LOCUS       pADH99-Cau             11734 bp    DNA     circular SYN 19-MAR-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11734)
  AUTHORS   Dimitrios
  TITLE     Direct Submission
  JOURNAL   Exported Jul 30, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
COMMENT     Sequence Label: pADH99Cau
FEATURES             Location/Qualifiers
     source          1..11734
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     rep_origin      complement(238..826)
                     /direction=LEFT
                     /label=ori
                     /note="color: #ffff00"
     CDS             complement(1000..1857)
                     /codon_start=1
                     /label=AmpR
                     /note="color: #ff6600"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2068..2107
                     /label=Fragment 1.FOR
                     /note="color: black; sequence: 
                     gcgtttaaaccgcctcaagcGGATTTGCAGCTGGTAAATC"
     misc_feature    2088..3534
                     /label=CauHIS1_US
                     /note="color: #ff99cc; direction: RIGHT"
     primer_bind     complement(3516..3568)
                     /label=Fragment 1.REV
                     /note="color: black; sequence: 
                     gaagttcctattctctagaaagtataggaacttcCCCAGATTTAGCGATACTC"
     primer_bind     3535..3588
                     /label=Fragment 2.FOR
                     /note="color: black; sequence: 
                     gaagttcctatactttctagagaataggaacttcCCAGGATTCTACGCGCATTG"
     protein_bind    3535..3568
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
                     /note="color: #000000; direction: RIGHT"
     primer_bind     3568..3588
                     /label=ENO1p_sF
                     /note="color: black; sequence: 
                     GCGAATTGAGGCCTGCATGCGGTACCCCAGGATTCTACGCGCATTG"
     primer_bind     3568..3588
                     /label=Fragment.FOR
                     /note="color: black; sequence: 
                     gcgaattgaggcctgcatgcggtaccCCAGGATTCTACGCGCATTG"
     promoter        3569..3946
                     /label=ENO1p
                     /note="color: #ffff00; direction: RIGHT"
     primer_bind     complement(3913..3966)
                     /label=Eno1p_sR
                     /note="color: black; sequence: 
                     ATACTATACTTTTTATCCATCCCGGGGATGAAAATTAAGTTTGGATAGGGAGG"
     primer_bind     complement(3913..3966)
                     /label=Fragment.REV
                     /note="color: black; sequence: 
                     atactatactttttatccatcccgggGATGAAAATTAAGTTTGGATAGGGAGG"
     primer_bind     complement(3923..3965)
                     /label=Fragment 2.REV
                     /note="color: black; sequence: 
                     tactatactttttatccatcCCCGGGGATGAAAATTAAGTTTG"
     gene            3947..8203
                     /label=CaCas9
                     /note="color: #0000ff; direction: RIGHT"
     primer_bind     complement(3974..3996)
                     /label=Cas9_chR
                     /note="color: black; sequence: CCCACAGAGTTAGTACCAATATC"
     primer_bind     complement(4102..4121)
                     /label=Check_pV1200_R
                     /note="color: black; sequence: CAGTTTCACCGGAGTCGAAC; added:
                     2024-12-10"
     terminator      8262..8513
                     /label=tCYC1
                     /note="color: #0000ff"
     promoter        8514..9061
                     /label=pMAL2
                     /note="color: #ffffff"
     CDS             9068..10339
                     /codon_start=1
                     /label=ScFLP
                     /note="color: #993366"
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     terminator      10340..10727
                     /label=tACT1
                     /note="color: #ffffff"
     misc_feature    10728..11573
                     /label=NAT 1of2
                     /note="color: #ff6600"
ORIGIN
        1 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
       61 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      121 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      181 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      241 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      301 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      361 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      421 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      481 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
      541 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
      601 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
      661 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
      721 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
      781 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
      841 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
      901 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
      961 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     1021 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     1081 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     1141 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     1201 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     1261 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     1321 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     1381 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     1441 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     1501 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     1561 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     1621 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     1681 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     1741 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     1801 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     1861 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     1921 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     1981 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     2041 cacgaggccc tttgacggca cggccacgcg tttaaaccgc ctcaagcgga tttgcagctg
     2101 gtaaatcttt cgagaagcac aatattgtat actacaaatt tcttttccac actgcagact
     2161 atctcccttc gcaacataaa agtcacacac acacacacat catacgtatc atttttatac
     2221 ctactcacga aaactaatat catcgtggaa tcccctgagc atgtctgagt ggatctctac
     2281 ttttcctggt tacactctgg cagtgtccag agtgaaaaaa attgtgggaa gcatcataag
     2341 aactggccct actcctcgcc atgttggtgt catcatggac ggtaacagaa ggtatgcaaa
     2401 aagtcacaaa atcgagttga aagaaggaca taatttgggg ttcgactcga tggccaacat
     2461 tctcgagtta ctctatgagt caggtgttga gtgtgccact gtctacgcat tttcaattga
     2521 gaatttcaaa agactgcgct acgaggtcga gtggttgatg gacttagcca aatccaaatt
     2581 ccagcaaata agtcaacaag gcgaactttg cgaaaagtat ggtatcaaga taaaaatctt
     2641 gggcaacgtt gctctattgc cgtctgatgt acaggcaatc cttgccgaga cagaaaggat
     2701 tacgaagaac aacacaagag ctgtgctcaa tatttgtttt ccttacactt ctagagatga
     2761 gatggccacg tctatgagaa gaattgtgat gctatcggaa aaggtcccat cgctttttat
     2821 cgatgaatct accattgagg actacttgta cactgcagac tcaccacctc ttgatatctt
     2881 ggttcgtacc agtggaactt acaggctatc ggacttcttg ttgtggcagg cagtgcccgc
     2941 ttcttgttcg atcatgttca gcaacaagtt gtggccagat ttcactgcat gggacatgat
     3001 caagattctt ctcacctgga gcttccacgt gtactggtat ggaaatggta atggcattac
     3061 tggtgggcca acttctaagc ccattcatcc tgcaaagtct atagaaaaag atctctctcc
     3121 cctgccgggc ttgtcttcat cgacgacagc ttatgagagg tttcaggata attcaactga
     3181 tagtgagaag gattcactag gcaaatcgga cgtggaggat gttacatcta atctgtccac
     3241 gacatcagat gtcgcaaagt agcgatacac tagcatacta ctataccaag acactttgca
     3301 tcaacacaca agaatctagc tcggacttat ataccctcac tgagacaatt accatggact
     3361 tcttagatta cacaatcctc ctgatgaaga ggaattttct ctaatcgcat caactctatc
     3421 aaagacctca agatagtgta agtttaatat tcctgcagat gttggggtat tttaggttga
     3481 ctcattacta cacgcagccc tgaaaaatgg ttatcgagta tcgctaaatc tggggaagtt
     3541 cctatacttt ctagagaata ggaacttccc aggattctac gcgcattgca actccgaggc
     3601 cgataccctt tgaccagtgg tcaattcgtc acaattggca acattttcat gatttggcag
     3661 cttcacgttg aacaattgaa ccagcagaat ccagcaaaac gagtttccgt tgtgaatgcc
     3721 cctttgtgtg tgcaaataat catggtggct ccctaatggc ataccctcag tcacctggag
     3781 gaattgctcg ctcgagtctc ctgaggtgtc ctcctgcctg tggaaaaagc ggccaggtgt
     3841 ggtccaccgt tggaacagct tgctgtataa ataccacagg tgcgcacgcc ccaggggcct
     3901 tttttttttt ctcctcccta tccaaactta attttcatcc ccggggatgg ataaaaagta
     3961 tagtattggt ttagatattg gtactaactc tgtgggttgg gcagttatca ctgacgaata
     4021 taaagttcca tcaaagaaat ttaaggtgtt aggtaacact gacagacact caataaaaaa
     4081 gaatcttatc ggtgctcttt tgttcgactc cggtgaaact gccgaggcta cacgtttaaa
     4141 aagaacagca agaagaagat atacccgtag aaaaaataga atatgttatt tacaagaaat
     4201 cttttctaat gaaatggcta aagttgatga ttcctttttc catagattgg aagagtcatt
     4261 tttggttgaa gaagacaaaa agcatgagag acatccaatc tttgggaata tagttgatga
     4321 agtggcttac catgaaaaat atcctaccat ttatcattta agaaagaaat tggtagattc
     4381 aactgataaa gctgacctta gattaatcta tttagcactt gcccatatga ttaaatttag
     4441 aggtcatttt ttgattgaag gtgatttgaa cccagataat tctgacgtgg ataaattatt
     4501 tattcaatta gtccaaacct acaaccaatt atttgaggaa aatccaatta atgctagtgg
     4561 tgtcgatgcc aaagctatat tatcagccag attatcaaaa tctagacgtt tggaaaattt
     4621 gattgcccaa ttgccaggag aaaaaaagaa tggattattt ggaaacttga tcgcattatc
     4681 attgggtttg acaccaaatt ttaaatctaa ttttgattta gctgaagatg ctaaattaca
     4741 attatcaaaa gacacctatg acgacgattt ggacaattta cttgctcaaa ttggtgatca
     4801 atatgcagat ttgttcttag ctgctaaaaa cttatctgat gctattttgt tgtctgatat
     4861 tttgagagtg aacacagaaa taaccaaagc tccattatca gcatctatga tcaaacgtta
     4921 tgatgaacac catcaggatt tgactttatt gaaagctttg gtgagacaac aattgccaga
     4981 gaagtataaa gaaatctttt tcgatcaatc taaaaacggg tatgcaggtt atattgatgg
     5041 gggtgcctcc caagaggaat tttacaaatt tataaaacct attttagaaa agatggatgg
     5101 gactgaggaa cttttggtca aattgaacag agaagatttg ttacgtaaac agagaacttt
     5161 tgataatggt agtatacctc accaaattca tttgggtgag ttgcatgcaa ttttaagaag
     5221 acaagaagat ttttatccat ttttaaaaga taatagagaa aaaatcgaga aaattttaac
     5281 ctttagaatt ccatactatg ttgggccttt ggctagaggt aattcaagat ttgcctggat
     5341 gacacgtaaa tcagaagaaa ctattacccc ttggaatttt gaagaggttg ttgataaagg
     5401 agcatcagca cagagtttta ttgaaagaat gaccaatttc gataaaaact taccaaatga
     5461 aaaagtttta ccaaaacatt ccttgttata cgaatatttt actgtttaca atgaacttac
     5521 aaaggttaaa tatgttactg aaggtatgcg taagccagcc tttttatctg gagaacagaa
     5581 aaaggcaata gttgatttat tgtttaaaac aaatagaaaa gttactgtta aacaattaaa
     5641 agaagattac tttaagaaaa ttgaatgttt tgattcagtt gaaatcagtg gtgttgaaga
     5701 cagatttaat gctagtttag gaacttacca tgatttactt aaaattatca aagataaaga
     5761 tttcttggat aacgaagaaa atgaagacat tttagaagac attgttttaa ccttaacttt
     5821 attcgaagat agagagatga ttgaagaacg tttgaagact tatgcacatt tgtttgacga
     5881 taaagtgatg aaacagttga aaagaagacg ttatactgga tggggtagat tgtctcgtaa
     5941 attgatcaat ggaattagag ataaacaaag tggtaaaact atcttggact ttttgaaatc
     6001 tgacggattt gctaatagaa atttcatgca attgatccac gacgatagtt tgacatttaa
     6061 agaagacatc caaaaggccc aagtgagtgg gcaaggtgat tcattacatg aacatattgc
     6121 aaatttagcc ggatctcctg ctattaagaa agggatatta caaactgtta aagttgtgga
     6181 tgaattagtg aaagtaatgg gaagacataa acctgaaaac attgtcattg agatggcaag
     6241 agaaaatcaa actacacaaa aaggacagaa aaatagtaga gaacgtatga aaagaataga
     6301 agagggtatt aaagaattgg gtagtcaaat attgaaagaa cacccagtgg aaaataccca
     6361 gttgcaaaat gaaaaattat atctttacta ccttcaaaat ggacgtgata tgtatgttga
     6421 tcaggaatta gatataaata gactttcaga ttatgatgta gatcatatag ttccacaatc
     6481 tttcttgaaa gatgattcca tagacaataa agtattaact agaagtgata aaaatagagg
     6541 taaaagtgat aatgtcccaa gtgaggaagt cgtcaaaaag atgaaaaatt actggcgtca
     6601 acttttgaat gctaaattaa ttactcaaag aaaatttgat aatttgacta aagcagaaag
     6661 aggtgggctt tctgaattag ataaagccgg gttcattaaa agacaattgg tcgaaactag
     6721 acaaattact aaacatgttg cccaaatttt agattcccgt atgaacacta agtatgacga
     6781 aaatgataag ttaatacgtg aggttaaagt cattacttta aaatcaaaac ttgtctctga
     6841 tttcagaaag gatttccaat tctataaagt tagagaaatt aataattatc atcatgctca
     6901 tgatgcatat ttgaatgctg tagttggaac tgctttaatc aagaaatacc ctaaattaga
     6961 atctgaattt gtatatggtg attacaaagt ctatgatgtt agaaagatga ttgctaaatc
     7021 agaacaagaa attggtaaag ctacagctaa atacttcttt tactctaaca ttatgaattt
     7081 ctttaaaaca gaaattactt tggcaaacgg tgaaattaga aaaagacctc ttattgaaac
     7141 aaatggtgag actggagaga tagtttggga caaagggcgt gatttcgcta ctgttagaaa
     7201 agttttatca atgccacaag ttaacattgt aaagaaaaca gaggttcaaa ctggtggttt
     7261 ctcaaaagaa agtattttgc ctaaaagaaa tagtgataaa ttgattgcca gaaaaaagga
     7321 ttgggatcca aagaaatatg gtggtttcga ctcaccaacc gtagcctatt ctgttttggt
     7381 tgtggcaaag gttgaaaagg gtaaaagtaa aaagcttaaa tcagtaaaag aacttttggg
     7441 tattacaata atggaaagaa gttcctttga aaagaaccct attgattttt tggaagctaa
     7501 aggttataag gaagtaaaga aggacttaat aatcaaattg cctaaatatt ctttatttga
     7561 attagaaaat gggagaaaaa gaatgttggc ttctgctgga gaattgcaaa agggtaatga
     7621 attagcattg ccttccaaat atgttaactt cttgtattta gcttcacact atgaaaagtt
     7681 gaaagggtca ccagaagata acgagcaaaa acaattattt gttgaacaac acaaacacta
     7741 cttagatgag attatagaac aaattagtga attcagtaaa agagtgatat tagctgatgc
     7801 aaatttagat aaagttttgt cagcctataa caaacataga gataagccaa ttagagaaca
     7861 agcagaaaac attattcact tatttaccct taccaattta ggagcacctg ctgctttcaa
     7921 gtattttgat acaacaattg atcgtaaaag atatacctca acaaaagaag tcttagacgc
     7981 caccttaatt catcaatcaa tcactggatt gtatgagaca agaattgatt tgtctcaatt
     8041 gggtggtgat gaaggggctg atcctaagaa gaaaagaaaa gttgatccaa agaaaaagcg
     8101 taaggtggat cctaagaaaa agagaaaggt tgactacaaa gaccatgacg gtgattataa
     8161 agatcatgac atcgactaca aggatgacga tgacaagtga taatgactgc agagatccat
     8221 cgacctgccg ccaagctaat tccgggcgaa tttctgtcga gtcatgtaat tagttatgtc
     8281 acgcttacat tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca
     8341 acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt
     8401 tatatttcaa atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact
     8461 gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct ttaatttgcg gccccaataa
     8521 tgattggttt gatatttttg tctagtacca tctgtaccat tacacttaaa ttatctttat
     8581 atctgtctaa ctcgactgtc tggatttcat tgatgtagtc gtatgcatcg ttagttccaa
     8641 aaaatattgt catcaatttg atattggttt ccgactctaa aatttttgga agaatttgtc
     8701 tagcgtgctc tgagttgtag ccactgaaac cacggttaat aacatccaat tttcggatat
     8761 acacattctg taatgctgga tgaaagccat actgggtaca actaaactgg gtgatggagt
     8821 caccgaacaa cacaaattta ccgtattcca tgattgctat ggttgagaat tttttttttt
     8881 tcttgtccca cgccattttt caaattatgc agttgagaat gttagttttt gtgtacaccc
     8941 cgttcgctga atatttcgga ataattcaaa gattggggag tgggggaggc gatagacgaa
     9001 gacacggtat aaaaatgggc aaaattttcc ccaacttttt gcagtggttt aactaataat
     9061 cgtcgacatg ccacaatttg atatattatg taaaacacca cctaaggtgc ttgttcgtca
     9121 gtttgtggaa aggtttgaaa gaccttcagg tgagaaaata gcattatgtg ctgctgaact
     9181 aacctattta tgttggatga ttacacataa cggaacagca atcaagagag ccacattcat
     9241 gagctataat actatcataa gcaattcgtt gagtttcgat attgtcaata aatcactcca
     9301 gtttaaatac aagacgcaaa aagcaacaat tttggaagcc tcattaaaga aattgattcc
     9361 tgcttgggaa tttacaatta ttccttacta tggacaaaaa catcaatctg atatcactga
     9421 tattgtaagt agtttgcaat tacagttcga atcatcggaa gaagcagata agggaaatag
     9481 ccacagtaaa aaaatgctta aagcacttct aagtgagggt gaaagcatct gggagatcac
     9541 tgagaaaata ctaaattcgt ttgagtatac ttcgagattt acaaaaacaa aaactttata
     9601 ccaattcctc ttcctagcta ctttcatcaa ttgtggaaga ttcagcgata ttaagaacgt
     9661 tgatccgaaa tcatttaaat tagtccaaaa taagtatttg ggagtaataa tccagtgttt
     9721 agtgacagag acaaagacaa gcgttagtag gcacatatac ttctttagcg caaggggtag
     9781 gatcgatcca cttgtatatt tggatgaatt tttgaggaat tctgaaccag tcctaaaacg
     9841 agtaaatagg accggcaatt cttcaagcaa taaacaggaa taccaattat taaaagataa
     9901 cttagtcaga tcgtacaata aagctttgaa gaaaaatgcg ccttattcaa tctttgctat
     9961 aaaaaatggc ccaaaatctc acattggaag acatttgatg acctcatttc tttcaatgaa
    10021 gggcctaacg gagttgacta atgttgtggg aaattggagc gataagcgtg cttctgccgt
    10081 ggccaggaca acgtatactc atcagataac agcaatacct gatcactact tcgcactagt
    10141 ttctcggtac tatgcatatg atccaatatc aaaggaaatg atagcattga aggatgagac
    10201 taatccaatt gaggagtggc agcatataga acagctaaag ggtagtgctg aaggaagcat
    10261 acgatacccc gcatggaatg ggataatatc acaggaggta ctagactacc tttcatccta
    10321 cataaataga cgcatataag agtgaaattc tggaaatctg gaaatctggt tttgtattct
    10381 tgttattctt ctttttgtta ttacatatat aacttgttac ttttttaaaa aaatctttgt
    10441 atattttata aatatataaa actaaattta agaaaaagag aaaaatgttt tatttgagag
    10501 attgaaattt tacttgaatt tagcttagct tttataaagt attattatgt aaaaaaacaa
    10561 aacaaatata cattaaaaag ttaagactat aaaatagcca cccaaggcat ttctatatct
    10621 tgttgttgtt gttttcatct tctgtatcag aggaacttat tttattattt tcgtcacggg
    10681 tattttctct tgtttgatga ttcatcccat tcattccatc ataaaatatc aagcttgcct
    10741 cgtccccgcc gggtcacccg gccagcgaca tggaggccca gaataccctc cttgacagtc
    10801 ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc
    10861 cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga agcaaaaatt
    10921 acggctcctc gctgcagacc tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt
    10981 gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca ctgaggttct
    11041 tctttcatat acttcctttt aaaatcttgc taggatacag ttctcacatc acatccgaac
    11101 ataaacaacc ggatcctaca attatgtcta ctactttgga tgatactgct tatagataca
    11161 gaacttctgt tccaggtgat gctgaagcta ttgaagcttt ggatggttct ttcactaccg
    11221 atactgtttt tagagttact gctactggtg atggtttcac tttgagagaa gttccagttg
    11281 atccaccatt gactaaggtt ttcccagatg atgaatccga tgatgaatcc gatgctggtg
    11341 aagatggtga tccagattct agaactttcg ttgcttatgg tgatgatggt gatttggctg
    11401 gtttcgttgt tgtttcttat tctggttgga acagaagatt gactgttgaa gatattgaag
    11461 ttgctccaga acatagaggt catggtgttg gtagagcttt gatgggtttg gctactgaat
    11521 tcgccagaga aagaggtgct ggtcatttgt ggttggaagt taccaatgtt aatcggtgtt
    11581 taaaccccag cgcctggcgg gcgctcacaa ttccacacaa catacgagcc ggaagcataa
    11641 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
    11701 tgcccgcttt ccagtcggga aacctgtcgt gcca
//
