
     1921 ccaaagggaa tcctgcgtac cagagagacg gtccagaaat tccagagtat acccgctcag
     1981 tctggccagg cttctccatt gctgcagtac ttcggaatcc tgctcgacca gggtcagctc
     2041 aataaacttg aatccttaga actttgccat ctggttcttc agcaggggcg taagcaactc
     2101 ctagagaagt ggctgaaaga agataagctg gagtgctcag aggagctcgg agacttggtc
     2161 aaaaccactg accccatgct cgctctgagt gtgtaccttc gggcaaatgt gccaagcaaa
     2221 gtgatccagt gttttgcaga aacaggccaa ttccagaaaa ttgtgctcta tgccaaaaag
     2281 gttgggtaca ccccagactg gatctttctg ctgaggggtg taatgaagat cagtccggaa
     2341 cagggcctgc agttttctcg aatgctagtg caggacgagg agccgctggc caacattagc
     2401 cagattgtgg acattttcat ggaaaacagt ttaattcagc agtgtacttc cttcttattg
     2461 gatgccttga agaataatcg cccagctgag ggactcctgc agacatggct gttggagatg
     2521 aaccttgttc atgcacccca ggttgcagat gccatccttg gaaataaaat gtttactcat
     2581 tacgaccggg cccacattgc ccagctctgt gagaaggcag gcctcctgca gcaagcactg
     2641 gagcactaca ccgacctcta tgacatcaag agggctgtgg tccacactca cctcctcaat
     2701 cccgagtggc ttgtcaattt ctttggctcc ttatcggtgg aggattctgt ggagtgtctg
     2761 catgccatgc tgtctgctaa catcagacag aaccttcagc tgtgtgtgca ggtggcctct
     2821 aagtaccacg agcagctggg cacgcaggcc ctggtggagc tctttgaatc cttcaagagt
     2881 tacaaaggcc tcttctactt cctgggctca atcgtgaact tcagccaaga cccagatgtg
     2941 catctgaaat acattcaggc tgcctgtaag acagggcaga tcaaggaggt ggagaggata
     3001 tgccgagaga gcagctgcta caacccagag cgtgtgaaga acttcctgaa ggaggccaag
     3061 ctcacagacc agcttcccct catcatcgtg tgtgatcgtt ttggctttgt ccatgacctt
     3121 gtcctatatt tataccgcaa caacctgcag aggtacattg agatctacgt gcagaaggtc
     3181 aaccctagcc ggaccccagc tgtgattgga gggctgcttg atgtggattg ttctgaggaa
     3241 gtgattaaac acttaatcat ggcagtgaga ggacagttct ctactgatga gttggtggct
     3301 gaagtagaaa aaagaaatag gctcaagctg ctgcttccct ggctggagtc ccagattcag
     3361 gaaggctgtg aggagcctgc cactcacaat gcactggcta aaatctacat cgacagcaac
     3421 aacagccccg agtgcttcct gagagagaat gcctactatg acagcagcgt ggtgggccgc
     3481 tactgtgaga agcgagaccc ccatctggcc tgtgttgcct atgagcgggg gcagtgtgac
     3541 cttgagctca tcaaggtgtg caatgagaat tctctgttca aaagcgaggc ccgctacctg
     3601 gtatgcagaa aggatccgga gctctgggct cacgtccttg aggagaccaa cccatccagg
     3661 agacagctaa ttgaccaggt ggtacagaca gcattgtcag aaacacggga tcctgaagag
     3721 atttcggtca ctgtcaaagc ctttatgaca gccgacctgc ctaatgaact gattgaactg
     3781 ctggagaaga tagttctgga taactctgtc ttcagcgagc acaggaatct acagaatctg
     3841 ttgatcctga ctgccatcaa ggcagaccgc acacgggtca tggagtacat cagccgcctg
     3901 gacaactatg acgcactgga catcgcgagc atcgctgtca gcagcgcact gtatgaggag
     3961 gccttcaccg ttttccacaa gtttgatatg aatgcctcag caatccaggt aagtatcaag
     4021 gttacaagac aggtttaagg agaccaataa gctattattc gtataggata ctttatacga
     4081 agttattaag ttatggagac cggtgtggca cgtgcggacc gagcggccgc aggaacccct
     4141 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
     4201 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
     4261 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     4321 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4381 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4441 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4501 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4561 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4621 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4681 atctcgggct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4741 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     4801 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     4861 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     4921 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     4981 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     5041 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5101 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5161 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5221 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5281 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5341 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5401 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     5461 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5521 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5581 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5641 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5701 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     5761 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5821 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5881 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5941 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6001 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6061 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6121 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6181 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6241 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6301 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6361 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6421 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6481 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6541 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6601 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6661 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6721 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6781 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6841 gccttttgct ggccttttgc tcacatgt
//
