LOCUS       .                       6817 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             727..753
                     /codon_start=1
                     /transl_table=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
                     /label="HA"
                     /note="color: #cc99b2"
     misc_feature    4002..4035
                     /label="lox2272-JT1571"
                     /note="color: #ff6600"
     intron          3958..3998
                     /label="Splicing Donor"
                     /note="color: noColor"
     promoter        5032..5136
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   4080..4220
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     rep_origin      4295..4750
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     promoter        169..711
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
                     /note="color: #ffffff"
                     /label="EF1α"
     rep_origin      6168..6756
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             5137..5997
                     /gene="bla"
                     /codon_start=1
                     /transl_table=1
                     /product="β-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     CDS             760..3957
                     /db_xref="{'GI': '153945715', 'CCDS': 'CCDS42036.1',
                     'GeneID': '55930', 'HGNC': 'HGNC:7604', 'MIM': '610022'}"
                     /gene="MYO5C"
                     /codon_start=1
                     /transl_table=1
                     /product="unconventional myosin-Vc"
                     /note="myosin 5C; myosin-Vc"
                     /note="color: #993366"
                     /translation="AVAELYTQYNRVWIPDPEEVWKSAEIAKDYRVGDKVLRLLLEDGT
                     ELDYSVNPESLPPLRNPDILVGENDLTALSYLHEPAVLHNLRIRFAESKLIYTYSGIIL
                     VAMNPYKQLPIYGDAIIHAYSGQNMGDMDPHIFAVAEEAYKQMARNNRNQSIIVSGESG
                     AGKTVSARYAMRYFATVSKSGSNAHVEDKVLASNPITEAVGNAKTTRNDNSSRFGKYTE
                     ISFDEQNQIIGANMSTYLLEKSRVVFQSENERNYHIFYQLCASAQQSEFKHLKLGSAEE
                     FNYTRMGGNTVIEGVNDRAEMVETQKTFTLLGFKEDFQMDVFKILAAILHLGNVQITAV
                     GNERSSVSEDDSHLKVFCELLGLESGRVAQWLCNRKIVTSSETVVKPMTRPQAVNARDA
                     LAKKIYAHLFDFIVERINQALQFSGKQHTFIGVLDIYGFETFDVNSFEQFCINYANEKL
                     QQQFNMHVFKLEQEEYMKEDIPWTLIDFYDNQPVIDLIEAKMGILELLDEECLLPHGTD
                     ENWLQKLYNNFVNRNPLFEKPRMSNTSFVIQHFADKVEYKCEGFLEKNRDTVYDMLVEI
                     LRASKFHLCANFFQENPTPPSPFGSMITVKSAKQVIKPNSKHFRTTVGSKFRSSLYLLM
                     ETLNATTPHYVRCIKPNDEKLPFEFDSKRIVQQLRACGVLETIRISAQSYPSRWTYIEF
                     YSRYGILMTKQELSFSDKKEVCKVVLHRLIQDSNQYQFGKTKIFFRAGQVAYLEKLRLD
                     KLRQSCVMVQKHMRGWLQRKKFLRERRAALIIQQYFRGQQTVRKAITAVALKEAWAAII
                     IQKHCRGYLVRSLYQLIRMATITMQAYSRGFLARRRYRKMLEEHKAVILQKYARAWLAR
                     RRFQSIRRFVLNIQLTYRVQRLQKKLEDQNKENHGLVEKLTSLAALRAGDVEKIQKLEA
                     ELEKAATHRRNYEEKGKRYRDAVEEKLAKLQKHNSELETQKEQIQLKLQEKTEELKEKM
                     DNLTKQLFDDVQKEERQRMLLEKSFELKTQDYEKQIQSLKEEIKALKDEKMQLQHLVEG
                     EHVTSDGLKAEVARLSKQ"
                     /protein_id="NP_061198.2"
                     /label="MYO5C"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatctg cggtggccga gctgtacacg
      781 cagtacaaca gggtctggat tcccgatcct gaagaagttt ggaagtctgc tgaaatagcc
      841 aaggactaca gagttggtga caaggtcctg cgactcctgc tggaggatgg aacggagctg
      901 gattattctg tcaatccaga atctctgcct ccacttcgga atcctgacat cctcgtgggc
      961 gagaatgacc tcacggctct cagctatctt cacgagcccg cggtgctcca caacctcaga
     1021 atccgctttg cagaatccaa actcatttac acctacagtg gaatcatttt ggtggccatg
     1081 aatccttaca agcagttgcc aatatacgga gatgccatca tccacgccta cagcgggcag
     1141 aacatgggcg atatggaccc acacatattt gccgtggcag aagaggcata caagcagatg
     1201 gccagaaaca acagaaacca gtccataatt gtaagtgggg agtcaggtgc tggaaagaca
     1261 gtgtcggctc gctatgccat gaggtacttt gccaccgtca gcaaatcggg cagcaacgct
     1321 cacgtggaag acaaggtcct ggcatccaat cccatcaccg aggccgttgg aaatgccaag
     1381 accacccgca atgacaatag tagtcggttt gggaaataca cagaaatcag ttttgatgaa
     1441 caaaatcaaa ttataggagc caacatgagc acttacctcc tggagaaatc cagagttgtc
     1501 tttcaatcgg aaaatgaacg aaattaccac attttctatc agctttgtgc atctgcacag
     1561 cagtcggaat ttaaacatct taaattgggg agtgccgaag aatttaatta tacaagaatg
     1621 ggaggcaata ctgtcattga gggtgtgaat gatcgagctg aaatggtaga gactcaaaag
     1681 accttcacgc ttctgggttt caaggaggat tttcagatgg acgtttttaa aatcctggca
     1741 gccatcctac atctgggcaa tgtgcagatc accgcggtgg gcaacgagag gtcctcagtt
     1801 agtgaggatg acagtcacct gaaggtgttc tgtgagctcc tgggcctgga gagtggcaga
     1861 gttgctcagt ggctgtgcaa tcgcaaaatc gtcacaagct ctgagacggt ggtaaaaccc
     1921 atgaccaggc ctcaggctgt caacgccagg gatgcactgg ccaaaaagat ctatgctcac
     1981 ctgttcgact tcattgtgga gagaattaac caagcgttgc agttttcagg caagcagcac
     2041 acttttattg gtgttttgga catttatggt tttgaaacct ttgatgtgaa cagctttgaa
     2101 caattttgca tcaattacgc taatgaaaaa ctgcaacaac agtttaacat gcatgtcttc
     2161 aaactggaac aagaagaata catgaaggaa gatatacctt ggacgctgat agatttttat
     2221 gacaatcaac cagttattga cctgattgaa gcaaaaatgg gaattctgga gttactggat
     2281 gaagaatgtt tgttaccaca tggaactgat gaaaactggc ttcaaaagct gtataataat
     2341 tttgtcaaca ggaacccttt gtttgaaaag cctagaatgt caaacacatc ctttgtcatc
     2401 cagcactttg ctgataaggt agagtataaa tgtgaaggtt tcctggagaa aaacagagac
     2461 accgtctatg acatgctggt tgaaatcctg agagcaagca agtttcatct ctgtgccaac
     2521 ttttttcaag aaaatccaac tcctccttct ccttttggtt caatgattac agttaaatct
     2581 gcaaagcaag tcatcaagcc aaacagcaag catttccgga ccacagttgg gagcaagttc
     2641 cgcagctctc tgtacttgct catggagacc ctcaatgcga cgacgcccca ctacgttcga
     2701 tgcatcaagc caaatgatga gaagttaccc tttgaatttg actccaaaag aattgttcag
     2761 cagctgcgag cctgcggcgt tttagaaacg attcgcatta gtgcacagag ctacccttcc
     2821 aggtggacat acatcgagtt ctacagtcgc tacggcattc tcatgaccaa gcaagagctt
     2881 tccttcagcg ataaaaagga ggtgtgcaag gtggttttac acagactcat ccaggattct
     2941 aatcagtacc agtttggtaa aaccaaaatt ttcttcagag caggacaagt ggcttattta
     3001 gagaaacttc gattggataa actgaggcag agttgtgtta tggtacaaaa gcacatgcgt
     3061 ggctggctcc agaggaaaaa attcctccga gagagacgag ccgccctgat aatccagcag
     3121 tacttccggg gtcagcaaac tgtgaggaaa gctattactg cagtggcctt aaaagaagct
     3181 tgggcagcca taatcattca gaagcactgc cgcgggtatc ttgttcgcag cctgtatcag
     3241 ttgattcgca tggccaccat cacaatgcag gcctacagcc gaggattcct ggcaaggagg
     3301 aggtatcgaa agatgctgga ggaacataag gctgtgatcc tacagaaata cgcacgggcg
     3361 tggctggcca gacgcagatt ccagagtatc cgacgattcg tgcttaatat tcagcttact
     3421 tacagggtcc agcgtttgca gaaaaagctg gaagatcaga acaaagaaaa ccatgggctg
     3481 gtggagaagc tgactagcct ggctgctctt cgagctgggg atgtggaaaa gattcagaag
     3541 ctggaagcag aactagaaaa agcagccact cacaggcgaa attacgagga gaaggggaag
     3601 agatacaggg atgctgtgga agagaaattg gcaaagcttc agaagcataa ttcagaactg
     3661 gaaacacaga aagaacaaat acagctgaag cttcaagaga agactgaaga gttaaaagaa
     3721 aaaatggaca acctcaccaa gcagctcttt gatgatgtac aaaaggaaga acggcaaaga
     3781 atgcttcttg aaaaaagttt tgaactgaaa acacaagact atgagaagca gattcagtct
     3841 ttgaaagaag aaattaaagc tctcaaggat gagaagatgc aactccaaca cctggtggag
     3901 ggggagcacg tcacttctga tggcttgaag gcggaagtgg cccgcctgag caagcaggta
     3961 agtatcaagg ttacaagaca ggtttaagga gaccaataag ctattattcg tataggatac
     4021 tttatacgaa gttattaagt tatggagacc ggtgtggcac gtgcggaccg agcggccgca
     4081 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
     4141 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
     4201 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     4261 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     4321 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4381 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4441 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4501 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4561 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4621 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4681 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     4741 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     4801 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     4861 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4921 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     4981 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5041 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5101 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5161 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5221 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5281 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5341 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5401 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5461 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5521 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5581 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5641 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5701 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5761 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5821 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5881 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5941 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6001 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6061 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6121 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6181 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6241 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6301 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6361 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6421 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6481 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6541 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6601 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6661 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6721 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6781 cggttcctgg ccttttgctg gccttttgct cacatgt
//
