LOCUS       .                       7058 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1493..3771
                     /db_xref="{'GI': '68299795', 'CCDS': 'CCDS34204.1',
                     'GeneID': '1105', 'HGNC': 'HGNC:1915', 'MIM': '602118'}"
                     /gene="CHD1"
                     /codon_start=1
                     /transl_table=1
                     /EC_number="3.6.4.12"
                     /product="chromodomain-helicase-DNA-binding protein 1
                     isoform 2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     ATP-dependent helicase CHD1"
                     /note="color: #993366"
                     /gene_synonym="CHD-1; PILBOS"
                     /translation="SAPSSSTPFNKEELSAILKFGAEELFKEPEGEEQEPQEMDIDEIL
                     KRAETHENEPGPLTVGDELLSQFKVANFSNMDEDDIELEPERNSKNWEEIIPEDQRRRL
                     EEEERQKELEEIYMLPRMRNCAKQISFNGSEGRRSRSRRYSGSDSDSISEGKRPKKRGR
                     PRTIPRENIKGFSDAEIRRFIKSYKKFGGPLERLDAIARDAELVDKSETDLRRLGELVH
                     NGCIKALKDSSSGTERTGGRLGKVKGPTFRISGVQVNAKLVISHEEELIPLHKSIPSDP
                     EERKQYTIPCHTKAAHFDIDWGKEDDSNLLIGIYEYGYGSWEMIKMDPDLSLTHKILPD
                     DPDKKPQAKQLQTRADYLIKLLSRDLAKKEALSGAGSSKRRKARAKKNKAMKSIKVKEE
                     IKSDSSPLPSEKSDEDDDKLSESKSDGRERSKKSSVSDAPVHITASGEPVPISEESEEL
                     DQKTFSICKERMRPVKAALKQLDRPEKGLSEREQLEHTRQCLIKIGDHITECLKEYTNP
                     EQIKQWRKNLWIFVSKFTEFDARKLHKLYKHAIKKRQESQQNSDQNSNLNPHVIRNPDV
                     ERLKENTNHDDSSRDSYSSDRHLTQYHDHHKDRHQGDSYKKSDSRKRPYSSFSNGKDHR
                     DWDHYKQDSRYYSDREKHRKLDDHRSRDHRSNLEGSLKDRSHSDHRSHSDHRLHSDHRS
                     SSEYTHHKSSRDYRYHSDWQMDHRASSSGPRSPLDQRSPYGSRSPFEHSVEHKSTPEHT
                     WSSRKT"
                     /protein_id="NP_001261.2"
                     /label="CHD1"
     CDS             288..1318
                     /codon_start=1
                     /transl_table=1
                     /product="site-specific recombinase"
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /protein_id=""
                     /label="Cre"
     CDS             5378..6238
                     /gene="bla"
                     /codon_start=1
                     /transl_table=1
                     /product="β-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      6409..6997
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     rep_origin      4536..4991
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     terminator      3847..4278
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   4321..4461
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     promoter        5273..5377
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     CDS             3778..3843
                     /codon_start=1
                     /transl_table=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     protein_bind    1367..1400
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /label="lox2272-JTZ17"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggttctgcc
     1501 ccatcaagtt ctactccttt caataaagaa gagttatcag ccattttaaa gtttggtgct
     1561 gaagaacttt ttaaggaacc tgaaggagaa gaacaagagc cccaggaaat ggatatagat
     1621 gaaatcttga agagagctga aactcatgaa aatgaaccag gtcctttaac tgtaggagat
     1681 gaattgcttt cccagttcaa ggttgccaac ttctcaaata tggatgagga tgacattgag
     1741 ttggaacctg aaagaaattc aaagaattgg gaggaaatta ttccagaaga tcaaagaaga
     1801 cgattagaag aagaagaaag acaaaaggaa cttgaagaaa tttatatgct cccaagaatg
     1861 agaaattgtg caaaacagat tagtttcaat ggaagtgaag ggaggcgcag tagaagtagg
     1921 agatactctg gatctgatag tgattccatc tcagaaggga aaaggccaaa gaaacgtgga
     1981 agaccacgga ctattcctcg ggagaatatt aaaggattta gtgatgcaga aattaggcgg
     2041 tttatcaaga gctataagaa atttggtggt cctctggaaa gattagatgc aattgctcga
     2101 gatgctgagt tagttgataa gtcagaaaca gaccttagac gactgggaga attggtacat
     2161 aatggttgca ttaaagcatt aaaggatagt tcttcaggaa cagaacgaac aggtggtaga
     2221 ctcggaaaag tgaagggtcc aacattccga atatcaggag tacaggtgaa tgccaaacta
     2281 gtcatctccc atgaagaaga attaatacct ttgcacaaat ccattccttc tgatccagaa
     2341 gaaagaaagc agtatactat cccatgccac acaaaggcag ctcattttga tatagactgg
     2401 ggcaaagaag atgattccaa tttgttaatt ggcatctatg aatatggata tggaagctgg
     2461 gaaatgatta aaatggatcc tgacctcagt ctaacacaca agattcttcc agatgatccc
     2521 gataaaaaac cacaagcaaa acagttgcag acccgtgcag actacctcat caaattactt
     2581 agtagagatc ttgcaaaaaa agaagctctt tctggtgcgg gaagttcaaa gaggagaaaa
     2641 gcaagagcta agaagaataa agcaatgaag tctataaaag tgaaagagga aataaagagt
     2701 gattcttctc ctctgccttc agagaagtct gatgaagatg atgataaatt gagtgaatcc
     2761 aagtctgatg gtagggaaag atccaagaaa tcttcagtgt cagatgctcc agttcatatc
     2821 acggcaagtg gtgaaccagt tcccatttct gaagaatctg aagagctgga tcagaagaca
     2881 ttcagcattt gtaaagaaag aatgaggcct gttaaagcag ctttgaaaca acttgatagg
     2941 cctgagaaag gcctttcaga aagagaacaa ctagagcata ctagacaatg tttaataaaa
     3001 attggagacc atatcacaga atgtctaaaa gagtatacaa atcctgaaca aattaagcaa
     3061 tggagaaaaa acctgtggat ttttgtatct aagtttactg aatttgatgc aagaaaatta
     3121 cataaattat ataagcatgc tattaaaaaa cggcaggagt ctcagcaaaa cagtgatcaa
     3181 aacagcaact tgaatcctca cgtgattaga aatccagatg tggaaagatt aaaagagaat
     3241 acaaatcacg atgatagcag cagggacagt tattcctctg atagacactt aactcagtac
     3301 catgatcatc ataaagaccg acatcaggga gattcttaca aaaaaagtga ttccaggaaa
     3361 agaccctatt cttcttttag taatggtaaa gaccatcgtg attgggatca ctacaagcaa
     3421 gacagcagat attacagtga cagagagaaa cacagaaaac tggatgatca caggagtaga
     3481 gatcacaggt caaatttgga aggaagttta aaagatagat ctcattctga tcatcgttct
     3541 cactcagatc atcggttaca ttcagaccac cggtcaagtt ctgaatatac gcaccataaa
     3601 tcttccaggg attataggta tcactcagac tggcaaatgg accacagagc ttccagcagt
     3661 ggccctaggt caccactaga tcagagatct ccttatggct ccagatctcc atttgaacat
     3721 tcagttgaac acaaaagtac accggagcat acctggagta gtcggaaaac atgtacagac
     3781 tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac
     3841 aagtaactcg agataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     3901 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     3961 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttagttcttg
     4021 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     4081 gcactgacaa ttccgtggtg tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     4141 ccatctagct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     4201 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     4261 atgtgggagg ttttttaaag atctggagac cggtgtggca cgtgcggacc gagcggccgc
     4321 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
     4381 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
     4441 gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg
     4501 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
     4561 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4621 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4681 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4741 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4801 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4861 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggccta
     4921 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     4981 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     5041 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     5101 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     5161 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     5221 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     5281 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5341 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     5401 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5461 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5521 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5581 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     5641 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     5701 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     5761 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     5821 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     5881 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     5941 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6001 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6061 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6121 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6181 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6241 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6301 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6361 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6421 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6481 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6541 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6601 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6661 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6721 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6781 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     6841 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6901 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6961 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7021 acggttcctg gccttttgct ggccttttgc tcacatgt
//
