LOCUS       .                       6601 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             760..3741
                     /translation="MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSW
                     EQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQVI
                     MADSEICGTKDPTFHRILLDTRFELPLDIPEEEARYWAKKLEQLNAMRDQDEYSFQDEQ
                     DKPLPVPSNQCCNWNYFGWGEQHNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNAS
                     VHQYSVRPPPLGSRESYSDSMHSYEEFSEPQALSPTGSSRYASSGELSQGSSQLSEDFD
                     PDEHSLQGSDMEDERDRDSYHSCHSSVSYHKDSPRWDQDEEELEEDLEDFLEEEELPED
                     EEELEEEEEEVPDDLGSYAQREDVAVAEPKDFKRISLPPAAPGKEDKAPVAPTEAPDMA
                     KVAPKPATPDKVPAAEQIPEAEPPKDEESFRPREDEEGQEGQDSMSRAKANWLRAFNKV
                     RMQLQEARGEGEMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAG
                     ITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWG
                     IARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRE
                     RNKPEIFELIQEIFAVTKTAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKT
                     GSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRV
                     KQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKV
                     APYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGV
                     ESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGK
                     ERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSP
                     PRASQ"
                     /gene_synonym="Munc13-1"
                     /gene="UNC13A"
                     /product="protein unc-13 homolog A isoform 1"
                     /codon_start=1
                     /db_xref="{'GI': '283837842', 'CCDS': 'CCDS46013.2',
                     'GeneID': '23025', 'HGNC': 'HGNC:23150', 'MIM': '609894'}"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein unc-13 homolog A"
                     /note="color: #993366"
                     /protein_id="NP_001073890.2"
                     /transl_table=1
                     /label="UNC13A"
     CDS             4921..5781
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /gene="bla"
                     /product="β-lactamase"
                     /codon_start=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /transl_table=1
                     /label="AmpR"
     rep_origin      5952..6540
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      4079..4534
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /direction=RIGHT
                     /label="f1 ori"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   3864..4004
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     promoter        4816..4920
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          3742..3782
                     /label="Splicing Donor"
                     /note="color: noColor"
     misc_feature    3786..3819
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     CDS             727..753
                     /translation="YPYDVPDYA"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /codon_start=1
                     /transl_table=1
                     /label="HA"
                     /note="color: #cc99b2"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatcta tgtctctgct ttgcgttgga
      781 gtcaaaaaag ccaagtttga tggtgcccaa gagaaattca acacgtacgt gaccctgaaa
      841 gtgcagaatg tcaagagcac gaccatcgcg gtgcggggca gccagcccag ctgggagcag
      901 gatttcatgt tcgagattaa ccgtctggat ttgggactga cggtggaggt gtggaataag
      961 ggtctcatct gggacacaat ggtgggcact gtgtggatcc cactgaggac catccgccag
     1021 tccaatgagg agggccctgg agagtggctg acgctggact cccaggtcat catggcagac
     1081 agtgagatct gtggcaccaa ggaccccacc ttccaccgca tcctcctgga cacgcgcttt
     1141 gagctaccct tagacattcc tgaagaggag gctcgctact gggccaagaa gctggagcag
     1201 ctcaatgcta tgcgggacca ggatgaatat tcgttccaag atgagcaaga caagcctctg
     1261 cctgtcccca gcaaccagtg ctgcaactgg aattattttg gctggggtga gcagcacaac
     1321 gatgaccccg acagtgcagt ggatgatcgt gacagtgact accgcagtga aacgagcaac
     1381 agcatcccgc cgccctatta tactacgtca caacccaacg cctcagtcca ccaatattct
     1441 gttcgcccac cacccctggg ctcccgggag tcctacagtg actccatgca cagttacgag
     1501 gagttctctg agccacaagc cctcagcccc acgggtagca gccgctatgc ctcttccggg
     1561 gagctgagcc agggaagctc tcagctgagc gaggacttcg accctgacga gcacagcctg
     1621 cagggctccg acatggagga tgagcgggac cgggactcct accactcctg ccacagctcg
     1681 gtcagctacc acaaagactc gcctcgctgg gaccaggatg aggaagagct ggaggaggac
     1741 ctggaggact tcctggagga ggaggagctg cctgaagatg aggaggagct ggaggaggag
     1801 gaggaggagg tgcctgacga tttgggcagc tatgcccagc gtgaagacgt agctgtggct
     1861 gagcccaaag acttcaaacg catcagcctc ccgccagctg ccccagggaa ggaggacaag
     1921 gccccagtgg cacccaccga ggcccccgac atggccaagg tggcccccaa gccagccacg
     1981 cccgacaagg tgcctgcagc tgagcagatc cctgaggctg agccacccaa ggacgaggag
     2041 agtttcaggc cgagagagga tgaggaaggc caggaggggc aggactccat gtccagggcc
     2101 aaggccaact ggctgcgtgc cttcaacaag gtgcggatgc agctgcagga ggcccgggga
     2161 gaaggagaga tgtctaaatc cctatggttc aaaggcggcc cagggggcgg tctcatcatc
     2221 atcgacagca tgccagacat ccgcaagagg aaacctatcc cactcgtgag cgacttggcc
     2281 atgtccctgg tccagtccag gaaagcgggc atcacctcgg ccttggcctc cagcacgttg
     2341 aacaacgagg agctgaaaaa ccacgtttac aagaagaccc tgcaagcctt aatctacccc
     2401 atctcgtgca cgacgccaca caacttcgaa gtgtggacgg ccaccacgcc cacctactgc
     2461 tacgagtgcg aggggctgct gtggggcatc gcgaggcagg gcatgcgctg caccgagtgc
     2521 ggtgtcaagt gccacgagaa gtgccaggac ctgctcaacg ccgactgcct gcagcgggct
     2581 gcggagaaga gctccaagca cggggcggag gaccggacac agaacatcat catggtgctc
     2641 aaggaccgca tgaagatccg ggagcgcaac aagcccgaga tcttcgagct catccaggag
     2701 atcttcgcgg tgaccaagac ggcgcacacg cagcagatga aggcggtcaa gcagagcgtg
     2761 ctggacggca cgtccaagtg gtccgccaag atcagcatca ccgtggtctg cgcccagggc
     2821 ttgcaggcaa aggacaagac aggatccagt gacccctatg tcaccgtcca ggtcgggaag
     2881 accaagaaac ggacaaaaac catctatggg aacctcaacc cggtgtggga ggagaatttc
     2941 cactttgaat gtcacaattc ctccgaccgc atcaaggtgc gcgtctggga cgaggatgac
     3001 gacatcaaat cccgcgtgaa acagaggttc aagagggaat ctgacgattt cctggggcag
     3061 acgatcattg aggtgcggac gctcagcggc gagatggacg tgtggtacaa cctggacaag
     3121 cgaactgaca aatctgccgt gtcgggtgcc atccggctcc acatcagtgt ggagatcaaa
     3181 ggcgaggaga aggtggcccc gtaccatgtc cagtacacct gtctgcatga gaacctgttc
     3241 cacttcgtga ccgacgtgca gaacaatggg gtcgtgaaga tcccagatgc caagggtgac
     3301 gatgcctgga aggtttacta cgatgagaca gcccaggaga ttgtggacga gtttgccatg
     3361 cgctacggcg tcgagtccat ctaccaagcc atgacccact ttgcctgcct ctcctccaag
     3421 tatatgtgcc caggggtgcc tgccgtcatg agcaccctgc tcgccaacat caatgcctac
     3481 tacgcacaca ccaccgcctc caccaacgtg tctgcctccg accgcttcgc cgcctccaac
     3541 tttgggaaag agcgcttcgt gaaactcctg gaccagctgc ataactccct gcggattgac
     3601 ctctccatgt accggaataa cttcccagcc agcagcccgg agagactcca ggacctcaaa
     3661 tccactgtgg accttctcac cagcatcacc ttctttcgga tgaaggtaca agaactccag
     3721 agcccgcccc gagccagcca ggtaagtatc aaggttacaa gacaggttta aggagaccaa
     3781 taagctatta ttcgtatagg atactttata cgaagttatt aagttatgga gaccggtgtg
     3841 gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     3901 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     3961 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     4021 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     4081 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4141 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4201 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4261 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     4321 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4381 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
     4441 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4501 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     4561 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     4621 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     4681 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     4741 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     4801 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4861 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4921 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4981 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5041 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5101 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5161 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5221 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5281 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5341 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5401 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5461 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5521 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5581 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5641 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5701 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5761 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5821 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5881 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5941 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6001 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6061 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     6121 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6181 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6241 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6301 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6361 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6421 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6481 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6541 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6601 t
//
