LOCUS       .                       6804 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    1367..1400
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /note="color: #31849b"
                     /label="lox2272-JTZ17"
     intron          1320..1360
                     /note="Splicing donor"
                     /note="color: noColor"
                     /label="Splicing Donor"
     CDS             3524..3589
                     /codon_start=1
                     /transl_table=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
                     /note="color: #cc99b2"
     intron          1401..1492
                     /note="Splicing acceptor"
                     /note="color: noColor"
                     /label="Splicing Acceptor"
     promoter        5019..5123
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     promoter        156..276
                     /label="pCALM1"
                     /note="color: #ffffff"
     repeat_region   4067..4207
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /rpt_type=inverted
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /label="AAV2 ITR"
     terminator      3593..4024
                     /note="Short WPRE and SV40 polyA"
                     /note="color: #ffffff"
                     /label="W3SL"
     rep_origin      4282..4737
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     rep_origin      6155..6743
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             5124..5984
                     /gene="bla"
                     /codon_start=1
                     /transl_table=1
                     /product="β-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     CDS             288..1318
                     /codon_start=1
                     /transl_table=1
                     /product="site-specific recombinase"
                     /note="Cre is a site-specific recombinase from
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /note="color: #993366"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
                     /protein_id=""
                     /label="Cre"
     CDS             1493..3517
                     /db_xref="{'GI': '153945715', 'CCDS': 'CCDS42036.1',
                     'GeneID': '55930', 'HGNC': 'HGNC:7604', 'MIM': '610022'}"
                     /gene="MYO5C"
                     /codon_start=1
                     /transl_table=1
                     /product="unconventional myosin-Vc"
                     /note="myosin 5C; myosin-Vc"
                     /note="color: #993366"
                     /translation="VKTISEFEKEIELLQAQKIDVEKHVQSQKREMREKMSEITKQLLE
                     SYDIEDVRSRLSVEDLEHLNEDGELWFAYEGLKKATRVLESHFQSQKDCYEKEIEALNF
                     KVVHLSQEINHLQKLFREENDINESIRHEVTRLTSENMMIPDFKQQISELEKQKQDLEI
                     RLNEQAEKMKGKLEELSNQLHRSQEEEGTQRKALEAQNEIHTKEKEKLIDKIQEMQEAS
                     DHLKKQFETESEVKCNFRQEASRLTLENRDLEEELDMKDRVIKKLQDQVKTLSKTIGKA
                     NDVHSSSGPKEYLGMLQYKREDEAKLIQNLILDLKPRGVVVNMIPGLPAHILFMCVRYA
                     DSLNDANMLKSLMNSTINGIKQVVKEHLEDFEMLSFWLSNTCHFLNCLKQYSGEEEFMK
                     HNSPQQNKNCLNNFDLSEYRQILSDVAIRIYHQFIIIMEKNIQPIIVPGMLEYESLQGI
                     SGLKPTGFRKRSSSIDDTDGYTMTSVLQQLSYFYTTMCQNGLDPELVRQAVKQLFFLIG
                     AVTLNSLFLRKDMCSCRKGMQIRCNISYLEEWLKDKNLQNSLAKETLEPLSQAAWLLQV
                     KKTTDSDAKEIYERCTSLSAVQIIKILNSYTPIDDFEKRVTPSFVRKVQALLNSREDSS
                     QLMLDTKYLFQVTFPFTPSPHALEMIQIPSSFKLGFLNRL"
                     /protein_id="NP_061198.2"
                     /label="MYO5C"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtcaagac
     1501 aatctctgag ttcgaaaaag agatagaact acttcaggca cagaagatag atgtggagaa
     1561 acatgtgcag tcacaaaaac gggaaatgag agaaaagatg tcagagatca ccaaacaact
     1621 tctcgaaagc tatgacattg aagatgtaag aagcaggctc tctgtggaag atctggaaca
     1681 tttaaatgag gatggagaac tttggtttgc ttatgaagga ctaaagaaag caacacgtgt
     1741 tttggagagc catttccagt ctcagaagga ttgctatgaa aaggagattg aagctttgaa
     1801 cttcaaagtg gtgcatctca gtcaagaaat caaccacctg cagaagttat tcagagaaga
     1861 aaatgacatc aatgaaagca tccgtcatga agttaccagg ctaacatcag agaacatgat
     1921 gatcccagac tttaaacagc aaatttcaga attggagaaa cagaagcaag atcttgaaat
     1981 ccgcctgaat gaacaagctg agaaaatgaa aggaaaacta gaagaattgt ctaatcagtt
     2041 acatcgcagt caagaggagg aaggaacaca aagaaaggcc ttggaagccc aaaatgaaat
     2101 acataccaaa gagaaggaga agctgattga taagattcaa gaaatgcagg aggccagtga
     2161 ccacttgaag aaacaatttg aaactgaaag tgaagtcaag tgtaacttcc ggcaggaagc
     2221 atcccggctc actttggaaa acagggatct tgaagaagaa ttagacatga aagacagagt
     2281 gattaaaaag ctacaagatc aagtcaagac actaagcaag acaattggaa aagccaatga
     2341 tgtgcactcg tcctcaggac ccaaggagta ccttggaatg ctgcaataca agagagaaga
     2401 cgaggccaag ctcattcaga acctcattct tgacttgaag ccccgtggcg tggtggtgaa
     2461 catgatcccc gggctgccgg ctcatatcct gttcatgtgt gtgcgctacg cagactctct
     2521 gaatgatgcc aacatgctga agtccctcat gaacagcacc attaatggca tcaagcaggt
     2581 ggttaaggaa catttagaag actttgaaat gctgtccttt tggctttcca acacttgtca
     2641 ttttctcaat tgcctgaagc agtacagcgg agaagaggaa ttcatgaagc ataatagtcc
     2701 acagcagaat aagaattgct tgaacaattt tgacctttca gaatacagac agattctcag
     2761 tgatgtggct atacgaatat atcatcaatt tattatcata atggaaaaga atatccaacc
     2821 gataatagtt ccgggaatgc tggagtatga gagcctgcag ggcatttccg gcctgaagcc
     2881 cacaggcttc cggaagcgct cctctagcat agacgacacg gacggctaca ccatgacctc
     2941 cgtcctgcaa cagctgagct acttttacac caccatgtgc cagaacggcc tggaccccga
     3001 gcttgtgagg caggcggtga agcagctctt cttcttgatc ggggcggtca cgctgaacag
     3061 cctcttcctg cgcaaggaca tgtgctcctg cagaaaaggg atgcagatca ggtgcaatat
     3121 cagctactta gaagaatggc ttaaagataa gaacttgcag aacagcttag caaaggaaac
     3181 tttggagccc ctctctcagg cagcctggtt gcttcaggtc aagaagacca cagacagtga
     3241 tgccaaggag atctacgaac gctgcacctc actgtctgct gtgcagatca taaagatcct
     3301 taattcatac acacctatag atgactttga gaagagagtg actccatcct ttgttcgcaa
     3361 agtacaggct ctcctaaata gccgggagga ttcatcacag ctgatgttgg ataccaaata
     3421 tctctttcaa gtcacatttc cttttacccc ctctccacat gctctggaaa tgattcagat
     3481 ccccagcagt ttcaagctag gctttctgaa taggttatgt acagactaca aagaccatga
     3541 cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagt aactcgagat
     3601 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     3661 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     3721 atggctttca ttttctcctc cttgtataaa tcctggttag ttcttgccac ggcggaactc
     3781 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     3841 gtggtgttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat ctagctttat
     3901 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     3961 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt
     4021 ttaaagatct ggagaccggt gtggcacgtg cggaccgagc ggccgcagga acccctagtg
     4081 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
     4141 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc
     4201 ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     4261 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4321 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4381 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     4441 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
     4501 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4561 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4621 cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     4681 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
     4741 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     4801 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     4861 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     4921 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     4981 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5041 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5101 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5161 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5221 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5281 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5341 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5401 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5461 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5521 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5581 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5641 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     5701 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     5761 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     5821 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     5881 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5941 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6001 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6061 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6121 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6181 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6241 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6301 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6361 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6421 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6481 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6541 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6601 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6661 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6721 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6781 tttgctggcc ttttgctcac atgt
//
