LOCUS       .                       6450 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    1367..1400
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /bound_moiety="Cre recombinase"
                     /label="lox2272-JTZ17"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     CDS             288..1318
                     /protein_id=""
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /product="site-specific recombinase"
                     /codon_start=1
                     /transl_table=1
                     /label="Cre"
     CDS             3170..3235
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /codon_start=1
                     /transl_table=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     promoter        4665..4769
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   3713..3853
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     terminator      3239..3670
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     rep_origin      3928..4383
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     rep_origin      5801..6389
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     CDS             4770..5630
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /product="β-lactamase"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     CDS             1493..3163
                     /protein_id="NP_009029.3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     Clathrin, heavy polypeptide D; clathrin, heavy
                     polypeptide-like 1; clathrin heavy chain 2; CLH-22;
                     clathrin heavy chain on chromosome 22"
                     /note="color: #993366"
                     /translation="VLIEHIGNLDRAYEFAERCNEPAVWSQLAQAQLQKDLVKEAINSY
                     IRGDDPSSYLEVVQSASRSNNWEDLVKFLQMARKKGRESYIETELIFALAKTSRVSELE
                     DFINGPNNAHIQQVGDRCYEEGMYEAAKLLYSNVSNFARLASTLVHLGEYQAAVDNSRK
                     ASSTRTWKEVCFACMDGQEFRFAQLCGLHIVIHADELEELMCYYQDRGYFEELILLLEA
                     ALGLERAHMGMFTELAILYSKFKPQKMLEHLELFWSRVNIPKVLRAAEQAHLWAELVFL
                     YDKYEEYDNAVLTMMSHPTEAWKEGQFKDIITKVANVELCYRALQFYLDYKPLLINDLL
                     LVLSPRLDHTWTVSFFSKAGQLPLVKPYLRSVQSHNNKSVNEALNHLLTEEEDYQGLRA
                     SIDAYDNFDNISLAQQLEKHQLMEFRCIAAYLYKGNNWWAQSVELCKKDHLYKDAMQHA
                     AESRDAELAQKLLQWFLEEGKRECFAACLFTCYDLLRPDMVLELAWRHNLVDLAMPYFI
                     QVMREYLSKVDKLDALESLRKQEEHVTEPAPLVFDFDGHE"
                     /gene="CLTCL1"
                     /db_xref="{'GI': '242246985', 'CCDS': 'CCDS46662.2',
                     'GeneID': '8218', 'HGNC': 'HGNC:2093', 'MIM': '601273'}"
                     /product="clathrin heavy chain 2 isoform 1"
                     /codon_start=1
                     /gene_synonym="CHC22; CLH22; CLTCL; CLTD"
                     /transl_table=1
                     /label="CLTCL1"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtcctgat
     1501 cgagcacatt ggaaacctgg accgggcata tgagtttgcg gagagatgca atgagcctgc
     1561 tgtgtggagt cagctggccc aagcccagct ccagaaagat ttggtgaagg aagccatcaa
     1621 ctcctatatc agaggggacg acccttcctc ttacctggaa gttgttcagt cagccagcag
     1681 gagcaacaac tgggaggatc tagttaaatt tctgcagatg gccaggaaaa agggccgtga
     1741 gtcctatata gagactgaac ttatttttgc cttggctaaa accagccgtg tttctgagct
     1801 agaagatttt attaatggac ccaacaatgc ccacatccag caggttggag accgctgtta
     1861 cgaggaggga atgtacgagg ctgccaagct gctctatagc aatgtttcta actttgcccg
     1921 cctggcttcc accttggttc acctcggtga gtatcaggca gcagtggaca acagccgcaa
     1981 ggccagcagc acccggacgt ggaaggaggt gtgctttgcc tgcatggatg gacaagagtt
     2041 ccgcttcgca cagctgtgtg gtcttcacat cgtcattcat gcagatgagc tggaggagct
     2101 gatgtgctat taccaggatc gtggctactt tgaggagctg atcttgctgt tggaagcggc
     2161 cctgggcctg gagcgggccc acatgggcat gttcactgag ctggccatcc tctactccaa
     2221 attcaagcca cagaagatgc tggagcatct ggagcttttc tggtcccgtg tcaacatccc
     2281 aaaggtgctg agggctgcag agcaggcaca cctgtgggct gagctggtgt tcctctatga
     2341 caagtacgag gagtatgaca atgctgtgct caccatgatg agccacccca ctgaggcctg
     2401 gaaggagggt cagttcaagg acatcattac caaggttgcc aacgtcgagc tctgttacag
     2461 agccctgcag ttctatttgg attacaaacc actgctcatc aatgacctgc tgctggtgct
     2521 ttcaccccgg ctggaccaca cctggacagt cagtttcttt tcaaaggcag gtcagctgcc
     2581 cctggtgaag ccttacctgc ggtcagtcca gagccacaac aacaagagtg tgaatgaggc
     2641 actcaaccac ctgctgacag aggaggagga ctatcagggc ttaagggcat ctatcgatgc
     2701 ctatgacaac tttgacaaca tcagcctggc tcagcagctg gagaagcatc agctgatgga
     2761 gttcaggtgc attgcggcct atctgtacaa gggcaataac tggtgggccc agagcgtgga
     2821 gctctgcaag aaggatcatc tctacaagga tgccatgcag catgctgcag agtcgcggga
     2881 tgctgagctg gcccagaagt tgctgcagtg gttcctggag gaaggcaaga gggagtgctt
     2941 cgcagcttgt ctcttcacct gctatgacct gcttcgccca gacatggtgc ttgagctggc
     3001 ctggaggcac aacctcgtgg acttggccat gccctacttc atccaggtga tgagggagta
     3061 cctgagcaag gtggacaaac tggatgcctt ggagagtctg cgcaagcaag aggagcatgt
     3121 gacagagcct gcccctctcg tgtttgattt tgatgggcat gaatgtacag actacaaaga
     3181 ccatgacggt gattataaag atcatgacat cgactacaag gatgacgatg acaagtaact
     3241 cgagataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     3301 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     3361 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttagttct tgccacggcg
     3421 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3481 aattccgtgg tgtttatttg tgaaatttgt gatgctattg ctttatttgt aaccatctag
     3541 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     3601 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga
     3661 ggttttttaa agatctggag accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc
     3721 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     3781 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     3841 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     3901 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     3961 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4021 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4081 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4141 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4201 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     4261 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     4321 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     4381 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     4441 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     4501 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     4561 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     4621 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4681 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4741 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4801 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4861 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4921 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4981 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5041 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5101 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5161 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5221 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5281 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5341 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5401 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5461 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5521 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5581 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5641 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5701 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5761 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5821 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5881 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5941 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6001 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6061 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6121 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6181 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6241 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6301 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6361 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6421 tggccttttg ctggcctttt gctcacatgt
//
