LOCUS       .                       6348 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1493..3049
                     /gene="KIF1A"
                     /product="kinesin-like protein KIF1A isoform 1"
                     /translation="GIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPN
                     ILSLNILSSGYIHPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEME
                     NCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVAD
                     AGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRH
                     YLLLREKLETAQRPVPEALSPAFSEDSESHGSSSASSPLSAEGRPSPLEAPNERQRELA
                     VKCLRLLTHTFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGVATLTPSSTCPSL
                     VEGRYGATDLRTPQPCSRPASPEPELLPEADSKKLPSPARATETDKEPQRLLVPDIQEI
                     RVSPIVSKKGYLHFLEPHTSGWARRFVVVRRPYAYMYNSDKDTVERFVLNLATAQVEYS
                     EDQQAMLKTPNTFAVCTEHRGILLQAASDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQM
                     RV"
                     /note="isoform 1 is encoded by transcript variant 1;
                     kinesin, heavy chain, member 1A, homolog of mouse;
                     kinesin-like protein KIF1A; microtubule-based motor KIF1A;
                     unc-104- and KIF1A-related protein; axonal transporter of
                     synaptic vesicles"
                     /note="color: #993366"
                     /db_xref="{'GI': '345842524', 'CCDS': 'CCDS58757.1',
                     'GeneID': '547', 'HGNC': 'HGNC:888', 'MIM': '601255'}"
                     /gene_synonym="ATSV; C2orf20; HSN2C; MRD9; NESCAVS; SPG30;
                     UNC104"
                     /codon_start=1
                     /protein_id="NP_001230937.1"
                     /transl_table=1
                     /label="KIF1A"
     CDS             288..1318
                     /product="site-specific recombinase"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /codon_start=1
                     /protein_id=""
                     /transl_table=1
                     /label="Cre"
     CDS             4668..5528
                     /gene="bla"
                     /product="β-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /codon_start=1
                     /transl_table=1
                     /label="AmpR"
     rep_origin      5699..6287
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     rep_origin      3826..4281
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     terminator      3137..3568
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   3611..3751
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     promoter        4563..4667
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     CDS             3056..3121
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /codon_start=1
                     /transl_table=1
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     protein_bind    1367..1400
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /label="lox2272-JTZ17"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac agggcatcca
     1501 gcgacggatt acggtgacac tactgcatga gacaggcagc catatccgct ggaaggaagt
     1561 gcgcgagctg gtcgtgggcc gcatccgaaa cactccagag accgacgagt ccctgatcga
     1621 ccccaacatc ttgtctctca acatcctctc ttccggatac atccacccag cccaagatga
     1681 ccggaccttt taccaatttg aggctgcgtg ggacagctcc atgcacaact ctctcctgct
     1741 gaaccgggtc accccttatc gagagaaaat ctacatgaca ctctccgctt atatcgagat
     1801 ggagaactgc acccagccgg ctgttgtcac caaggacttc tgcatggtct tctattcccg
     1861 tgatgccaag ctgccagcct cgcgctccat ccgcaacctc tttggcagtg ggagccttcg
     1921 ggcctcagag agtaaccgtg tgactggtgt gtacgagctc agcctgtgcc acgtggctga
     1981 cgcgggcagc ccagggatgc agcgccggcg ccgacgagtc ctggacacat ctgtggccta
     2041 tgtccggggc gaggagaacc tggcaggctg gaggccccgg agtgacagtc tcattctgga
     2101 ccaccagtgg gagctggaga agctgagcct cctgcaggag gtggagaaga ctaggcacta
     2161 cctgctcctg cgggagaagc tggagaccgc ccagcggcct gtcccggagg cactgtcccc
     2221 ggccttcagc gaggactctg agtcccatgg ctcctccagc gcctcctccc cgctctcggc
     2281 tgagggccgc ccatcacccc tggaggctcc caacgagagg cagcgggagc tggccgtcaa
     2341 gtgcttgcgc ctgctcacgc acacattcaa cagagagtac acacacagcc acgtctgcgt
     2401 cagtgccagc gagagcaagc tctccgagat gtctgtcacc ctgctccggg acccgtcgat
     2461 gtcccctcta ggggtggcca ctctcacccc ctcctccact tgcccctctc tggttgaagg
     2521 gcggtacggt gccactgacc tgaggacccc gcagccctgc tcccggccag ccagcccaga
     2581 gcccgagctg ctgccagagg ccgactccaa gaagctccct tcccctgccc gggcaacaga
     2641 gacagacaag gagccccagc gcctgctggt ccctgacatc caggagatcc gagtcagccc
     2701 gatcgtttcc aagaaggggt acctgcactt cctggagccg cacacgtcag gctgggccag
     2761 gcgcttcgtg gtggtgcggc gcccctatgc ctacatgtac aacagcgaca aggacaccgt
     2821 ggagcggttc gtgctcaacc tggccactgc ccaggtggag tacagtgagg accagcaggc
     2881 tatgctcaag acacccaaca cattcgcggt gtgcacggaa caccgcggca tcctgctgca
     2941 ggccgccagc gacaaggaca tgcatgactg gctgtacgcc ttcaaccccc tcctggccgg
     3001 gaccatacgg tccaagctct ccagaaggag gtctgcccag atgcgggtct gtacagacta
     3061 caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa
     3121 gtaagaattc tgatgactcg agataatcaa cctctggatt acaaaatttg tgaaagattg
     3181 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3241 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3301 ttagttcttg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     3361 cggctgttgg gcactgacaa ttccgtggtg tttatttgtg aaatttgtga tgctattgct
     3421 ttatttgtaa ccatctagct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     3481 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     3541 tcagggggag atgtgggagg ttttttaaag atctggagac cggtgtggca cgtgcggacc
     3601 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     3661 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     3721 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
     3781 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
     3841 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     3901 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     3961 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4021 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     4081 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4141 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga
     4201 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     4261 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
     4321 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     4381 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     4441 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     4501 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     4561 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4621 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4681 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4741 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4801 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     4861 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     4921 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     4981 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5041 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5101 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5161 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5221 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5281 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5341 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5401 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5461 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5521 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     5581 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5641 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5701 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5761 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5821 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     5881 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5941 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6001 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6061 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6121 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6181 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6241 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6301 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
