LOCUS       Plasmid_-_pBSII-MEME-mEmerald(AM)        4733 bp    DNA     circular UNA 31-JUL-2025
DEFINITION  Restriction Cloning ligation product.
ACCESSION   urn.local.frstrobl.87g-gv4326q
VERSION     urn.local.frstrobl.87g-gv4326q
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        626..645
                     /modified_by="frstrobl"
                     /label="T7 Promoter"
     ORF             723..818
                     /modified_by="frstrobl"
                     /label="MEME ORF"
     misc_feature    819..826
                     /modified_by="frstrobl"
                     /label="NotI"
                     /note="Geneious type: Important RE Site"
     ORF             828..1541
                     /modified_by="frstrobl"
                     /label="mEmerald(AM) ORF"
     misc_feature    1542..1549
                     /modified_by="frstrobl"
                     /label="SbfI"
                     /note="Geneious type: Important RE Site"
     polyA_signal    1550..2526
                     /modified_by="frstrobl"
                     /label="SV40 Poly(A)"
     rep_origin      2971..3585
                     /modified_by="frstrobl"
                     /label="Origin of Replication"
     ORF             complement(3745..4605)
                     /modified_by="frstrobl"
                     /label="Beta-Lactamase (AmpR) ORF"
     promoter        complement(4606..4710)
                     /modified_by="frstrobl"
                     /label="Beta-Lactamase Promoter"
ORIGIN      
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccgggca
      721 aaatgttgtg ctgcatgagg aggaccaaac aagtggaaaa gaatgatgag gaccaaaaaa
      781 ttggctcggc cggctcggcc gccggctcgg gcgaattcgc ggccgcctcg aaaggcgaag
      841 aattgttcac cggcgtggtg ccaattttgg tggaattgga cggcgacgtg aacggccaca
      901 aattctcggt gtcgggcgaa ggcgaaggcg acgccaccta cggcaaattg accttgaaat
      961 tcatttgtac caccggcaaa ttgccagtgc catggccaac cttggtgacc accttgacct
     1021 acggcgtgca atgtttcgcc agatacccag accacatgaa acaacacgac ttcttcaaat
     1081 cggccatgcc agaaggctac gtgcaagaaa gaaccatttt cttcaaagac gacggcaact
     1141 acaaaaccag agccgaagtg aaattcgaag gcgacacctt ggtgaacagg attgaattga
     1201 aaggcattga cttcaaagaa gatggcaaca ttttgggcca caaattggaa tacaactaca
     1261 actcgcacaa agtgtacatt accgccgaca aacaaaagaa cggcattaaa gtgaacttca
     1321 aaaccagaca caacattgaa gatggctcgg tgcaattggc cgaccactac caacaaaaca
     1381 ccccaattgg cgacggccca gtgttgttgc cagacaacca ctacttgtcg acccaatcga
     1441 aattgtcgaa agacccaaac gaaaagaggg accacatggt gttgttggaa ttcgtgaccg
     1501 ccgccggcat taccttgggc atggacgaat tgtacaaata acctgcaggg atcataatca
     1561 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     1621 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     1681 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     1741 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cccaggaagc
     1801 tcctctgtgt cctcataaac cctaacctcc tctacttgag aggacattcc aatcataggc
     1861 tgcccatcca ccctctgtgt cctcctgtta attaggtcac ttaacaaaaa ggaaattggg
     1921 taggggtttt tcacagaccg ctttctaagg gtaattttaa aatatctggg aagtcccttc
     1981 cactgctgtg ttccagaagt gttggtaaac agcccacaaa tgtcaacagc agaaacatac
     2041 aagctgtcag ctttgcacaa gggcccaaca ccctgctcat caagaagcac tgtggttgct
     2101 gtgttagtaa tgtgcaaaac aggaggcaca ttttccccac ctgtgtaggt tccaaaatat
     2161 ctagtgtttt catttttact tggatcagga acccagcact ccactggata agcattatcc
     2221 ttatccaaaa cagccttgtg gtcagtgttc atctgctgac tgtcaactgt agcatttttt
     2281 ggggttacag tttgagcagg atatttggtc ctgtagtttg ctaacacacc ctgcagctcc
     2341 aaaggttccc caccaacagc aaaaaaatga aaatttgacc cttgaatggg ttttccagca
     2401 ccattttcat gagttttttg tgtccctgaa tgcaagttta acatagcagt taccccaata
     2461 acctcagttt taacagtaac agcttcccac atcaaaatat ttccacaggt taagtcctca
     2521 tttaaagagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     2581 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     2641 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     2701 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     2761 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     2821 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     2881 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     2941 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3001 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3061 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3121 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3181 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3241 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3301 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3361 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3421 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3481 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3541 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3601 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     3661 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3721 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     3781 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     3841 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     3901 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     3961 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4021 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4081 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4141 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4201 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4261 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4321 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     4381 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4441 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     4501 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4561 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     4621 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     4681 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac
//
