LOCUS       .                       7087 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             415..4227
                     /gene="KIF1A"
                     /note="isoform 1 is encoded by transcript variant 1;
                     kinesin, heavy chain, member 1A, homolog of mouse;
                     kinesin-like protein KIF1A; microtubule-based motor KIF1A;
                     unc-104- and KIF1A-related protein; axonal transporter of
                     synaptic vesicles"
                     /note="color: #993366"
                     /translation="AGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKET
                     PKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSY
                     TMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKG
                     NLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNI
                     IFTQKRHDAETNITTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVI
                     SALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDET
                     LSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTNTVPGG
                     PKLTNALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNET
                     WEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLY
                     YIKDGITRVGREDGERRQDIVLSGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVN
                     GKKVTEPSILRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELL
                     EKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEV
                     NEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKK
                     KVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEK
                     LRQRLDLMREMYDRAAEVPSSVIEDCDNVVTGGDPFYDRFPWFRLVGSSAISGCNSYPL
                     LNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEDEEEEDLEDDVFPE
                     HALCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAIVSEKGEVKGFLRVAVQA
                     ISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQSESCPVVGMSRSGTSQEELRIVEGQG
                     QGADVGPSADEVNNNTCSAVPPEGLLLDSSEKAALDGPLDAALDHLRLGNTFTFRVTVL
                     QASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTKSFIEY
                     IKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPC
                     PGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQ"
                     /product="kinesin-like protein KIF1A isoform 1"
                     /transl_table=1
                     /codon_start=1
                     /gene_synonym="ATSV; C2orf20; HSN2C; MRD9; NESCAVS; SPG30;
                     UNC104"
                     /protein_id="NP_001230937.1"
                     /db_xref="{'GI': '345842524', 'CCDS': 'CCDS58757.1',
                     'GeneID': '547', 'HGNC': 'HGNC:888', 'MIM': '601255'}"
                     /label="KIF1A"
     CDS             5407..6267
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /product="β-lactamase"
                     /transl_table=1
                     /codon_start=1
                     /label="AmpR"
     rep_origin      6438..7026
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     rep_origin      4565..5020
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     promoter        156..367
                     /note="core promoter for human elongation factor EF-1α"
                     /note="color: #ffffff"
                     /label="EF-1α core promoter"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   4350..4490
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     promoter        5302..5406
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          4228..4268
                     /label="Splicing Donor"
                     /note="color: noColor"
     misc_feature    4272..4305
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     CDS             382..408
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /codon_start=1
                     /label="HA"
                     /note="color: #cc99b2"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgggca gagcgcacat cgcccacagt
      181 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg
      241 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
      301 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
      361 aacacagcgt acgccaccat gtacccttat gacgtacctg actatgctgg atctgccggg
      421 gcttcggtga aggtggcggt gcgggtccgc cccttcaatt cccgggaaat gagccgtgac
      481 tccaagtgca tcattcagat gtctggaagc accaccacca ttgttaaccc caaacagccc
      541 aaggagacgc ccaaaagctt cagctttgac tactcctact ggtcgcacac ctcacctgag
      601 gacatcaact acgcgtcgca gaagcaggtg taccgggaca tcggcgagga gatgctgcag
      661 catgcctttg agggatacaa cgtgtgcatc ttcgcctatg ggcagacggg tgccggcaag
      721 tcctacacca tgatgggcaa gcaggagaag gaccagcagg gcatcatccc acagctctgc
      781 gaggacctct tctctcggat caacgacacg accaacgaca acatgtccta ctccgtggag
      841 gtcagctaca tggagattta ctgtgagcgc gtccgtgacc tcctgaaccc caagaacaag
      901 ggcaaccttc gcgtgaggga gcacccactg ctggggccct acgtggagga cctctccaag
      961 ctggctgtca cctcctacaa tgacatccag gacctcatgg actcagggaa caaggccagg
     1021 accgtggcgg ccaccaacat gaatgagacc agcagtcgct cccacgccgt cttcaacatc
     1081 atcttcaccc agaagcgcca tgacgcagag accaatatca ccacggagaa ggtgagcaaa
     1141 atcagcctgg tggacctggc tgggagcgag cgggctgact ccacgggagc caagggcacg
     1201 cgcctcaagg agggggccaa catcaacaag tcgctgacca ccctgggcaa ggtcatctcc
     1261 gccctggctg aaatggactc cggacccaac aagaacaaga aaaagaagaa gacagatttc
     1321 attccgtacc gagattccgt gttgacctgg ctcctccggg aaaacctggg cggtaactca
     1381 aggacagcta tggtggcagc cctgagtcct gcagacatca actacgatga gacccttagc
     1441 acgctgaggt atgctgaccg ggccaagcag atccgctgca atgctgtcat caatgaggac
     1501 cccaacaaca agctgatccg cgagctgaag gatgaggtga cccggctgcg ggaccttctg
     1561 tacgcccagg gtcttggcga catcactgac accaacactg tgcctggagg acccaaattg
     1621 accaatgccc tggtgggtat gagcccctca tcctcgctct cagccctgtc cagccgcgcg
     1681 gcctccgtgt ccagcctcca cgagcgcatc ttgtttgccc cgggcagcga ggaggccatt
     1741 gaaagactga aggaaacaga gaagatcata gctgagctca atgagacctg ggaggagaag
     1801 ctgcggcgga cagaagccat ccggatggag agggaagccc tgctggccga gatgggtgtg
     1861 gccatgaggg aggatggcgg caccttgggc gtattctctc ccaaaaagac accacacctc
     1921 gtcaacctga acgaggaccc gctgatgtct gagtgcctgc tctactacat caaggatggg
     1981 atcaccagag tgggcaggga ggatggcgag aggcggcagg acattgttct gagtgggcac
     2041 ttcatcaagg aggagcactg cgtcttccgg agcgactcca ggggaggcag cgaagctgtg
     2101 gtgaccttgg agccctgtga gggggcagac acctacgtca atggcaagaa agtcacagag
     2161 cccagcatcc tgcgttcagg aaaccgcatc atcatgggta agagccatgt gttccggttc
     2221 aaccaccccg agcaggcccg gcaggagcgt gagcgcacgc cttgtgcgga gacgccagct
     2281 gagcctgtgg actgggcctt cgcccagcgt gagctgctgg agaagcaggg catcgacatg
     2341 aagcaggaga tggagcagag gctccaggaa ctggaggacc agtaccgccg cgagcgggag
     2401 gaggccacct acctgctgga gcagcagcgg ctggactatg agagcaagct ggaggctctg
     2461 cagaagcaga tggactccag gtactacccg gaggtgaacg aggaggagga ggagcccgag
     2521 gatgaagtcc agtggacaga gcgggagtgt gagctggcgc tctgggcctt ccggaagtgg
     2581 aagtggtacc agttcacgtc tctgcgggac ctgctgtggg gcaacgccat cttcctcaag
     2641 gaggccaatg ccatcagcgt ggagctgaaa aagaaggtac aattccagtt tgtcctcctg
     2701 acggacacac tctactcccc tctgccaccc gacctgctgc ccccagaggc cgccaaagac
     2761 cgagagacgc ggcccttccc ccgcaccatt gtggccgtgg aggtccagga ccagaagaac
     2821 ggggccaccc actactggac gctggagaag ctcaggcagc gtctggacct gatgcgggag
     2881 atgtacgacc gcgctgcaga ggtgccctcc agtgtcatcg aggactgtga caacgtggtg
     2941 accggcggag accccttcta tgaccgcttc ccctggttcc ggctggtggg cagttcagcc
     3001 atctctggct gcaacagcta ccctcttctc aacacatgca tgagcgagcg catggctgct
     3061 ctcaccccct cccccacctt ctcgagcccc gactccgacg ccaccgagcc tgccgaggag
     3121 cagagcgtgg gggaggagga ggaggaggag gaggaggagg aggatgagga ggaggaggac
     3181 ctggaggacg acgtctttcc ggagcacgcg ctgtgcgacg gccgggaccc gttttacgac
     3241 cggccccccc tgttcagttt agtaggaagg gccttcgtgt acctgagcaa cctgctgtac
     3301 cccgttcccc tggtacaccg tgtggcaatc gtcagcgaga agggcgaggt gaagggcttc
     3361 ctccgcgtgg ccgtccaggc catctcagcc gatgaagagg cccctgatta tggctctggc
     3421 gtccgccagt cgggaactgc taaaatctcc tttgatgacc agcattttga aaagttccag
     3481 tccgagtctt gccccgtggt ggggatgtcc cgctcgggaa cctcccagga agagcttcgc
     3541 atcgtggagg gccagggcca gggtgcagac gtggggccct cagccgatga agtcaacaac
     3601 aacacctgtt cagcagtgcc cccagaaggc ctcctcctag acagctctga gaaagccgcc
     3661 ctggatgggc ccctggatgc tgccctggac cacctccgcc tgggcaacac cttcaccttc
     3721 cgtgtgacag tcctgcaggc gtccagcatc tctgccgaat atgccgacat cttctgccag
     3781 ttcaacttca tccaccgcca cgacgaggcc ttctccacag agcccctgaa gaacacaggc
     3841 agaggccccc cacttggctt ctaccacgtc cagaacatcg cagtggaggt gaccaagtcc
     3901 ttcattgagt acatcaagag ccagcccatt gttttcgagg tctttggcca ctaccagcag
     3961 cacccgttcc cgcccctctg caaggacgtg ctcagccccc tgaggccctc gcgccgccac
     4021 ttccctcggg tcatgccact gtccaagcca gtgcccgcca ccaagctcag cacactgacg
     4081 cggccctgtc cgggaccctg ccactgcaag tacgacctgc tggtctactt cgagatctgt
     4141 gagctggagg ccaacggcga ttacatcccg gccgtggtgg accaccgtgg gggcatgcca
     4201 tgcatgggga ccttcctcct ccaccaggta agtatcaagg ttacaagaca ggtttaagga
     4261 gaccaataag ctattattcg tataggatac tttatacgaa gttattaagt tatggagacc
     4321 ggtgtggcac gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc
     4381 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     4441 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
     4501 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     4561 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4621 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4681 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4741 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     4801 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4861 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat
     4921 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4981 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     5041 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     5101 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     5161 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     5221 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     5281 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     5341 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5401 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5461 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5521 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5581 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5641 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5701 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5761 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5821 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5881 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5941 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     6001 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     6061 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     6121 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6181 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6241 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6301 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6361 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6421 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6481 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6541 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     6601 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6661 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6721 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6781 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6841 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6901 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6961 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7021 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     7081 cacatgt
//
