LOCUS       Plasmid_-_pBSII-LA-mTagBFP(AM)        4685 bp    DNA     circular UNA 25-NOV-2024
DEFINITION  Restriction Cloning ligation product.
ACCESSION   urn.local.frstrobl.883-gv4326z
VERSION     urn.local.frstrobl.883-gv4326z
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        626..645
                     /modified_by="frstrobl"
                     /label="T7 Promoter"
     ORF             723..773
                     /modified_by="frstrobl"
                     /label="LA ORF"
     misc_feature    774..781
                     /modified_by="frstrobl"
                     /label="NotI"
                     /note="Geneious type: Important RE Site"
     ORF             783..1493
                     /label="mTagBFP2(AM) ORF"
     misc_feature    1494..1501
                     /modified_by="frstrobl"
                     /label="SbfI"
                     /note="Geneious type: Important RE Site"
     polyA_signal    1502..2478
                     /modified_by="frstrobl"
                     /label="SV40 Poly(A)"
     rep_origin      2923..3537
                     /modified_by="frstrobl"
                     /label="Origin of Replication"
     ORF             complement(3697..4557)
                     /modified_by="frstrobl"
                     /label="Beta-Lactamase (AmpR) ORF"
     promoter        complement(4558..4662)
                     /modified_by="frstrobl"
                     /label="Beta-Lactamase Promoter"
ORIGIN      
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccgggca
      721 aaatgggcgt ggccgacttg attaaaaaat tcgaatcgat ttcgaaagaa gaagcggccg
      781 ccatgtcgaa aggtgaagaa ttgattaaag aaaacatgca tatgaaattg tacatggaag
      841 gcaccgtgga caaccaccac ttcaaatgca cctcggaagg cgaaggcaaa ccatacgaag
      901 gcacccaaac aatgagaatt aaagtggtgg aaggtggccc attgccattc gccttcgaca
      961 ttttggccac ctcgttcttg tacggatcga aaaccttcat taaccacacc caaggcattc
     1021 cagatttctt caaacaatcg ttcccagaag gcttcacctg ggaaagagtg acaacctacg
     1081 aagacggagg agtgttgacc gccacccaag acacctcgtt gcaagacgga tgcttgattt
     1141 acaacgtgaa aattagagga gtgaacttca catcgaatgg cccagtgatg caaaagaaaa
     1201 ccttgggttg ggaagccttc accgaaacct tgtacccagc cgatggtggt ttggaaggca
     1261 gaaacgacat ggcgttgaaa ttggtgggcg gctcgcactt gattgccaac gccaaaacca
     1321 cctacagatc gaaaaaaccg gccaaaaact tgaaaatgcc aggtgtgtac tacgtggact
     1381 acagattgga aagaattaaa gaagccaaca acgaaaccta cgtggaacaa catgaagtgg
     1441 cggtggcgag atactgcgac ttgccgtcga aattgggcca caaattgaac taacctgcag
     1501 ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     1561 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     1621 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     1681 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     1741 tccccaggaa gctcctctgt gtcctcataa accctaacct cctctacttg agaggacatt
     1801 ccaatcatag gctgcccatc caccctctgt gtcctcctgt taattaggtc acttaacaaa
     1861 aaggaaattg ggtaggggtt tttcacagac cgctttctaa gggtaatttt aaaatatctg
     1921 ggaagtccct tccactgctg tgttccagaa gtgttggtaa acagcccaca aatgtcaaca
     1981 gcagaaacat acaagctgtc agctttgcac aagggcccaa caccctgctc atcaagaagc
     2041 actgtggttg ctgtgttagt aatgtgcaaa acaggaggca cattttcccc acctgtgtag
     2101 gttccaaaat atctagtgtt ttcattttta cttggatcag gaacccagca ctccactgga
     2161 taagcattat ccttatccaa aacagccttg tggtcagtgt tcatctgctg actgtcaact
     2221 gtagcatttt ttggggttac agtttgagca ggatatttgg tcctgtagtt tgctaacaca
     2281 ccctgcagct ccaaaggttc cccaccaaca gcaaaaaaat gaaaatttga cccttgaatg
     2341 ggttttccag caccattttc atgagttttt tgtgtccctg aatgcaagtt taacatagca
     2401 gttaccccaa taacctcagt tttaacagta acagcttccc acatcaaaat atttccacag
     2461 gttaagtcct catttaaaga gctccagctt ttgttccctt tagtgagggt taattgcgcg
     2521 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     2581 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     2641 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     2701 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     2761 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     2821 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2881 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2941 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3001 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3061 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3121 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3181 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3241 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3301 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3361 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3421 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3481 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3541 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3601 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3661 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3721 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3781 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3841 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3901 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3961 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     4021 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4081 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4141 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4201 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4261 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     4321 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4381 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     4441 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     4501 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     4561 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     4621 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     4681 gccac
//
