LOCUS       .                       6676 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             760..3816
                     /translation="SLHSTHNRNNSGDILDIPSSQNSSSLNALTHSSRLKLHLKSDMSE
                     CENDDPLLRSAGKVRDINRTYVISASRKTADMPLTPNPVGRLALQRRTTRNKESSLLVS
                     ELEDTTEKTAETRLTLQRRAKTDSAEKWKTAEIDSVKMTLNVGGETENNGVSKESRTNV
                     RIVNNAKNSFVASSVPLDEDPQVIEMMADKKYKETFSAPSRANENVALKYSSNRPPIAS
                     LSQTEVVRSGHLTTKPTQSKLDIKVLGTGNLYHRSIGKEIAKTSNKFGSLEKRTPTKCT
                     TEHKLTTKCSLPQLKSPAPSILKNRMSNLQVKQRPKSSFLANKQERSAENTILPEEETV
                     VQNTSAGKDPLKVENSQVTVAVRVRPFTKREKIEKASQVVFMSGKEITVEHPDTKQVYN
                     FIYDVSFWSFDECHPHYASQTTVYEKLAAPLLERAFEGFNTCLFAYGQTGSGKSYTMMG
                     FSEEPGIIPRFCEDLFSQVARKQTQEVSYHIEMSFFEVYNEKIHDLLVCKDENGQRKQP
                     LRVREHPVYGPYVEALSMNIVSSYADIQSWLELGNKQRATAATGMNDKSSRSHSVFTLV
                     MTQTKTEFVEGEEHDHRITSRINLIDLAGSERCSTAHTNGDRLKEGVSINKSLLTLGKV
                     ISALSEQANQRSVFIPYRESVLTWLLKESLGGNSKTAMIATISPAASNIEETLSTLRYA
                     NQARLIVNIAKVNEDMNAKLIRELKAEIAKLKAAQRNSRNIDPERYRLCRQEITSLRMK
                     LHQQERDMAEMQRVWKEKFEQAEKRKLQETKELQKAGIMFQMDNHLPNLVNLNEDPQLS
                     EMLLYMIKEGTTTVGKYKPNSSHDIQLSGVLIADDHCTIKNFGGTVSIIPVGEAKTYVN
                     GKHILEITVLRHGDRVILGGDHYFRFNHPVEVQKGKRPSGRDTPISEGPKDFEFAKNEL
                     LMAQRSQLEAEIKEAQLKAKEEMMQGIQIAKEMAQQELSSQKAAYESKIKALEAELREE
                     SQRKKMQEINNQKANHKIEELEKAKQHLEQ"
                     /gene_synonym="MCPH20; MKS12"
                     /gene="KIF14"
                     /product="kinesin-like protein KIF14 isoform 1"
                     /codon_start=1
                     /db_xref="{'GI': '7661878', 'CCDS': 'CCDS30963.1',
                     'GeneID': '9928', 'HGNC': 'HGNC:19181', 'MIM': '611279'}"
                     /note="isoform 1 is encoded by transcript variant 1;
                     kinesin-like protein KIF14; epididymis secretory sperm
                     binding protein"
                     /note="color: #993366"
                     /protein_id="NP_055690.1"
                     /transl_table=1
                     /label="KIF14"
     CDS             4996..5856
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /product="β-lactamase"
                     /codon_start=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /transl_table=1
                     /label="AmpR"
     rep_origin      6027..6615
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      4154..4609
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   3939..4079
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     promoter        4891..4995
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          3817..3857
                     /label="Splicing Donor"
                     /note="color: noColor"
     misc_feature    3861..3894
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     CDS             727..753
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /codon_start=1
                     /transl_table=1
                     /label="HA"
                     /note="color: #cc99b2"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatctt cattacacag tactcataat
      781 agaaataaca gcggtgatat tcttgatatt ccttcttccc aaaatagttc atcactgaat
      841 gccctcaccc acagtagccg acttaagctg catttgaagt cggatatgtc agaatgtgaa
      901 aatgatgatc cattattgag atctgcaggt aaagtcagag acataaatag aacttatgtt
      961 atttctgcca gtagaaaaac agcagacatg ccccttaccc ctaatcctgt aggtagattg
     1021 gcacttcaga ggagaactac aaggaacaaa gaatcatctt tgcttgttag tgagttggaa
     1081 gacacaactg aaaaaacagc agaaacacgt cttacattac aacgtcgtgc taaaacagat
     1141 tctgcagaaa agtggaaaac agctgaaata gattctgtca aaatgacact gaatgtggga
     1201 ggtgaaacag aaaataatgg tgtttctaag gaaagtagaa caaatgtaag gattgtaaat
     1261 aatgctaaaa actcttttgt tgcctcttct gtacctttag atgaagatcc acaggtcatt
     1321 gaaatgatgg ctgataagaa atacaaagaa acattttctg cccccagtag agcaaatgaa
     1381 aatgttgcac ttaagtactc aagtaataga ccacccattg cttccctgag tcagactgaa
     1441 gttgttagat caggacactt gacaacgaaa cctactcaga gcaagttgga tatcaaagtg
     1501 ttgggaacag gaaacttgta tcatagaagt attgggaagg aaattgcaaa aacttcaaat
     1561 aaatttggga gcttagaaaa aagaacacct acaaaatgta caacagaaca caaactgaca
     1621 acaaagtgca gcctgcctca gcttaagagc ccagctccat caatactgaa gaatagaatg
     1681 tctaaccttc aagttaaaca aagaccaaaa agttcctttc ttgcaaataa acaggaaaga
     1741 tccgcagaaa atacaattct tcccgaagaa gaaactgtag ttcagaacac ctctgcagga
     1801 aaagacccct taaaagtaga gaatagtcaa gtgacagtgg cagtacgcgt aagacctttc
     1861 accaagagag agaagattga aaaagcatcc caggtagtct tcatgagtgg gaaagaaata
     1921 actgtggaac accctgacac gaaacaagtt tataatttta tttatgatgt ttcattctgg
     1981 tcttttgatg aatgtcatcc tcactacgct agccagacaa ctgtctatga gaagctagca
     2041 gcaccactcc tagaaagagc cttcgaaggc ttcaatacct gtctttttgc ttatggtcag
     2101 actggctctg gaaaatcata tacgatgatg ggatttagtg aagaaccagg aataattcca
     2161 agattttgtg aagatctttt ttctcaagta gccagaaaac aaacccaaga ggtcagctat
     2221 cacattgaaa tgagcttctt tgaagtatat aatgaaaaaa ttcacgacct tctggtttgt
     2281 aaagatgaaa atgggcagag aaagcaacca ctgagagtga gggaacatcc tgtttatgga
     2341 ccatatgttg aagcactgtc aatgaacatt gtcagttctt acgctgatat ccagagttgg
     2401 ctagaattgg gaaataaaca aagagctact gctgctactg gtatgaatga taaaagttcc
     2461 cgatctcatt cagttttcac cctggtgatg acccagacca agacagaatt tgtggaaggg
     2521 gaagaacacg atcacagaat aacaagtcga attaacctaa tagatctggc aggcagtgag
     2581 cgctgctcta cggctcacac taatggagat cgactaaagg aaggtgtgag tattaataag
     2641 tccttgctaa ctttgggaaa agttatatct gcactttcgg aacaagcaaa ccaaaggagt
     2701 gtttttattc cttatcgtga atctgttctt acatggctgt taaaagaaag tctgggtgga
     2761 aattcaaaaa ctgcaatgat tgctacgatt agtcccgctg ccagcaacat agaagaaaca
     2821 ttaagcacac ttagatatgc taaccaagcc cgtttaatag tcaacattgc taaagtaaat
     2881 gaagatatga acgctaagtt aattagagaa ttgaaggcag aaattgcaaa gctaaaagct
     2941 gctcagagaa acagtcggaa tattgaccct gaacgataca ggctctgtcg gcaagaaata
     3001 acatccttaa gaatgaaact gcatcaacag gagagagaca tggcagaaat gcaaagagtg
     3061 tggaaagaaa agtttgaaca agctgaaaaa agaaaacttc aagaaacaaa agagttacag
     3121 aaagcaggaa ttatgtttca aatggacaat catttaccaa accttgttaa tctgaatgaa
     3181 gatccacaac tatctgagat gctgctatat atgataaaag aaggaacaac tacagttgga
     3241 aagtataaac caaactcaag ccatgatatt cagttatctg gggtgctgat tgctgatgat
     3301 cattgtacta tcaaaaattt tggtgggaca gtgagtatta tcccagttgg ggaagcaaag
     3361 acatatgtaa atggaaaaca tattttggaa atcacagtat tacgtcatgg tgatcgagtg
     3421 attcttggtg gagatcatta ttttagattt aatcatccag tagaagtcca gaaaggaaaa
     3481 aggccatctg gaagagatac tcctataagt gagggtccaa aagactttga atttgcaaaa
     3541 aatgagttgc tcatggcaca gagatcacaa cttgaagcag aaataaaaga ggctcagttg
     3601 aaggcaaagg aagaaatgat gcaaggaatc cagattgcaa aagaaatggc tcagcaagag
     3661 ctttcttctc aaaaagctgc atatgaaagc aaaataaaag cactggaagc agaactgaga
     3721 gaagagtctc aaaggaaaaa aatgcaggaa ataaataacc agaaggctaa tcacaaaatt
     3781 gaggaattag aaaaggcaaa gcagcatctt gaacaggtaa gtatcaaggt tacaagacag
     3841 gtttaaggag accaataagc tattattcgt ataggatact ttatacgaag ttattaagtt
     3901 atggagaccg gtgtggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt
     3961 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     4021 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     4081 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     4141 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4201 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4261 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4321 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     4381 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4441 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat
     4501 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4561 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     4621 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     4681 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     4741 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     4801 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     4861 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4921 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4981 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5041 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5101 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5161 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5221 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     5281 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5341 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5401 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5461 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5521 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     5581 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5641 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     5701 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5761 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5821 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5881 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5941 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6001 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6061 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6121 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     6181 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6241 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6301 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6361 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6421 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6481 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6541 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6601 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6661 ccttttgctc acatgt
//
