LOCUS       pL0M-PU-pLsUBI          4159 bp ds-DNA     circular     30-SEP-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..1411
                     /label="Promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    10..13
                     /label="GGAG\fusion\site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    50..50
                     /label="A > T mutation to destroy BsaI site"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1212..1212
                     /label="G > C mutation to remove BpiI site (may not be exact)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     regulatory      1379..1384
                     /label="regulatory"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1403..1403
                     /label="g > a mutation to destroy Bpi site"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     exon            1412..1464
                     /label="5' UTR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     intron          1465..1921
                     /label="intron"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1922..1925
                     /label="AATG\fusion\site"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             2152..3162
                     /label="Sm/Sp\no\DraIII"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     mutation        3129..3129
                     /label="G\to\A"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3322..3936
                     /label="rep\(pMB1)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      complement(3921..3921)
                     /label="ORI"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
ORIGIN
        1 gtggtctcag gaggaaacaa gtgtccgaaa tcctacttcg gtttttcagt gacctctcta
       61 aagtgatata ttcccgacaa aatggttatt ttacaaaaaa aataatcatt ttttttcaaa
      121 ttattcattt atatgggtta gaatactcaa tttccctgtg ttatttaatt taaaatcatt
      181 tccaatatac aaacatactt tattatctga ttatttataa taaattttca taaagtttca
      241 cctaatcttt tattccttat attataaaat ttatccatcc aaatcaaact tattttgtat
      301 aatttaaaag tattttcaat ataatcattt ggtttgtggt gacgtagtaa aacatatgaa
      361 atatatgatg tataacttat tgatcaatgt tcaaattttg acactactcg gtcctgtggc
      421 cataaatgtt tgtcggcatt gactccaaaa catgaagtat cctctttaat aaattaaagt
      481 ttttttttgt cacttgtcac actctcatgc attatacaaa atgtcatttt gtcataattt
      541 tgagatattt atttttcatt tgtcatttac ttcacttttt atttttgaat atatccttaa
      601 tttagttttt ataaattaac catatcataa taactatatt aattttaaat tttgaatttc
      661 aaacttcaat cgaaacttta aataaattta caattgaaac gtttgtattt aacattttgt
      721 tataattaga catttgatac acgagtcaga taactaaaca cataatttta atttattttt
      781 ttcatttttt ttttataatt cttacttatt ccaaatttca aattcaaata aatttgctgt
      841 ttaatcattc gtatttaaca ttttattgta atcaacccgt ataatataca agtatcacaa
      901 ctactaacct catatttgca gcgaaagatt attcggcgtg caaaagcagg gcaaaaaaac
      961 ccttgttaga aagtttcttt tccaaaataa aaaattaaaa aataaaagta ttttcaatat
     1021 caataacata ctctattctc taattattta ttactaactt ttcaaagaga ttaacctaat
     1081 cttgtattcc ttaaattata aaatttacaa attatatgga tccaaaacca aatatcccat
     1141 cgactcaaga tttaagttaa aagtttcttg gacgaaacac aaagaaaaga tattatccta
     1201 acccaaaaga acacggcagc cttagaaatc accgccactg attaaatatc ggaatccaac
     1261 gactctaatt tcagaccctt catcaacttt ttattgacac gtgtcatatt ttcgtccgtt
     1321 tgtatctaac aacccgtagc gttagccttc cttttcgtga agaaaccttt tcttatccta
     1381 taaatccacc cccaaatcca tcatcttcct tcatcctgca atcatcttta tcttcaaagc
     1441 tctgacttct tcaattctcc caaggtatta tcttttttat tcttttatgt ttgtttagtt
     1501 tttgaacagt ttattttcaa tcgattaaag gttattattg tttttgatct gtttgtggct
     1561 tttgatttaa gatataacct gatgttgtaa ttaacacgat tgatagtgtc ttaatatgtt
     1621 ttaattatgg tttctagatc taatcatcga atttattaac gatctgttat agtttcgtgt
     1681 tttcgatcgt ttttcaacaa gtcaattcca aaatcgatgt tattgtataa aagcttcgaa
     1741 cgacgatttt ttgtaaatta attcacgatc taagttttca atttcagaga ataaagttgt
     1801 tgatttgttt ttgaattgta atctgatctc tgtttcaagt acgatatgaa tttgtttgtt
     1861 gatgaattga tgataaaact gttttcgttt tctaataatt gtttcgttct ttttttaaca
     1921 gaatgtgaga ccacgaagtg gctcttcagt ggacgaaagg gcctcgtgat acgcctattt
     1981 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     2041 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     2101 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgcgctca
     2161 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     2221 atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa
     2281 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
     2341 ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg
     2401 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
     2461 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagcga tattgatttg
     2521 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
     2581 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
     2641 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
     2701 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
     2761 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
     2821 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
     2881 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
     2941 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
     3001 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
     3061 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
     3121 cattacgtaa aaggcgagat caccaaggta gtcggcaaat aactgtcaga ccaagtttac
     3181 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3241 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3301 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3361 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3421 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     3481 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3541 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3601 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3661 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3721 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3781 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3841 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3901 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3961 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     4021 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     4081 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     4141 ccgattcatt aatcactct
//