LOCUS       Plasmid_-_pBSII-NLS-mEmerald(AM)        4664 bp    DNA     circular UNA 31-JUL-2025
DEFINITION  Restriction Cloning ligation product.
ACCESSION   urn.local.frstrobl.87j-gv4326q
VERSION     urn.local.frstrobl.87j-gv4326q
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        626..645
                     /modified_by="frstrobl"
                     /label="T7 Promoter"
     ORF             723..749
                     /modified_by="frstrobl"
                     /label="NLS ORF"
     misc_feature    750..757
                     /modified_by="frstrobl"
                     /label="NotI"
                     /note="Geneious type: Important RE Site"
     ORF             759..1472
                     /modified_by="frstrobl"
                     /label="mEmerald(AM) ORF"
     misc_feature    1473..1480
                     /modified_by="frstrobl"
                     /label="SbfI"
                     /note="Geneious type: Important RE Site"
     polyA_signal    1481..2457
                     /modified_by="frstrobl"
                     /label="SV40 Poly(A)"
     rep_origin      2902..3516
                     /modified_by="frstrobl"
                     /label="Origin of Replication"
     ORF             complement(3676..4536)
                     /modified_by="frstrobl"
                     /label="Beta-Lactamase (AmpR) ORF"
     promoter        complement(4537..4641)
                     /modified_by="frstrobl"
                     /label="Beta-Lactamase Promoter"
ORIGIN      
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccgggca
      721 aaatggcccc caagaagaaa aggaaagtgg cggccgcctc gaaaggcgaa gaattgttca
      781 ccggcgtggt gccaattttg gtggaattgg acggcgacgt gaacggccac aaattctcgg
      841 tgtcgggcga aggcgaaggc gacgccacct acggcaaatt gaccttgaaa ttcatttgta
      901 ccaccggcaa attgccagtg ccatggccaa ccttggtgac caccttgacc tacggcgtgc
      961 aatgtttcgc cagataccca gaccacatga aacaacacga cttcttcaaa tcggccatgc
     1021 cagaaggcta cgtgcaagaa agaaccattt tcttcaaaga cgacggcaac tacaaaacca
     1081 gagccgaagt gaaattcgaa ggcgacacct tggtgaacag gattgaattg aaaggcattg
     1141 acttcaaaga agatggcaac attttgggcc acaaattgga atacaactac aactcgcaca
     1201 aagtgtacat taccgccgac aaacaaaaga acggcattaa agtgaacttc aaaaccagac
     1261 acaacattga agatggctcg gtgcaattgg ccgaccacta ccaacaaaac accccaattg
     1321 gcgacggccc agtgttgttg ccagacaacc actacttgtc gacccaatcg aaattgtcga
     1381 aagacccaaa cgaaaagagg gaccacatgg tgttgttgga attcgtgacc gccgccggca
     1441 ttaccttggg catggacgaa ttgtacaaat aacctgcagg gatcataatc agccatacca
     1501 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     1561 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     1621 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     1681 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccccaggaag ctcctctgtg
     1741 tcctcataaa ccctaacctc ctctacttga gaggacattc caatcatagg ctgcccatcc
     1801 accctctgtg tcctcctgtt aattaggtca cttaacaaaa aggaaattgg gtaggggttt
     1861 ttcacagacc gctttctaag ggtaatttta aaatatctgg gaagtccctt ccactgctgt
     1921 gttccagaag tgttggtaaa cagcccacaa atgtcaacag cagaaacata caagctgtca
     1981 gctttgcaca agggcccaac accctgctca tcaagaagca ctgtggttgc tgtgttagta
     2041 atgtgcaaaa caggaggcac attttcccca cctgtgtagg ttccaaaata tctagtgttt
     2101 tcatttttac ttggatcagg aacccagcac tccactggat aagcattatc cttatccaaa
     2161 acagccttgt ggtcagtgtt catctgctga ctgtcaactg tagcattttt tggggttaca
     2221 gtttgagcag gatatttggt cctgtagttt gctaacacac cctgcagctc caaaggttcc
     2281 ccaccaacag caaaaaaatg aaaatttgac ccttgaatgg gttttccagc accattttca
     2341 tgagtttttt gtgtccctga atgcaagttt aacatagcag ttaccccaat aacctcagtt
     2401 ttaacagtaa cagcttccca catcaaaata tttccacagg ttaagtcctc atttaaagag
     2461 ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
     2521 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     2581 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     2641 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     2701 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     2761 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2821 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2881 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2941 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3001 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3061 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3121 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3181 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3241 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3301 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     3361 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3421 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3481 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3541 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3601 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3661 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3721 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     3781 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     3841 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3901 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3961 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     4021 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4081 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4141 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     4201 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     4261 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     4321 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     4381 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     4441 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     4501 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     4561 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4621 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac
//
