LOCUS       plasmid                 7419 bp    DNA     circular SYN 29-FEB-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     rep_origin      complement(2539..2967)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        complement(5639..6018)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     polyA_signal    complement(3013..3237)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(5435..5638)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(1338..2129)
                     /note="pLannotate"
                     /label="neo"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    complement(1040..1161)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        6284..6388
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(3517..5295)
                     /note="pLannotate"
                     /label="PTPN11"
                     /database="swissprot"
                     /identity="99.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             6389..7249
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(2196..2525)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    877..898
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(5372..5390)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        3263..3281
                     /note="pLannotate"
                     /label="SP6 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    951..967
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(1156..1314)
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="98.1"
                     /match_length="42.1"
                     /fragment="True"
                     /other="CDS"
     misc_feature    6173..6225
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="37.6"
                     /fragment="True"
                     /other="misc_feature"
     CDS             772..864
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     LTR             complement(5406..5439)
                     /note="pLannotate"
                     /label="5' LTR (truncated) (fragment)"
                     /database="snapgene"
                     /identity="97.1"
                     /match_length="18.8"
                     /fragment="True"
                     /other="LTR"
     promoter        complement(2988..3009)
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.1"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     1321 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     1381 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     1441 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     1501 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     1561 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     1621 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     1681 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     1741 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     1801 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     1861 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     1921 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     1981 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     2041 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     2101 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     2161 gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa
     2221 aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa
     2281 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     2341 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     2401 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     2461 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     2521 cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2581 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2641 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2701 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2761 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2821 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2881 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2941 cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt
     3001 ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
     3061 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
     3121 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
     3181 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
     3241 aggctgatca gcgagctcta gcatttaggt gacactatag aatagggccc tctagatgca
     3301 tgctcgagcg gccgccagtg tgatggatat ctgcagaatt ccggtcttta aatggtttca
     3361 aattttgctt atcaaaaggt agtcaaccac attgcaagcc ttccaaatcc aaattctgtc
     3421 ttcacataaa ctttcttgcg tctgttcttg atctttttag ggagagggtg aaagtccaca
     3481 tctatttctg tgctgaagtt ttggcaggtt ttctcatctg aaacttttct gctgttgcat
     3541 caggcccacg ttttcataga ctctagcact gtcttctctc atttctgcac agggtggcgt
     3601 tggagtacaa ggcgggagag ggctctgatc tccactcgtc tggtccgcta gagaatactt
     3661 aatatttgta tattcgtgcc ctttcctctt gcttttctgc tcttcttcaa tcctgcgctg
     3721 tagtgtttca atataatgct ggaccgccat atagataaat cggtactgtg cttctgtctg
     3781 gaccatccct gacctctgag accgcaccat ctggatggtt ttgggaacgt caatatcgca
     3841 gtcaacacct ttctctctga tgatgtcaat aagaatatca atcacaatga acgtccctgt
     3901 ccggccaatt ccagcactcg agtgcaccac gaccggccct gcatccatga tgctctcctg
     3961 cttatggtgc acctcctcca ggaagtccag cacgccccca gggtcgctgg gcacgccgtg
     4021 gtccggccag gtccgaaagt ggtattgcca gaccgttctc tccgtattcc cttgtccaac
     4081 ctttgaaagt ttaagttctc ttagcgtata gtcatgagcg gcgctttctt tgacgttcct
     4141 aacacgcatg acgccatatt cttttagagc atactcatca ggccagtatt tgacacattt
     4201 actctttcct ctctccactt ctttcgttgt catgacaatc actcgggagt tttcttggaa
     4261 caccatccgc caaaagtcat tcaccgtgtt ttgcaggcag ccttgtgtgg caatgtaact
     4321 ctttttgggc tttgaattgt tgcacttggt ttcaaattca ggcatgatga tatttgcatt
     4381 gatgtaatct gaaacaggct cattgggatc accatcgtgt aggacaaccc tggtatgatc
     4441 aaagggcagg atgtttttat atctattttt gtttttgttt tcttgccttt gaccctcttt
     4501 tcggctgtag agaagtttgc actcctgttg ttgtagtgtc gcaaattctt cccaaaagcc
     4561 ttgtttgact ttatctgtgg tctcagctaa tttgcttagt tctcgaactc tgctttctat
     4621 ttcagcagca tttatacgag tcgtgttaag gggctgcttg agttgtagta ctgtacccaa
     4681 tgtttccacc ataggattct tcttataatg ttccacaaga tctgtcaaag aatcaaaccg
     4741 ttctcctcca ccaacgtcgt atttcagttc ctgacagcga atcataacat gggtcacttt
     4801 agacttgccg tcattgctct cccctttgtc atcaccagtg cgcacagaaa gaacaaaatc
     4861 tccagggtgg ctctggctct ctcgtacaag aaaactacca tgttttcctt tttcagttaa
     4921 taatttctct gcttctttcc cagagagagc tccatgaaac cacctttcag aggtaggatc
     4981 tgcacagttc agaggatatt taagctcaat gacatctcca ttcttctctt ttaattgccc
     5041 gtgatgttcc atgtaatact ggaccaactc agccaaagtg gcaaatttct cccctccata
     5101 caggtcatag taatcaccag tgttctgaat cttgatgtgg gtgacagctc catttcttct
     5161 aacggaaagt gtgaagtctc cagggttact tttactaggc cttgccaaaa aactgccatc
     5221 aactcctctt gtcaacagta ggttttctgc ctccacacca gtgatatttg ggtgaaacca
     5281 tctccgcgat gtcatgttcc tcccacccgg aattccagca cactggcggc cgttactagt
     5341 ggatccgagc tcggtaccaa gcttgggtct ccctatagtg agtcgtatta atttcgataa
     5401 gccagtaagc agtgggttct ctagttagcc agagagctct gcttatatag acctcccacc
     5461 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
     5521 cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa
     5581 atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca
     5641 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
     5701 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
     5761 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
     5821 attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg
     5881 tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa
     5941 cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac tattaataac
     6001 tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc agcgcaaaac
     6061 gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt gttgtagctt
     6121 aaattttgct cgcgcactac tcagcgacct ccaacacaca agcagggagc agatactggc
     6181 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatagggga tcgggagatc
     6241 tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6301 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     6361 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     6421 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6481 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     6541 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6601 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     6661 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     6721 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6781 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6841 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6901 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6961 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     7021 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     7081 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     7141 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     7201 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     7261 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7321 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7381 ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
