LOCUS       DoS2F-5                 4142 bp    DNA     circular SYN 01-AUG-2025
DEFINITION  DoS2F-5.
ACCESSION   DoS2F-5
VERSION     DoS2F-5
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     CDS             1745..2368
                     /note="pLannotate"
                     /label="TetR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             3281..3937
                     /note="pLannotate"
                     /label="CAT_ECOLX"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     oriT            3021..3130
                     /note="pLannotate"
                     /label="oriT"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="oriT"
     CDS             complement(924..1625)
                     /note="pLannotate"
                     /label="SCE1_YEAST"
                     /database="swissprot"
                     /identity="99.1"
                     /match_length="99.6"
                     /fragment="False"
                     /other="CDS"
     promoter        3178..3280
                     /note="pLannotate"
                     /label="cat promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     terminator      3961..4055
                     /note="pLannotate"
                     /label="lambda t0 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      complement(754..840)
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     rep_origin      complement(2460..2763)
                     /note="pLannotate"
                     /label="oriV (fragment)"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="57.1"
                     /fragment="True"
                     /other="rep_origin"
     terminator      complement(2390..2437)
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     promoter        complement(1671..1726)
                     /note="pLannotate"
                     /label="tetR/tetA promoters"
                     /database="snapgene"
                     /identity="98.2"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgccctagg tctagggcgg cggatttgtc ctactcagga gagcgttcac
      781 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
      841 atgcctctag attaattaat taagcggccg catcgatcgg gccctgaggc ctgcagggat
      901 ccattaccct gttatcccta ttatttcagg aaagtttcgg aggagatagt gttcggcagt
      961 ttgtacatca tctgcgggat caggtacggt ttgatcaggt tgtagaagat caggtaagac
     1021 atagaatcga tgtagatgat cggtttgttt ttgttgattt ttacgtaaca gttcagttgg
     1081 aatttgttac gcagaccctt aaccaggtat tctacttctt cgaaagtgaa agactgggtg
     1141 ttcagtacga tcgatttgtt ggtagagttt ttgttgtaat cccatttacc accatcatcc
     1201 atgaaccagt atgccagaga catcggggtc aggtagtttt caaccaggtt gttcgggatg
     1261 gtttttttgt tgttaacgat gaacaggtta gccagtttgt tgaaagcttg gtgtttgaaa
     1321 gtctgggcgc cccaggtgat taccaggtta cccaggtggt taacacgttc ttttttgtgc
     1381 ggcggggaca gtacccactg atcgtacagc agacatacgt ggtccatgta tgctttgttt
     1441 ttccactcga actgcataca gtaggtttta ccttcatcac gagaacggat gtaagcatca
     1501 cccaggatca gaccgatacc tgcttcgaac tgttcgatgt tcagttcgat cagctgggat
     1561 ttgtattctt tcagcagttt agagttcgga cccaggttca ttacctggtt tttttttgat
     1621 gtttttcata tgcatatagg taccctcctc tttagatctt ttgaattctt ttctctatca
     1681 ctgataggga gtggtaaaat aactctatca acgatagagt gtcaacaaaa attaggaatt
     1741 aatgatgtct agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga
     1801 ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc
     1861 tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat
     1921 gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt
     1981 acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt
     2041 acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt
     2101 tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca
     2161 ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga
     2221 aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca
     2281 ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa
     2341 acaacttaaa tgtgaaagtg ggtcttaagc atgcctcgag gtgaagacgc aaaaaacccc
     2401 tcaagacccg tttagaggcc ccaaggggtt atgctagcct cgtgatacgc ctaacgcgtg
     2461 acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc atcactctgc ccctcaagtg
     2521 tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg
     2581 cacttatccc caggcttgtc cacatcatct gtgggaaact cgcgtaaaat caggcgtttt
     2641 cgccgatttg cgaggctggc cagctccacg tcgccggccg aaatcgagcc tgcccctcat
     2701 ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt
     2761 cagacgtcga tatctggcga aaatgagacg ttgatcgtct ctcgcctgtc ccctcagttc
     2821 agtaatttcc tgcatttgcc tgtttccagt cggtagatat tccacaaaac agcagggaag
     2881 cagcgctttt ccgctgcata accctgcttc ggggtcatta tagcgatttt ttcggtatat
     2941 ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg
     3001 tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc gagcgggtgt
     3061 tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc tgctctgcga
     3121 ggctggccgg ctaccgccgg cgtaacagat gagggcaagc ggatggctgt gagacgttga
     3181 tcggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt
     3241 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     3301 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     3361 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     3421 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     3481 gaatgctcat ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag
     3541 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     3601 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     3661 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
     3721 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     3781 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     3841 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
     3901 attacaacag tactgcgatg agtggcaggg cggggcgtaa tttgatatcg agctcgcttg
     3961 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
     4021 tcggttgccg ccgggcgttt tttattggtg agaatccaag cactagtcat gaccaaaatc
     4081 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     4141 tc
//
